PolymorphismAFLPMarkersISSRPrimersPolymeraseSequencesGenetic diversityVariationNuclearGermplasmSpeciesExtractPowerStudyTypesFragment length1995Randomly amplifiedRAPDsFragmentsMolecularPrimerSequence1991DiagnosticMorphologicalBandsAnalysisSpecies based1992SubstantialGenomeDistinguishMethodsGeneticsSamplesMethodResearchServeStepMajor
Polymorphism6
- These techniques include karyotyping, random amplification of polymorphic DNA, restriction fragment length polymorphism (RFLP), DNA hybridization studies, amplified fragment length polymorphism (AFLP), and polymerase chain reaction (PCR) fingerprinting ( 12 - 17 ). (cdc.gov)
- Phylogenetic analysis of North American Armillaria species based on analysis of amplified fragment length polymorphism data suggests that A. gallica is most closely related to A. sinapina, A. cepistipes, and A. calvescens. (wikipedia.org)
- Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Single-Strand Conformation Polymorphism (SSCP) and Microsatellite DNA. (biomedcentral.com)
- D. Karam, P. Westra, S. J. Niessen, M. W. Sarah and J. E. F. Figueiredo, "Assessment of Silver-Stained AFLP Markers for Studying DNA Polymorphism in Proso Millet (Panicummiliaceum L.)," Revista Brasileira de Botanica, Vol. 29, No. 4, 2006, pp. 609-615. (scirp.org)
- Amplified fragment length polymorphism (AFLP) markers were used to detect the relationships among 12 wild accessions and 62 groundcover chrysanthemum cultivars. (ashs.org)
- sessed, out of which 249 were polymorphic revealing 96.13% polymorphism. (scirp.org)
AFLP1
- This field of genetics emerged from the availability of several studies with classical molecular markers, such as isozymes, RAPD, AFLP, and microsatellites. (intechopen.com)
Markers12
- A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
- The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. (scielo.br)
- This review gives a brief summary on the development of genetic markers including both the classical genetic markers and more advanced DNA-based molecular markers. (biomedcentral.com)
- By the 1980s, many different types of DNA molecular markers had been explored, e.g. (biomedcentral.com)
- All of these DNA-based markers contain specific advantages and have played significant roles in the evaluation of genetic diversity in farm animals. (biomedcentral.com)
- Large numbers of DNA markers and other genomic tools in proso millet can readily be developed by using genomic resources in related grasses. (scirp.org)
- Out of these, 339 amplified SSR markers in proso millet. (scirp.org)
- Of these 339 markers , 254 were highly polymorphic among the 8 proso genotypes. (scirp.org)
- The r esolving power of these 254 polymorphic SSR markers ranged from 0.25 - 14.75 with an average of 2.71. (scirp.org)
- The 254 polymorphic SSR markers amplified 984 alleles in the ranges of 50 bp to 1300 bp. (scirp.org)
- The m ajority of the SSR markers (221 of 254) amplified dinucleotide repeats. (scirp.org)
- Switchgrass genomic information seems to be the most useful for developing DNA markers in proso millet. (scirp.org)
ISSR1
- 0.76 for RAPD and 0.26 to 0.81 for ISSR marker systems. (scirp.org)
Primers2
Polymerase1
- Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
Sequences2
- We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
- These results are similar to those reported in 1992 that compared sequences of nuclear ribosomal DNA. (wikipedia.org)
Genetic diversity1
- Mitochondrial (mt) genomes, due to their properties to evolve faster than the nuclear DNA, to contain introns and mobile elements and to exhibit extended polymorphisms, are ideal tools to examine genetic diversity within fungal populations and genetically identify a species or a particular isolate. (biomedcentral.com)
Variation1
Nuclear1
- Based on a combination of mitochondrial and nuclear DNA, the following questions were addressed: 1) the phylogeography of the two O. h. robertsoni phylogroups, 2) regional and local population structure in space and time, and 3) patterns of local dispersal under different isolation-by-distance scenarios. (biomedcentral.com)
Germplasm1
- At present, DNA molecular marker techniques are widely applied in the fields of germplasm identification, phylogenetics, and genetic structural analysis. (biomedcentral.com)
Species1
- In 1973, Veikko Hintikka reported a technique to distinguish between Armillaria species by growing them together as single spore isolates on petri dishes and observing changes in the morphology of the cultures. (wikipedia.org)
Extract2
- This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
- Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
Power1
- The power of PCR is based on the fact that the amount of matrix DNA is not, in theory, a limiting factor. (intechopen.com)
Study1
Types1
- Both techniques grouped all isolates into eight previously established molecular types. (cdc.gov)
Fragment length5
- These techniques include karyotyping, random amplification of polymorphic DNA, restriction fragment length polymorphism (RFLP), DNA hybridization studies, amplified fragment length polymorphism (AFLP), and polymerase chain reaction (PCR) fingerprinting ( 12 - 17 ). (cdc.gov)
- 1990) and amplified fragment length polymorphisms (AFLPs) (Vos et al . (scialert.net)
- Phylogenetic analysis of North American Armillaria species based on analysis of amplified fragment length polymorphism data suggests that A. gallica is most closely related to A. sinapina, A. cepistipes, and A. calvescens. (wikipedia.org)
- Although restriction fragment length polymorphism analysis remains a widely used method for detecting DNA sequence polymorphism, several useful variations on the fragment length theme have recently been introduced. (justia.com)
- Guatemala, and Spain were typed by using M13 polymerase phism (RFLP), DNA hybridization studies, amplified frag- chain reaction-fingerprinting and orotidine monophosphate pyro- ment length polymorphism (AFLP), and polymerase chain phosphorylase ( URA5 ) gene restriction fragment length polymor- phsm analysis with Hha I and Sau 96I in a double digest. (cdc.gov)
19951
- Certain properties are desirable for a molecular marker such as highly polymorphic behavior, codominant inheritance, frequent occurrence in the genome, even distribution throughout the genome, selectively neutral behavior, easy access, easy and fast assay and high reproducibility (Weining and Henry, 1995). (scialert.net)
Randomly amplified1
- Phylogenetic relationships among four Pistacia species were studied by randomly amplified polymorphic DNA (RAPD) analysis. (scialert.net)
RAPDs1
- Use of random amplified polymorphic DNAs (RAPDs) in separating two sibling species of Littorina. (foliamalacologica.com)
Fragments2
- A total of 248 fragments were generated and 139 bands were polymorphic at the inter-specific level. (scialert.net)
- Total DNA samples, isolated from several dozen individuals identified on the basis of morphological and anatomical features as Lymnaea stagnalis (L.), Stagnicola corvus (Gmelin), S. palustris (O. F. Müller), S. turricula (Held), and S. occulta (Jackiewicz) were used for amplification of DNA fragments by RAPD. (foliamalacologica.com)
Molecular4
- Both techniques grouped all isolates into eight previously established molecular types. (cdc.gov)
- Discover the current applications and future prospects for molecular techniques in the detection and differentiation of mycotoxigenic fungi. (mycotoxinsite.com)
- Pena SDJ, Wrogemann K (1978) Searching for molecular defects in genetics diseases by the use of a double technique.I. Methodology and initial evaluation. (laboratoriogene.com.br)
- Pena SDJ, Quillian NM, Hamerton JL, Wrogemann K (1978) Searching for Molecular defects in genetic diseases by the use of a double labeling technique.II. (laboratoriogene.com.br)
Primer2
- A variety of parameters can affect the reaction kinetics and the success of amplification (for details see Xu and Larzul, 1991) , The specificity of the amplification depends on the primer design. (distantreader.org)
- Another recent advance in polymorphic marker analysis is single short primer PCR, or "random amplified polymorphic DNA" (RAPD) marker analysis. (justia.com)
Sequence4
- Other recently developed DNA amplification methods, such as the ligase chain reaction (LCR), self-sustained sequence replication (3SR) and Qfl replicase amplification have so far found only limited applications, but show promise for specific diagnostic applications. (distantreader.org)
- It was planned to use DNA sequence analysis in this study. (scialert.net)
- The present invention provides a method of arbitrary sequence oligonucleotide fingerprinting (ASOF), a novel technology which eliminates gel electrophoresis as a step in polymorphic marker analysis, species identification and transcriptional profiling. (justia.com)
- A certain amount of DNA sequence variation occurs naturally within a population of individuals. (justia.com)
19911
- 1991) Prime % for Listeria bothering of support interests and drawing of routine techniques. (ligaya-technologies.com)
Diagnostic1
- DNA sequencing and PCR analysis can also be performed to achieve accurate microbial identification and typing, but like current DNA typing procedures, these microbial DNA diagnostic tests require gel electrophoretic analysis, which is time consuming and labor intensive and accommodates a relatively low sample throughput. (justia.com)
Morphological1
- Kafkas and Perl-Treves (2001) addressed the taxonomic relationships and genetic variation of wild Pistacia germplasm in Turkey using morphological data and random amplified polymorphic DNA (RAPD). (scialert.net)
Bands1
- DNA bands on electrophoric gels. (ncl.edu.tw)
Analysis2
- A separate group is formed by S. palustris/turricula , although these two taxa are very similar, and RAPD analysis does not explain if they are distinct species or subspecies. (foliamalacologica.com)
- anything songs are: Statistics distribution and frequency, way, valid Listeria, operator of random groups, variety ampelography, activity and poverty, palmitoylation of fifth and central :RecDescent, and analysis of event and first awardsRelated issues with not database jobs. (ligaya-technologies.com)
Species based1
- They characterized these species based on chloroplast DNA profiles and subdivided the genus into two sections, Terebinthus ( P. atlantica , P. chinensis , P. khinjuk , P. integerrima , P. terebinthus and P. vera ) and Lentiscus ( P. lentiscus , P. mexicana , P. texana and P. weinmanniflolia ). (scialert.net)
19921
- 1992) . Some thermostable DNA polymerases possess RT activity, so making a single-tube RT-PCR more feasible, allowing reduced manipulation time and minimizing carry-over contamination. (distantreader.org)
Substantial2
- Specific areas where these DNA-based amplification methods provide substantial advantages over traditional approaches are also highlighted. (distantreader.org)
- A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Genome1
- Alu insertions have contributed to >11% of the human genome and ∼30-35 Alu subfamilies remain actively mobile, yet the characterization of polymorphic Alu insertions from short-read data remains a challenge. (shengsci.com)
Distinguish1
- In 1973, Veikko Hintikka reported a technique to distinguish between Armillaria species by growing them together as single spore isolates on petri dishes and observing changes in the morphology of the cultures. (wikipedia.org)
Methods3
- While PCR and LCR rely on temperature cycling and therefore require some investment in the appropriate equipment, 3SR and Qfl replicase amplification are isothermal methods of DNA amplification, so making potential 'field' use feasible. (distantreader.org)
- The DNA amplification methods will be described briefly. (distantreader.org)
- Random amplified polymorphic DNA (RAPD) and parsimony methods. (foliamalacologica.com)
Genetics1
- Pena, S.D.J. (1977) Double Labeling: A New Investigative Technique in Biochemical Genetics. (laboratoriogene.com.br)
Samples1
- The samples produced 46.09% polymorphic banding profiles. (scialert.net)
Method1
- RAPD-PCR method used to create the genetic fingerprints of two tilapia fish species. (edu.iq)
Research1
- Therefore, present research switched to RAPD technology to study relationships in Pistacia . (scialert.net)
Serve1
- Newly synthesized DNA molecules can serve as templates in the next cycle, thus resulting in exponential amplification. (distantreader.org)
Step1
- In the first step, the double-stranded target DNA is denatured at high temperatures, resulting in single-stranded molecules. (distantreader.org)
Major1
- [ 1 ] Pneumonia (34,000 deaths) and bloodstream infections (BSIs) (25,000) were the major causes of mortality and had substantially increased in incidence from 1990. (medscape.com)