AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
In Situ HybridizationIn Situ Hybridization, FluorescenceNucleic Acid HybridizationFluorescenceSpectrometry, FluorescenceMicroscopy, FluorescenceRNA, MessengerMolecular Sequence DataFluorescence PolarizationDNA ProbesBase SequenceFluorescent DyesRNA ProbesHybridization, GeneticImmunohistochemistryFluorescence Resonance Energy TransferPolymerase Chain ReactionCloning, MolecularAmino Acid SequenceChromosome AberrationsDigoxigeninOligonucleotide ProbesGene ExpressionChromosome MappingBlotting, NorthernGene AmplificationKaryotypingDNADNA, ComplementaryComparative Genomic HybridizationTranslocation, GeneticGene Expression Regulation, DevelopmentalChromosome BandingAneuploidySequence Analysis, DNAPhylogenyRNA, Ribosomal, 16SCarcinoma in SituReverse Transcriptase Polymerase Chain ReactionBlotting, SouthernTissue DistributionFluorescence Recovery After PhotobleachingDNA, RibosomalDNA, BacterialGene DosageMicroscopy, Fluorescence, MultiphotonDNA PrimersKineticsSequence Homology, Amino AcidOligonucleotide Array Sequence AnalysisTime FactorsPrimed In Situ LabelingSensitivity and SpecificityGreen Fluorescent ProteinsChromosomes, Human, Pair 1Transcription, GeneticChromosomes, Human, Pair 17Nucleic Acid ProbesChromosomes, Human, Pair 7DNA, ViralMicroscopy, ConfocalParaffin EmbeddingSpecies SpecificityMolecular Probe TechniquesCytogenetic AnalysisGene LibraryPeptide Nucleic AcidsCells, CulturedRNAChromosomes, Human, Pair 11Luminescent ProteinsChromosome DeletionGene Expression RegulationCarbocyaninesCell LineGene Expression ProfilingRhodaminesOrgan SpecificityInterphaseGenes, erbB-2TryptophanChromosomes, Human, Pair 8Chromosomes, Human, Pair 12Energy TransferY ChromosomeChromosomes, HumanBrainCytogeneticsRats, Sprague-DawleyChromosome PaintingMutationChromosomes, Human, Pair 14FluoresceinsChromosomes, Human, Pair 18Staining and LabelingSequence Homology, Nucleic AcidGene RearrangementCell NucleusPhenotypeChromosome Disorders
Fluorescent In Situ HybriChromosomesCytogeneticMicroscopyImmunohistochemistryKaryotypeReagentsFISHSpecimensInterphaseGenesAssayMolecularGeneticProbesAssaysTranslocationsGenomicGenomeGene expressionAbnormalitiesDiagnosisMethodsPloidyAmplificationDiagnosticsPrognosisMutationsDetectUnivariateReverseManuscriptSlidesSignalsRibosomalGermlineBioinformaticsProbeOptimalTimeDataDoseProceduresBoneFragments
Fluorescent In Situ Hybri1
  • Objectives Acute myeloid leukemia (AML) is classified in part by recurrent cytogenetic abnormalities, often detected by both fluorescent in situ hybridization (FISH) and karyotype. (medscape.com)
Chromosomes4
  • Diagnostic markers for eight Thinopyrum distichum addition chromosomes in Triticum turgidum were established using C-banding, in situ hybridization, and restriction fragment length polymorphism analysis. (nih.gov)
  • Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosomes. (wikipedia.org)
  • FISH allows the analysis of a large series of archival cases much easier to identify the pinpointed chromosome by creating a probe with an artificial chromosomal foundation that will attract similar chromosomes. (wikipedia.org)
  • Cytogenomic SNP microarray testing is used to identify genomic imbalances (deletions and duplications) and may be used to further characterize abnormalities identified by chromosome analysis, including unbalanced translocations, recombinant chromosomes, markers, and ring chromosomes. (arupconsult.com)
Cytogenetic6
  • Fluorescence In Situ Hybridization (FISH) is a powerful molecular/cytogenetic technique that utilizes a fluorescent-labeled DNA probe to ascertain the presence or absence of a particular segment of DNA. (geneticscenter.com)
  • Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity. (wikipedia.org)
  • The Cytogenetic Technologist performs a variety of tests and procedures on specimens submitted for cytogenetic analyses using cytogenetic and molecular techniques in the context of the organization's quality management system. (brainhunter.com)
  • Cytogenetic analysis would detect 60% of Wolf-Hirschhorn syndrome. (medscape.com)
  • In addition to cytogenetic analysis, testing for common single-gene mutations ( FLT3 , NPM1 , KIT , and CEBPA ) is recommended to aid in stratification of patients with newly diagnosed AML. (medscape.com)
  • It is common practice for laboratories to use interphase fluorescence in situ hybridization (FISH) panels in conjunction with karyotyping for the detection of the most prognostically significant recurrent AML-associated cytogenetic abnormalities, both in diagnostic specimens and during follow-up to monitor response to therapy. (medscape.com)
Microscopy5
  • Advances in fluorescence microscopy, fluorescent dyes, biotechnology, bioinformatics, and research on the human genome project in the late 90's accelerated the development of methods to synthesize and design NA probes for FISH. (springer.com)
  • carry out studies where biological structures or functions are visualised using methods based on fluorescence microscopy. (lu.se)
  • understand the limitations and possibilities when using different methods based on light and fluorescence microscopy. (lu.se)
  • master basic methodology using microscopy analysis of living cells. (lu.se)
  • A theoretical introduction to microscopy with an emphasis on fluorescence microscopy. (lu.se)
Immunohistochemistry2
Karyotype1
  • It is the recommended first-tier test for patients undergoing prenatal diagnosis for the indication of a fetal structural abnormality detected by ultrasound (unless the structural abnormality is strongly suggestive of a specific aneuploidy, in which case, karyotype with or without fluorescence in situ hybridization [FISH] may be offered before genomic microarray). (arupconsult.com)
Reagents2
  • DSS offers a complete solution for FISH from reagents, probes, hybridization chambers to FL microscopes with accurate filters and software. (dssimage.com)
  • After fixation, samples are permeabilized to allow the penetration of hybridization reagents. (wikipedia.org)
FISH27
  • The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
  • We further demonstrated spatially multiplexed μFISH, enabling the use of spectrally equivalent probes for detailed and real-time analysis of a cell monolayer, which paves the way towards rapid and automated multiplexed FISH on standard cytological supports. (springer.com)
  • A key milestone in ISH-based techniques was fluorescence in situ hybridization (FISH) (Bauman et al. (springer.com)
  • μFISH enables a rapid nucleic acid analysis, and we demonstrate its usefulness for spatially multiplexed FISH, Fig. 1 . (springer.com)
  • Therefore, we analyzed the potential of fluorescence in situ hybridization (FISH) for diagnosing WD, using a Tropheryma (T.) whipplei -specific probe. (frontiersin.org)
  • Currently Genetics Center offers FISH analysis for the following microdeletion syndromes: Angelman syndrome, Prader-Willi syndrome, 22q11.2 deletion syndrome and Velocardiofacial syndrome. (geneticscenter.com)
  • Hybridizer is a hands-free denaturation and hybridization instrument designed for slide-based fluorescence and chromogenic in situ hybridization (FISH and CISH, respectively) on histology and cytology samples. (dssimage.com)
  • HyperChrome is the perfect Instrument for denaturation & hybridization procedures during FISH experiments. (dssimage.com)
  • What is Fluorescence In Situ Hybridization (FISH), and how does it work? (dssimage.com)
  • Can DSS ImageTech's FISH system handle high-throughput analysis of multiple samples? (dssimage.com)
  • Does DSS ImageTech provide comprehensive image analysis and data management solutions for FISH data? (dssimage.com)
  • Fluorescence in situ hybridization (FISH) using chromosome-specific probes can be used to evaluate the ploidy of interphase nuclei. (elsevierpure.com)
  • In this study FISH was compared with flow cytometry and static image analysis in determining ploidy in paraffin-embedded tissue from 34 prostatic adenocarcinomas. (elsevierpure.com)
  • Ploidy classification as determined by FISH had an 88% concordance with ploidy classification by either flow cytometry or static image analysis. (elsevierpure.com)
  • In conclusion, FISH was found to be a sensitive method of ploidy analysis in isolated paraffin-embedded nuclei from prostate adenocarcinomas. (elsevierpure.com)
  • The authors studied DNA amplification of the HER-2/neu gene on 4-microm sections obtained from 62 formalin-fixed, paraffin-embedded PCs by fluorescence in situ hybridization (FISH). (nih.gov)
  • To investigate the possibility that a novel candidate gene for XLMR was disrupted at the X chromosome translocation breakpoint, we mapped the breakpoint using fluorescence in situ hybridisation (FISH). (bmj.com)
  • Whole chromosome painting by FISH analysis corroborated the reciprocal nature of the chromosomal exchanges between the Y chromosome and SSC13. (karger.com)
  • To develop and evaluate an automated method for quantification of HER2 fluorescence in situ hybridisation (FISH) signals. (bmj.com)
  • The FISH quantification tool (FishJ) is available for download as an ImageJ macro or alternatively it can be utilised through a web interface with an option of uploading FISH images for hybridisation signal counting. (bmj.com)
  • Fluorescence in situ hybridization (FISH) is a molecular pathological method with high sensitivity and specificity, which uses specific DNA probes to detect chromosomal aberrations, as well as gene deletion and amplification ( 21 , 22 ). (spandidos-publications.com)
  • A short introduction how cytogenetics and molecular cytogenetics were established is followed by technical aspects of fluorescence in situ hybridization (FISH). (frontiersin.org)
  • This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)
  • Bley N.: Immunofluorescence, FRAP analyses and fluorescence in situ hybridization (FISH) experiments. (forschung-sachsen-anhalt.de)
  • We compared the miRNA expression profiles of MM with 1q21 gains and MM with normal fluorescence in situ hybridisation (FISH) by gene expression array. (karger.com)
  • We carried out Al and fluorescence in situ hybridization (FISH) analyses of BRCA2 in breast tumours from germline BRCA1 mutation carriers and vice versa. (lu.se)
  • [ 6 ] The rationale for employing FISH in AML diagnosis, therefore, is to aid in the detection of cryptic rearrangements or low-level abnormalities or to identify abnormalities in cases where adequate metaphase spreads are not available for analysis. (medscape.com)
Specimens2
  • Fluorescence in situ hybridization was done with specimens obtained by bronchial brushing From 25 patients with abnormal lung shadows. (dokumen.tips)
  • These data indicate that fluorescence in situ hybridization with specimens obtained by broochial brushing can be useful for detecting numerical chromosome abnormalities and can aid in the rapid diagnosis of lung cancer. (dokumen.tips)
Interphase1
Genes2
Assay3
  • We conduct regular Hands-on Workshops for confidence building and support assay standardization in Labs and also help in the analysis of different cases. (dssimage.com)
  • Bioinformatics analysis and luciferase reporter assay were used to validate interactions among hsa_circ_0046060, miR-338-3p, and G6PC2. (hindawi.com)
  • Fluorescence in situ hybridization assay was performed to measure the expression of the lncRNA GAS5 in chondrocytes. (spandidos-publications.com)
Molecular3
  • qter confirmed by fluorescence in situ hybridisation and molecular analysis. (bmj.com)
  • Abbott Molecular ThermoBrite® System offers an easy, safe, system for in-situ hybridization procedures. (dssimage.com)
  • It provides comprehensive market analysis, highlighting the escalating use of oligonucleotide synthesis in diverse fields ranging from human health management to molecular diagnostics and therapeutics. (researchandmarkets.com)
Genetic6
  • Genetic analysis by fluorescence in situ hybridization of lung cancer cells obtained by bronchial brushing Uemura Y, Kobayashi M, Muneishi H, Urata T, Hakoda E, Tanaka Y et al, l?drd Dept. of Internal Medicine, Kochi Medical School, Kochi 783. (dokumen.tips)
  • NPM1 mutations and cytoplasmic nucleophosmin are mutually exclusive of recurrent genetic abnormalities: a comparative analysis of 2562 patients with acute myeloid leukemia. (nature.com)
  • By combining genetic and genomic analysis with whole organism and field studies we can discover the role genetic processes have in determining basic life histories and characteristics such as sex determination or disease resistance. (edu.au)
  • Phylogenetic analysis supported the morphological identification of the rodents and revealed a marked genetic difference between the two heterospecific species. (microbial-ecology.net)
  • The array CGH analyses identified a high frequency of cytogenetically cryptic genetic changes, for example, del(9p) and del(12p). (lu.se)
  • Genetic analyses have provided a wealth of information on the genectic constitution of STT, and clearly nonrandom patterns of genetic changes, ranging from point mutations and gene fusions to massive genomic imbalalnces, have been identified in each histological entity studied in sufficient detail. (lu.se)
Probes3
  • It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
  • Reverse line blot hybridization with during development of the egg, and horizontally, whereby host-specifi c probes was then used to subsequently detect the pathogen is acquired through a blood meal on a reser- and identify amplifi ed DNA. (cdc.gov)
  • Clinicopathologic features of breast cancer reclassified as HER2-amplified by fluorescence in situ hybridization with alternative chromosome 17 probes. (cdc.gov)
Assays2
  • Hüttelmaier S.: Analysis of the oncogenic potential of SR-proteins by e.g. migration, invasion and proliferation assays. (forschung-sachsen-anhalt.de)
  • Hybridization and incubation Seals ad Chambers from Grace Bio-Labs are ideally suited for in situ-hybridization assays. (gracebio.com)
Translocations2
Genomic5
  • Transcriptomic and genomic analyses of 'mutualistic' E. coli lines revealed independent mutations that disrupted the carbon catabolite repression global transcriptional regulator system. (nature.com)
  • Intriguingly, we found that the X chromosome breakpoint in the daughter could not be defined by a single breakpoint spanning genomic clone and further analysis showed a 650 kb submicroscopic duplication between DXS7067 and DXS7060 on either side of the X chromosome translocation breakpoint. (bmj.com)
  • Genomic microarray can detect chromosomal imbalances at a much higher level of resolution than standard chromosome analysis. (arupconsult.com)
  • Microarray-based comparative genomic hybridization analysis: This study is able to detect a large deletion region. (medscape.com)
  • Seventeen ETV6/RUNX1-positive pediatric acute lymphoblastic leukemias were investigated by high-resolution array-based comparative genomic hybridization ( array CGH), gene expression profiling and fluorescence in situ hybridization. (lu.se)
Genome1
  • Genotype-phenotype correlation in 21 patients with Wolf-Hirschhorn syndrome using high resolution array comparative genome hybridisation (CGH). (medscape.com)
Gene expression1
Abnormalities1
  • Chromosome analysis has limited ability to detect copy number abnormalities less than 10-15 Mb in size. (arupconsult.com)
Diagnosis2
  • Diagnosis of X-linked lymphoproliferative disease by analysis of SLAM-associated protein expression. (lu.se)
  • SH2D1A mutation analysis for diagnosis of XLP in typical and atypical patients. (lu.se)
Methods2
  • Flow cytometry and static image analysis are ploidy methods that have been used in prostate carcinoma. (elsevierpure.com)
  • Theoretical introduction to digital visualisation with an emphasis on fluorescence-based methods and digital image processing. (lu.se)
Ploidy1
  • The results were compared with HER-2/neu protein expression as determined by IHC and correlated by logistic regression analysis with Gleason tumor grade, DNA ploidy, serum prostate specific antigen (PSA), and pathological stage. (nih.gov)
Amplification2
  • Fluorescence in situ hybridisation and PCR amplification of microsatellite repeats were used to show that she had a de novo unbalanced translocation resulting in partial trisomy for 16q and partial monosomy for 15q (46,XX, -15, tder(15)t(15;16)(q26.1;q22). (bmj.com)
  • Signal amplification is achieved via series of sequential hybridization steps. (wikipedia.org)
Diagnostics1
  • ISH is performed without isolation of the targets from their source, i.e., in situ , and is widely used in research and diagnostics. (springer.com)
Prognosis1
  • All patients can evolve into trisomy 8+ MDS [12], cells, since acquiring adequate samples diagnosed with MDS have a reduced in which 8+ appears to confer a favour- for flow cytometric analysis renders the life expectancy compared with age- able prognosis [13]. (who.int)
Mutations2
  • Analysis of gene mutations and clinic features in 108 patients with myeloproliferative neoplasm]. (cdc.gov)
  • Analysis of SH2D1A mutations in patients with severe Epstein-Barr virus infections, Burkitt's lymphoma, and Hodgkin's lymphoma. (lu.se)
Detect1
  • Western blot analysis was used to detect protein expression levels of MMP‑9, MMP‑13, TIMP‑1, TIMP‑3 and type II collagen in cartilage tissue and chondrocytes. (spandidos-publications.com)
Univariate2
  • Interestingly, univariate analysis showed that after rituximab maintenance OS was not affected by IGHV status (mutated vs . unmutated OS: 85.7% alive at 7.2 years vs . 79.6% alive at 7.3 years, respectively). (haematologica.org)
  • Univariate and multivariable analyses of time to local recurrence were performed using Cox regression models. (bvsalud.org)
Reverse1
  • Reverse sequence analysis showed that the MLLT1 gene was fused to the 16p11.2 region. (cancerindex.org)
Manuscript1
  • The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)
Slides2
  • The report will provide details with respect to the conventional process of pathology laboratories, but it will specifically exclude the scope of market analysis for conventional path-lab devices (e.g., conventional microscopes, slides). (bccresearch.com)
  • Photostable nanoparticles arrayed on glass slides for calibration of fluorescence imaging systems and quantitative analysis. (gracebio.com)
Signals2
  • The hybridization signals for each probe when a nucleic abnormality is detected. (wikipedia.org)
  • Hybridisation signals were calculated both manually and automatically with the ImageJ custom macro. (bmj.com)
Ribosomal1
  • New gene-based technologies can now be employed to examine microbial diversity through the use of small sub-unit ribosomal DNA analysis (e.g. 16S rDNA) and to understand the function of complex microbial ecosystems in the rumen through metagenomic analysis. (nhbs.com)
Germline1
  • Cost-effectiveness analysis of universal germline testing for patients with pancreatic cancer. (cdc.gov)
Bioinformatics1
  • High-throughput RNA sequencing and bioinformatics analysis have led to the identification of several novel circRNAs that exhibit biological characteristics and regulatory functions [ 6 , 9 ]. (hindawi.com)
Probe1
  • After checking all the necessary conditions, hybridization steps can be started by first adding a target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s). (wikipedia.org)
Optimal1
  • It is critical for the hybridization process to have all optimal conditions to have a successful in situ result, including temperature, pH, salt concentration, and time of the hybridization reaction. (wikipedia.org)
Time1
  • The automated counting decreases the time needed for sample analysis and provides possibilities to enhance inter- and intralaboratory reproducibility of results. (bmj.com)
Data2
  • Analyses of global market trends, with data from 2012, estimates for 2013, and projections of compound annual growth rates (CAGRs) through 2018. (bccresearch.com)
  • There is a detailed discussion on market dynamics and the level of impact, key geographies and respective trends and competitive scenarios that will form the base data for qualitative analysis and market estimates. (bccresearch.com)
Dose1
  • However, additional analyses of pilots' dose from only commercial flying suggested an association (RR = 1.04 at 1 mGy, 95% CI 0.97-1.13). (cdc.gov)
Procedures1
  • The process is done in 3 main procedures: tissue preparation (pre-hybridization), hybridization, and washing (post-hybridization). (wikipedia.org)
Bone1
  • Bone marrow (BM) MRD analysis was performed after the last planned induction course and every 6 months in cases with detectable residual disease during the 36 months of maintenance therapy. (haematologica.org)
Fragments1
  • In the eventual analysis, these fragments were put into order by digesting a copy of each fragment into still smaller fragments using sequence-specific endonucleases, measuring the size of each small fragment using size-exclusion chromatography, and using that information to determine where the large fragments overlapped one another. (wikipedia.org)