AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesTechnology, Industry, AgricultureInformation Science
Aurora KinasesMitosisAurora Kinase BAurora Kinase AAurora Kinase CProtein-Serine-Threonine KinasesSpindle ApparatusChromosome SegregationKinetochoresCell Cycle ProteinsCentrosomeMicrotubulesAnaphaseCDC2 Protein KinaseCell CycleHeLa CellsCytokinesisTelophaseMicrotubule-Associated ProteinsChromosomal Proteins, Non-HistoneCentromereMetaphaseInterphaseM Phase Cell Cycle CheckpointsChromosomesPhosphorylationCyclin BG2 PhaseKinesinNocodazoleNuclear ProteinsUbiquitin-Protein Ligase ComplexesAnaphase-Promoting Complex-CyclosomePolyploidyXenopus ProteinsCyclin B1SchizosaccharomycesChromatidsChromosomes, HumanMeiosisCell DivisionHistonesRNA InterferenceMad2 ProteinsSchizosaccharomyces pombe ProteinsTubulinProphaseProtein Kinase InhibitorsProtein KinasesCell NucleusCell Line, TumorMolecular Sequence DataCdc20 ProteinsMutationAmino Acid SequenceMicroscopy, FluorescenceOrganophosphatesMitosis ModulatorsG2 Phase Cell Cycle CheckpointsTubulin ModulatorsRNA, Small InterferingSaccharomyces cerevisiae ProteinsCell Nucleus DivisionCell Cycle CheckpointsGenes, cdcProtein BindingCell LineChromatincdc25 PhosphatasesAneuploidyNuclear EnvelopeInhibitor of Apoptosis ProteinsXenopusS PhaseFungal ProteinsAzepinesChromosomal InstabilityPotoroidaeModels, BiologicalRecombinant Fusion ProteinsCentriolesMultiprotein ComplexesFluorescent Antibody TechniqueG1 PhaseSaccharomyces cerevisiaeDrosophila ProteinsDNA ReplicationProtein TransportCell ExtractsApoptosisSaccharomycetalesNuclear Matrix-Associated ProteinsCell ProliferationGreen Fluorescent ProteinsCdh1 ProteinsXenopus laevisHCT116 Cellsran GTP-Binding ProteinBenzamidesEndoreduplication
CytokinesisSerineChromosomesInhibitionMitotic spindleApoptosisCentrosomeKinases playMicrotubulesPolesPhosphorylationPathwayChromosome segregationProteinsInducesSmall-moleculeNIMAMolecularAnaphaseLocalizesDrosophilaRNAiRegulationSegregationInhibitor of auroraAURKAProgressionKinase domainRegulatesInhibitorsGeneMetaphaseCellINCENPHeLaMicrotubuleComplexCellsPromotesUnderstoodSelectiveStagesRoleCentrosomesCentromeresCrucialPassenger
Cytokinesis11
Serine8
  • Aurora kinase A also known as serine/threonine-protein kinase 6 is an enzyme that in humans is encoded by the AURKA gene. (wikipedia.org)
  • Aurora A is a member of a family of mitotic serine/threonine kinases. (wikipedia.org)
  • Further studies demonstrated that CPAP directly interacts with and is phosphorylated by Aurora-A at serine 467 during mitosis. (sinica.edu.tw)
  • A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. (lookformedical.com)
  • Mammalian aurora-A belongs to a multigenic family of mitotic serine/threonine kinases comprising two other members: aurora-B and aurora-C. In this review we will focus on aurora-A that starts to localize to centrosomes only in S phase as soon as centrioles have been duplicated, the protein is then degraded in early G1. (lookformedical.com)
  • A family of serine/threonine kinase Aurora constitutes a key regulator in the orchestration of mitotic events. (lookformedical.com)
  • Aurora kinases constitute a family of serine-threonine kinases that are strongly associated with cancer. (cogershop.com)
  • Phosphorylation of Histone H3 on serine 10, a known Aurora B cellular target, is inhibited with an EC50 of ~9nM following treatment of cells with SNS-314. (cogershop.com)
Chromosomes9
  • The Aurora family of kinases and their downstream targets are key components of the cellular machinery that ensures the proper execution of mitosis and the accurate segregation of chromosomes to daughter cells. (cornell.edu)
  • Aurora A also assures proper organization and alignment of the chromosomes during prometaphase. (wikipedia.org)
  • As ATR is activated at centromeres, it stimulates Aurora B through Chk1, preventing formation of lagging chromosomes. (umn.edu)
  • This stage of mitosis is also an important one given that it's the stage at which the chromosomes start aligning at the central part of the cell so that they can be divided. (microscopemaster.com)
  • Mitosis is an important stage in the cell cycle when the duplicated chromosomes are segregated to the daughter cells. (virginia.edu)
  • To gain insight into these mechanisms, we studied the behavior of cells entering mitosis with damaged chromosomes. (ca.gov)
  • The CPC includes, as well as Aurora B, three regulatory subunits: the inner centromeric protein, Survivin, and Borealin/Dasra W. Beginning in prophase, the CPC localizes to condensing chromosomes and steadily concentrates at the internal centromere where one function would be to correct poor Infectious causes of cancer spindle kinetochore devices. (smadpathway.com)
  • Conversely, the deubiquitinating enzyme hFAM was required for the disassociation of Survivin and Aurora B from anaphase chromosomes. (smadpathway.com)
  • Remarkably, a current study contradicts these findings, indicating that p97 is needed for the disassociation of Aurora B from chromosomes, which will be in turn a requisite for nuclear envelope reformation at the end of mitosis. (smadpathway.com)
Inhibition5
  • The inhibition of Aurora kinases in order to arrest mitosis and subsequently inhibit tumor growth via apoptosis of proliferating cells has generated significant discussion within the literature. (rcsb.org)
  • Inhibition of NEK6 can lead to the termination of mitosis, chromatin spindle defects, and abnormal chromosomal differentiation [ 5 - 7 ]. (hindawi.com)
  • Acute inhibition or degradation of ATR in mitosis induces whole-chromosome missegregation. (umn.edu)
  • Analysis of SNS-314 treated tumors confirms that the anti-tumor activity is consistent with Aurora kinase inhibition. (cogershop.com)
  • Combined Aurora kinase and EGFR targeting using a monoclonal anti-EGFR antibody was more effective compared to single EGFR and Aurora kinase inhibition. (oncotarget.com)
Mitotic spindle7
  • During mitosis, a mitotic spindle is assembled by using microtubules to tether together the mother centrosome to its daughter. (wikipedia.org)
  • Aurora A is critical for proper formation of mitotic spindle. (wikipedia.org)
  • Furthermore, Aurora A is necessary for the proper separation of the centrosomes after the mitotic spindle has been formed. (wikipedia.org)
  • Without Aurora A, the mitotic spindle, depending on the organism, will either never separate or will begin to separate only to collapse back onto itself. (wikipedia.org)
  • An aurora kinase that localizes to the CENTROSOME during MITOSIS and is involved in centrosome regulation and formation of the MITOTIC SPINDLE. (lookformedical.com)
  • Aurora-B, which is a member of the Chromosome Passenger Complex (CPC), is a key mitotic kinase and plays an important role in ensuring high fidelity mitosis by phosphorylating numerous substrates in the mitotic spindle. (virginia.edu)
  • While Aurora A localizes to mitotic centrosomes and is required for centrosome growth and the development of a functional bipolar mitotic spindle, Aurora W is the catalytic core of the highly conserved genetic individual complex. (smadpathway.com)
Apoptosis2
Centrosome7
Kinases play1
Microtubules4
Poles1
  • A deconvolved wide-field fluorescence microscope image of a human HeLa cancer cell undergoing aberrant mitosis - the spindle has three poles. (mitosispictures.com)
Phosphorylation6
Pathway4
  • Figure 2: Progression through mitosis underlies inflammatory pathway activation. (nature.com)
  • Thus, a mitosis-specific and R loop-driven ATR pathway acts at centromeres to promote faithful chromosome segregation, revealing functions of R loops and ATR in suppressing chromosome instability. (umn.edu)
  • His DNA methylation research includes themes of Aurora kinase, Methylation, EZH2 and Notch signaling pathway. (research.com)
  • 3. Investigating the input upstream of Aurora B which involves a non-canonical Caspase-7 signalling pathway resulting in the proteolytic cleavage of PKCε in mitosis. (mun.ca)
Chromosome segregation1
Proteins2
  • The Myc protein and proteins that participate in mitosis represent attractive targets for cancer therapy. (nih.gov)
  • These include Rho proteins, Aurora B, and Polo proteins. (microscopemaster.com)
Induces1
  • Infection induces an increase in the number of hemocytes, and tubulin and nuclear staining showed that this increase is primarily due to mitosis and, more specifically, autonomous cell division, by circulating granulocytes. (biomedcentral.com)
Small-molecule2
  • These findings reveal a potential mechanistic link between Aurora A activation and changes in the intracellular redox state during mitosis and provide insights into how novel small-molecule inhibitors may be developed to target specific subpopulations of Aurora A. (cornell.edu)
  • SNS-314 is a potent small-molecule inhibitor of Aurora kinase that is being developed as a novel anti-cancer therapeutic agent. (cogershop.com)
NIMA1
  • NEK6 belongs to the never in mitosis A (NIMA)-related kinases family and is a mitogen/threonine kinase with 313 amino acids. (hindawi.com)
Molecular1
  • As a molecular mechanism, we demonstrate that PTPRD interacts with aurora kinase A (AURKA), an oncogenic protein that is over-expressed in multiple forms of cancer, including neuroblastoma. (biomedcentral.com)
Anaphase4
  • While this is the last event of cell division, it starts early on during the anaphase stage of mitosis in most eukaryotic cells . (microscopemaster.com)
  • In vertebrates, this drop is mediated partly by Aurora B ubiquitination via the anaphase marketing complex, and subsequent destruction by the proteasome. (smadpathway.com)
  • 1. Defining a mechanism by which the key mitotic kinase, Aurora B orchestrates safe passage through the metaphase-anaphase transition in the presence of mitotic catenation. (mun.ca)
  • Roumina Rouhani Ardeshiri, MSc Candidate Dr. Leitges' Laboratory "The Aurora B specificity switch is required to protect from nondisjunction at the metaphase/anaphase transition" Objectives: 1. (mun.ca)
Localizes1
  • In mitosis, ATR localizes to centromeres through Aurora A-regulated association with centromere protein F (CENP-F), allowing ATR to engage replication protein A (RPA)-coated centromeric R loops. (umn.edu)
Drosophila1
RNAi1
  • Intriguingly, abolishment of Aurora A through RNAi interference results in different mutant phenotypes in different organisms and cell types. (wikipedia.org)
Regulation3
  • Aurora kinase C is a chromosomal passenger protein that interacts with aurora kinase B in the regulation of MITOSIS. (lookformedical.com)
  • An aurora kinase that is a component of the chromosomal passenger protein complex and is involved in the regulation of MITOSIS. (lookformedical.com)
  • The Cdc48/p97 AAA ATPase has been linked by recent reports with the regulation of Aurora B and the chromosomal passenger complex. (smadpathway.com)
Segregation1
  • The acentric chromatid poleward movement is mediated through DNA tethers decorated with BubR1, Polo, INCENP, and Aurora-B. Reduced BubR1 or Polo function results in abnormal segregation of acentric chromatids, a decrease in acentric chromosome tethering, and a great reduction in adult survival. (ca.gov)
Inhibitor of aurora1
AURKA1
Progression2
  • Here we show that cell cycle progression through mitosis following double-stranded DNA breaks leads to the formation of micronuclei, which precede activation of inflammatory signalling and are a repository for the pattern-recognition receptor cyclic GMP-AMP synthase (cGAS). (nature.com)
  • Inhibiting progression through mitosis or loss of pattern recognition by stimulator of interferon genes (STING)-cGAS impaired interferon signalling. (nature.com)
Kinase domain2
  • In this study, x-ray crystal structures of the Aurora A kinase domain delineate redox-sensitive cysteine residues that, upon covalent modification, can allosterically regulate kinase activity and oligomerization state. (cornell.edu)
  • We further showed that covalent disulfide adducts of this residue promote autophosphorylation of the Aurora A kinase domain. (cornell.edu)
Regulates1
  • Apart from regulating kinetochore-microtubule attachment Aurora-B also regulates the assembly of the outer kinetochore during mitosis. (virginia.edu)
Inhibitors1
  • We report a novel class of Aurora kinase inhibitors based upon a phthalazinone pyrazole scaffold. (rcsb.org)
Gene2
  • The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene. (lookformedical.com)
  • William C. Reinhold combines topics linked to Mitosis with his work on Gene. (research.com)
Metaphase2
  • In the absence of Aurora A mad2, a protein that normally dissipates once a proper kinetochore-microtubule connection is made, remains present even into metaphase. (wikipedia.org)
  • Hence, this study concluded that p97/Ufd1/Npl4 is just a positive regulator of the CPC, as it is necessary for the localization of Aurora and Survivin W to metaphase centromeres. (smadpathway.com)
Cell8
  • Our previously reported finding that protein cysteine oxidation is increased during mitosis relative to other cell cycle phases suggests that redox modifications could play prominent roles in regulating mitotic processes. (cornell.edu)
  • It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. (wikipedia.org)
  • Aurora A is activated by one or more phosphorylations and its activity peaks during the G2 phase to M phase transition in the cell cycle. (wikipedia.org)
  • Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-Aurora A (Thr288) in a single step. (cellsignal.com)
  • Methods: Previously we observed that aurora kinase (AURK) binds IκBα in the cell. (researchsquare.com)
  • Aurora A overexpression in many malignant tumor types suggests that it may be directly involved in NEOPLASTIC CELL TRANSFORMATION. (lookformedical.com)
  • However, overexpression of aurora-A in particular cell lines such as NIH3T3 is sufficient to induce growth on soft agar. (lookformedical.com)
  • During the early stages of mitosis, these rings narrow to create the place of cell division. (microscopemaster.com)
INCENP1
  • Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections. (pasteur.fr)
HeLa1
  • The relationship between lysate protein concentration from paclitaxel-treated and hydroxyurea-treated HeLa cells and the absorbance at 450 nm using the PathScan ® RP Phospho-Aurora A (Thr288) Sandwich ELISA Kit #41333 is shown in the upper figure. (cellsignal.com)
Microtubule1
  • Aurora-B activity at the kinetochore changes in response to the kinetochore-microtubule attachment status and this change is important for proper mitosis. (virginia.edu)
Complex1
Cells2
  • We showed in both egg extracts and mammalian cells that a conserved cysteine residue within the Aurora A activation loop is crucial for Aurora A activation by autophosphorylation. (cornell.edu)
  • Here we report that a circumscribed exposure to the aurora kinase inhibitor, VX-680, selectively kills cells that overexpress Myc. (nih.gov)
Promotes1
Understood1
  • CPAP is required for centriole elongation during S/G2 phase, but the role of CPAP in mitosis is incompletely understood. (sinica.edu.tw)
Selective1
  • The development of the phthalazinone template resulted in a potent Aurora-A selective series of compounds (typically >1000-fold selectivity over Aurora-B) that display good pharmacological profiles with significantly improved oral bioavailability compared to the well studied Aurora inhibitor VX-680. (rcsb.org)
Stages1
  • statistically significant higher imply SUVmax, quantity of mitosis and advanced N stages compared to adenocarcinoma. (biomasswars.com)
Role1
Centrosomes3
  • In the case of the former, it has been suggested that Aurora A cooperates with the kinase Nek2 in Xenopus to dissolve the structure tethering the cell's centrosomes together. (wikipedia.org)
  • Therefore, without proper expression of Aurora A, the cell's centrosomes are never able to separate. (wikipedia.org)
  • For example, deletion of Aurora A in C. elegans results in an initial separation of the cell's centrosomes followed by an immediate collapse of the asters. (wikipedia.org)
Centromeres1
  • HDAC8 inhibitor Ufd1 was proved to be required for Survivin ubiquitination, and for the localization of Aurora and Survivin W to centromeres. (smadpathway.com)
Crucial1
  • Along with Cdk1/cyclin N, members of the Aurora/Ipl1 kinase family are also crucial regulators of mitosis. (smadpathway.com)
Passenger1
  • Although there is evidence to suggest that Aurora C might be a chromosomal passenger protein, the cellular function of it is less clear. (wikipedia.org)