• Predicted to be involved in isoleucyl-tRNA aminoacylation and mitochondrial translation. (mcw.edu)
  • He received his Ph.D. degree in the laboratory of Dr. En-Duo Wang in Biochemistry and Molecular Biology from Shanghai Institute of Biochemistry and Cell Biology of Chinese Academy of Science in 2008, where he studied the structural and functional interplay of transfer RNA (tRNA) and tRNA synthetase in bacteria, yeast and human. (rochester.edu)
  • Perona, J.J., Rould, M.A., Steitz, T.A. (1993) Structural basis for tRNA aminoacylation by E. coli glutaminyl-tRNA synthetase. (uvm.edu)
  • Aminoacylation is the process of adding an aminoacyl group to a compound. (wikipedia.org)
  • Pezizomycotina mitochondrial tyrosyl-tRNA synthetases (mtTyrRS) are bifunctional, with the ability to splice group I introns in addition to catalyzing aminoacylation. (utexas.edu)
  • Through the oligomerization and functional re-assignment of protein domains associated with aminoacylation and phosphate binding, the HisZ-HisG octameric ATP-PRT acquired the ability to initiate the synthesis of a key metabolic intermediate in an allosterically regulated fashion. (rcsb.org)
  • Predicted to be involved in phenylalanyl-tRNA aminoacylation. (nih.gov)
  • Out of eight mutations located in helical domains converting canonical Watson-Crick pairs into G-U pairs or C.A mismatches, six have no effect on aminoacylation (A8296G, U8316C, G8342A, U8356C, U8362G, G8363A), and two lead to drastic decreases (5000- to 7000-fold) in lysylation efficiencies (G8313A and G8328A). (nih.gov)
  • Interestingly, a C35A mutation had no effect on aminoacylation by the cysteinyl-tRNA synthetase. (caltech.edu)
  • One step that's crucial is aminoacylation, the attachment of amino acids to their proper tRNAs. (nih.gov)
  • In the November 26, 2009, issue of Nature , the team described a new method that can measure the accuracy of aminoacylation for 6 of the 20 amino acids. (nih.gov)
  • IARS is a member of aminoacyl-tRNA synthetases which catalyze the aminoacylation of tRNA by their cognate amino acid. (thermofisher.com)
  • These two results suggest that the first pairs of "complementary" amino acids that were engaged in primordial coding, such as Gly and Ala, could have avoided erroneous aminoacylation if and only if the acceptor stems of their adaptors were recognized from the same, major groove, side. (wordnik.com)
  • Remarkably, the T-boxes directly sense the aminoacylation states of bound tRNAs to gauge intracellular amino acid availability, and direct a transcriptional or translational switch to regulate amino acid biosynthesis, transport, and tRNA aminoacylation. (nih.gov)
  • The overall accuracy of the two-step aminoacylation reaction is enhanced by an editing domain that hydrolyzes mischarged tRNAs, notably ile-tRNA(leu). (rcsb.org)
  • It is increasingly clear that the aminoacylation state of tRNAs can serve as crucial indicators of overall cellular well-being in both bacteria and eukaryotes. (nih.gov)
  • Dr. Paul R. Schimmel and his team at The Scripps Research Institute conducted a series of molecular studies and deciphered a high-resolution chemical structure that reveals how the compound jams the process of aminoacylation, a crucial biological process that allows organisms to synthesize the proteins they require to live. (nih.gov)
  • Aminoacylation is the process of adding an aminoacyl group to a compound. (wikipedia.org)
  • We investigated two potentially prebiotic routes to aminoacyl-tRNA acceptor stem-overhang mimics and analyzed those oligonucleotides undergoing the most efficient aminoacylation. (emergence-of-life.de)
  • Efficient aminoacylation of the tRNA(Ala) acceptor stem: dependence on the 2:71 base pair. (irbbarcelona.org)
  • Factors determining the specificity of the tRNA aminoacylation reaction. (wikidata.org)
  • The sole mutation located in a loop of the tRNA secondary structure, A8344G, does not affect aminoacylation efficiency. (nih.gov)
  • Mutation of G322 to A or E420 to A led to an enzyme defective in aminoacylation in vitro and complementation in vivo. (ncu.edu.tw)
  • The antiterminator domain detects the molecular volume of the tRNA 3' end to sense aminoacylation. (nih.gov)
  • Our data show that the tRNACAG(Ser) emerged from insertion of an adenosine in the middle position of the 5'-CGA-3'anticodon of a tRNACGA(Ser) ancestor, producing the 5'-CAG-3' anticodon of the tRNACAG(Ser), without altering its aminoacylation properties. (nih.gov)
  • On the other hand, deletion of the C-Ala domain from CeAlaRSc yielded a truncated enzyme defective in aminoacylation. (ncu.edu.tw)
  • The accuracy of aminoacylation inside living cells, however, was unknown. (nih.gov)
  • A team led by Dr. Tao Pan at the University of Chicago and Dr. Jonathan Yewdell at NIH's National Institute of Allergy and Infectious Diseases (NIAID) set out to measure the accuracy of aminoacylation in living cells and animals. (nih.gov)
  • Overhang sequences do not significantly influence the chemoselectivity of aminoacylation by either route. (emergence-of-life.de)
  • 2. The nuclear tRNA aminoacylation-dependent pathway may be the principal route used to export tRNA from the nucleus in Saccharomyces cerevisiae. (nih.gov)
  • This has been exploited in a systematic analysis of the effects on aminoacylation of nine pathology-related mutations described so far. (nih.gov)