Analysis fluorescent dyes. Medical search. Frequent questions

I have been wondering if anyone has done RNA primer extension analyses using fluorescent dye-labelled primers for the RT reaction, followed by analysis on e.g. an ABI sequencer. (bio.net)
To get round sensitivity problems, it might be possible to incorporate dye-labelled NTP's, rather than using dye-primers, hence increasing the fluorescence yield. (bio.net)
Since the dye binds to all amplified PCR products indiscriminately, artefacts such as those resulting from primer dimers or nonspecific binding of the primers may also contribute to the overall fluorescence. (cdc.gov)

Since trace textile fiber evidence is found at numerous crime scenes and plays an important role in linking a suspect to the respective scene, the current study examined the fluorescence of fiber dyes and fibers themselves, as well as a methodology for discriminating between fibers using room temperature fluorescence (RTF). (ojp.gov)

This qualitative in-vitro diagnostics test uses oligonucleotide probes labeled with four different fluorescent dyes. (cdc.gov)
Probe-based assays have the advantages of high specificity and the possibility to detect multiple targets in the same vessel by combining probes labeled with dyes with different fluorescent spectra. (cdc.gov)

This advanced technique uses two or more fluorescent dyes to stain chromosomes in order to detect abnormalities. (go.jp)
Following that, you would apply the set of Cell Painting dyes so that you stain and label all these different parts of the cell, and then you take your plate, and put it in a high-content imager, such as our ImageXpress® Micro High-Content Imaging System. (moleculardevices.com)
In classical banding (eg, G [Giemsa]-, Q [fluorescent]-, and C-banding), a dye is used to stain bands on the chromosomes. (msdmanuals.com)

The real-time PCR assays for parasite detection at CDC use either the DNA-binding dye SYBR Green or sequence-specific TaqMan probes as fluorescence detection mechanisms. (cdc.gov)
1 The fluorescent dye SYBR Green is added to the PCR mixture (1). (cdc.gov)
SYBR Green is a DNA binding dye that fluoresces strongly when bound to double-stranded DNA. (cdc.gov)
The most commonly used dye for fluorescent- dye based detection is SyBr Green. (cdc.gov)
The SyBr Green dye functions as an intercalating agent that emits detectable fluorescence when bound to double-stranded DNA. (cdc.gov)

The adherent cells were labeled by the fluorescent dye Hoechst 33342. (nih.gov)

Prostate specimens from subjects undergoing radical prostatectomy (RP) will be stained with a fluorescent dye (Histolog Dip) and then be scanned on a digital fluorescent confocal microscope (FCM) known as the Histolog Scanner. (who.int)

Another important tool in molecular analysis is the use of microarray analysis, which allows the expression of thousands of genes to be monitored simultaneously. (tutorialspoint.com)
The first step in DNA microarray analysis is sample preparation. (tutorialspoint.com)
The next step in DNA microarray analysis is microarray fabrication. (tutorialspoint.com)
The fifth step in DNA microarray analysis is scanning. (tutorialspoint.com)

To test this, researchers from the Biomedical Device Laboratory at Texas A&M University covalently incorporated four fluorescent dyes into the polymer backbones of polyurethane SMP foams - foams that are particularly popular due to their excellent biocompatibility. (bruker.com)

A karyotype analysis involves blocking cells in mitosis during metaphase and staining the condensed chromosomes. (msdmanuals.com)
The chromosomes are less condensed than in routine metaphase analysis, and the number of identifiable bands is expanded, allowing a more sensitive karyotype analysis. (msdmanuals.com)

[ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)

Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. (wikipedia.org)

Absorbance, excitation, and fluorescence spectra were compared between standards and extracts to determine the optimal area of the fiber to investigate dyes, fluorescent impurities, or the whole fiber. (ojp.gov)

Few synthetic cathinones are innately fluorescent, so this work posits the use of new 4-sulfo-2,3,5,6-tetrafluorophenyl ester (STP ester)-functionalized squarylium (SQ) dyes, synthesized by our collaborators in Japan, as probes for the amine-based drug targets. (wfu.edu)
As CFDA-SE, which is non-fluorescent, enters the cytoplasm of cells, intracellular esterases remove the acetate groups and convert the molecule to the fluorescent ester. (wikipedia.org)

Removal of contaminating substances such as salts, proteins, peptides, or excess derivatization reagents from your glycan samples is essential for the success of many treatments and analyses. (ludger.com)

Qualitative and quantitative analyses of the rare phenotypic variants in in vitro culture systems is necessary for the understanding of cell differentiation in cell culture of primary cells or cell lines. (nih.gov)

GeneChip analysis uses DNA labeled with fluorescent dyes to monitor gene expression. (cancerquest.org)
DNA was extracted from the blood and subjected to mutation analysis by sequencing of the PIKFYVE gene. (molvis.org)
Sequence analysis revealed that the patient has a heterozygous c.4166_4169delAAGT mutation located at exon 24 of the PIKFYVE gene that may cause p.Glu1389AspfsX16 flame-shift mutation, which has never before been reported for FCD. (molvis.org)
Molecular analysis involves the study of the molecular mechanisms underlying gene expression and regulation in transgenic organisms. (tutorialspoint.com)
One important tool in molecular analysis is the use of reporter genes, which are genes that encode for easily detectable proteins or enzymes that are used to measure gene expression levels. (tutorialspoint.com)
For example, a promoter region can be fused to a gene encoding a fluorescent protein, and the expression of the protein can be visualized using fluorescence microscopy. (tutorialspoint.com)

Determine the absorbance of your labeled sample at 260 nm ( A 260 ) and at λ max ( A dye ). (mirusbio.com)
The Label IT® dye will also contribute to the absorbance at 260 nm, which can be corrected by calculating A base . (mirusbio.com)
Note: C.F. 260 is a constant value determined by dividing the absorbance of the free Label IT® dye at 260 nm with that at λ max . (mirusbio.com)

Analysis of live and fixed cells may be based on size, granularity/complexity, fluorescent proteins, cell surface antigen expression, intracellular antigen expression, DNA content. (lu.se)

The water comes out along with the organic molecules for analysis by the instrument. (extremetech.com)
The first step in glycan analysis of glyconjugates such as glycoproteins and glycosphingolipids is the release of the sugars from the molecules to which they are attached. (ludger.com)
The DNA or RNA molecules that are labelled with fluorescent dyes are hybridized to the probes on the microarray chip. (tutorialspoint.com)

Protein analysis -Specific monoclonal antibodies ( CD9 , CD63 , and CD81 ) allow for detection of cellular and exosomal antigens by Western blot analysis . (thermofisher.com)
Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC). (cdc.gov)

The level of discrimination obtained via RTF-EEM spectroscopy was sufficient to differentiate between two types of visually indistinguishable fibers and between fibers obtained from two separate cloths of the same material and colored with the same dye reagent. (ojp.gov)

Another method of genetic analysis is Southern blotting, which is used to detect the presence and copy number of specific DNA sequences in an organism's genome. (tutorialspoint.com)
Fluorescently-labeled probes detect the amount of specific double-stranded DNA sequences while fluorescent dyes detect only the amount of double-stranded DNA. (cdc.gov)

Labeling density can be calculated easily using spectrophotometry for reactions with fluorescent Label IT® dyes. (mirusbio.com)

Due to this covalent coupling reaction, fluorescent CFSE can be retained within cells for extremely long periods. (wikipedia.org)

Shape memory polymers with visible and near-infrared imaging modalities: synthesis, characterization and in vitro analysis. (bruker.com)

Electron microscopy (EM) -One of the advantages of using Invitrogen Dynabeads magnetic separation technology to pull out pure exosomal sub-populations is that you can directly perform the EM analysis. (thermofisher.com)

Two kit formats are available: the QuantiFast Multiplex RT-PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the QuantiTect Multiplex RT-PCR +R Kit for all other cyclers. (qiagen.com)
A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines. (duke.edu)

Also, due to this stable linkage, once incorporated within cells, the dye is not transferred to adjacent cells. (wikipedia.org)
Subsequent studies revealed that the dye can be used to monitor lymphocyte proliferation, both in vitro and in vivo, due to the progressive halving of CFSE fluorescence within daughter cells following each cell division. (wikipedia.org)
In addition, unlike other methods, CFSE-labeled viable cells can be recovered for further analysis. (wikipedia.org)
Furthermore, the use of CFSE has extended beyond the immune system, with the dye being used to monitor the proliferation of many other cell types such as smooth muscle cells, fibroblasts, hematopoietic stem cells and even bacteria. (wikipedia.org)
Fig. 3: Analysis of libraries of CD38 + CCR7 - T cell clones and fluorescence barcoding to dissect the antigen-specific repertoire of effector CD4 + T cells. (nature.com)
Simple Western™ analysis of lysates (0.1 mg/mL) from 3T3 cells using LC3A/B (D3U4C) XP ® Rabbit mAb #12741. (cellsignal.com)
Simple Western™ analysis of lysates (0.1 mg/mL) from Hela cells using mTOR (7C10) Rabbit mAb #2983. (cellsignal.com)
Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells treated with Calyculin A (100 uM, 30 min) using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060. (cellsignal.com)
Flow cytometric analysis of Jurkat cells using Akt (pan) (C67E7) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype control #3900 (dashed line). (cellsignal.com)
Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using AMPKα (D5A2) Rabbit mAb #5831. (cellsignal.com)
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® mTOR siRNA II (+), using mTOR (7C10) Rabbit mAb #2983 and α-Tubulin (11H10) Rabbit mAb #2125. (cellsignal.com)
Immunofluorescent analysis of MCF-7 cells, using EXOSC2 antibody 14805-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). (thermofisher.com)
The technology has revolutionised the analysis of the movement and interactions of biomolecules in living cells. (eurekalert.org)
Slide-based cytometry combines image acquisition and data treatment, and associates the power of flow cytometry (FCM) and the resolution of the microscopic studies making it suitable for the analysis of cells with rare phenotype. (nih.gov)
The cells with a given fluorescence intensity can then be located easily on the recorded image of the culture plate for further analysis. (nih.gov)
Since the fluorescence intensity of rare events and the position of thousands of surrounding cells are recorded at the same time, the method associates the advantage of the FCM analysis and the microscopic observation. (nih.gov)
After you have these images, you would run automated image analysis, which identifies individual cells and the sub-components within these cells. (moleculardevices.com)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

At the start of the reaction, very little double stranded DNA is present, and so the fluorescent signal detected by the thermocycler is low (3). (cdc.gov)
The fluorescent dye-based method utilizes a dye that emits fluorescence when incorporating itself into double-stranded DNA. (cdc.gov)
As the PCR progresses and the quantity of double-stranded DNA increases, more dye binds to the PCR products and hence, signal intensity increases. (cdc.gov)

The team found that PhB provided the greatest fluorescence and qualitative analysis of PhB-containing foams showed that 0.5% PhB yielded the highest fluorescence intensity. (bruker.com)

Detection of PCR products in real-time can be accomplished by using fluorescent dyes or probes. (cdc.gov)
Here, we are going to look at the principles behind fluorescent dye-based detection. (cdc.gov)

Furthermore, quantitative analysis showed that PhB enabled visibility through 1 cm of blood and through soft tissue. (bruker.com)

High-resolution chromosome analysis uses special culture methods to obtain a high percentage of prophase and prometaphase spreads. (msdmanuals.com)

It is possible that incorporation of fluorescent dyes into SMPs could improve their visualization in biomedical imaging. (bruker.com)

Genetic analysis involves the study of an organism's genetic material, including its DNA, RNA, and proteins. (tutorialspoint.com)

The researchers also added the radiopaque additives barium sulphate (BaSO4) and zirconium oxide (ZrO2) to the foams, which generally enhanced the fluorescence intensity that could be achieved with each dye. (bruker.com)

CFSE was originally developed as a fluorescent dye that could be used to stably label lymphocytes and track their migration within animals for many months. (wikipedia.org)

Initial systematic analysis was conducted on dye standards and extracts taken from fibers colored with the respective dyes of interest. (ojp.gov)
High performance liquid chromatography investigations were performed to obtain detailed information on the number of dye and fluorescent components present in extracts. (ojp.gov)

Amplified DNA fragments are electrophoretically resolved on an agarose gel for analysis of results. (cdc.gov)
The cDNA fragments are then labelled with fluorescent dyes and hybridized to the probes on the microarray chip. (tutorialspoint.com)

Dr. Gau's company has introduced a molecular-analysis platform that seeks to improve upon traditional diagnostic approaches through development of a handheld point-of-care system. (genengnews.com)

Blue pseudocolor = DAPI (fluorescent DNA dye). (thermofisher.com)

Flow cytometry is ideal for multiplexing targets using multiple stainings and dyes. (lu.se)

The advantages of squarylium dyes include ease of functionalization, high molar absorptivities, narrow absorption and emission bands, and enhanced emission intensities upon analyte binding. (wfu.edu)

Dynabeads offers an array of exosome isolation products and tools for characterization and analysis of their RNA and protein content, as well as in vitro and in vivo tracing to help you further the understanding of these fascinating entities. (thermofisher.com)

To further examine the role of FSH, molecular, genetic, and immunohistological analyses were performed using ovarian tissues and primary cultured GCs from normal and PCOS model rats. (bvsalud.org)

The most common method of genetic analysis is polymerase chain reaction (PCR), which is used to amplify specific DNA sequences. (tutorialspoint.com)

One area that the company is focusing on is using the technology to arrive at high resolution analysis of an isolate and then using that data to compare the isolate with others that are associated with a public health outbreak. (genomeweb.com)

The FISH technique can not only reduce the analysis time to less than one-tenth that of the conventional one-color staining method, but improves the accuracy of the analysis. (go.jp)

For genetic analysis, peripheral blood was obtained from the patient and her sister. (molvis.org)
In transgenic organisms, genetic analysis is used to determine the presence and expression of the foreign DNA sequences that have been introduced into the genome. (tutorialspoint.com)

And then from these images, you would use automated image analysis to extract over 100-1000 parameters of the cell. (moleculardevices.com)
The FACS Aria may be used for analysis or for physically separating out cell populations based on their fluorescence labelling. (lu.se)

RNA analysis -Exosomal RNA and membrane components can be labeled using fluorescent dyes, while unincorporated dye can be easily depleted with Invitrogen spin columns. (thermofisher.com)

Complementary analysis of the hard and soft protein corona: sample preparation critically effects corona composition. (mpg.de)
For example, if a transgenic organism has been modified to express a fluorescent protein, PCR can be used to amplify the DNA sequence encoding the protein and confirm its presence in the organism's genome. (tutorialspoint.com)
The sample can be RNA, DNA, or protein, depending on the purpose of the analysis. (tutorialspoint.com)

pmol dye in sample = dye conc. (mirusbio.com)
The sample is labeled with fluorescent dyes, either directly or indirectly, and then hybridized to the probes on the microarray chip. (tutorialspoint.com)

A functional analysis of the spacer of V(D)J recombination signal sequences. (duke.edu)

The remaining DNA is stained with fluorescent dyes and images of the DNA are taken with a fluorescent microscope. (genomeweb.com)

Anatomy28

Organisms11

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes37

Disciplines and Occupations6

Technology, Industry, Agriculture8

Information Science4

Health Care4

Primers3

Examined the fluorescence1

Probes labeled2

Stain3

SYBR Green5

Hoechst1

Confocal1

Microarray analysis4

Covalently1

Chromosomes2

Hybridization1

Succinimidyl ester1

Spectra1

Ester2

Substances1

Phenotypic1

Gene6

Absorbance3

Intracellular1

Molecules3

Monoclonal antibodies2

Reagent1

Detect2

Labeling1

Coupling reaction1

Synthesis1

Microscopy1

Multiplex2

Cells18

Double stranded3

Qualitative1

Detection2

Quantitative1

Chromosome Analysis1

Visualization1

Proteins1

Fluorescence intensity1

Lymphocytes1

Extracts2

Fragments2

Molecular-analysis1

Blue1

Targets1

Advantages1

Vitro1

Tissues1

Polymerase1

Isolate1

Method1

Genetic analysis2

Cell2

Components1

Protein3

Sample2

Signal1

Images1