Antibodies recombinant fusion proteins. Medical search. Frequent questions

The following product was used in this experiment: ZO-1 Monoclonal Antibody (ZO1-1A12) from Thermo Fisher Scientific, catalog # 33-9100, RRID AB_2533147. (thermofisher.com)
Western blot was performed using Anti-ZO-1 Monoclonal Antibody (ZO1-1A12) (Product # 33-9100) and a 225 kDa band corresponding to ZO-1 was observed across along with uncharacterized band. (thermofisher.com)
Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein, or with a synthetic peptide corresponding to residues surrounding Cys60 of human Survivin. (cellsignal.com)
A novel antibody-maytansinoid conjugate, monoclonal antibody (mAb) FAP5-DM1, was developed to target a shared epitope of human, mouse, and cynomolgus monkey fibroblast activation protein-α, enabling preclinical efficacy and tolerability assessments. (aacrjournals.org)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Increasing R&D of monoclonal antibody drugs for novel clinical indications is also expected to boost growth of the market. (pharmiweb.com)
One hybridoma, producing the monoclonal antibody H7, against placental alkaline phosphatase was adopted to offer CDR-containing regions for new recombinant constructs. (projectsparadise.com)
I. High resolution epitope mapping and characterization of monoclonal antibody binding sites. (duke.edu)
The 4B4-1 monoclonal antibody specifically binds to CD137 which is also known as 4-1BB, and ILA (induced by lymphocyte activation). (bdbiosciences.com)

TNFRII antibody was raised in Mouse using recombinant soluble human TNFRII-Fc fusion protein as the immunogen. (antibodies-online.com)
Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen. (bdbiosciences.com)

Antibody recognition of the mutant proteins was determined by inhibition ELISA. (cdc.gov)
Inhibition ELISA with the mutant proteins indicated that epitope 126-131 was the dominant epitope, but mutation of epitope 120-125 was also required to eliminate mAb reactivity to Hev b 5. (cdc.gov)
Explore our vast selection of primary, secondary, and recombinant monoclonal antibodies for ELISA, Western blot, and immunohistochemistry (IHC). (sigmaaldrich.com)
This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc. (bdbiosciences.com)
The Antibody Hybridoma Core offers hybridoma, clone and subclone supernate screening by enzyme-linked immunosorbent assay (ELISA), Western blot assay and fluorescence-activated cell sorting (FACS). (mayo.edu)
However, screening by ELISA may not detect antibodies that recognize conformational epitopes of interest. (mayo.edu)
This EAAT1 antibody is for WB, ELISA. (bd-ibr.org)
This EAAT3 antibody is for WB, ELISA. (bd-ibr.org)

Although plant systems are now gaining widespread acceptance as a platform for the larger-scale production of recombinant proteins, there is still resistance to commercial uptake. (frontiersin.org)
IRIS medium is dedicated to maximal production of recombinant proteins with CHO DG44 cells. (thomassci.com)

An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)

Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. (biotium.com)
Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. (biotium.com)
For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). (biotium.com)
There are currently over 250 antibody drug conjugates (ADCs) in global clinical trials. (bioprocessintl.com)
The core uses secondary antibody conjugates specific for gamma chain (IgG) subclass isotypes, which are more desirable to obtain. (mayo.edu)
Secondary antibody conjugates specific for mu chain (IgM) subclass isotypes can also be performed upon request. (mayo.edu)

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. (biolegend.com)
The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
GT Biopharma, Inc. is an immuno-oncology biotechnology company focused on innovative treatments based on the company's proprietary Tri and Tetra-specific Natural Killer Cell Engagers (TriKEs and TetraKEs) and bispecific antibody-drug conjugate (ADC) platforms. (salesandmarketingnetwork.com)
GT's lead oncology drug candidate, OXS-1550 (DT2219) is a novel bispecific scFv recombinant fusion protein-drug conjugate composed of the variable regions of the heavy and light chains of anti-CD19 and anti-CD22 antibodies and a modified form of diphtheria toxin as its cytotoxic drug payload. (salesandmarketingnetwork.com)
BACKGROUND: In the nation-wide double-blind cluster-randomised Finnish Invasive Pneumococcal disease trial (FinIP, ClinicalTrials.gov NCT00861380, NCT00839254), we assessed the indirect impact of the 10-valent pneumococcal Haemophilus influenzae protein D conjugate vaccine (PHiD-CV10) against five pneumococcal disease syndromes. (bvsalud.org)

Instead, paired serum samples from patients, who suffered from a laboratory confirmed H1N1pdm09 infection, showed high levels or diagnostic rises (96%) in H1N1pdm virus NS1-specific antibodies and very high cross-reactivity to H3N2 subtype influenza A virus NS1 protein. (plos.org)

In this study, we characterize the linear B-cell epitopes for three monoclonal antibodies (mAbs) to Hev b 5. (cdc.gov)
Selective compounds have been developed that target either the extracellular ligand-binding region of the EGFR (including a number of monoclonal antibodies [MAbs], immunotoxins, and ligand-binding cytotoxic agents) or the intracellular tyrosine kinase region (including various small-molecule inhibitors). (medscape.com)

Novel protein to progress as priority vaccine candidate development program. (globenewswire.com)
SAN DIEGO, July 01, 2020 (GLOBE NEWSWIRE) -- Sorrento Therapeutics, Inc. (Nasdaq: SRNE, "Sorrento") announced today that it has published a pre-print publication ( https://www.biorxiv.org/content/10.1101/2020.06.29.178616v1.full.pdf ) describing initial pre-clinical results from its COVID-19 vaccination program, which introduced a novel targeted protein vaccine against COVID-19, referred to as T-VIVA-19. (globenewswire.com)
Dr. Henry Ji, Chairman and CEO of Sorrento Therapeutics, said, "If successful and approved, we plan to produce the T-VIVA-19 vaccine in our therapeutic antibody cGMP production facility in San Diego. (globenewswire.com)
Unlike other vaccine candidates, our rS1-Fc protein production could utilize our existing therapeutic antibody manufacturing processes, and we therefore believe it would be simple and easy to scale up. (globenewswire.com)
Additionally, recombinant SARS-CoV-2 Fc-fusion proteins are potential COVID-19 vaccine candidates. (invivogen.com)
The analysis was based on the presence or absence of antibody response against non-structural protein 1 (NS1) from H1N1pdm09 virus, which was not a component of Pandemrix vaccine. (plos.org)
On this research, we examined the immunogenicity of CHO-expressed recombinant SARS-CoV-2 S1-Fc fusion protein in mice, rabbits, and monkeys as a possible candidate for a COVID-19 vaccine . (genoimmun.com)
This report summarizes the fusion F protein (preF) vaccine ( 18 ). (cdc.gov)
RSVpreF (Abrysvo, Pfizer) body of evidence considered for this recommendation and is a 1-dose (0.5 mL) recombinant stabilized preF vaccine ( 19 ). (cdc.gov)

T-VIVA-19 is a recombinant fusion protein of the spike protein S1-domain and the Fc portion of the human IgG1 antibody (rS1-Fc). (globenewswire.com)

Anti-SOCS1 Antibody, clone 4H1 is an antibody against SOCS1 for use in immunoprecipitation and western blotting. (fishersci.com)
30 µg of 293 cell lysate per lane was probed with 3 µg/ml (lane 1) or 1 µg/ml (lane 2) of anti- MLH1 antibody (clone G168-728). (bdbiosciences.com)
The clone number has been updated from S57-46 to L57/46, both clone numbers name the same antibody clone. (abcam.com)

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. (biolegend.com)
Antibodies are purified by protein A and peptide affinity chromatography. (cellsignal.com)

mouse SOCS1 recombinant GST fusion protein encompassing amino acids 2-78. (fishersci.com)
We used SPOTscan analysis with overlapping octapeptides to identify the binding regions for the antibodies and then methionine substitution analysis to further define the critical amino acids (aa) in each epitope. (cdc.gov)
The function of a protein is determined by the number and sequence of amino acids, which controls the three-dimensional structure of the resulting folded polypeptide. (frontiersin.org)
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human TUSC2 (NP_009206.1). (novusbio.com)
TUSC2 is a gene that codes for a protein that is 110 amino acids long and weighs approximately 12 kDa and that functions as a tumor suppressor, which inhibits colony formation and causes G1 arrest to ultimately induce apoptosis. (novusbio.com)
Using complementary approaches, i.e. controlled enzymatic hydrolysis and production of recombinant fusion proteins, we demonstrated that, for these patients, the allergenicity of the caprine β-casein could be abolished by substituting five amino acids residues. (cea.fr)
Recombinant fusion protein containing a sequence corresponding to amino acids 30-343 of human CDCP1 (NP_835488.1). (lsbio.com)
Description: CDC37 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 378 amino acids and having a molecular mass of 44.4 kDa. (genoimmun.com)
These antibodies bind to 14 distinguishable epitopes, some separated by less than 10 amino acids. (duke.edu)
An antibody that binds between amino acids 1447 and 1467 inhibits the phosphorylation of serine residues distal to residue 1483. (duke.edu)

Recombinant SARS-CoV-2 antigens, such as the Spike (S) glycoprotein or Nucleocapsid (N), are used for the isolation of target-specific neutralizing antibodies. (invivogen.com)
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. (bvsalud.org)

Therapeutic proteins (e.g., antibodies, vaccines, enzymes, cytokines, and growth factors) account for almost half of this market, followed by industrial proteins (e.g., technical enzymes) and research reagents (e.g., antibodies for protein detection and purification) ( Markets and Markets, 2017 ). (frontiersin.org)
Focused on mammalian cell lines, our experience covers the development and manufacturing of recombinant proteins such as monoclonal antibodies, fusion proteins, cytokines and enzymes in compliance with international GMP standards (EMA/FDA). (pharmaceutical-business-review.com)

Non-structural (NS) 1 proteins from recombinant influenza A/Udorn/72 (H3N2) and influenza A/Finland/554/09 (H1N1pdm09) viruses were purified and used in Western blot analysis to determine specific antibody responses in human sera. (plos.org)
Based on quantitative Western blot analysis, only two of the 45 (4.4%) Pandemrix-vaccinated narcoleptic patients showed specific antibody response against the NS1 protein from the H1N1pdm09 virus, indicating past infection with the H1N1pdm09 virus. (plos.org)
Nine to 14 days after the fusion, hybrid supernatants are tested for the presence of the specific antibody of interest. (mayo.edu)

L19 is a fully human, clinical grade antibody that targets the alternatively spliced EDB domain of fibronectin. (oncotarget.com)
Sorrento is a clinical stage, antibody-centric, biopharmaceutical company developing new therapies to treat cancers. (globenewswire.com)
1-dose (0.5 mL) adjuvanted (AS01E) recombinant stabilized pre- shared clinical decision-making. (cdc.gov)

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)

Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Fluorescein isothiocyanate (FITC) conjugated to protein A or G binds to the fragment crystallizable region of IgG immunoglobulins, especially IgG2a. (mayo.edu)
the target protein binds to the column, and unwanted proteins are eluted. (the-scientist.com)

While some researchers have fused the IL2 moiety at the C-terminal extremity of the heavy chain or of the light chain of antibodies in full IgG format, these Oncotarget authors have preferred to use antibody fragments, as they may exhibit more favorable tumor: organ ratios and as those products may clear more rapidly from circulation, thus avoiding prolonged cytokine-related toxicity. (oncotarget.com)
Both single-chain variable fragments (scFv) antibodies or divalent tandem monospecific scFv [sc(Fv)2] antibodies with properties suitable for targeting were produced. (projectsparadise.com)

The masking of IL2 epitopes using antibodies allowed the selective activation of lymphocytes sub-populations, improving immune-stimulatory activity. (oncotarget.com)
Antibodies with adjacent epitopes can compete with each other at linear distances up to 5 or 6 nm, and many antibodies that bind 3-7-nm apart can enhance the binding of each other to myosin. (duke.edu)

This novel, catalytic-type mechanism of action is called "event-driven" pharmacology, which contrasts with "occupancy-driven" pharmacology, whereby the function of a target protein is directly blocked by a small-molecule inhibitor. (chempartner.com)

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. (caslab.com)
From monoclonal antibodies and recombinant proteins to the most advanced cell and gene therapies, our comprehensive set of products and services will accelerate your workflows, reduce risk, and get you to the finish line faster. (sigmaaldrich.com)
EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. (thermofisher.com)
This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. (prosci-inc.com)
Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
The protein encoded by this gene is a member of the interleukin 1 cytokine family. (genetex.com)
The roles of b-catenin are 'classically' defined: as an adhesion protein and as a signaling protein, transducing extracellular signals to the nucleus to modify gene expression. (chemdiv.com)
The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)

Oncotarget recently published " A novel format for recombinant antibody-interleukin-2 fusion proteins exhibits superior tumor-targeting properties in vivo which reported that here, the authors describe four novel formats for the L19-IL2 fusion, featuring different arrangements of antibody and IL2. (oncotarget.com)
A comparative quantitative biodistribution analysis in tumor-bearing mice using radioiodinated proteins revealed that the novel format exhibited the best biodistribution results. (oncotarget.com)
CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. (biolegend.com)
Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)

The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. (caslab.com)
InvivoGen offers a collection of pFUSE-Fc plasmids developed to facilitate the expression and production of Fc-Fusion proteins, by fusing a sequence encoding a given protein to the Fc region of an immunoglobulin (Ig). (invivogen.com)
Then the DNA for some or all of the variable portion of the mouse antibody is merged with DNA for human immunoglobulin. (msdmanuals.com)
Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)

Proteins are therefore molecules of great complexity and near infinite diversity, making them suitable for many different applications. (frontiersin.org)
Fc-fusion proteins are molecules consisting of an IgG-Fc domain fused to a protein of interest, such as an extracellular domain of a receptor, ligand, enzyme, or peptide. (invivogen.com)
We used a series of COOH-terminally deleted recombinant myosin molecules to map precisely the binding sites of 22 monoclonal antibodies along the tail of Acanthamoeba myosin-II. (duke.edu)

The following product was used in this experiment: eIF2 beta Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-89868, RRID AB_2805811. (thermofisher.com)

This antibody was developed for use in flow cytometry. (bdbiosciences.com)

The SignalStain ® Proliferation/Apoptosis IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect cellular apoptosis or proliferation. (cellsignal.com)
β-Catenin is a fascinating protein with many important cellular and developmental functions. (chemdiv.com)
β -catenin has many binding partners that mediate a diverse set of cellular functions, and the protein probably acts as a 'hub' on which many cellular signaling networks impinge. (chemdiv.com)
The ubiquitin-mediated destruction of cellular proteins plays a crucial part in many cellular functions by altering the cellular protein concentration. (chempartner.com)

For instance, protein conjugation and derivatization methods, including albumin-fusion15, PEGylation and Fc-fusion are in trend for increasing the circulating half-life of a therapy. (pharmiweb.com)

Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
The Western blot is a technique used to identify candidate hybrids, clones, subclones and monoclonal antibodies of interest that recognize and bind to one or more specific molecular weight proteins of interest. (mayo.edu)
Proteins are run in an SDS-polyacrylamide gel electrophoresis (SDS-PAGE) apparatus, with proteins migrating according to molecular weight. (mayo.edu)
The molecular weight of proteins were 102, 88 and 80 kDa, so they were named α, β and γ-catenin. (chemdiv.com)

Also included in the kit are control slides that can be used to verify the performance of each antibody and a primary antibody diluent. (cellsignal.com)

The protein-bound membrane is then incubated with a known or test antibody reagent, followed by a secondary antibody conjugated with a color indicator enzyme or substrate (or radiolabeled isotope). (mayo.edu)

Recombinant Therapeutic Antibodies and Proteins Market Sh. (pharmiweb.com)
Therapeutic proteins such as non-covalent binders are developed in labs and find application in the pharmaceutical sector. (pharmiweb.com)
Therapeutic proteins are used in the treatment of chronic and rare disorders. (pharmiweb.com)
These proteins support a targeted therapeutic process and reimburse the lack of a pivotal protein in order to offer a tailored method of therapy. (pharmiweb.com)
For instance, according to the National Center for Biotechnology Information (NCBI) data of 2017, the U.S. Food and Drug Administration (FDA) approved over 140 recombinant therapeutic proteins for human use and several hundred are currently in development. (pharmiweb.com)
The global recombinant therapeutic antibodies and proteins market size was valued at US$ 91.2 billion in 2017, and is expected to witness a robust CAGR of 12.2% over the forecast period (2018 - 2026). (pharmiweb.com)
Use of several recombinant methodologies has enhanced the half-life, functionality, and targeting ability of new therapeutic protein drugs. (pharmiweb.com)
Some of the widely adopted recombinant therapeutic products are monoclonal antibodies that can be produced using various platform-based approaches. (pharmiweb.com)
Major governments around the world are focused on supporting R&D in recombinant therapeutic antibodies and proteins, which is expected to boost growth of the market. (pharmiweb.com)
High prevalence of rare disorders is also contributing to growth of the recombinant therapeutic antibodies and proteins market. (pharmiweb.com)
The market growth is restricted by stringent regulations that govern the performance and safety of recombinant therapeutic drugs. (pharmiweb.com)
The Helicobacter pylori Neutrophil Activating Protein (HP-NAP) is endowed with immunomodulatory properties that make it a potential candidate for anticancer therapeutic applications. (bvsalud.org)

Description: A polyclonal antibody for detection of EAAT1 from Human. (bd-ibr.org)

More than 300 protein-based medicines have been approved in the USA and Europe, and proteins account for almost a third of all pharmaceuticals in development ( Walsh, 2018 ). (frontiersin.org)

Market growth has been supported by advances in recombinant protein production technologies, including the engineering of expression hosts, the optimization of upstream cultivation (e.g., bioreactor design, nutritional, and physical parameters), and the development of more efficient protein extraction and purification methods. (frontiersin.org)
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)

Each antibody is validated for use in immunohistochemical assays using multiple approaches. (cellsignal.com)
This product is intended to be used as a blocking antigen for antibody competition assays. (novusbio.com)

The Antibody Hybridoma Core utilizes the fusion partner cell line FOX-NY, a nonimmunoglobulin-secreting myeloma cell line that is hypoxanthine-aminopterin-thymidine (HAT) sensitive. (mayo.edu)
Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)

Pegylated forms of IL2 have shown superior properties in mouse models of cancer and encouraging results in patients as single agents or in combination with anti-PD-1 antibodies. (oncotarget.com)
Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. (prosci-inc.com)
IHC-P analysis of mouse lung tissue using GTX55675 IL1 beta antibody. (genetex.com)
WB analysis of mouse liver tissue lysate using GTX55675 IL1 beta antibody. (genetex.com)
Immunohistochemical analysis of paraffin embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Phospho-Histone H3 (Ser10) Antibody, PCNA (PC10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody and Survivin (71G4B7E) Rabbit mAb. (cellsignal.com)
Two different monoclonal antibodies were used to immunoprecipitate MLH1 from equal amounts of whole cell extracts of NIH/3T3 mouse cells. (bdbiosciences.com)
The G168-728 antibody recognizes human and mouse MLH1 (80-85 kDa). (bdbiosciences.com)
However, mice that are not immunized by the core are required to be certified as "mouse virus-free" prior to fusion. (mayo.edu)
Description: A Rabbit Polyclonal antibody against EAAT3 from Human/Mouse/Rat. (bd-ibr.org)
To minimize the problems due to use of pure mouse antibody, researchers have used recombinant DNA techniques to create monoclonal antibodies that are part human and part mouse. (msdmanuals.com)

Rentschler delivers upstream process (USP) development for cell cultivation and downstream process (DSP) development for protein purification as well as formulation development. (pharmaceutical-business-review.com)

KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM) , while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. (caslab.com)
ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-85561. (novusbio.com)

Over the last three decades, the expression of recombinant proteins in plants and plant cells has been promoted as an alternative cost-effective production platform. (frontiersin.org)
Here, we discuss the strengths of plant expression systems for specific applications, but mainly address the bottlenecks that must be overcome before plants can compete with conventional systems, enabling the future commercial utilization of plants for the production of valuable proteins. (frontiersin.org)
Even so, plants as an alternative expression platform offer unique advantages, particularly when target proteins are difficult to produce in conventional systems, require specific qualitative properties such as particular glycan profiles, and/or must be produced on a larger scale in response to urgent demand. (frontiersin.org)
IRIS is suitable for recombinant protein expression for human like glycosylation, for example Erythropoietin, antibodies, Fc-fusion proteins. (thomassci.com)
Canton Biologics cell line development platform for protein expression. (maedbook.com)

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3, or with a synthetic peptide surrounding amino-terminal residues adjacent to Asp175 of human caspase-3. (cellsignal.com)

Approximately 80% of the mice's sera possessed neutralizing antibodies and completely prevented virus infection in in vitro cell cultures using 100 TCID50 viruses and VERO cells. (globenewswire.com)
a recombinant protein synthesized from in vitro methods may also be used. (mayo.edu)

On 5 December 2008, orphan designation (EU/3/08/592) was granted by the European Commission to Medimmune Limited, United Kingdom, for murine anti-CD22 antibody variable region fused to truncated Pseudomonas exotoxin 38 for the treatment of hairy cell leukaemia. (wikipedia.org)
Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)

CD137 is a 39 kD transmembrane protein also known as 4-1BB. (biolegend.com)

The antibody was conjugated with BD Horizon BV480 under optimal conditions that minimize unconjugated dye and antibody. (bdbiosciences.com)

The drug consists of the binding fragment (Fv) of an anti-CD22 antibody fused to a toxin called PE38. (wikipedia.org)

PROTACs are being developed more and more for many kinds of target proteins, even for "undruggable targets. (chempartner.com)

In this dissertation, the goal was to enhance our knowledge on how new recombinant antibodies might be tailored to optimize localization to experimental tumors in mice. (projectsparadise.com)

Anatomy17

Organisms20

Diseases2

Chemicals and Drugs87

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Phenomena and Processes58

Disciplines and Occupations1

Information Science3

Health Care3

Monoclonal Antibody9

Immunogen2

ELISA8

Production of recombinant proteins2

Isotype1

Conjugates7

Conjugate5

Reactivity1

MAbs2

Vaccine9

IgG11

Clone3

Affinity chromatography2

Amino acids10

Antigens2

Cytokines2

Specific antibody3

Clinical3

DataSheet1

Soluble1

Binds3

Fragments2

Epitopes2

Small-molecule1

Gene8

Tumor4

Immunoglobulin4

Molecules3

Thermo Fisher Sci1

Flow Cytometry1

Cellular4

Conjugation1

Molecular4

Primary antibody1

Secondary1

Therapeutic12

Detection1

20181

Purification methods2

Assays2

Hybridoma3

Mouse11

Formulation1

Cytoplasmic2

Specificity1

Expression5

Synthetic peptide1

Vitro2

Murine2

Known as 4-1BB1

Optimal1

Toxin1

Target proteins1

Localization1