• An enzyme-linked immunosorbent assay (ELISA)-based secondary screen is used to identify the most promising scFvs for additional characterization. (jove.com)
  • This antibody showed binding activity to both FLAG peptide and FLAG peptide tagged protein in enzyme-linked immunosorbent assay and Western blot. (kjom.org)
  • The recombinant human FGF18 was expressed as N-terminal 6xHis-tagged fusion. (cusabio.com)
  • For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of anti·His antibodies. Detection of 6xHis-tagged proteins with Anti·His Antibodies (Tetra·His Antibody in the center). A: DHFR; B: DHFR; C: thioredoxin; D: TNF-α; E: TFIIA γ ; F: chaperonin; G DNA polymerase; H: TFIIAαß; for tag location and sequences detected see table 'Proteins detected with QIA express Anti·His Antibodies'. Indicated amounts of pure 6xHis-tagged protein were applied to a nitrocellulose membrane, and detection was carried out with the Anti·His primary antibody indicated diluted 1/2000, followed by chromogenic detection with AP-conjugated rabbit anti-mouse IgG and NBT/BCIP. "> "Sensitivity of QIA express Anti·His Antibodies" ). (qiagen.com)
  • The QIA express System provides materials for the expression, purification, detection and assay of 6xHis-tagged proteins. (qiagen.com)
  • The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
  • He also noted concern regarding delays in referring specimens to the laboratory for testing, and emphasized the need for improvement in timeliness of reporting and tracking of the viruses referred for genomic sequencing. (who.int)
  • He thanked the regional and global specialized laboratories for their efforts to provide timely results of the genomic sequencing of wild polioviruses, and for their help in planning and monitoring polio eradication activities in the Region. (who.int)
  • Finally, the antigenicity of these PUF proteins was evaluated by determining levels of specific antibodies in sera from experimentally infected hamsters. (biomedcentral.com)
  • Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA-free and Penta·His Antibody, BSA-free are used to detect recombinant protein carrying His tags. (qiagen.com)
  • Anti·His Antibodies enable identification of positive expression clones by colony blotting (see figure Identification of positive expression clones by colony blotting. . "> "Identification of positive expression clones by colony blotting" ) and detection of His-tagged thioredoxin (see figure His-tagged thioredoxin detected by Penta·His Antibody in yeast cells. . "> "His-tagged thioredoxin detected by Penta·His Antibody in yeast cells" ). (qiagen.com)
  • For detection, QIA express mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. (qiagen.com)
  • Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. (jove.com)
  • These methods are powerful and effective for identifying antibodies that bind to targets, yet they depend on the secretory pathway to transport proteins that will be displayed 14-16 . (jove.com)
  • View side-by-side comparison of DYKDDDDK tag antibodies from other vendors to find the one that best suits your research needs. (ptglab.com)
  • Accordingly, immunohistochemistry with specific antibodies shows that HMGCS2 is expressed mainly in differentiated cells of human colonic epithelium. (aacrjournals.org)
  • Capsulin was translated in a rabbit reticulocyte lysate in the presence and absence of the widely expressed bHLH protein E12 and performed gel mobility shift assays with several E-box sequences as probes to test the protein's DNA binding activity. (wikipedia.org)
  • The interactions between AmtR and the promoter regions of the three operons were confirmed by electrophoretic mobility shift assays (EMSAs). (frontiersin.org)
  • Here, we review the arrival of genetic screens in malaria parasites to analyse parasite gene function at a genome-scale and their impact on understanding parasite biology. (portlandpress.com)
  • First, Tim Graubert and colleagues describe the whole-genome sequencing of an MDS-derived secondary AML tumor and a matched normal (skin) sample. (massgenomics.org)
  • The PEDV has an approximately 28-kilobase pair genome, including seven open reading frames, and encodes both non-structural and structural proteins [ 4 ]. (biomedcentral.com)
  • Genome-wide RNA expression studies in ES cells expressing a V5- and GFP-tagged form of CTCFL show that genes that are downregulated in CTCFL-deficient testis are upregulated in ES cells. (unican.es)
  • Furthermore, genome-wide DNA-binding analysis shows that CTCFL binds a consensus sequence that is very similar to that of CTCF. (unican.es)
  • Critical to sub-classify SRBCTs, Khan et al .6 used to measure genome-wide gene expres- .com decisions in cancer therapeutics are to microarrays to monitor the expression sion for a variety of biological samples. (lu.se)
  • Here, through an in-depth phylogenetic clustering analysis of upwards of 200,000 whole-genome sequences, we reveal the presence of previously unreported and hitherto unidentified mutations and recombination breakpoints in Variants of Concern (VOC) and Variants of Interest (VOI) from Brazil, India (Beta, Eta and Kappa) and the USA (Beta, Eta and Lambda). (cdc.gov)
  • Genome sequencing on the Oxford Nanopore platform was performed using the ARTIC approaches for library preparation and bioinformatic analysis. (cdc.gov)
  • The Tat pathway ensures that only soluble, well-folded proteins are transported out of the cytoplasm and displayed on the inner membrane, thereby eliminating poorly folded scFvs prior to interrogation for antigen-binding. (jove.com)
  • However, the amino acid sequences involved in antigen binding was unknown. (kjom.org)
  • Recently, the amino acid sequences of the variable domain, which is involved in the antigen binding, has been determined through X-ray crystallography and Mass spectroscopy. (kjom.org)
  • ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. (transcriptionfactor.org)
  • Results showed that in humans and mice, Pod-1 was most highly expressed in the kidney, lung and heart, with selective expression at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine and pancreas of developing mouse embryos. (wikipedia.org)
  • Its restricted expression pattern and DNA binding activity identified Capsulin as a regulator of gene expression in specific subtypes of visceral mesodermal cells involved in organogenesis and in precursor cells that contribute to the pericardium, coronary arteries and regions of the heart. (wikipedia.org)
  • Real-time polymerase chain reaction was used to detect the dynamic mRNA expression of this gene in the spleen, thymus, bursa of Fabricius, and trachea of NDV-infected chickens. (geneticsmr.com)
  • The expression of this gene at the protein level was further confirmed in the spleen, thymus, and bursa of Fabricius of NDV-infected chickens using Western blot analysis. (geneticsmr.com)
  • Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source. (frontiersin.org)
  • DAVID was used to perform the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis for the different expression genes (DEGs). (medscimonit.com)
  • This study provides a differential gene expression profile between TMJ fibrous and bony ankylosis. (medscimonit.com)
  • In many cases, the disease gene encodes a component of phototransduction or allied processes with expression confined to the retina, for example the rod visual pigment (reviewed in [ 1 ]), or peripherin-RDS [ 2 ], but not all RP genes show such a restricted pattern of expression. (molvis.org)
  • Using a recombinant endonuclease-deficient Cas9-based gene promoter pulldown approach coupled with mass spectrometry, we found that MCUb is upregulated in the T2D heart due to loss of glucose homeostasis regulator nuclear receptor corepressor 2 repression, and chromatin immunoprecipitation assays identified peroxisome proliferator-activated receptor α as a mediator of MCUb gene expression in T2D cardiomyocytes. (diabetesjournals.org)
  • investigated the fungus at the level of gene expression, and performed proteome-level studies ( Yajima and Kav, 2006 ). (frontiersin.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. (caslab.com)
  • There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. (nhbs.com)
  • Using potato virus X (PVX) based transient expression assays, we showed that one of the candidate effectors, i. e. contig 8 that encodes a cerato-platanin (CP) domain containing protein, unlike CP proteins from other fungal pathogens was unable to elicit a hypersensitive response (HR). (biomedcentral.com)
  • By screening an L. infantum expression library with sera from human VL patients or infected hamsters, they identified 43 genes encoding B-cell antigens, and, remarkably, 44% of those antigens contained tandem repeats in their structure. (biomedcentral.com)
  • Representative σ 66 -dependent gene transcription was repressed in the absence of rsbV1 or upon increased expression of RsbW, and increased upon elevated expression of RsbV1. (plos.org)
  • The primary data for 1-step growth, 2-step growth and gene expression summarized in Figs 4 and 5 are available in the S1 Dataset in the supplementary information. (plos.org)
  • The expression of 11 angiogenesis-associated biological factors and 11 cytokines secreted by EnSCs and 17 adhesion molecules expressed on EnSCs were determined by protein array assays respectively. (endometriosistreatmentreport.com)
  • Two PEDV spike (S) glycoprotein-based indirect enzyme-linked immunosorbent assays (ELISAs) were developed using G2b PEDV-Pintung 52 (PEDV-PT) trimeric full-length S and truncated S 1-501 proteins derived from the human embryonic kidney (HEK)-293 cell expression system. (biomedcentral.com)
  • We found that knockdown of Bonus in early oogenesis results in severe defects in ovarian development and in ectopic expression of genes that are normally repressed in the germline, demonstrating its essential function in the ovary. (elifesciences.org)
  • Together, our work identified Bonus as a regulator of tissue-specific gene expression and revealed the importance of SUMOylation as a regulator of complex formation in the context of transcriptional repression. (elifesciences.org)
  • Epigenetic regulation of gene expression is an essential mechanism that guides cell differentiation during development. (elifesciences.org)
  • Comparison of mRNA levels of P. falciparum gametocytes before and 30 min following activation using suppression subtractive hybridization (SSH) identified 126 genes, which changed in expression during gametogenesis. (biomedcentral.com)
  • Among these, 17.5% had putative functions in signaling, 14.3% were assigned to cell cycle and gene expression, 8.7% were linked to the cytoskeleton or inner membrane complex, 7.9% were involved in proteostasis and 6.4% in metabolism, 12.7% were cell surface-associated proteins, 11.9% were assigned to other functions, and 20.6% represented genes of unknown function. (biomedcentral.com)
  • Of these, 22 genes were expressed in gametocytes, and for 15 genes transcript expression in gametocytes was increased compared to asexual blood stage parasites. (biomedcentral.com)
  • For selected genes, a regulated expression during gametogenesis was confirmed on the protein level, using quantitative confocal microscopy. (biomedcentral.com)
  • The obtained transcriptome data demonstrate the regulations of gene expression immediately following malaria parasite transmission to the mosquito. (biomedcentral.com)
  • Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)
  • Depending on the newly determined variable heavy and light chain amino acid sequences of the MassM2 antibody, corresponding genes were designed, synthesized, and cloned into the pUC119 expression vector and then expressed in Escherichia coli as a recombinant MassM2 single chain variable fragment (ScFv) antibody. (kjom.org)
  • The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. (ptglab.com)
  • In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
  • Here, we used a chromatin immunoprecipitation assay to study the molecular mechanism responsible for this expression pattern. (aacrjournals.org)
  • HMGCS2 protein expression is down-regulated preferentially in moderately and poorly differentiated carcinomas. (aacrjournals.org)
  • The factors responsible for the postnatal and starvation-associated induction of HMGCS2 gene expression have been identified and shown to involve changes in circulating hormone and fatty acid levels. (aacrjournals.org)
  • Here we demonstrate that, during brain maturation, the potential of the intrinsic apoptotic pathway is progressively reduced and that such repression is associated with downregulation of apoptotic protease-activating factor-1 (Apaf-1) and caspase-3 gene expression. (jneurosci.org)
  • Injury-induced cytochrome c -specific cleavage of caspase-9 followed by activation of caspase-3 in mature brain correlated with marked increases in Apaf-1 and caspase-3 mRNA and protein expression. (jneurosci.org)
  • These results suggest that differential expression of Apaf-1 and caspase-3 genes may underlie regulation of apoptotic susceptibility during brain development, as well as after acute injury to mature brain, through the intrinsic pathway of caspase activation. (jneurosci.org)
  • In conjunction with cohesin, it organizes chromatin into loops, thereby regulating gene expression and epigenetic events. (unican.es)
  • CTCFL deficiency affects the expression of a number of testis-specific genes, including Gal3st1 and Prss50. (unican.es)
  • We propose that the transient expression of CTCFL in spermatogonia and preleptotene spermatocytes serves to occupy a subset of promoters and maintain the expression of male germ cell genes. (unican.es)
  • 0 gene-expression profiling to tinct subtypes of SRBCTs (Fig. 1). (lu.se)
  • Gene-expression patterns used to differentiate four type cause heart damage, and markable finding that genes cur- types of SRBCT. (lu.se)
  • Because the transcript they found was highly expressed in visceral glomerular epithelial cells (podocytes), TCF21 was initialled named Pod-1. (wikipedia.org)
  • Endocytosis is the vesicle-mediated process used by all cells to internalize extracellular macromolecules, plasma membrane lipids, and plasma membrane proteins ( Figure 1 ). (wormbook.org)
  • Splicing activity was assayed using an in vivo assay in transfected mammalian cells with rhodopsin ( RHO ) and transducin ( GNAT1 ) splicing templates. (molvis.org)
  • In the hepatocellular carcinoma microenvironment, we identified intermediate c-kit+ ILC2 population, and lin-CD127- NK-like cells that expressed markers of cytotoxicity including CCL5 and IFNG. (bvsalud.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. (caslab.com)
  • Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separations - Regis Dieckmann, Yohanne Coute, Denis Hochstrasser, Jean-Jacques Diaz and Jean-Charles Sanchez 11. (nhbs.com)
  • IL-27 is expressed by monocytes, endothelial cells and dendritic cells. (creativebiomart.net)
  • Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. (creativebiomart.net)
  • Thus, existing techniques and current knowledge have not used nor addressed the interactions of GEF-H1 with other proteins as a means by which cell proliferation can be controlled or the detection and treatment of cancerous, tumorigenic cells and tissues be developed. (justia.com)
  • They have the ability to modulate protein activity by binding to a target protein inside cells to prevent protein-protein interactions, disrupt protein-nucleic acid interactions, or prevent substrate access to enzymes 1-5 . (jove.com)
  • These cells display the characteristic markers of AAM including upregulated arginase-1 (arg-1), Ym1, FIZZ and also expressed IL-10. (biomedcentral.com)
  • HEK-293T cells and transfected HEK-293T lysates were subjected to SDS PAGE followed by western blot with 20543-1-AP (DYKDDDDK tag antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. (ptglab.com)
  • The proliferation of RCC cells was analyzed using MTT and EdU incorporation assays. (thno.org)
  • The migration of RCC cells was evaluated by wound healing and Transwell migration assays. (thno.org)
  • The large proteoglycan molecules made up of protein cores, and GAG branches are secreted by cells and constitute a significant fraction of the extracellular matrix of connective tissue. (medscape.com)
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)
  • Additionally, the 10 genes of the L. infantum PUF family were cloned and used to express the Leishmania PUFs in bacteria as recombinant proteins. (biomedcentral.com)
  • The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. (creativebiomart.net)
  • The invention also envisages a peptide consisting essentially of the sequence of SEQ ID NO. 3. (justia.com)
  • In another embodiment, a peptide consisting essentially of the sequence of SEQ ID NO. 4 is also provided. (justia.com)
  • In another embodiment, serine-67 is present in a peptide of less than 30 amino acids that comprises the sequence of SEQ ID NO. 4. (justia.com)
  • In another embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is less than 25 amino acids in length. (justia.com)
  • In a more preferred embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is 18 amino acids long. (justia.com)
  • The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
  • An anti-FLAG-M2 mouse monoclonal antibody specific to the FLAG peptide (DYKDDDDK) is widely used worldwide to purify or detect FLAG-tagged proteins. (kjom.org)
  • This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. (ptglab.com)
  • The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. (ptglab.com)
  • Gene therapy displacement of endogenous MCUb with a dominant-negative MCUb transgene (MCUb W246R/V251E ) in vivo rescued T2D cardiomyocytes from metabolic inflexibility and stimulated cardiac contractile function and adrenergic responsiveness by enhancing phospholamban phosphorylation via protein kinase A. We conclude that MCUb represents one newly discovered molecular effector at the interface of metabolism and cardiac function, and its repression improves the outcome of the chronically stressed diabetic heart. (diabetesjournals.org)
  • Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
  • The full-length cDNA sequence of a novel expressed sequence tag (GenBank accession No. HQ184338) that was differentially expressed during Newcastle disease virus (NDV) infection in chickens was cloned from the chicken spleen by a rapid amplification of cDNA ends assay. (geneticsmr.com)
  • In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
  • Deep cDNA sequencing demonstrated that both wild-type and mutant alleles were expressed . (massgenomics.org)
  • Studies conducted on PRPF31 mRNA levels in lymphoblast cell lines isolated from family members with either deletion or splice site mutations indicate that penetrance requires the co-inheritance of a low expressing wild-type (WT) allele alongside the mutant allele [ 13 , 14 ], whereas the presence of a high expressing WT allele is protective. (molvis.org)
  • In contrast, mRNA levels are now shown not to change by both Northern and RNAse protection assays. (embl.de)
  • This lack of increase contrasts with previous reports as does the mRNA length: this is 50% larger as confirmed by sequencing the 3' end. (embl.de)
  • In rats, hepatic ketogenic capacity increases rapidly during postnatal development ( 4 ) or fasting ( 5 ) when the mRNA, protein, and activity of HMGCS2 increase in liver ( 6 - 8 ). (aacrjournals.org)
  • Administration of dibutyryl-cyclic AMP to adult rats increases mRNA, protein levels, and enzymatic activity of HMGCS2 in the liver ( 6, 9 ). (aacrjournals.org)
  • Accordingly, the present invention envisions an isolated nucleic acid encoding the GEF-H1S polypeptide of SEQ ID NO:2, as well as an isolated nucleic acid comprising the sequence of SEQ ID NO:1. (justia.com)
  • Because ISH targets nucleic acid sequences, as opposed to proteins, ISH can detect both expressed and unexpressed genes, making it a versatile and less costly diagnostic tool. (technologypublisher.com)
  • These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks and hyperdynamic binding of structural chromatin proteins. (biomedcentral.com)
  • This week in Nature Genetics , two studies report recurrent mutations in splicing-related genes in blood tumors. (massgenomics.org)
  • The authors used artificial neural croarrays is to study changes in gene ex- .nature organ the tumor arose from, how big it networks (ANNs) to identify gene-ex- pression that accompany changes in is, and what it looks like after it has pression signatures associated with spe- cell physiology, such as during develop- been imbedded in wax and colored cific subtypes of tumors. (lu.se)
  • 0 blue-cell tumors (SRBCTs), which type, 41 (including 7 expressed often masquerade as each other. (lu.se)
  • The open reading frame (ORF) contained 261 bp and encoded a deduced protein of 86 amino acid residues. (geneticsmr.com)
  • RNA-binding proteins of the PUF family share a conserved domain consisting of tandemly repeated 36-40 amino acid motifs (typically eight) known as Puf repeats. (biomedcentral.com)
  • Therefore, in this study, the amino acid sequences of the heavy and light chain variable domains named as MassM2 were newly redesigned by aligning the amino acid sequences of the heavy and light chain variable domains determined by two methods mentioned above. (kjom.org)
  • HA proteins bind to sialoside receptors on the host cell surface. (cdc.gov)
  • Although a recent study ( 22 ) reported enhanced avidity of H5N6 viruses for human-type receptors, recombinant clade 2.3.4.4 highly pathogenic influenza A virus H5 proteins from virus isolates in North America show a strict avian receptor-binding preference ( 23 ). (cdc.gov)
  • Many receptors and their associated ligands cluster into clathrin-coated pits by association with clathrin adaptor proteins such as the four-subunit complex AP2. (wormbook.org)
  • Trafficking of yolk and yolk receptors also depends critically upon the activities of the endocytic Rab proteins RAB-5 , RAB-7 , and RAB-11 , known modulators of endocytosis in all eukaryotes ( Grant and Hirsh, 1999 ). (wormbook.org)
  • Fgf18 is generally known to activate the IIIc splice variants of FgfR2 and FgfR3, and both of these receptors are known to be expressed in chondrocytes of human and murine articular cartilage. (cusabio.com)
  • Fig. 1: Photochemistry of light-oxygen-voltage receptors and sequences of proteins under study. (nature.com)
  • Then, the proliferative capacity, migratory capacity and angiogenic potential of EnSCs were evaluated by conventional MTT assay, flow cytometry, wound healing assay, transwell assay, tube formation assay and chick embryo chorioallantoic membrane assay respectively. (endometriosistreatmentreport.com)
  • A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. (ptglab.com)
  • The traditional triad in the medical diagnosis of multiple myeloma may be the recognition of M proteins in the serum or urine (Bence-Jones proteins), higher than 10% plasmacytosis in the bone tissue marrow, and the current presence of osteolytic lesions by radiography. (siamtech.net)
  • To obtain a fusion protein and prepare rabbit anti-serum, the predicted ORF of this gene was expressed in Escherichia coli . (geneticsmr.com)
  • YP170-GFP, like endogenous yolk, is a cholesterol binding/transport protein related to human ApoB-100, the major protein component of serum low-density lipoprotein (LDL). (wormbook.org)
  • The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. (creativebiomart.net)
  • A real-time reverse transcription-polymerase chain (e.g., gel electrophoresis), were time-consuming, and were reaction (RT-PCR) assay was developed to rapidly detect prone to false-positive results from amplicon contamina- the severe acute respiratory syndrome-associated coron- tion. (cdc.gov)
  • PCR assay or culture isolation and proved suitable to probe hydrolysis technology (Applied Biosystems, Foster detect SARS-CoV in clinical specimens. (cdc.gov)
  • Diverse KRAB-ZFPs recognize specific DNA sequences with the majority targeting endogenous retroviruses, ensuring their repression. (elifesciences.org)
  • Based on these findings, we propose that ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2 . (aacrjournals.org)
  • Eldadah and Faden, 2000 ), we hypothesized that suppression of apoptotic capability during maturation of mammalian brain results from repression of genes involved in the caspase-3 activation pathway, and that injury-induced neuronal apoptosis in the mature brain results from reactivation of these genes. (jneurosci.org)
  • Mass spectrometry, immunoprecipitation, and immunoblotting were employed to examine protein-protein interactions. (thno.org)
  • The purity of the protein is greater than 90% assayed by SDS-PAGE. (cusabio.com)
  • These phenotypes appear to result, at least in part, from impaired interactions of the mutant β159 clamp protein with the replicative DNA polymerase, Pol III ( 38 ). (asm.org)
  • The transcriptional profile of 11 fungal genes related to pathogenicity and virulence were evaluated by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). (frontiersin.org)
  • Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
  • The DEGs were also typed into protein-protein interaction (PPI) networks to get the interaction data. (medscimonit.com)
  • Ten DEGs, including 7 hub genes from PPI analysis, were confirmed by real-time PCR. (medscimonit.com)
  • A total of 523 DEGs between EnSC-EM-EC and EnSC-Control were identified and analysed using the KEGG and Gene Ontology databases. (endometriosistreatmentreport.com)
  • Results suggested that the gene was involved in the transcriptional response of chicken to NDV infection. (geneticsmr.com)
  • The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
  • The assay revealed that HMGCS2 is a direct target of c-Myc, which represses HMGCS2 transcriptional activity. (aacrjournals.org)
  • We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane. (jove.com)
  • To better understand gene regulations during the initial phase of malaria parasite transmission, we focused on the transcriptome changes that occur within the first half hour of parasite development in the mosquito. (biomedcentral.com)
  • RS_01910 may encode a protein related to creatine transport. (frontiersin.org)
  • The ∼30 Mb genomes of the Plasmodium parasites that cause malaria each encode ∼5000 genes, but the functions of the majority remain unknown. (portlandpress.com)
  • Nearly 39% of the ESTs were predicted to encode proteins of fungal origin and among these, 162 ESTs were predicted to contain N-terminal signal peptides (SPs) in their deduced open reading frames (ORFs). (biomedcentral.com)
  • Eleven candidate effector genes were identified based on features common to characterized fungal effectors, i.e. they encode small, soluble (lack of transmembrane domain), cysteine-rich proteins with a putative SP. (biomedcentral.com)
  • Moreover, since more than half of Plasmodium genes are required for normal asexual blood-stage reproduction, and cannot be targeted using knockout methods, we discuss how CRISPR/Cas9 could be used to scale up conditional gene knockdown approaches to systematically assign function to essential genes. (portlandpress.com)
  • The secretory pathway translocates unfolded proteins from the reducing cytoplasm into the endoplasmic reticulum lumen in yeast or into the periplasm in bacteria. (jove.com)
  • Microorganisms capable of catabolizing lignin-derived ar- carbohydrate and protein components of the organic matter omatics are essentially fungi and bacteria (Bugg et al. (lu.se)
  • Pod-1 was the first tissue-restricted bHLH protein to be identified in the developing kidney and tied to regulation of morphogenetic events. (wikipedia.org)
  • The rat HMGCS2 gene contains elements that control its multihormonal regulation and tissue specificity ( 11 - 14 ). (aacrjournals.org)
  • Furthermore, this gene was found to be involved in the response of chickens to NDV infection. (geneticsmr.com)
  • For a selected subset of CEAPs and candidate effectors, semiquantitative RT-PCR showed that these transcripts were either expressed constitutively in both in vitro and in planta or induced during plant infection. (biomedcentral.com)
  • The N protein produced during the early infection stages is the most abundant protein throughout the entire viral propagation process [ 5 , 6 ]. (biomedcentral.com)
  • City, CA), has been shown to provide sensitive, specific, assay will aid in diagnosing SARS-CoV infection. (cdc.gov)
  • In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
  • The male germ cell gene regulator CTCFL is functionally different from CTCFand binds CTCF-like consensus sites in a nucleosome composition-dependent manner. (unican.es)
  • These data indicate that CTCFL is a male germ cell gene regulator. (unican.es)
  • Furthermore, the encoded protein contained two transmembrane regions without signal peptides, indicating that this protein is located in the mitochondrial membrane. (geneticsmr.com)
  • The pore-forming BCL-2 family proteins mediate mitochondrial poration to initiate apoptosis through protei-protein and protein-lipid interactions. (portlandpress.com)
  • Analysis of Membrane Proteins by Two-Dimensional Gels - Michael Fountoulakis, 15. (nhbs.com)
  • His tag is often used for affinity purification and binding assays. (caslab.com)
  • Comparison of Pod-1 with previously characterized bHLH proteins identified Pod-1 as a novel member of a subfamily of bHLH proteins with important roles in mesodermal development. (wikipedia.org)
  • In vertebrates this subfamily contains four proteins: TIF1α/TRIM24, TIF1β/TRIM28, TIF1γ/TRIM33, and TIF1δ/TRIM66, while only one protein, Bonus (Bon), is present in Drosophila , making it an attractive model to understand the conserved functions of TIF1 proteins. (elifesciences.org)
  • The present invention envisions phosphorylated forms of any one of these GEF-H1 peptides, polypeptides and proteins. (justia.com)
  • Thus, in one embodiment, peptides comprising the sequences described in SEQ ID NOs. (justia.com)
  • The production of these constructs induced the formation of high molecular aggregates with a potential impact on the efficacy and safety of the protein. (bmj.com)
  • A model is proposed where the relative levels of active antagonist (RsbV1) and switch-protein anti-sigma factor (RsbW) control the availability of σ 66 and subsequently act as a molecular 'throttle' for Chlamydia growth and development. (plos.org)
  • In order to develop T cell-based assays for the diagnosis of active TB, it is imperative to identify new host markers expressed in response to established M.tb -dependent antigens, such as those used in IGRAs (ESAT-6, CFP-10, TB7.7), or novel M.tb antigens uniquely recognized by patients with active TB or LTBI. (biomedcentral.com)
  • 12q14 (Sanfilippo syndrome): The diagnosis requires a specific lysosomal enzyme assay for glucosamine ( N -acetyl)-6-sulfatase (GNS) activity. (medscape.com)
  • TCF21 was discovered in 1998 when search for novel cell-type-specific bHLH proteins expressed in human and mouse kidneys by performing a search of the expressed sequence tag (EST) databases. (wikipedia.org)
  • The N-terminal 6xHis tag sequence was appended to the gene coding for the E.coli of the human FGF18 protein to form the recombinant DNA. (cusabio.com)
  • CRISPR/Cas9 screens, which have revolutionised human and model organism research, have not yet been implemented in malaria parasites due to the need for more complex CRISPR/Cas9 gene targeting vector libraries. (portlandpress.com)
  • Results GrB(C210A)-Fc-IT4 protein exhibited high affinity and selective cytotoxicity within the nanomolar range when tested against a panel of Fn14-positive human cancer cell lines. (bmj.com)
  • The reactivity of hamster sera against recombinant LiPUF1 and LiPUF2 was particularly prominent, and these proteins were subsequently assayed against sera from human patients. (biomedcentral.com)
  • Recombinant Human EBI3(Arg21-Lys229) and p28(Phe29-Pro243) fused with His tag at C-terminal was expressed in NS0. (creativebiomart.net)
  • Chlamydia trachomatis is an obligate intracellular human pathogen that exhibits stage-specific gene transcription throughout a biphasic developmental cycle. (plos.org)
  • This protein carries a human IgG1 Fc tag at the C-terminus. (transcriptionfactor.org)
  • AN - check the tag INFANT HN - 2008 FX - Child Nutrition FX - Infant Nutrition Physiology FX - Milk FX - Milk, Human DH - Adolescent Nutrition DI - 052508 MN - SP6.021.067 MS - Nutrition of persons 10 through 19 years of age. (bvsalud.org)
  • Structural biology has been used to elucidate their mechanisms of activation, dimerization, and interaction with membranes, and AlphaFold, which relies solely on sequence information, can accurately predict the activated states, from Moldoveanu and colleagues, pages 35 to 47. (portlandpress.com)
  • In doing so, this GEF-facilitated exchange of GDP for GTP is associated with structural changes in the GTP-binding protein which influence the degree to which the GTP-binding protein can interact with other molecules. (justia.com)
  • It is ubiquitously expressed in many tissues and cell types and highly significantly expressed in lung and placenta. (wikipedia.org)
  • RNA in situ hybridization using 33P-labeled riboprobes was used to identify the cell types that expressed Pod-1 in the developing kidney and other tissues. (wikipedia.org)
  • HMGCS2 , the gene that regulates ketone body production, is expressed in liver and several extrahepatic tissues, such as the colon. (aacrjournals.org)
  • Luciferase reporter assays with constructs covering CGI3 sequences in sense and antisense orientation demonstrated that CGI3 harbors a promoter that directs the synthesis of a previously unknown long non-coding RNAs (lncRNAs) in antisense orientation to TCF21. (wikipedia.org)
  • During the transition, C. truncatum probably secretes a range of proteins to establish a compatible interaction with its host, including some that may exclusively be involved in switching the pathogen to the necrotrophic phase. (biomedcentral.com)
  • In an effort to identify novel bHLH factors related to dHAND and eHAND (a novel subclass of cell type-restricted bHLH factors shown to play important roles in cardiac morphogenesis), they screened expressed sequence tag (EST) databases for sequences with homology to the bHLH regions of these factors. (wikipedia.org)
  • This is due to a paucity of functional annotation from sequence homology, which is compounded by low genetic tractability compared with many model organisms. (portlandpress.com)
  • Missense mutations in the splicing factor gene PRPF31 cause a dominant form of retinitis pigmentosa (RP11) with reduced penetrance. (molvis.org)
  • Missense mutations in PRPF31 have previously been shown to cause reduced protein solubility, suggesting insufficiency of functional protein as the disease mechanism. (molvis.org)
  • The RP11 missense mutations exert their pathology mainly via a mechanism based on protein insufficiency due to protein insolubility, but there is also a minor direct negative effect on function. (molvis.org)
  • The authors undertook systematic sequencing of U2AF1 exons in 150 MDS cases, and found that 8.7% had mutations at Ser34. (massgenomics.org)
  • Additionally, we identify sites with shared mutations under directional evolution in the SARS-CoV-2 Spike-encoding protein of VOC and VOI, tracing a heretofore-undescribed correlation with viral spread in South America, India and the USA. (cdc.gov)
  • A new mutation has been reported, making a total of 15 different mutations that can cause premature stop codons in the alpha-L-iduronidase gene ( IDUA ), and the biochemistry of these mutations has been investigated. (medscape.com)
  • CTCF is a highly conserved and essential zinc finger protein expressed in virtually all cell types. (unican.es)
  • When GTP is bound, for instance, Ras proteins can interact with effectors and other molecules to affect cell proliferation, differentiation and apoptosis. (justia.com)
  • Commercially available IGRAs include the ELISA-based Quantiferon TB Gold assays (Cellestis, Victoria, Australia) and the ELISPOT-based T SPOT.TB test (Oxford Immunotec, Abington, UK). (biomedcentral.com)
  • Considering the high protein yield and cost-effectiveness, the S 1-501 -based ELISA could be used as a reliable, sensitive, specific, and economic serological test for PEDV. (biomedcentral.com)
  • Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. (cusabio.com)
  • It was concluded that capsulin heterodimerizes with E12 and binds the specific E-box consensus sequence (CANNTG), though not activating transcription through this sequence on its own. (wikipedia.org)
  • A combination of M.tb specific ESAT-6/CFP-10 fusion protein, Rv2624c and Rv0867c accurately predicted 73% of the TB patients and 80% of the non-TB cases after cross validation. (biomedcentral.com)
  • Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. (caslab.com)
  • A reliable serological assay for evaluating the PEDV-specific humoral and mucosal immune response is important for disease survey, monitoring the efficacy of immunization, and designing strategies for the prevention and control of PED. (biomedcentral.com)
  • Finally, we showed that Bonus SUMOylation is mediated by the SUMO E3-ligase Su(var)2-10, revealing that although SUMOylation of TIF1 proteins is conserved between insects and mammals, both the mechanism and specific site of modification is different in the two taxa. (elifesciences.org)
  • In situ hybridization (ISH) uses complementary DNA or RNA probes to localize a specific DNA or RNA sequence in in tissue sections. (technologypublisher.com)
  • check also tag CHILD or specific HN - 2008 FX - Adolescent Nutrition FX - Infant Nutrition DH - Maternal Nutrition DI - 052505 MN - SP6.021.072 MS - Nutrition of a mother which affects the health of the INFANT as well as herself. (bvsalud.org)
  • Pull-down assays were used to study the interaction between PRPF31 and one of its cognate partners in the spliceosome, PRPF6. (molvis.org)
  • Pull-down assays between PRPF31 and PRPF6 proteins showed a stronger interaction for the mutant than wild type, suggesting a mechanism for the negative effect. (molvis.org)
  • C. truncatum likely secretes an arsenal of proteins that are implicated in maintaining a compatible interaction with its host. (biomedcentral.com)
  • Some of these proteins may have a role in establishing compatible interaction with the host plant. (biomedcentral.com)