• Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
  • Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
  • Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
  • Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
  • MicroRNA-543 promotes cell invasion and impedes apoptosis in pituitary adenoma via activating the Wnt/β-catenin pathway by negative regulation of Smad7. (cancerindex.org)
  • HP75 cells treated with overexpressed miR-543 exhibited increased cell proliferation, migration and invasion, while decreased cell apoptosis as well as expression of Cleaved caspase-3 and Cleaved caspase-8 were observed. (cancerindex.org)
  • These paclitaxel resistant ovarian cells demonstrate: (1) Increased IC 50 for paclitaxel and docetaxel (10 to 75-fold) and cross-resistance to anthracyclines (2) Reduced cell apoptosis in the presence of paclitaxel (3) Gene depletion involving mitotic regulators BUB1 mitotic checkpoint serine/threonine kinase, cyclin BI (CCNB1), centromere protein E (CENPE), and centromere protein F (CENPF), and (4) Functional data validating gene depletion among mitotic regulators. (biomedcentral.com)
  • Cytotoxicity, antiproliferative and apoptosis-inducing capacity of ACNVs against hepatoma carcinoma cell were assessed. (dovepress.com)
  • ACNVs were found to possess specific antitumor cell proliferation activity associated with an apoptosis-inducing pathway. (dovepress.com)
  • Hep G2 cells, transiently transfected with a luciferase reporter containing 1.67 kb of the human tTG promoter, showed an increase in reporter activity after addition of TNF-alpha. (nih.gov)
  • The A549 human lung adenocarcinoma and CL1-0 lung adenocarcinoma cell lines were cultured in Dulbecco′s modified eagle medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic antimycotic. (hindawi.com)
  • All cell lines were cultured in Dulbecco's modified eagles' medium (DMEM) supplemented with 2 mM glutamine and 10% heat inactivated fetal bovine serum (FBS) from Life Technologies (Carlsbad, CA) and maintained at 37°C in a 5% CO 2 atmosphere. (biomedcentral.com)
  • We have generated model systems to explore drug resistance in ovarian cancer, which have revealed a key pathway related to the spindle assembly checkpoint underlying paclitaxel resistance in ovarian cell lines. (biomedcentral.com)
  • Cells were exposed to an incremental dose escalation of paclitaxel (2 nM) for approximately 2 passages, up to a final concentration of 25 nM, once paclitaxel resistance was achieved. (biomedcentral.com)
  • ACNVs could be internalized into tumor cells mainly via phagocytosis, but they were quickly cleared once entering the blood. (dovepress.com)
  • Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
  • Lastly, through gene expression profiling we have demonstrated disruption of the spindle assembly checkpoint in the paclitaxel resistant cell lines, indicating a potential therapeutic pathway. (biomedcentral.com)
  • One of several postulated roles for tissue transglutaminase (tTG) is the stabilization and assembly of extracellular matrix via peptide cross-linking. (nih.gov)
  • Extracellular vesicles (EVs) are a group of cell-derived membranous structures, which play an important role in intercellular communication and functional transduction. (dovepress.com)
  • 1 , 2 In recent years, mammalian cell-derived exosomes (a type of extracellular vesicle) have been widely used in the field of biomedicine, including diagnosis, regulation of disease progression and drug delivery. (dovepress.com)
  • Hld-loaded wtMVs were cytotoxic to macrophage RAW264.7 cells and could stimulate the production of inflammatory factor IL-6 in vivo. (bvsalud.org)
  • In vivo antitumor activity and toxicity were evaluated in Hep G2 cell xenograft model. (dovepress.com)
  • Notably, terpinen-4-ol was able to increase p53 levels in A549 and CL1-0 cells. (hindawi.com)
  • Therefore, in this study, the anticancer effects of terpinen-4-ol were evaluated on two NSCLC cell lines, namely, A549 and CL1-0 human lung adenocarcinoma cells. (hindawi.com)
  • Chronic hepatitis B virus (HBV) and hepatitis D virus (HDV) infections are major causes of liver disease and hepatocellular carcinoma worldwide. (mdpi.com)
  • Human ovarian cancer cell lines TOV21G (representing clear cell ovarian carcinoma) and TOV112D (representing endometrioid adenocarcinoma) were purchased from American Type Culture Collection (Manassas, VA). The human ovarian epithelial-serous cell line COV504 was purchased from Sigma Aldrich (St. Louis, MO). (biomedcentral.com)
  • A hemolysis assay was performed with rabbit red blood cells. (bvsalud.org)
  • To further study the role of tTG in liver disease, we initiated investigations into the effect of a proinflammatory mediator, tumor necrosis factor (TNF)-alpha, on tTG activity in cultured liver cells. (nih.gov)
  • Treatment of human Hep G2 cells with 1 ng/ml TNF-alpha increased [14C]putrescine cross-linking to cellular proteins. (nih.gov)
  • In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
  • In addition, there is no report on the antitumor effects of terpinen-4-ol against human nonsmall cell lung cancer cells. (hindawi.com)
  • This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
  • We have generated paclitaxel resistant ovarian cell lines from their corresponding native cell lines to determine driver mechanisms of drug resistance using gene expression arrays. (biomedcentral.com)
  • These pre-clinical models were used to observe cytotoxicity, cell cycle modulation and changes in gene expression to examine the mechanisms driving drug resistance. (biomedcentral.com)
  • In paper IV, we show that allele substitution at rs2289669 and rs8065082 does not affect SLC47A1 expression or splicing in HepG2 cells. (lu.se)
  • Among lung cancers, nonsmall cell lung carcinomas (NSCLC) account for approximately 80% of lung cancer cases [ 1 ]. (hindawi.com)
  • Recommendations are specific for various patient groups (eg, treatment naïve, treatment experienced, coinfection with hepatitis C). (medscape.com)
  • While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. (mdpi.com)
  • The identity of each cell line was validated prior to use by short tandem repeat genotyping at The Centre for Applied Genomics at The Hospital for Sick Children (Toronto, ON). (biomedcentral.com)
  • Exosomes are a type of membrane vesicles secreted by living cells. (dovepress.com)
  • We also show that MTIF3 regulates mitochondrial respiration and endogenous fatty acid oxidation in adipocytes, probably as a consequence of MTIF3 affecting mitochondrial oxidative phosphorylation complex assembly. (lu.se)
  • Previous evidence points to numerous components of the spindle assembly checkpoint and mitotic regulation playing a major role in several cancers [ 11 , 12 ]. (biomedcentral.com)
  • Cell Proliferation, Differentiation and Angiogenesis IL-6-STAT3 Pathway As a gender-dependent risk factor for HCC explaining why females are less prone to liver cancer than males, [ 12 , 23 ] IL-6 is a HCC bio-marker and an ideal molecular target to be aimed at. (medscape.com)
  • sample_type A C177537 GDC Value Terminology C116938 CDK4/6 Inhibition Inhibition of cyclin-dependent kinases 4 and 6 pathway activity to prevent proliferation of cancer cells and tumor growth. (nih.gov)
  • This prevents tumor cell proliferation in cancer cells overexpressing ROR1. (nih.gov)
  • sCLU, a heterodimeric disulfide-linked glycoprotein overexpressed by various types of cancer cells, contributes to proliferation and survival of cancer cells, and stimulates tumor cell EMT. (nih.gov)
  • TPX2-p53-GLIPR1 regulatory circuitry in cell proliferation, invasion, and tumor growth of bladder cancer. (cancerindex.org)
  • This study aims to explore the interplay between TPX2, p53, and GLIPR1 and its correlation with cell proliferation, invasion, and tumor growth in bladder cancer. (cancerindex.org)
  • This study identified a novel TPX2-p53-GLIPR1 regulatory circuitry which modulated cell proliferation, migration, invasion, and tumorigenicity of bladder cancer. (cancerindex.org)
  • The mechanisms how alcohol and viral hepatitis together disorder, can have devastating effects and account for 5.9 accelerate liver disease have been researched extensively over percent of deaths and 5.1 percent of the global burden of the last several decades. (nih.gov)
  • It is becoming clear that alcohol disease and injury, thereby also imposing a significant social exposure, infection with hepatitis viruses, and the host's and economic burden on society (World Health Organization defense mechanisms against these offenders combine to 2015). (nih.gov)
  • The body's immune response may also be keeping these cells in check but cancer cells can develop mechanisms to overcome the immune response. (nih.gov)
  • [ 13 ] Key cellular functions are inhibited by statins selectively in various karyotypically abnormal cell types (including colorectal and ovarian cancer cells and human embryonic stem cells, which possess neoplastic-like properties) and this is mediated via a suppression of the stemness pathway. (medscape.com)
  • Paul Sammak: There are neuronal progenitor cells that do reproduce in parts of the brain. (nih.gov)
  • [ 5 ] HCC displays perturbed cholesterol metabolism both within mitochondria and in cell membranes. (medscape.com)
  • [ 19 ] Increased cholesterol levels in mitochondria from either rat or human HCC cells contribute to chemotherapy resistance and cholesterol depletion by inhibition of hydroxymethylglutaryl-CoA reductase enhances sensitivity to chemotherapy. (medscape.com)
  • Tumor cell lines were evaluated using the following tests: trypan blue exclusion test, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], flow cytometry, cytoskeleton organization analysis, cell cycle, mitochondria membrane depolarization, clonogenicity test, DNA fragmentation test and differential protein expression by HPLC-Chip/MS analysis. (biomedcentral.com)
  • Hepatitis include gender, co-exposure to other drugs, genetic factors that either favor the development of addiction or affect alcohol-metabolizing enzymes, immunological factors, nutritional status, and infection with viruses targeting the 1 in 3 children starts drinking by the Role of Lipid Rafts liver (i.e., hepatotropic viruses). (nih.gov)
  • Cell Growth/Survival Pathways The proto-oncogene myc (c-myc) codes for a nuclear protein, which controls nucleic acid metabolism and mediates the cellular response to growth factors. (medscape.com)
  • Compound 13 was a potent inhibitor of the human U87MG glioblastoma cells at nanomolar concentrations, being nearly one order of magnitude superior to previously reported arylthioindoles. (unicatt.it)
  • Compounds 13 and 19 inhibited a panel of cancer cells and the NCI/ADR-RES multidrug resistant cell line at low nanomolar concentrations. (unicatt.it)
  • During disease development, cancer cells acquire multiple key biological capabilities conferring them a competitive survival advantage and culminating in invasion and metastasis. (medscape.com)
  • The 6- and 7-heterocyclyl-1H-indoles showed potent inhibition of tubulin polymerization, binding of colchicine to tubulin and growth of MCF-7 cancer cells. (unicatt.it)
  • The protein has proapoptotic activities in prostate and bladder cancer cells. (cancerindex.org)
  • The expressions of TPX2 and p53 in non-muscle-invasive bladder cancer cells (KK47 and RT4) were lower than those in muscle-invasive bladder cancer cells (T24, 5637, and UM-UC-3), while GLIPR1 showed the converse expression pattern. (cancerindex.org)
  • Interestingly, in T24 cells with mutated p53, p53 silence suppressed bladder cancer growth. (cancerindex.org)
  • [ 24 ] IL-6 activates the transcription factor STAT3 (signal transducer and activator of transcription 3), an acute-phase response factor, which is next phosphorylated by the receptor associated kinases, and then forms homo- or hetero-dimers that translocate to the cell nucleus where it acts as a transcription activator. (medscape.com)
  • Marriotts Ridge, Maryland: What other eukaryotic cells besides red blood cells doesn't have a nucleus? (nih.gov)
  • The only two types of human cells that don't have a nucleus are red blood cells and blood platelets. (nih.gov)
  • To explore the cellular entry mechanism of TFV, HepG2 cells were treated with drugs that inhibit the main endocytic pathways. (bvsalud.org)
  • In contrast, TFV entry was not influenced by chlorpromazine or overexpression of a dominant-negative form of Esp15, which inhibit the assembly of clathrin -coated pits. (bvsalud.org)
  • As an inhibitor of HepG2 cells (IC50 = 20 nM), 13 was 4-fold superior to 19. (unicatt.it)
  • Entry of tiger frog virus (an Iridovirus) into HepG2 cells via a pH-dependent, atypical, caveola-mediated endocytosis pathway. (bvsalud.org)
  • This inhibits both the sCLU-mediated signal transduction pathways and epithelial-to-mesenchymal transition (EMT), which leads to the inhibition of tumor cell migration and invasion. (nih.gov)
  • Once neurons are terminally differentiated and interconnect to neighbors, they change their genetic expression pattern to suppress cell division. (nih.gov)
  • HN - 2010 FX - Polysomnography MH - Acute Chest Syndrome UI - D056586 MN - C8.381.74 MN - C8.618.09 MN - C15.378.71.141.150.150.219 MN - C15.378.420.155.219 MN - C16.320.70.150.219 MN - C16.320.365.155.219 MS - Respiratory syndrome characterized by the appearance of a new pulmonary infiltrate on chest x-ray, accompanied by symptoms of fever, cough, chest pain, tachypnea, or DYSPNEA, often seen in patients with SICKLE CELL ANEMIA. (nih.gov)
  • Cells have an important role in maintaining the DNA, making accurate copies of the DNA when cells divide, and allowing the appropriate expression of genes from the DNA. (nih.gov)
  • 1993). Co-occurring viral hepatitis and role in the lifecycles of hepatitis viruses. (nih.gov)
  • This atypical caveola -mediated endocytosis is different from the clathrin -mediated endocytosis of frog virus 3 (FV3) by BHK cells , which has been recognized as a model for iridoviruses . (bvsalud.org)
  • HBV) and hepatitis C virus (HCV), are responsible for most cases of chronic hepatitis in the United States. (nih.gov)
  • Additionally, there is belief that the number of cell types in the brain are far more varied than previously thought. (nih.gov)
  • Usually brain cells are divided along metabolic and neurotransmitter releasing properties, but there is increasing belief that there are brain cell types that can be defined exclusively by their morphological (structural) properties, and that these properties may help reveal unique functions helpful for brain function. (nih.gov)
  • Coppell Middle School North, Texas: Why do plants and animals need to have different types of cells? (nih.gov)
  • These cells could receive signals from hormones or growth factors that then stimulate the cells to divide. (nih.gov)
  • Inherent or acquired resistance can occur simultaneously to multiple drugs in the majority of tumor cells [ 1 - 4 ]. (biomedcentral.com)
  • We assessed multiple in vitro biological activities of an ethyl acetate soluble plant fraction over several tumor cell lines. (biomedcentral.com)