• Similarity searches of nucleotide and protein sequence databases revealed some evidence of homology with a number of expressed sequence tags (ESTs) and a Caenorhabditis elegans protein, which shows a slight similarity with Arabidopsis thaliana ubiquitin-like protein 8 [ 8 ] . (medscape.com)
  • To understand the regulatory mechanisms underlying the biogenesis of photosystem II (PSII) we have characterized the nuclear mutant hcf136 of Arabidopsis thaliana and isolated the affected gene. (nih.gov)
  • This fungal species is known to have narrower host range compared to V. dahliae with preference for host species within the family Brassicaceae , including Brassica species and Arabidopsis thaliana [ 7 , 8 ]. (biomedcentral.com)
  • Analysis of pathogen-induced glutathione S-transferases in Arabidopsis thaliana and a gene related to systemic acquired resistance in cucumber (Cucumis sativus L. (unifr.ch)
  • We have first analyzed the expression of two members of the glutathione S-transferase (GST) multigene family, AtGSTF2 and AtGSTF6 in Arabidopsis thaliana inoculated with an avirulent strain of Pseudomonas syringae. (unifr.ch)
  • Other approaches such as the analysis of transgenic Arabidopsis thaliana expressing CUPI1 might be promising and could provide an answer to the function of the protein in vivo. (unifr.ch)
  • A comparative molecular analysis of nucleotide sequence in NCBI databases showed that it shared 95% homology with the gene of Arabidopsis thaliana (MIP-C), with a theoretical isoelectric point of 8.84. (nefu.edu.cn)
  • Nonexpressor of Pathogenesis Related 1 (NPR1) is an important regulatory component of systemic acquired resistance (SAR) in Arabidopsis thaliana . (beds.ac.uk)
  • 100 times smaller genome of Arabidopsis thaliana , and there is no evidence of a recent whole-genome duplication in the gymnosperm lineage. (nature.com)
  • Antibodies raised to LTPs protein from Daucus carota and Arabidopsis thaliana recognized a protein of 10 kDa that was expressed in both the rachis and abscission zone tissues of S. nigra before and after ethylene treatment. (massey.ac.nz)
  • Here, for example, on the same idea of using DNA differences, is a map based on this restriction fragment like the polymorphisms of Arabidopsis thaliana, a small plant that's becoming increasingly important as a genetical organism. (nih.gov)
  • This fungal species is thought to be of a hybrid origin, having a genome composed of combinations of lineages denominated A and D. In this study we report the draft genomes of 2 V. longisporum field isolates sequenced using the Illumina technology. (biomedcentral.com)
  • Phylogenetic methods which do not rely on multiple sequence alignments are important tools in inferring trees directly from completely sequenced genomes. (biomedcentral.com)
  • Here, we extend the recently described Genome BLAST Distance Phylogeny (GBDP) strategy to compute phylogenetic trees from all completely sequenced plastid genomes currently available and from a selection of mitochondrial genomes representing the major eukaryotic lineages. (biomedcentral.com)
  • Nowadays, an increasing number of completely sequenced genomes are available and a growing field of phylogenetic research deals with the question of how to infer reliable phylogenies from this large amount of data to overcome the limitations of single-gene phylogenies. (biomedcentral.com)
  • A relatively obvious approach to phylogenetic analysis of whole genomes is to extract as many genes as possible from the genome sequences, create a multiple sequence alignment from each of the genes and to concatenate all alignments. (biomedcentral.com)
  • Recent advances in the cost and utility of second generation, high-throughput sequencing technologies have made it possible for ten conifer reference genomes to be assembled ( http://www.pinegenome.org/pinerefseq ). (plos.org)
  • Two putative homologs of NPR1 gene were found in the two sequenced grapevine genomes available in the Genoscope database for line 40024 and in the IASMA database for Pinot noir ENTAV 115. (beds.ac.uk)
  • Thus, no full genome sequence of a gymnosperm species is available at present, whereas 30 angiosperm and more basal plant genomes have been sequenced. (nature.com)
  • However, size is not the only challenge to sequencing presented by conifer genomes. (nature.com)
  • The availability of conifer genome sequences would enable comparative analyses of genome architecture and the evolution of key traits for seed plants, including flower or fruit development and life history (perennial versus annual), and help to determine how and why conifer genomes became so large. (nature.com)
  • We applied a homology-based approach to sequenced cereal genomes, in order to finally trace the mutual evolution of the structure of the CENH3 genes and the nearby regions in various tribes. (biomedcentral.com)
  • DNA regions with homology to histone H3 genes have been found in the genomes of primitive organisms, such as unicellular algae, and H3 gene families have expanded and diversified across plant evolution to reach 14-15 genes in land plants, such as maize and rice [ 10 ]. (biomedcentral.com)
  • Cause of course compared to small genomes like a bacteriophage or E. coli, the human genome is a huge genome and has three billion base pairs of DNA, and it's considerably less convenient to work with than prokaryotic genomes because of the amount of repetitive sequence and other nasty things about it. (nih.gov)
  • Branched-chain amino acids (BCAA) are ubiquitous in many organisms, comprising 35% of all proteins and 40% of the amino acids required in all mammals. (wikipedia.org)
  • In the past decade the first Arabidopsis genes encoding cytoskeletal proteins were identified. (bioone.org)
  • Most of these sequences are uncharacterized and were not identified as related to cytoskeletal proteins. (bioone.org)
  • Several Arabidopsis ABPs, MAPs, and IF proteins are represented by individual genes and most were represented as as small gene families. (bioone.org)
  • Electrophoretic mobility shift assay established the binding of these homeobox proteins with specific DNA sequences, AH1 (CAAT(A/T)ATTG) and AH2 (CAAT(C/G)ATTG). (frontiersin.org)
  • Meanwhile, the C-terminal sequence of GRAS transcription factors is relatively conserved, has specific transcription regulatory domains that bind to DNA or other proteins, and can recognize a variety of different target receptors. (biomedcentral.com)
  • The posterior segment of the LHR II domain contains Leucine-X-X-Leucine-Leucine (LXXLL, where X stands for any amino acid) structure and is conserved in most GRAS proteins [ 11 ]. (biomedcentral.com)
  • Comparison of the amino acid sequences of the proteins of the listed classes demonstrated no significant structural homology among them. (atoom.ru)
  • The deduced amino acid sequences share structural features with known NPR1-like proteins: ankyrin repeats, BTB/POZ domains, nuclear localization signature and cysteines. (beds.ac.uk)
  • Phylogenetic analyses of deduced amino acid sequences show that VvNPR1.1 belongs to a first group of NPR1 proteins known as positive regulators of SAR and VvNPR1.2 belongs to a second group of NPR1 proteins whose principal members are AtNPR3 and AtNPR4 defined as negative regulators of SAR. (beds.ac.uk)
  • The specificity of the proteins is determined by the sequences outside the repeats themselves. (embl.de)
  • We chose proteins that include amino and carboxyl extensions as well as proteins that are made up entirely of WD-repeats. (embl.de)
  • The purpose of the present study was to clarify the effect of different periods of antibiotic administration on the hepatic bile acid profile and expression of pharmacokinetic-related proteins in mouse liver, kidney, and brain capillaries. (inrae.fr)
  • The nitrilase from Aspergillus niger K10 is highly homologous (≥86%) with proteins deduced from gene sequencing in Aspergillus and Penicillium genera. (biomedcentral.com)
  • Arabidopsis proteins. (lookformedical.com)
  • Three of the putative OZ-specific proteins (designated OZ10, OZ28 and OZ43) were trypsin-digested and some initial amino acid sequence data obtained. (massey.ac.nz)
  • Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments. (lookformedical.com)
  • Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. (lookformedical.com)
  • Andrej Sali presented the MODBASE of comparative structure models (which provides fold assignments for about 56% of all proteins), described the strategy to exploit structurally unknown (un-modeled) families for target selection, and estimated that the number of new structures needed to cover all 6190 PfamA families ranges from 4000 (allowing for any level of similarity) to 25,000 (with a threshold of at least 80aa long at 30% sequence identity). (nih.gov)
  • Converged from the different estimations based on the current fold space of non-randomly sampled protein domains, a rough approximation of between 10,000 to 20,000 structures seem to be required for 50% of amino acid residue coverage of the entire universe of proteins. (nih.gov)
  • Title: General Amino Acid Control and 14-3-3 Proteins Bmh1/2 Are Required for Nitrogen Catabolite Repression-Sensitive Regulation of Gln3 and Gat1 Localization. (genscript.com)
  • Despite their prevalence and importance, the genome sequences of loblolly pine, Norway spruce, and white spruce, three ecologically and economically important conifer species, are just becoming available to the research community. (plos.org)
  • All previously reported eukaryotic nuclear genome sequences have been incomplete, especially in highly repeated units and chromosomal ends. (biomedcentral.com)
  • Bombyx mori, the domesticated silkworm, is a major insect model for research, and the first lepidopteran for which draft genome sequences became available in 2004. (inrae.fr)
  • The transcript levels of these genes were enhanced significantly in the presence of plant hormones, including abscisic acid (ABA), auxin, salicylic acid, and gibberellic acid. (frontiersin.org)
  • Difficulties with this approach may arise if orthologous genes cannot be identified with certainty or if the combined sequence length is still too small to give well-resolved trees. (biomedcentral.com)
  • This form of resistance, called systemic acquired resistance (SAR), is dependent of the local and systemic accumulation of salicylic acid (SA), which is involved in the concomitant activation of genes associated with defense against certain types of microorganisms. (unifr.ch)
  • Besides SA, the plant hormones jasmonic acid (JA) and ethylene have been shown to be involved in the activation of separate sets of genes, the products of which mediate resistance against other types of pathogens. (unifr.ch)
  • Sequence and Expression Analysis of MYB Family Genes in Betula platyphylla Suk. (nefu.edu.cn)
  • Together, this sequence represents 280 Mbp (roughly 1% of the loblolly pine genome) and one of the most comprehensive studies of repetitive elements and genes in a gymnosperm species. (plos.org)
  • We evaluated PC genes and protein sequences from 6 plants namely, Brassica sp. (bbrjournal.com)
  • 1999) Phytochelatin Synthase Genes From Arabidopsis And The Yeast Schiz-osaccharomyces pombe. (bbrjournal.com)
  • Our earlier, nearly complete genome sequence of the hot-spring red alga Cyanidioschyzon merolae revealed several unique features, including just three ribosomal DNA copies, very few introns, and a small total number of genes. (biomedcentral.com)
  • Analysing the flanking sequences surrounding genes of interest is often highly relevant to understanding the role of mobile genetic elements (MGEs) in horizontal gene transfer, particular for antimicrobial resistance genes. (sachecucine.it)
  • Analysing the flanking sequences surrounding genes of interest is often highly relevant to understanding the role of mobile genetic elements (MGEs) in horizontal …Flanking sequences from approximately half of these insertions correspond to the IAAH gene. (sachecucine.it)
  • RNAP will preferentially release its RNA transcript at specific DNA sequences encoded at the end of genes known as terminators . (wikidoc.org)
  • The goals of this study were to identify expressed sequence tags (EST) in Citrus sinensis (L.) Osbeck corresponding to genes involved in general phenylpropanoid biosynthesis and the key genes involved in the main flavonoids pathways (flavanones, flavones, flavonols, leucoanthocyanidins, anthocyanins and isoflavonoids). (scielo.br)
  • Structural studies of human branched-chain amino acid aminotransferases (hBCAT) revealed that the peptide bonds in both isoforms are all trans except for the bond between residues Gly338-Pro339. (wikipedia.org)
  • Although the CXXC motif is present only in mammalian BCATs, the surrounding amino acid residues were found to be highly conserved in both prokaryotic and eukaryotic cells. (wikipedia.org)
  • Small sequence motifs and many of the cysteine residues were conserved between the two sequences. (unifr.ch)
  • Furthermore, we identify key residues responsible for both the evasion of perception by flg22 Rso in Arabidopsis and the gain of perception by the soybean receptors. (nature.com)
  • The analysis of the crystal structure of Arabidopsis FLS2 and BAK1 ectodomains in complex with flg22 revealed that FLS2 LRRs bind specific residues of flg22, while BAK1 recognizes the C-terminus of FLS2-bound flg22 5 . (nature.com)
  • In particular, there is a higher degree of homology of your amino acid residues in the active web page. (bet-bromodomain.com)
  • Amongst them, 5 amino acid residues, Phe409 and Phe452, which type a stacking interaction with HPPD inhibitors, and His254, His336, and Glu422, that are vital for enzyme activity because they kind a bidentate interaction with Fe(II), had been fully conserved inside the plants. (bet-bromodomain.com)
  • Additionally, two amino acid residues involved in interactions exceptional to fenquinotrione, Phe420 and Gln335, were also conserved (Supplemental Fig. S1). (bet-bromodomain.com)
  • Based on the SCOP structural domains and superfamily classification, Cyrus Chothia showed that (i) structural assignment is possible for about 60% of sequences (or 50% of residues), (ii) distribution of family sizes obeys the power law in which a small number of superfamilies cover large protein space, and (iii) a large fraction (about 75%) of superfamilies have broad evolutionary distribution, containing both prokaryotes and eukaryotes. (nih.gov)
  • To support a functional annotation of lab data, such as protein sequences, metabolites or DNA sequences as well as a semi-automated data exploration in information retrieval environments, an integrated view to databases is essential. (degruyter.com)
  • All sequences were aligned based on CLUSTALW matrix for DN sequences and MUSCLE algorithm for protein sequences. (bbrjournal.com)
  • In addition, protein sequences also showed the high diversity among species and it is still clustered based on their genus. (bbrjournal.com)
  • The latter possibility is more likely, considering a relatively lower homology of LRR and 70-amino-acid-island sequences between rice OsBRI1 and Arabidopsis BRI1 kinases. (sideload.com)
  • 1. Inhibitory effects of fenquinotrione around the HPPD activity of recombinant Arabidopsis and rice HPPD. (bet-bromodomain.com)
  • A lot more than 80 identity with rice HPPD amongst monocots and more than 70 identity with Arabidopsis HPPD amongst dicots was observed. (bet-bromodomain.com)
  • The experimental results demonstrated that CDL-PCR is an efficient and flexible method for identifying the flanking sequences in transgenic rice and Arabidopsis …In this protein family, a conserved core domain, the so-called α-crystallin or Hsp20 domain, is flanked by highly variable, nonconserved sequences that are essential for chaperone function. (sachecucine.it)
  • Both sequences share similarity to FAD2 desaturases and FAD2-related enzymes. (researchgate.net)
  • Nucleotide sequence of the P. obesus beacon cDNA. (medscape.com)
  • Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1. (ncsu.edu)
  • It is also called nucleotide sequence. (lookformedical.com)
  • The nucleotide sequence of Saccharomyces cerevisiae chromosome IV. (genscript.com)
  • databases agreed to adopt the NCBI taxonomy as the standard classification for the nucleotide sequence databases. (nih.gov)
  • However, flagellin from Ralstonia solanacearum , the causal agent of the bacterial wilt disease, presents a polymorphic flg22 sequence (flg22 Rso ) that avoids perception by all plants studied to date. (nature.com)
  • Given the importance of the flagellum for bacterial lifestyle, the abundance of flagellin (the major protein that builds the flagellum), and the conservation of certain peptides in its sequence, flagellin constitutes an excellent tell-tale molecule for animals and plants to detect the presence of a potential bacterial pathogen. (nature.com)
  • Using PCR with sequence-specific primers to amplify portions of the C. merolae bacterial artificial chromosomes (BACs) that contained gap regions of interest, and then sequencing the resulting subclones, we have reduced the number of gaps between contigs from 46 to zero (Figure 1 ). (biomedcentral.com)
  • Apart from the teleomorph/anamorph pair Gibberella/Fusarium , the Aspergillus genus is a rich source of these enzymes, which exhibit low homology to bacterial nitrilases and thus may differ in their catalytic properties. (biomedcentral.com)
  • This protein appears to be an aldose-1-epimerase-like enzyme (otherwise known as mutarotase) from its homology to bacterial forms of mutarotase. (massey.ac.nz)
  • This model describes bacterial and chloroplast forms of the 50S ribosomal protein L28, a polypeptide about 60 amino acids in length. (unl.edu)
  • Combination of large scale genomic sequencing, cDNA selection and computer-aided analysis led to the characterization of two transcriptional units. (nih.gov)
  • In this paper the gene encoding the Tamarix albiflonum Aquaporin (AQP) was amplified by 5′rapid amplification of cDNA end (RACE) based on the sequence information obtained from the expressed sequence tag of subtractive hybridization library constructed under PEG6000 stress. (nefu.edu.cn)
  • The cDNA of the Tamarix albiflonum AQP gene is 1043 bp long, encoding a protein of 287 amino acids with a predicted molecular mass of 30.9KDa,has 6 transmembrane regions, and possessing the MIP family signal consensus sequence SGXHXNPAVT and the higher plant PIP highly conservative sequence GGGANXXXXGY and TGI/TNPARSL/ FGAAI/VI/VF/YN. (nefu.edu.cn)
  • The following BMH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • These sequences represent the protein coding region of the BMH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • Over-expression of OsHOX24 imparted higher sensitivity to stress hormone, ABA, and abiotic stresses in the transgenic Arabidopsis plants as revealed by various physiological and phenotypic assays. (frontiersin.org)
  • In this study, the T-DNA insertion sites and flanking sequences were identified in two newly developed transgenic glyphosate-tolerant soybeans GE-J16 and ZH10-6 based on whole genome sequencing (WGS) method. (sachecucine.it)
  • Amino acid sequences deduced from human and mouse ESTs showed 100% homology with the P . obesus beacon, indicating that this protein is highly conserved between species. (medscape.com)
  • The development of whole-genome sequencing has allowed the GRAS gene family to be identified and characterized in many species. (biomedcentral.com)
  • But it is clustered for the same species has similar sequence. (bbrjournal.com)
  • Sequence analyses show that NPR1.1 and NPR1.2 are highly conserved among the different accessions not only V. vinifera cultivars but also other species. (beds.ac.uk)
  • Selected data were further modeled using SWISSMODEL to evaluate the 3D-structural analysis based on homology modeling. (bbrjournal.com)
  • We concluded that structural of PC gene is more conserved than its sequences. (bbrjournal.com)
  • The two domains have high structural homology despite minimal sequence homology. (unl.edu)
  • Burkhard Rost presented an integrated target selection strategy that involves automatic identification of domain regions with no homology models, manual refinements to select targets with poor models or predicted novel function, followed by functional annotation of new structures base on structural features. (nih.gov)
  • Opening speaker Wayne Hendrickson offered a definition of structural genomics as 'structure determination on a pan-genome scale aiming to provide representative structures for all sequence families in all living organisms. (nih.gov)
  • Analysis of 8714 sHsps revealed a broad variation of primary sequences within the superfamily as well as phyla-dependent differences.Molecular characterization of sequence flanking exogenous fragment insertion is essential for safety assessment and labeling of genetically modified organism (GMO). (sachecucine.it)
  • When new sequences are submitted to GenBank, the submission is checked for new organism names, which are then classified and added to the Taxonomy database. (nih.gov)
  • Furthermore, the use of concatenated multiple sequence alignments discards information that can be utilised by other methods of phylogenetic inference. (biomedcentral.com)
  • Comparison of amino acid sequences of HPPDs Phylogenetic analysis of the amino acid sequence of plant HPPDs showed that monocotyledonous and dicotyledonous plants were divided into two clades (Fig. three). (bet-bromodomain.com)
  • Two independent data sets from whole-genome shotgun sequencing were merged and assembled together with newly obtained fosmid- and BAC-end sequences. (inrae.fr)
  • These results suggest that bile acids and metabolizing enzymes in the liver are affected in a period-dependent manner by antibiotic treatment, while the blood-brain barrier and kidneys are less affected. (inrae.fr)
  • Enzymes Nit-ANigRec and Nit-ANigWT differed in substrate specificity, acid/amide ratio, reaction optima and stability. (biomedcentral.com)
  • The amino acid sequence shows strong homology with enzymes of the cNOR class. (unl.pt)
  • One encodes the already known BENE protein, and the other encodes a novel protein of at least 732 amino acids containing a putative src homology 3 domain. (nih.gov)
  • In this study, the gene encoding this enzyme was amplified, cloned and sequenced and the protein deduced from gene sequencing was found to be highly homologous with a number of putative nitrilases in Aspergillus and Penicillium . (biomedcentral.com)
  • We used chimeric single-guide RNA (cgRNA) molecules carrying both sequences for target site specificity (to generate the double-strand breaks) and repair template sequences (to direct HDR), flanked by regions of homology to the target. (frontiersin.org)
  • This is also illustrated by previous transcriptomic analysis showing that AtAHL13 is a key factor regulating jasmonic acid biosynthesis signal transduction and pathogen immunity [ 16 ]. (biomedcentral.com)
  • Besides SA-mediated SAR, other pathways are involved in plant defence systems, namely ISR (induced systemic resistance) and jasmonic acid (JA)-mediated pathways. (beds.ac.uk)
  • WD-40 repeats (also known as WD or beta-transducin repeats) are short ~40 amino acid motifs, often terminating in a Trp-Asp (W-D) dipeptide. (embl.de)
  • Purifying selection acts mostly on αCENH3s, while βCENH3s form more heterogeneous structures, in which clade-specific amino acid motifs are present. (biomedcentral.com)
  • For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. (lbl.gov)
  • Expression of CoFac2 in yeast revealed that the encoded enzyme acts as a fatty acid conjugase converting 18:2(9Z, 12Z) to calendic acid 18:3(8E, 10E, 12Z). (researchgate.net)
  • Furthermore, AtGSTF2 expressed in E.coli led to active glutathione peroxidase activity, capable to detoxify fatty acid hydroperoxides in vitro. (unifr.ch)
  • A histone methylation-dependent DNA methylation pathway is uniquely impaired by deficiency in Arabidopsis S-adenosylhomocysteine hydrolase. (nih.gov)
  • it is 69% identical to the hydrolase from Arabidopsis ( Q9LKJ1 ). (lbl.gov)
  • The biological function of branched-chain amino acid aminotransferases is to catalyse the synthesis or degradation of the branched chain amino acids leucine, isoleucine, and valine. (wikipedia.org)
  • RNAP can initiate transcription at specific DNA sequences known as promoters . (wikidoc.org)
  • In its current form, IAP supports the investigation of Maize, Barley and Arabidopsis plants based on images obtained in different spectra. (degruyter.com)
  • Branched-chain amino acid aminotransferase (BCAT), also known as branched-chain amino acid transaminase, is an aminotransferase enzyme (EC 2.6.1.42) which acts upon branched-chain amino acids (BCAAs). (wikipedia.org)
  • The BCAT enzyme catalyzes the conversion of BCAAs and α-ketoglutarate into branched chain α-keto acids and glutamate. (wikipedia.org)
  • Moreover, an in vitro enzyme activity experiment revealed that the 241st amino acid residue was the key residue. (bvsalud.org)
  • The latter enzyme was thus truncated by 46 amino acids. (biomedcentral.com)
  • CuPi1 encodes a small protein of 87 amino acids with homology to ω-conotoxin of a sea snail (Conus geographus) venom toxin. (unifr.ch)
  • To achieve these goals will require structure determination at different levels of granularity (i.e., more structures for large, diverse, and functionally important protein families such as protein kinases) and improvement in the quality of homology modeling to cover broader sequence space. (nih.gov)
  • Following the completion of these large assemblies, annotation efforts will be undertaken to characterize the reference sequences. (plos.org)
  • Annotation: the most common InterPro annotation(s) of the Arabidopsis members in the family. (cornell.edu)
  • Comparative sequencing of Pinus sylvestris , Abies sibirica , Juniperus communis , Taxus baccata and Gnetum gnemon reveals that the transposable element diversity is shared among extant conifers. (nature.com)
  • Inheritance, homology by common descent, and the conservation of sequence and structure in the determination of function are all central ideas in biology that are directly related to the evolutionary history of any group of organisms. (nih.gov)
  • In this paper, two sets of loblolly pine genomic sequence, 103 previously assembled BACs and 90,954 newly sequenced and assembled fosmid scaffolds, were analyzed. (plos.org)
  • Therefore, it is feasible to achieve targeted, small insertions or deletions (InDels) at user-specified loci, but more difficult to produce precise, user-defined alterations of the plant genomic sequence. (frontiersin.org)
  • Sequence comparisons across animal phyla demonstrate that AMPD has a highly conserved C-terminal catalytic domain, and a divergent N-terminal region with probable roles in isoform-specific catalytic properties, protein-protein interactions, and subunit associations [ 13 , 14 ]. (hindawi.com)
  • Full size imageThe emerging use of massively parallel sequencing (MPS) for STR profiling has revealed sequence variation within STR alleles of the same length, as well as polymorphisms (e.g. (sachecucine.it)
  • We cloned cDNAs encoding ice worm AMPD, generating a fragment encoding 543 amino acids that included a short N-terminal region and complete C-terminal catalytic domain. (hindawi.com)
  • We isolated a combined fragment 543 amino acids in length that included the complete AMPD catalytic region [ 14 ], and a 69 amino acid N-terminal region. (hindawi.com)
  • The sorts of technological advantages--advances I'm thinking about include restriction fragment length polymorphism mapping, pulsed-field gels, rapid fingerprinting strategies for physical maps, rapid sequencing. (nih.gov)
  • Cytosolic branched-chain amino acid aminotransferases are the less common of the two isoforms, found in the cytoplasm of mammalian cells almost exclusively throughout the nervous system. (wikipedia.org)
  • Human BCATc is encoded by BCAT1 Mitochondrial branched-chain amino acid aminotransferases are the more ubiquitous of the two isoforms, present in all tissues in the mitochondria of the cell. (wikipedia.org)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • Over 60% of the repetitive sequence consists of full or partial LTR (long terminal repeat) retrotransposons. (plos.org)
  • A particular feature was the high repetitive sequence content estimated to be 43.6% and that consisted mainly of transposable elements. (inrae.fr)
  • 2015).These newly discovered genetic polymorphisms could therefore increase discrimination power …SiteFinding-PCR is a method for isolating flanking sequence tags (FSTs) of T-DNA insertion lines, but the efficiency needs to be improved. (sachecucine.it)
  • The genetic map would be composed by getting DNA sequence variations, polymorphisms, variations. (nih.gov)
  • These unusually simple genomic features in the 100% complete genome sequence of C. merolae are extremely useful for further studies of eukaryotic cells. (biomedcentral.com)
  • Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. (lookformedical.com)
  • Bulked segregant RNA sequencing analysis, molecular markers and gene expression analysis indicated that the theacrine synthase (TcS) gene was the candidate gene. (bvsalud.org)
  • Cloning and sequence analysis of gene encoding plasma aquaporin of Tamarix albiflonum [J]. Bulletin of Botanical Research, 2006, 26(4): 475-479. (nefu.edu.cn)
  • Cloning and Sequence Analysis of HRD Transcription Factors Gene from Two Kinds of Ephemeral Plants [J]. Bulletin of Botanical Research, 2015, 35(2): 250-258. (nefu.edu.cn)
  • Cloning and Sequence Analysis of bHLH Gene from Taxus chinensis var. (nefu.edu.cn)
  • AJul 24, 2020 · The flanking sequence preferences derived from the random flank analysis agree well with the flanking sequence preference profiles derived from the 44-site substrate methylation data described above. (sachecucine.it)
  • We devised an original approach to the bioinformatics refinement of our sequence libraries involving important phenotypic data, based on the systematic analysis of hammerhead self-cleavage activity. (cdc.gov)
  • The predicted protein product of the beacon gene consists of 73 amino acids. (medscape.com)
  • Our present complete sequence consists of 16546747 nucleotides covering 100% of the 20 linear chromosomes from telomere to telomere, representing the simple and unique chromosomal structures of the eukaryotic cell. (biomedcentral.com)
  • Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. (sachecucine.it)
  • However, in many experiments, a non-negligible fraction of the resulting sequence reads are not homologous to the bait. (sachecucine.it)
  • Site-directed mutagenesis experiments of TCS1a, TCS1c, and TCS1h demonstrated that apart from the 225th amino acid residue, the 269th amino acid also determined the CS activity. (bvsalud.org)
  • Therefore, we aimed to complete all previous gaps and sequence all remaining chromosomal ends, and now report the first nuclear-genome sequence for any eukaryote that is 100% complete. (biomedcentral.com)
  • The global size of the 46 gaps that we have sequenced in this study is 46 469 base pairs. (biomedcentral.com)
  • The isoforms share 58% homology, but vary in location and catalytic efficiency. (wikipedia.org)
  • found that the mammalian active site contains three surfaces: surface A (Phe75, Tyr207 and Thr240), surface B (Phe30, Tyr141, and Ala314), and surface C (Tyr70, Leu153 and Val155, located on the opposite domain) that bind to the substrate in a Van der Waals-type interaction with the branched side chains of the amino acid substrates. (wikipedia.org)
  • The TcS was located in the nucleus and cytoplasm, and the theacrine can be detected in stably genetic transformed tobacco by feeding the substrate 1,3,7-trimethyluric acid. (bvsalud.org)