• sensitivity
  • The Von Frey assay, introduced by Maximilian von Frey and modified by Weinstein, uses Von Frey hair or fibers, which are small pieces of nylon rod, approximately 50 mm in length, and of varying diameters, to test a rodent's sensitivity to a mechanical stimulus. (wikipedia.org)
  • Two versions of the tail withdrawal assay are commonly employed in pain sensitivity testing. (wikipedia.org)
  • Assay sensitivity is a property of a clinical trial defined as the ability of a trial to distinguish an effective treatment from a less effective or ineffective intervention. (wikipedia.org)
  • Without assay sensitivity, a trial is not internally valid and is not capable of comparing the efficacy of two interventions. (wikipedia.org)
  • Lack of assay sensitivity has different implications for trials intended to show a difference greater than zero between interventions (superiority trials) and trials intended to show non-inferiority. (wikipedia.org)
  • If a trial intended to demonstrate efficacy by showing superiority of a test intervention to control lacks assay sensitivity, it will fail to show that the test intervention is superior and will fail to lead to a conclusion of efficacy. (wikipedia.org)
  • In contrast, if a trial intended to demonstrate efficacy by showing a test intervention is non-inferior to an active control lacks assay sensitivity, the trial may find an ineffective intervention to be non-inferior and could lead to an erroneous conclusion of efficacy. (wikipedia.org)
  • When two interventions within a trial are shown to have different efficacy (i.e., when one intervention is superior), that finding itself directly demonstrates that the trial had assay sensitivity (assuming the finding is not related to random or systematic error). (wikipedia.org)
  • In contrast, a trial that demonstrates non-inferiority between two interventions, or an unsuccessful superiority trial, generally does not contain such direct evidence of assay sensitivity. (wikipedia.org)
  • Assay sensitivity for a non-inferiority trial may depend upon the chosen margin of inferiority ruled out by the trial, and the design of the planned non-inferiority trial. (wikipedia.org)
  • For instance, if there is reliable and reproducible evidence from previous superiority trials of an effect size of 10% for a control intervention compared to placebo, an appropriately designed non-inferiorty trial designed to rule out that the test intervention may be as much as 5% less effective than the control would have assay sensitivity. (wikipedia.org)
  • On the other hand, with this same data, a noninferiority trial designed to rule out that the test intervention may be as much as 15% less effective than the control may not have assay sensitivity, since this trial would not ensure that the test intervention is any more effective than a placebo given that the effect ruled out is larger than the effect of the control compared to placebo. (wikipedia.org)
  • The MiCK assay provides oncologists with a clinically relevant drug-sensitivity profile of tumor cells of an individual cancer patient. (wikipedia.org)
  • formazan
  • Finally, WST assays (1) can be read directly (unlike MTT that needs a solubilization step), (2) give a more effective signal than MTT, and (3) decrease toxicity to cells (unlike cell-permeable MTT, and its insoluble formazan that accumulate inside cells). (wikipedia.org)
  • Examples: Propidium iodide Trypan blue 7-Aminoactinomycin D Mitochondrial activity or caspase assays: Resazurin and Formazan (MTT/XTT) can assay for various stages in the apoptosis process that foreshadow cell death. (wikipedia.org)
  • ATP test Calcein AM Clonogenic assay Ethidium homodimer assay Evans blue Fluorescein diacetate hydrolysis/Propidium iodide staining (FDA/PI staining) Flow cytometry Formazan-based assays (MTT/XTT) Green fluorescent protein Lactate dehydrogenase (LDH) Methyl violet Propidium iodide, DNA stain that can differentiate necrotic, apoptotic and normal cells. (wikipedia.org)
  • Laboratory
  • An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity (the analyte). (wikipedia.org)
  • Since the assay itself (the analytic step) gets much attention, steps that get less attention by the chain of users, i.e. the preanalytic and the post analytic steps, are often less stringently regulated and generally more prone to errors - e.g. preanalytic steps in medical laboratory assays may contribute to 32-75% of all lab errors. (wikipedia.org)
  • In the case of fire assaying of gold and platinum ores, the lengthy time required to carry out an assay generally is offset by carrying out large numbers of assays simultaneously, and a typical laboratory will be equipped with several fusion and cupellation furnaces, each capable of taking multiple samples, so that several hundred analyses per day can be carried out. (wikipedia.org)
  • Enzyme assays are laboratory methods for measuring enzymatic activity. (wikipedia.org)
  • The assay is performed by DiaTech Oncology in its CLAI-certified, CAP-accredited laboratory. (wikipedia.org)
  • enzyme
  • An enzyme-linked immuno-sorbent assay for the detectionand quantitation of anti-tissue Transglutaminase in serum of patients with Celiac Disease and Dermatitis Herpetiformis. (bio-medicine.org)
  • The Emit 2000 Phenytoin Assay is a homogeneous enzyme immunoassay intended for use in the quantitative analysis of phenytoin in human serum or plasma. (bio-medicine.org)
  • The assay usually aims to measure an intensive property of the analyte and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of some effect in comparison to a standard, etc. (wikipedia.org)
  • Cytolysis or membrane leakage assays: This category includes the lactate dehydrogenase assay, a stable enzyme common in all cells which can be readily detected when cell membranes are no longer intact. (wikipedia.org)
  • All enzyme assays measure either the consumption of substrate or production of product over time. (wikipedia.org)
  • proliferation
  • Hi, I wonder if somebody has already noted different results between the Promega MTS proliferation assay and the classical counting technique. (bio.net)
  • Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cytostatic activity (shift from proliferation to quiescence) of potential medicinal agents and toxic materials. (wikipedia.org)
  • dilution
  • The HI assay is generally performed by creating a dilution series of antiserum across the rows of a 96-well microtiter plate. (wikipedia.org)
  • The term dilution assay is generally used to designate a special type of bioassay in which one or more preparations (e.g. a drug) are administered to experimental units at different dose levels inducing a measurable biological response. (wikipedia.org)
  • Dilution assays can be direct or indirect. (wikipedia.org)
  • In a direct dilution assay the amount of dose needed to produce a specific (fixed) response is measured, so that the dose is a stochastic variable defining the tolerance distribution. (wikipedia.org)
  • Conversely, in an indirect dilution assay the dose levels are administered at fixed dose levels, so that the response is a stochastic variable. (wikipedia.org)
  • For a mathematical definition of a dilution assay an observation space U {\displaystyle U} is defined and a function f : U → R {\displaystyle f:U\rightarrow \mathbb {R} } so that the responses u ∈ U {\displaystyle u\in U} are mapped to the set of real numbers. (wikipedia.org)
  • Of notable importance in this respect is the theory of Generalized Linear Models with which a wide range of dilution assays can be modelled. (wikipedia.org)
  • reagents
  • The assay kits are all-inclusive systems, including optimized media for use during cell culture and in preparing test compound treatments, a LRH-1 reference agonist, luciferase detection reagents, and an assay plate. (prweb.com)
  • If the assay involves addition of exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity (and quality) of the target is the only limiting factor for the reaction/assay process, and the difference in the assay outcome is used to deduce the unknown quality or quantity of the target in question. (wikipedia.org)
  • bullion
  • They are not involved in hallmarking, as there has never been a hallmarking scheme in the U.S. In the 1800s, the functions of assay offices in the U.S. included receiving bullion deposits from the public and from mining prospectors in the various American territories. (wikipedia.org)
  • The assay offices that still operate today function solely within national coining system (including bullion coinage for sales to investors). (wikipedia.org)
  • This method is better suited for the assay of bullion and gold stocks rather than works of art or jewelry because it is a completely destructive method. (wikipedia.org)
  • Methods
  • A large number of different methods of measuring the concentrations of substrates and products exist and many enzymes can be assayed in several different ways. (wikipedia.org)
  • analyze
  • They were precisely quantified using highly sensitive digital PCR assays to analyze amplification of EGFR, MET, FGFR3, MYC, ERBB2, and MYCN genes at +3, +6, and +12 copies against a single well-characterized genomic background, the company said. (genomeweb.com)
  • Although this technique can provide accurate results, the assay is time-consuming to set up and analyze and can only provide data on tumor cells that can grow in culture. (wikipedia.org)
  • detection
  • Tissue microarray for analyzing multiple tissue samples Cellular microarray for observing cellular responses against a panel of materials Chemical compound microarray to assay multiple chemical compounds for specific activities Multiplex detection enables the simultaneous detection of two or more targets on a western blot. (wikipedia.org)
  • For detection of cells general staining techniques (e.g. trypan blue) or special probes (e.g. mt-dehydrogenase detection with MTT assay) are used. (wikipedia.org)
  • purity
  • For assay of currency coins, this literally meant analysis of the purity of the gold or silver or whatever precious component was used to represent the true value of the coin. (wikipedia.org)
  • Functional
  • Functional assays: Assays of cell function will be highly specific to the types of cells being assayed. (wikipedia.org)
  • For transplantable whole organs the ultimate assay is the ability to sustain life after transplantation, an assay which is not helpful in preventing transplantation of non-functional organs. (wikipedia.org)
  • Multiplex assays are widely used in functional genomics experiments that endeavor to detect or to assay the state of all biomolecules of a given class (e.g., mRNAs, proteins) within a biological sample, to determine the effect of an experimental treatment or the effect of a DNA mutation over all of the biomolecules or pathways in the sample. (wikipedia.org)
  • Test
  • test the quality of", from Anglo-French assaier, from assai (n.), from Old French essai "trial", and the noun assay thus means "trial, test of quality, test of character", mid-14th century, from Anglo-French assai and the meaning "analysis" is from the late 14th century. (wikipedia.org)
  • It is unclear whether the process is really considered noxious versus simply annoying, so this assay is a test of mechanical nociception or simply mechanical sensibility. (wikipedia.org)
  • The primary advantage of tail withdrawal assays to other forms of thermal nociception testing, such as the hot-plate test or Hargreaves test, is the relative stability of results with repeated observations. (wikipedia.org)
  • 1955
  • Coinage operations were suspended in March 1955, but the plant continued to operate as an Assay Office. (wikipedia.org)
  • Fragment
  • The HpaII tiny fragment Enrichment by Ligation-mediated PCR Assay (HELP Assay) is one of several techniques used for determining whether DNA has been methylated. (wikipedia.org)
  • compounds
  • ASSAY and Drug Development Technologies delivers access to the techniques and tools that enable new advances in early-stage screening, and lead to therapeutic compounds for drug discovery and development. (liebertpub.com)
  • However this convenience makes the MTS assay susceptible to colormetric interference as the intermittent steps in the MTT assay remove traces of coloured compounds, whilst these remain in the microtitre plate in the one-step MTS assay. (wikipedia.org)
  • clinical
  • Both have been built with biosynthetic technology that can be easily scalable to incorporate new CNVs over time, and were developed so that clinical labs conducting NGS-based tumor profiling for cancer can better understand and characterize their assays, SeraCare said. (genomeweb.com)
  • results
  • Full-Service - Assay Services staff performs all determinations and results and validations are sent to the investigators electronically. (wisc.edu)
  • Precautions are needed to ensure accuracy when using this assay and there are strong arguments for confirming MTS results using qualitative observations under a microscope. (wikipedia.org)
  • X-ray Fluorescence assay is not as accurate as fire-assay but dependent on the spectrometer used, can achieve results of within 1 percent. (wikipedia.org)
  • One significant limitation of thermal assays lies in the specificity and validity of results in animals as models of human pain. (wikipedia.org)
  • gene
  • The assay has been designed for retrospective gene expression profiling to complement traditional immunohistochemistry (IHC) testing. (genomeweb.com)
  • molecular
  • HTG Molecular Diagnostics plans to launch the HTG EdgeSeq PATH Assay for research use only by the end of the second quarter. (genomeweb.com)
  • A molecular assay, Trofile was developed by Monogram Biosciences for use in HIV treatment. (wikipedia.org)
  • generally
  • But generally, assays involve biological material or phenomena which tend to be intrinsically more complex either in composition or in behavior or both. (wikipedia.org)
  • stages
  • Additionally, as described before, this assay produces a response in two discrete stages, allowing researchers to model both acute and tonic pain using a single noxious chemical. (wikipedia.org)
  • measure
  • Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. (cancer.gov)
  • These assays measure the existence of pain through behaviors such as withdrawal, licking, immobility, and vocalization. (wikipedia.org)
  • different
  • WSTs (water-soluble tetrazolium salts) are a series of other water-soluble dyes for MTT assays, developed to give different absorption spectra of the formed formazans. (wikipedia.org)
  • assess
  • Viability assays are used to assess the success of cryopreservation techniques, the toxicity of substances, or the effectiveness of substances in mitigating effects of toxic substances. (wikipedia.org)
  • Type
  • To perform the MiCK assay, the oncologist will send a sample of the specimen (the type of the specimen depends on the type of the cancer) to DiaTech Oncology which will isolate and purify the tumor cells. (wikipedia.org)
  • Development and characterization of a novel single-cycle recombinant virus assay to determine human immunodeficiency virus type 1 coreceptor tropism. (wikipedia.org)
  • analysis
  • An assay (analysis) is never an isolated process and must be combined with pre- and post-analytic procedures. (wikipedia.org)
  • Colorimetry Water chemistry analysis Water testing anionic surfactants Alison L. George, Graham F. White "Optimization of the methylene blue assay for anionic surfactants added to estuarine and marine water" Environmental Toxicology and Chemistry 1999, Volume 18, pages 2232-2236. (wikipedia.org)
  • https
  • A schematic of a toeprinting assay can be found here: https://www.researchgate.net/figure/41124064_fig2_A-Schematic-of-the-primer-extension-inhibition-toeprint-assay-illustrating-how. (wikipedia.org)
  • cells
  • On the other hand, older generation qualitative assays, especially bioassays, may be much more gross and less quantitative (e.g., counting death or dysfunction of an organism or cells in a population, or some descriptive change in some body part of a group of animals). (wikipedia.org)
  • For Cell-killing Particle assays, the surviving fraction of cells is used to approximate the Poisson Distribution of virus particles amongst cells and therefore determine the number of CKPs encountered by each cell. (wikipedia.org)
  • A viability assay is an assay to determine the ability of organs, cells or tissues to maintain or recover viability. (wikipedia.org)
  • If the cells do not have high intracellular potassium and low intracellular sodium, then (1) the cell membrane may not be intact, and/or (2) the sodium-potassium pump may not be operating well As with many kinds of viability assays, quantitative measures of physiological function do not indicate whether damage repair and recovery is possible. (wikipedia.org)
  • Red blood cells have been assayed in terms of deformability, osmotic fragility, hemolysis, ATP level, and hemoglobin content. (wikipedia.org)
  • Resazurin Trypan Blue, a living-cell exclusion dye (dye only crosses cell membranes of dead cells) TUNEL assay Cytotoxicity Vital stain Pegg DE (1989). (wikipedia.org)
  • Chemotaxis assays are experimental tools for evaluation of chemotactic ability of prokaryotic or eukaryotic cells. (wikipedia.org)
  • Labelled (e.g. fluorochromes) cells are also used, in some assays cells get labelled during transmigration the filter. (wikipedia.org)
  • content
  • Where required to be hallmarked, semi-finished precious metal items of art or jewelry pass through the official testing channels where they are analyzed or assayed for precious metal content. (wikipedia.org)
  • Office
  • It served as an Assay Office until 1906, when coinage operations began. (wikipedia.org)
  • In 1962, its official designation was changed from Mint to Assay Office. (wikipedia.org)
  • It is operated as an adjunct of the New York Assay Office. (wikipedia.org)
  • After the Civil War, the plant was reopened in 1868 as an Assay Office until 1913, when the plant was closed. (wikipedia.org)
  • The facility operated as an Assay Office from 1909 until 1942, when it was closed. (wikipedia.org)
  • There are four remaining Assay Offices in the UK: London Assay Office Sheffield Assay Office Birmingham Assay Office Edinburgh Assay Office Dublin (now in the Republic of Ireland) Exeter (closed 1883) Chester (closed 24 August 1962) Glasgow (closed 31 March 1964) Newcastle (closed 1884) Norwich (closed 1702) York (closed 1857) The Irish assay system is based on that of the UK. (wikipedia.org)
  • There is one assay office, the Dublin Assay Office in Dublin. (wikipedia.org)
  • usually
  • MTT assays are usually done in the dark since the MTT reagent is sensitive to light. (wikipedia.org)
  • A nociception assay (nocioception or nocioperception assay) evaluates the ability of an animal, usually a rodent, to detect a noxious stimulus such as the feeling of pain, caused by stimulation of nociceptors. (wikipedia.org)
  • Pain-reflex latency observations from other tests are usually much more variable both across and within subjects than those obtained from the tail withdrawal assay. (wikipedia.org)
  • cell
  • INDIGO's LRH-1 assay kits are cell-based genetic reporter assay systems. (prweb.com)
  • The MTS assay is often described as a 'one-step' MTT assay, which offers the convenience of adding the reagent straight to the cell culture without the intermittent steps required in the MTT assay. (wikipedia.org)
  • It is important to keep in mind that assay conditions can alter metabolic activity and thus tetrazolium dye reduction without affecting cell viability. (wikipedia.org)
  • An assay of the ability of a cell line to adhere and divide may be more indicative of incipient damage than membrane integrity. (wikipedia.org)
  • One Cell Systems - Gel Microdrop (GMD) Secretion Assay technology. (wikipedia.org)
  • ability
  • The ability to perform such multiplex assay experiments measuring large numbers of biomolecular analytes has been facilitated by the completion of the human genome sequence and that of many other model organisms. (wikipedia.org)
  • Research
  • INDIGO offers both screening services and assay kits for the pharmaceutical, biotechnology, agri-business, nutriceutical industries, as well as government research agencies and academic researchers. (prweb.com)
  • Self-Service - the investigator or research personnel work with Assay Services staff to perform determinations. (wisc.edu)
  • Those assays that are very highly commercially demanded have been well investigated in research and development sectors of professional industries, undergone generations of development and sophistication, and become copyrighted intellectual properties via highly competitive process patenting. (wikipedia.org)
  • Item
  • Title 15, Chapter 8, Section 291 of the United States Code makes it unlawful to stamp goods in the United States with "United States assay" or any similar stamp which gives the impression that the item has been officially assayed by the United States government. (wikipedia.org)
  • often
  • Such industrial scale assays as these are often done in well equipped laboratories and with automated organization of the procedure-from ordering an assay to pre-analytic sample processing (sample collection, necessary manipulations e.g. spinning for separation or other processes, aliquoting if necessary, storage, retrieval, pipetting/aspiration etc. (wikipedia.org)
  • In the past the assay was conducted by using the touchstone method but currently (most often) it is done using X-ray fluorescence (XRF). (wikipedia.org)
  • Time
  • The (HTAB, O-Dianisidine HCl) assay is working very well but I'm having some trouble in converting the slope, change in OD/time, into MPO units. (bio.net)
  • perform
  • The information communication (e.g. request to perform an assay and further information processing) or specimen handling (e.g. collection, transport and processing) that are done until the beginning of an assay are the preanalytic steps. (wikipedia.org)