Antibodies histocompatibility antigens. Medical search. Frequent questions

Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. (bdbiosciences.com)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human HLA-DR Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB4869N) or (B) isotype control antibody (Catalog # IC002N ) Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P ). (rndsystems.com)
To test this hypothesis, both an Ag‐specific mouse B cell clone CH12.LX, and freshly isolated resting splenic B cells were cultured with either soluble or membrane‐bound forms of the T cell ligand for CD40 (CD40L), in the presence or absence of additional signals provided by Ag or anti‐IgM, interleukin‐4, and class II‐specific monoclonal antibody (mAb). (uthscsa.edu)
Classical " CARs consist of an extracellular binding domain mostly derived from a monoclonal antibody fragment (single-chain variable fragment-scFv), which is linked to intracellular binding domains of the T-cell receptor complex. (nature.com)
The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. (exbio.cz)
The following product was used in this experiment: MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), NovaFluor™ Blue 610-70S, eBioscience™ from Thermo Fisher Scientific, catalog # M024T02B06, RRID AB_2910831. (thermofisher.com)
Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). (thermofisher.com)

Abnormal expression of major histocompatibility complex class II molecules in the placenta may trigger the initiating immunologic event in this organ, which is followed by cross-reactivity with an antigen present in the skin and the characteristic cutaneous signs of the disease. (medscape.com)
simultaneously bind to major histocompatibility complex published a study of seven patients with severe streptococ- class II molecules and T-cell receptor molecules bearing a cal infections: SPE-A was detected in serum samples from four patients (27). (cdc.gov)
The TCR-CD3 complex is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. (ptglab.com)
Like the HLA system, MICA molecules can elicit antibody responses during pregnancy, blood product transfusion, or organ transplant rejection. (loinc.org)
Several environmental, genetic, and immune factors create a "perfect storm" for the development of coeliac disease: the antigen gluten, the strong association of coeliac disease with HLA, the deamidation of gluten peptides by the enzyme transglutaminase 2 (TG2) generating peptides that bind strongly to the predisposing HLA-DQ2 or HLA-DQ8 molecules, and the ensuing unrestrained T cell response. (frontiersin.org)
The present study was designed to test the hypothesis that signals delivered via the B cell antigen (Ag) receptor (membrane immunoglobulin, mIg) and major histocompatibility complex (MHC) class II molecules enhance B cell responsiveness to CD40‐mediated signals, providing specificity to the Ag‐nonspecific, MHC‐unrestricted CD40 signal. (uthscsa.edu)
Bishop, GA, Warren, WD & Berton, MT 1995, ' Signaling via major histocompatibility complex class II molecules and antigen receptors enhances the B cell response to gp39/CD40 ligand ', European Journal of Immunology , vol. 25, no. 5, pp. 1230-1238. (uthscsa.edu)
The addition of separate adapter molecules (AMs) specific for tumor antigens and CAR-immune cells targeting these AMs allows a more precise and temporally limited therapy. (nature.com)
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. (thermofisher.com)
Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. (thermofisher.com)
Initiation and regulation of immune responses in humans involves recognition of peptides presented by human leukocyte antigen class II (HLA-II) molecules. (biomedcentral.com)
Antigen variation in pathogens and tumor antigens, and extensive polymorphism of HLA molecules increase the number of targets for screening studies. (biomedcentral.com)
Full overlapping studies of four antigens identified binding affinity of 103 peptides to seven common HLA-DR molecules (DRB1*0101, 0301, 0401, 0701, 1101, 1301, and 1501). (biomedcentral.com)
CTLs recognize peptide antigens presented by major histocompatibility complex class I (MHC-I) molecules on infected cells or cancer cells and kill them. (biomedcentral.com)
B cells produce antibodies that specifically recognize pathogen- or cancer related molecules. (biomedcentral.com)
MHC-II molecules present antigenic peptides internalized by professional antigen presenting cells, such as macrophages, dendritic cells, or T lymphocytes. (biomedcentral.com)
Antigens may also exist on their own-for example, as food molecules or pollen. (msdmanuals.com)
Antigens are the molecules that are recognized and stimulate the cells of immune system. (medscape.com)
Immunoglobulins (Igs), the term is sometimes used interchangeably with "antibodies," are glycoprotein molecules produced by B lymphocytes and plasma cells in response to an immunogen or after recognition of specific epitopes on the antigen. (medscape.com)
Variable region constitutes the antibody binding region of the molecule to the different antigens as it consists of about 110 amino acids that vary widely among the different antibody molecules. (medscape.com)

Alternatively, T cells have been gene-modified with a TCR-like antibody or chimeric antigen receptor (CAR). (fujita-hu.ac.jp)
Besides the secretion of pro-inflammatory cytokines (TNF-alpha, IL-1beta, and IL-6), the increased expression of receptor repertoire (pattern recognition receptors, PRR) on APCs, mostly macrophages and dendritic cells, occurs as a result of the release of endogenous and exogenous antigens (damage-associated molecular patterns, DAMPs, and pathogen-associated molecular patterns, PAMPs). (hindawi.com)
CD4 is an accessory protein for major histocompatibility complex (MHC) class-II antigen/T-cell receptor interaction. (ptglab.com)
Demonstration of heavy and light chain antigenic determinants on the cell-bound receptor for antigen. (wikidata.org)
Chimeric antigen receptor (CAR)-T cell therapies are on the verge of becoming powerful immunotherapeutic tools for combating hematological diseases confronted with pressing medical needs. (nature.com)
Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments. (exbio.cz)
Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. (exbio.cz)
Rejection involves a complex set of T-cell receptor mediated pathways that lead to cytotoxic activity, B-cell memory and antibody formation, and cell death of the transplant allograft. (canadiem.org)
In response to environmental challenges, FOXP3 transcription and splicing can be finely regulated by T cell antigen receptor stimulation, glycolysis, fatty acid oxidation, and reactive oxygen species, with various signaling pathways involved. (bvsalud.org)

The genetic transfer of T-cell receptors (TCRs) directed toward target antigens into T lymphocytes has been used to generate antitumor T cells efficiently without the need for the in vitro induction and expansion of T cells with cognate specificity. (fujita-hu.ac.jp)
These data implicate that T cells with high-affinity antigen receptors reduce the ability to lyse targets with low-density peptide/MHC complexes (∼100 per cell), while they could respond at cytokine production level. (fujita-hu.ac.jp)
It is an autoimmune disorder, in which weakness is caused by circulating antibodies that block acetylcholine receptors at the post-synaptic neuromuscular junction, [1] inhibiting the stimulative effect of the neurotransmitter acetylcholine. (doctorbhatia.com)
Some forms of the antibody impair the ability of acetylcholine to bind to receptors. (doctorbhatia.com)
One way to achieve this is to genetically modify immune cells, mainly T cells and recently also natural killer (NK) cells, to express chimeric antigen receptors (CARs). (nature.com)

We show that immunization of HLA-A2 transgenic mice with tetramerized recombinant HLA-A2 incorporating HA-1 H minor histocompatibility antigen (mHag) peptides and β2- microglobulin (HA-1 H/HLA-A2) generate highly specific antibodies. (fujita-hu.ac.jp)
In addition, CeD patients produce highly disease-specific antibodies against deamidated gluten peptides and the enzyme tissue transglutaminase 2 (TG2) ( 2 - 4 ). (frontiersin.org)
This antibody was developed against a mixture of synthetic peptides containing amino acids 180-196, 353-370, and 473-489 of human TLR2 (NP_003255). (novusbio.com)
Antigens are peptides or recombinant or native dependent on the production method. (histoready.com)
HLA-II peptide binding studies involve multiple overlapping peptides spanning individual antigens, as well as complete viral proteomes. (biomedcentral.com)

CD205 is responsible for uptake and processing of captured antigens for dendritic cells. (exbio.cz)

100-fold higher antigen density to exert cytotoxicity compared with the cognate CTL clone, they could produce inflammatory cytokines against cells expressing HLA-A2 and HA-1 H transgenes. (fujita-hu.ac.jp)
The histocompatibility differences between the donor and the recipient, the presence of donor's immunocompetent cells, and the inability of the recipient to reject these cells were defined as the basic pathogenic prerequisites for GVHD development by Billingham in 1966 [ 10 ]. (hindawi.com)
Cytotoxic T lymphocytes were determined as the cellular effectors of GVHD, and the key role of antigen-presenting cells (APCs) in T-lymphocyte activation was established during the following years [ 11 , 12 ]. (hindawi.com)
Anti-HLA-DR is intended for in vitro diagnostic use in the identification of cells expressing the HLA-DR antigen, using a BD FACS™ brand flow cytometer. (bdbiosciences.com)
When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)
This binding results in the activation have been implicated with streptococcal toxic shock syn- of a large proportion of antigen-presenting cells and T drome (STSS). (cdc.gov)
Once the graft has escaped the initial acute phase rejection reactions, a cumulative unresponsiveness to the graft develops as the recipient is continually exposed to donor MHC, a stable state that sometimes depends on the development of antigen-specific T-suppressor cells [ 371 , 5349 - 5354 ]. (nanomedicine.com)
Here, we show that even expression of minor histocompatibility (mH) antigens is sufficient to provoke acute rejection of tissues differentiated from ES cells. (ox.ac.uk)
This propensity for tolerance could be attributed to the paucity of professional antigen-presenting cells and the expression of transforming growth factor (TGF)-?2. (ox.ac.uk)
Separation of normal and immune lymphoid cells by antigen-coated coated columns. (wikidata.org)
The roles of the M cells include absorption, transport, processing, and presentation of antigens to subepithelial lymphoid cells. (medscape.com)
the latter are antigen-presenting cells (APCs) that function as memory cells interacting with type 1 THCs. (medscape.com)
M cells in the intestinal epithelium overlying Peyer patches allow transport of antigens to the lymphoid tissue beneath it. (medscape.com)
In fact, as antigen-presenting cells (APC), microglia are endowed with the ability to release pro-inflammatory cytokines and chemokines into the CNS tissue microenvironment. (atlasantibodies.com)
CAR-expression on T or NK cells allows them to specifically target cancer cells via recognition of tumor associated antigens. (nature.com)
Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. (thermofisher.com)
The goal of vaccination is to induce immunity against pathogens and cancer cells by stimulating antigen-specific cytotoxic T lymphocytes (CTLs) or B cells. (biomedcentral.com)
Two monoclonal antibodies, TAL-1B5 and TAL-3C3, specific for human Ia alpha-chain subunits have been produced by fusing P3/NSI/1-Ag4-1 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified alpha-chains. (ox.ac.uk)
2D-NEPHGE/SDS-PAGE analysis of TAL-1B5 immunoprecipitates from [35S]-methionine biosynthetically labelled cells revealed the presence of a number of alpha-chain spots in association with beta-chain products of three previously described loci (beta-1, beta-2, beta-3) suggesting that this antibody recognizes an antigenic site common to those human Ia alpha-chains so far identified. (ox.ac.uk)
The cellular response is mainly a lymphocyte-mediated reaction, whereas the humoral response includes production of antibodies against the antigen by the plasma cells. (medscape.com)
Human leukocyte antigens (HLAs) are proteins that help the body's immune system tell the difference between its own cells and foreign, harmful substances. (medlineplus.gov)
In ITP, for example, his laboratory is interested in how platelet antigens are processed and presented by antigen-presenting cells to activate T lymphocytes. (lu.se)

This interaction is central to antigen specificity in the adaptive immune response. (rndsystems.com)

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD205 (HD30) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human HLA-DR (MEM-12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Separation of human monocytes (red-filled) from CD205 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD205 (HD30) PE antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Antibodies can be found on the surface of lymphocytes as an integral part of the cell membrane protein or can be freely circulating in the blood or be part of one of the body's gland secretion. (medscape.com)

Competitive inhibition studies demonstrated that both monoclonal antibodies recognize the same or spatially related alpha-chain antigenic determinants with some slight cross-reactivity against beta-chains. (ox.ac.uk)

Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. (exbio.cz)
Epitopes are a component of the antigen that are recognized by the immune system and determine whether the cellular or the humoral arm of the immune system shall be activated against that particular antigen. (medscape.com)

Kidney transplant candidates with preformed, donor-specific antibodies may undergo a pretransplant desensitizing protocol. (medscape.com)

The most general pre-nanomedical method to suppress immune system acute responsiveness is called antigen nonspecific immunosuppression. (nanomedicine.com)
Since the immune response to antigen requires clonal proliferation, agents that block mitosis are effective inhibitors of the immune response. (nanomedicine.com)
HLA-B27 and the immune response to enterobacterial antigens in ankylosing spondylitis. (anthonynolan.org)
Besides modification based on a second-generation CAR, more advanced CAR-immune cell therapeutics are being tested, which utilize precise insertion of genes to circumvent graft-versus-host disease (GvHD) or employ a dual targeting approach and adapter CARs in order to avoid therapy resistance caused by antigen loss. (nature.com)
Furthermore, the so-called adapter CARs have been developed by splitting antigen recognition and CAR-immune cell activation. (nature.com)
The purified BDC elicited strong allogeneic mixed leukocyte reactions and HLA classes II- and I-restricted antigen-specific primary immune responses. (nih.gov)
Involved in the presentation of foreign antigens to the immune system. (abcam.co.jp)
Antigens are any substances that the immune system can recognize and that can thus stimulate an immune response. (msdmanuals.com)
If antigens are perceived as dangerous (for example, if they can cause disease), they can stimulate an immune response in the body. (msdmanuals.com)
The body's immune system can react adaptively against the antigen via 2 pathways: cellular or humoral. (medscape.com)
In this immune reaction, antibody immunosuppressive, immunostimulatory, and autoim- is directed against a person's own cellular surface anti- mune. (cdc.gov)
His research is aimed at understanding the cellular immune mechanisms responsible for the generation of IgG antibodies that recognize platelet antigens. (lu.se)
These antibodies can lead to clinically significant adverse effects such as autoimmunity causing immune thrombocytopenia (ITP) and alloimmunity causing platelet transfusion refractoriness. (lu.se)

Leucocyte Typing IV: White Cell Differentiation Antigens. (bdbiosciences.com)

Human leukocyte antigen: the major histocompatibility complex of humans. (medlineplus.gov)

One single-chain variable region moiety (scFv) antibody, #131, demonstrated high affinity (K D =14.9 nM) for the HA-1 H/HLA-A2 complex. (fujita-hu.ac.jp)
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. (biolegend.com)

Serum samples taken on day 9 from neutralizing anti-SAg antibodies appears to be a key risk this patient showed evidence of seroconversion with high factor for the development of staphylococcal and strepto- levels of anti-SMEZ antibodies that neutralized SMEZ-1 and 12 other SMEZ-variants. (cdc.gov)
In this article, the term "acute-phase serum" refers to antigens can also cause toxic shock (11). (cdc.gov)

The histochemical demonstration of acid phosphatase (ACP) and adenosine triphosphatase (ATP) has been combined with standard immunofluorescence techniques, using a panel of monoclonal and conventional antibodies, to examine lymphocyte and macrophage subsets and their microanatomical relationships within the subcutaneous rheumatoid nodule (RN). (nih.gov)

Major histocompatibility complex class I chain-related antigen A (MICA) proteins are like HLA class I gene products. (loinc.org)
Myasthenia gravis is an autoimmune disease: it features antibodies directed against the body's own proteins. (doctorbhatia.com)
Progress in different areas of basic research, new animal models, and the generation of specific antibody markers to target essential proteins help find and improve the treatment of patients with neuroimmunological diseases. (atlasantibodies.com)

Nevertheless, despite their immunogenicity in vivo, transplantation tolerance may be readily established by using minimal host conditioning with nondepleting monoclonal antibodies specific for the T cell coreceptors, CD4 and CD8. (ox.ac.uk)
If successful, this protocol reduces antibody levels to the point where renal transplantation becomes feasible. (medscape.com)

A role for the involvement of major histocompatibility complex class II antigens seems likely because of the association with human leukocyte antigens DR3 and DR4. (medscape.com)
Major histocompatibility complex class III associations have also been observed, as has a high frequency of anti-human leukocyte antigen antibodies. (medscape.com)
CeD is triggered by gluten consumption in genetically susceptible individuals carrying certain major histocompatibility complex (MHC) class II human leukocyte antigen (HLA) variants ( 5 , 6 ). (frontiersin.org)
The protein is called human leukocyte antigen B27 (HLA-B27). (medlineplus.gov)

Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression. (bdbiosciences.com)
Antibodies belong to immunoglobulin family of protein and have 5 isotypes (G, A, M, E, D) with further subclasses of G and A. The classes are differentiated with the characteristics of the Ig heavy chain of the antibody. (medscape.com)

Antigen nonspecific methods include the use of cytotoxic drugs that interfere with all cell division in the body [ 383 ]. (nanomedicine.com)
Binding of a tumor antigen via the scFv activates the T cell in a major histocompatibility-independent manner which leads to a cytotoxic response [ 3 ]. (nature.com)

Immunohistochemistry-Paraffin: TLR2 Antibody [NB100-56720] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml. (novusbio.com)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.co.jp)

A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. (bdbiosciences.com)

No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. (bdbiosciences.com)

Prednisone and prednisolone [ 386 ] act powerfully to suppress the inflammation accompanying a rejection crisis, and also appear to reduce the expression of class II histocompatibility antigens, thus reducing the immunogenicity of the transplant. (nanomedicine.com)
The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long. (thermofisher.com)

and that tightly bind to the antigen of an invader, tagging the invader for attack or directly neutralizing it. (msdmanuals.com)
The antibodies then specifically bind to only those particular antigens. (medscape.com)

Left Plot) or BD Horizon™ RB780 Mouse Anti-Human HLA-DR antibody (Cat. (bdbiosciences.com)
HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. (rndsystems.com)
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD205 (HD30) PE antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Production and characterization of monoclonal antibodies recognizing the alpha-chain subunits of human ia alloantigens. (ox.ac.uk)

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry. (thermofisher.com)

Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. (exbio.cz)

Recipients of kidney transplants undergo an extensive immunologic evaluation that primarily serves to avoid transplants that are at risk for antibody-mediated hyperacute rejection. (medscape.com)

Western Blot: TLR2 Antibody [NB100-56720] - Analysis using the Azide Free version of NB100-56720. (novusbio.com)

T cell recognition of cell surface antigens. (wikidata.org)

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)
Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Tested: This M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)

Thereby, several antigens can be targeted at once and the therapy can be adapted in case antigen-loss tumor variants appear. (nature.com)

Detection of TLR2 in transfected cell lysate using this antibody. (novusbio.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)

When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. (bdbiosciences.com)

Anatomy29

Organisms14

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Chemicals and Drugs115

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Phenomena and Processes49

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Monoclonal antibody8

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Receptor9

Receptors5

Peptides5

Dendritic cells1

Cells22

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Immune13

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