AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
Anaplasma marginaleAnaplasmaAnaplasmosisAnaplasma phagocytophilumDermacentorAnaplasma centraleTicksCattle DiseasesBacterial Outer Membrane ProteinsEhrlichiosisCattleArachnid VectorsTick-Borne DiseasesRhipicephalusAntigens, BacterialAnaplasma ovisArthropod VectorsAnaplasmataceaeEhrlichiaSalivary GlandsEhrlichia ruminantiumBacterial VaccinesBabesia bovisIxodesBabesiaAntigenic VariationAntibodies, BacterialBabesiosisMolecular Sequence DataRickettsial VaccinesBacterial ProteinsTick InfestationsTheileriasisErythrocytesBacteremiaDNA, BacterialAnaplasmataceae InfectionsAmino Acid SequenceRNA, Ribosomal, 16SSequence Analysis, DNAImmunodominant EpitopesPolymerase Chain ReactionBase Sequence
PhagocytophilumCattleRickettsiaAnaplasmosisBabesiaInfectionPrevalencePlatysTicksMolecularStrainsBovinosCharacterizationSequenceGenesGeneTickStrainSamplesPositiveCellsAnaplasmosisPathogensBovisBacteriaBigeminaCentraleEhrlichiaSpeciesSusceptibleGenBankOuter membraneCalvesFloridaHumansSignificantlyPresenceIdentificationBloodSurface
Phagocytophilum5
  • These pathogens include Anaplasma marginale and A. phagocytophilum, which cause bovine and granulocytic anaplasmosis, respectively. (usda.gov)
  • Objective -To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale , with and without tick salivary extract, in cattle. (avma.org)
  • Conclusions and Clinical Relevance -Results indicated an important biological interaction between A marginale and A phagocytophilum infection as well as with tick saliva in disease kinetics and severity in cattle, which may be important for interpretation of diagnostic tests and management of disease in areas where both pathogens occur. (avma.org)
  • Romanowsky stained canine blood smear containing morulae (arrow) of Anaplasma phagocytophilum within a neutrophil. (ncvetp.org)
  • Infections with Anaplasma phagocytophilum are most common in the northeastern United States, in the upper Midwest, and along the West Coast. (ncvetp.org)
Cattle12
  • To detect Anaplasma marginale among carrier cattle by using polymerase chain reaction (PCR) technique, 64 blood samples, fromhealthy cows in abattoir of Al- Nasiriyah city were collected from June till August, 2017 in this study. (vetmedmosul.com)
  • Molecular study of Anaplasma marginale parasite in carrier cattle in Al-Nasiriyah city', Iraqi Journal of Veterinary Sciences , 32(2), pp. 299-301. (vetmedmosul.com)
  • Anaplasma marginale is the most prevalent tick -borne haemoparasite of cattle and causes huge economic losses to the dairy industry worldwide. (bvsalud.org)
  • It has never been reported that sheep , goats , and cattle in Tank , Ghulam Khan, Birmil and Miran Shah areas were infected with A. marginale. (bvsalud.org)
  • All samples of blood and ticks were collected through random sampling from March 2021 to January 2022 from cattle , sheep and goats and screened through PCR for anaplasmosis by using primer pairs of Anaplasma spp. (bvsalud.org)
  • PCR results for A marginale were positive in spleen, lung, lymph node, heart, and ear skin of infected cattle. (avma.org)
  • Bovine anaplasmosis, caused by the blood-borne parasite Anaplasma marginale, is a worldwide problem for cattle. (beefmagazine.com)
  • Molecular detection and characterization of Babesia bovis, Babesia bigemina, Theileria species and Anaplasma marginale isolated from cattle in Kenya. (obihiro.ac.jp)
  • We are using a combination of A. marginale strains, some of which we recently isolated from Kansas cattle herds, to help determine how strains differ in their susceptibility to tetracycline antimicrobials, specifically chlortetracycline, the most common antimicrobial used to control anaplasmosis. (k-state.edu)
  • Abstract Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. (edu.vn)
  • His Beef cattle research is multidisciplinary, incorporating elements of Trypanosoma vivax, Herd and Anaplasma marginale. (research.com)
  • Infectious blood disease in cattle caused by the rickesttsial parasites Anaplasma marginale and Anaplasma centrale. (sanfovet.com)
Rickettsia4
  • Major surface protein 2 (MSP2) is an immunodominant and antigenically variant protein in the outer membrane of the rickettsia Anaplasma marginale. (oregonstate.edu)
  • Several pathogens including tick-borne pathogens ( Borrelia/Borreliella , Anaplasma , Neoehrlichia , Rickettsia ) and opportunistic bacteria ( Williamsia ) were transmitted to the skin microbiome and some of them disseminated to the blood or spleen of the mice. (biomedcentral.com)
  • Title : Rickettsia and Anaplasma species in Dermacentor andersoni ticks from Washington Personal Author(s) : Francis, Lily;Paddock, Christopher D.;Dykstra, Elizabeth A.;Karpathy, Sandor E. (cdc.gov)
  • First Molecular Evidence of Anaplasma ovis and Rickettsia spp. (google.com.pa)
Anaplasmosis6
  • The most sensitive method for the diagnosis of anaplasmosis is the method of polymerase chain reaction, DNA extraction was performed only on 24 blood samples which were positive for Anaplasma spp. (academicjournals.org)
  • Using Anaplasma marginale, which causes bovine anaplasmosis, and Dermacentor andersoni tick cells, we determine that iron is required for pathogen replication in tick cells. (usda.gov)
  • Anaplasmosis is a tick-borne disease caused by an obligate gram-negative bacteria, Anaplasma (A.) marginale. (lutzonilab.net)
  • Kathryn Reif, assistant professor in the diagnostic medicine and pathobiology department in the Kansas State University College of Veterinary Medicine, is leading a project that focuses on optimizing antimicrobial use to control active infection of the hemoparasitic pathogen, Anaplasma marginale, the causative agent of bovine anaplasmosis. (k-state.edu)
  • Anaplasmosis is caused by the bacterium Anaplasma marginale. (dtnpf.com)
  • Control of active infection of anaplasmosis caused by Anaplasma marginale susceptible to chlortetracycline. (pharmgate.com)
Babesia2
  • Association between chronic Anaplasma marginale and Babesia spp. (bvsalud.org)
  • infection and hematological parameters of taurine heifers / Associação entre infecção crônica por Anaplasma marginale e Babesia spp. (bvsalud.org)
Infection5
  • This study aimed to determine the occurrence of A. marginale infection in blood and tick samples collected from livestock animals in the districts located in Khyber Pakhtunkhwa (KPK), Pakistan . (bvsalud.org)
  • We report the first PCR based detection of A. marginale infection in blood samples and in R. sanguineus ticks of goats simultaneously. (bvsalud.org)
  • Anemia occurred 25 to 32 days after A marginale infection, which was attenuated by tick saliva. (avma.org)
  • The ophthalmic findings and the presence of Anaplasma marginale corpuscles in parasited red blood cells indicated that the systemic a infection may have induced the chronic uveitis. (vin.com)
  • The authors conclude that the A. marginale systemic infection was the primary cause of the chronic uveitis. (vin.com)
Prevalence1
Platys1
  • Romanowsky stained canine blood film containing Anaplasma platys (PCR confirmed), which are intraplatelet organisms within vacuoles. (ncvetp.org)
Ticks1
  • Among these ticks , A. marginale was detected in female ticks of R. microplus, and R. sanguineus. (bvsalud.org)
Molecular1
  • Molecular identification of A. marginale was confirmed in 120 out of 184 blood samples and 6 out of 74 tick samples. (bvsalud.org)
Strains1
  • The sequences of this entire locus were analyzed using six genetically and phenotypically distinct strains of A. marginale. (oregonstate.edu)
Bovinos1
  • O estudo teve como objetivo avaliar e comparar os aspectos clínicos, laboratoriais e patológicos de búfalos e bovinos infectados experimentalmente com estirpe AmRio 2 de Anaplasma marginale . (scielo.br)
Characterization1
  • Characterization of the Anaplasma marginale msp2 locus and its synteny with the omp1/p30 loci of Ehrlichia chaffeensis and E. canis. (oregonstate.edu)
Sequence3
  • by ELISA test, the extracted DNA from blood cells were analyzed by PCR and PCR-RFLP technique using primers derived from 16S rRNA gene and restriction endonuclease Bst 1107I enzyme which can recognize the sequence (GTATAC) in corresponding PCR product of Anaplasma marginale and cut it in the position 68 and 509, whereas the used restriction enzyme cannot cut the corresponding PCR product of other Aanaplasma spp. (academicjournals.org)
  • The obtained sequence showed similarity with A. marginale reported from Kenya and USA. (bvsalud.org)
  • Complete Genome Sequence of Anaplasma marginale subsp. (usda.gov)
Genes2
  • In this study we determined that iron is required for A. marginale replication in tick cells and identify three genes that are likely involved in iron transport. (usda.gov)
  • We then determined that all three A. marginale genes are modestly differentially expressed in response to altered host cell iron levels, despite the lack of a fur regulator or operon structure. (usda.gov)
Gene3
  • Semi-Nested-PCR (snPCR) for the msp5 and snPCR for the msp1α target gene for identification of A. marginale in blood samples from animals was done. (scielo.br)
  • Foram realizados exames clínicos, hematócrito, hemograma, esfregaço sanguíneo com avaliação de riquetsemia, necropsia e histopatologia, além de, Semi-Nested-PCR (snPCR) para o gene alvo msp5 e snPCR para o gene alvo msp1α para identificação de A. marginale nas amostras de sangue dos ruminantes. (scielo.br)
  • Interestingly, the 5' structure of this A. marginale msp2 locus is conserved in the omp1 gene locus of Ehrlichia chaffeensis and p30 gene locus of E. canis despite marked divergence between genera in the structure of the 3' region of the loci. (oregonstate.edu)
Tick1
  • Overall, occurrence of A. marginale in blood and tick samples was found to be 65.21% and 8.1% respectively. (bvsalud.org)
Strain4
  • The study aimed to evaluate and compare the clinical, laboratory and pathological aspects of buffalo and bovine experimentally infected with AmRio 2 strain of Anaplasma marginale . (scielo.br)
  • Strain AmRio 2 of A. marginale was inoculated in all experimental animals. (scielo.br)
  • However, sequencing revealed only five animals, including the bovine which died, with a similarity of the amino acid sequences with AmRio 2 strain of A. marginale . (scielo.br)
  • The complete 5.6-kb msp2 locus was identified by sequencing a 90-kb region of the St. Maries strain of A. marginale. (oregonstate.edu)
Samples1
  • The Anaplasma Pcr Mayo reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (synoviocytes.com)
Positive1
Cells2
  • The blood smear evidenced Anaplasma marginale corpuscles in parasited red blood cells. (vin.com)
  • Romanowsky stained bovine erythrocytes containing Anaplasma marginale , which are dense, homogeneously staining blue-purple inclusions 0.3-1.0 µm in diameter that are typically located toward the margins of infected cells. (ncvetp.org)
Anaplasmosis9
  • Anaplasma marginale is a vector-borne pathogen that causes a disease known as anaplasmosis. (nih.gov)
  • Anaplasmosis is a tick-borne disease caused by an obligate gram-negative bacteria, Anaplasma (A.) marginale. (lutzonilab.net)
  • Anaplasma ovis , the agent of ovine anaplasmosis, may cause mild to severe disease in sheep, deer and goats, but is not infectious for cattle. (alberta.ca)
  • Beef Cattle (over 700 lb): Control of active infection of anaplasmosis caused by Anaplasma marginale susceptible to chlortetracycline. (nih.gov)
  • Beef and Non-Lactating Dairy Cattle: As an aid in control of active infection of anaplasmosis caused by Anaplasma marginale susceptible to chlortetracycline when delivered in a free-choice feed. (nih.gov)
  • Anaplasmosis due to Anaplasma marginale, is a disease caused by a micro-organism that is a parasite of red blood cells. (canada.ca)
  • Anaplasma marginale is a virulent intra-erythrocytic pathogen that causes bovine anaplasmosis, its closely related species, Anaplasma centrale causes mild sickness. (ufs.ac.za)
  • Anaplasma marginale is the most pathogenic species and is the causative agent of severe bovine anaplasmosis. (nih.gov)
  • Anaplasma marginale es la especie más patógena y es el agente causal de la anaplasmosis bovina grave. (bvsalud.org)
Pathogens6
  • In cattle infected with Anaplasma marginale, the number of pathogens in the blood varies between a peak of 10 6 to 10 7 per mL to a low of 10 2 per mL. (medscape.com)
  • Development of a Multiplex PCR and Magnetic DNA Capture Assay for Detecting Six Species Pathogens of the Genera Anaplasma and Ehrlichia in Canine, Bovine, Caprine and Ovine. (sgu.edu)
  • Anaplasma and Ehrlichia species are tick-borne pathogens of both veterinary and public health importance. (biomedcentral.com)
  • The current status of these pathogens, including emerging species such as Ehrlichia minasensis and Anaplasma platys , infecting cattle in Kenya, remain unclear, mainly because of limitation in the diagnostic techniques. (biomedcentral.com)
  • Anaplasma and Ehrlichia species of the family Anaplasmataceae are tick-borne pathogens of livestock with some species known to infect humans and therefore are of both veterinary and public health importance [ 1 ]. (biomedcentral.com)
  • The Rocky Mountain wood tick, Dermacentor andersoni, is a vector of a number of pathogens, including Anaplasma marginale, which is the most widespread tick-borne pathogen of livestock. (pacb.com)
Bovis8
  • The serum antibodies against A. marginale and B. bovis were detected using spe- cific cELISA tests and those against B. bigemina and T. annulata by indirect immunofluorescence tests on 1764 animals (792 from Gharb area and 972 from Doukkala area) of different sexes, breed and ages. (revmedvet.com)
  • T. annulata is widely distributed in Doukkala (36.5%), while the area of Gharb is dominated by A. marginale (40.5%) as well by B. bovis (23.0%) and B. bigemina (25.0%) at a lesser extend. (revmedvet.com)
  • The Anaplasma species clustered in four distinct phylogenetic clades including A. marginale , A. platys , A. bovis and some unidentified Anaplasma spp. (biomedcentral.com)
  • Blood samples from 300 calves were collected, followed by DNA extraction and nested PCR using oligonucleotide primers to amplify fragments of the semi-nested for the msp5 gene (A. marginale), sbp-4 (B. bovis) and rap-1a (B. bigemina) Among the examined calves, the prevalence of A. marginale was 55.6% (n=167/300), B. bovis was 4.0% (n=12/300) and B. bigemina was 15.3% (n=46/300), by PCR techniques. (bvsalud.org)
  • Parasitic forms of A. marginale and B. bigemina were found in 36,3% and 2,6% of the blood smears while B. bovis was not detected. (bvsalud.org)
  • Foram coletadas 300 amostras sanguíneas de bezerros, seguidas por extração de DNA e Nested- PCR utilizando oligonucleotídeos iniciadores que amplificam fragmentos dos genes sbp-4 (B. bovis) e rap-1a (B. bigemina) e a Semi-Nested para o gene msp5 (A. marginale). (bvsalud.org)
  • A prevalência de A. marginale foi 55,66% (167/300), B. bigemina, 15,33% (46/300) e B. bovis 4,0% (12/300) dos bezerros examinados. (bvsalud.org)
  • Formas parasitárias de A. marginale and B. bigemina foram encontradas em 36,33% e 2,66% dos esfregaços sanguíneos, enquanto B. bovis não foi detectado. (bvsalud.org)
Bacteria3
  • It is an infectious disease of the red blood cells caused by the rickettsial bacteria Anaplasma marginale . (alberta.ca)
  • A. marginale is a bacteria that infects red blood cells and once an animal becomes infected they are infected for life. (beefmagazine.com)
  • Indications: Oxytetracycline is effective against gram-positive bacteria, Gram-negative and anaplasma. (petmedsmex.com)
Bigemina2
  • Furthermore, A. marginale and B. bigemina infection frequencies were significantly higher in traditional exploitations than in modern ones. (revmedvet.com)
  • There was a statistical difference between the positivity of infected animals in the age groups 1 (10-70 days) and (>70-300 days) for A. marginale and B. bigemina. (bvsalud.org)
Centrale1
  • Anaplasma centrale is a less pathogenic but closely related organism. (alberta.ca)
Ehrlichia3
  • The infection, which is caused by members of the genus Anaplasma, an obligate intracellular rickettsia-like bacterium related to the genus Ehrlichia, is characterized by repetitive cycles of rickettsemia at 6- to 8-week intervals. (medscape.com)
  • Whole blood DNA was extracted and tested for presence of Anaplasma and Ehrlichia DNA through amplification and sequencing of the 16S rDNA gene. (biomedcentral.com)
  • Anaplasma and Ehrlichia species were detected in 19.9 and 3.3% of all the samples analyzed, respectively. (biomedcentral.com)
Species2
  • It is the most pathogenic of the ANAPLASMA species. (nih.gov)
  • Anaplasma marginale transmitted by several tick vectors including some Rhipicephalus ( boophilus ) species is the most common in Kenya causing a severe hemolytic disease in the ruminants. (biomedcentral.com)
Susceptible1
  • In Anaplasma marginale transmission studies conducted on the high semi-arid range of eastern Oregon during the 1974 and 1975 vector season, A marginale-susceptible calves (principals) were maintained on 2 raised tick-proof platforms. (oregonstate.edu)
GenBank1
  • partial gene sequences amplified representation of A. marginale submitted to GenBank (Accession number MK032842 and MK032843). (lutzonilab.net)
Outer membrane1
  • Major surface protein 2 of A. marginale is an immunodominant outer membrane protein of approximately 40 kDa. (medscape.com)
Calves1
  • Two calves seronegative for A. marginale were immunized four times at weeks 0, 3, 7 and 13 with pVCL/MSP1a. (usda.gov)
Florida1
  • Reads were mapped using the genome of the Florida strain of A. marginale as a reference sequence. (nih.gov)
Humans1
  • Anaplasma marginale does not cause clinical disease in humans. (gov.ab.ca)
Significantly1
  • It was observed that the white blood cells, lymphocytes (%), monocytes (%) hematocrit, mean corpuscular hemoglobin and hemoglobin concentration of living cells on average were significantly impaired in A. marginale-positive than negative cows. (lutzonilab.net)
Presence1
  • The presence of A. marginale may be confirmed by identifying the organism in a stained blood smear from a sick animal. (alberta.ca)
Identification1
  • 2013. Identification of multilocus genetic heterogeneity in Anaplasma marginale subsp. (k-state.edu)
Blood1
Surface1
  • Immunization with the native major surface protein 1 (MSP1) (a heterodimer containing disulfide and non-covalently bonded polypeptides designated MSP1a and MSP1b) of the erythrocytic stage of A. marginale conferred protection against homologous challenge (G. H. Palmer, A. F. Barbet, W. C. Davis, and T. C. McGuire, Science, 231:1299-1302). (usda.gov)