AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation Science
Tissue EngineeringTissue ScaffoldsCartilage, ArticularCartilagePorosityBiocompatible MaterialsCartilage DiseasesProtein EngineeringBiomedical EngineeringPolyestersChondrocytesGenetic EngineeringHydrogelsMaterials TestingNanofibersBioartificial OrgansGuided Tissue RegenerationBone RegenerationRegenerative MedicineAbsorbable ImplantsBiomimetic MaterialsDecanoatesHydrogelPolymersChondrogenesisAirway RemodelingMesenchymal Stromal CellsAirway ObstructionPolyglycolic AcidMicroscopy, Electron, ScanningCell Culture TechniquesOsteogenesisCompressive StrengthBioreactorsEar CartilageHyaline CartilageExtracellular MatrixSilkAirway ResistanceMetabolic EngineeringChitosanNasal CartilagesCollagenElastomersCells, CulturedOsteoarthritisGlycosaminoglycansBioengineeringRegenerationImplants, ExperimentalBone and BonesTensile StrengthProteoglycansCollagen Type IICalcium PhosphatesNanostructuresMicrotechnologyMechanical PhenomenaLaryngeal CartilagesAggrecansCell DifferentiationBone SubstitutesKnee JointHexuronic AcidsGlucuronic AcidBiomimeticsDurapatiteGelatinAlginatesStress, MechanicalBiomechanical PhenomenaExtracellular Matrix ProteinsSurface PropertiesMenisci, TibialStem CellsElastic ModulusFibroinsNanocompositesCartilage Oligomeric Matrix ProteinLactic AcidCell ProliferationCattleFractures, CartilageOsteoarthritis, KneeBone Morphogenetic Protein 2Hyaluronic AcidPolyurethanesTissue Culture TechniquesBronchiFibrocartilageRespiratory SystemDental PulpOsteoblastsCalcification, PhysiologicCell ShapeFemurMatrilin ProteinsNanotechnologyCell SurvivalGels
RegenerationChondrocytesConnective tissueExtracellularProgenitor CellsBiomedical EngineeringBlood vesselsUpper-airwayRegenerativeFibrousReconstruction2017CharacteristicsVivoBiocompatibleTransplantationBiochemicalTrachealBiomimeticBoneProsthesisObstructionEsophagusComplicationsCellsAllograftsCompositionIntubationBFGFStructuresConstructsFlapsCollagenMechanicalLigamentsImplantationArchitecturesOsteoarthritisDefectsMigrationStenosisHumanCompositeSurgeonsInflammatorySuccessfully2016BiologicalLung tissueUltimatelyAdjacentChronicRestorationGottardiBiomaterialsSkinTypes
Regeneration13
Chondrocytes2
  • The first tissue-engineered, ear-shaped appendages made from bovine chondrocytes and biocompatible scaffolds by the Vacanti group were prone to deformation when xenografted onto immune-compromised mice, highlighting the lack of long-term stability [ 6 ]. (biomedcentral.com)
  • A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. (lookformedical.com)
Connective tissue8
  • During inflammation, there is a disturbance of connective tissue (collagen) for release necessary healing proteins. (wikipedia.org)
  • Dynamic Compressive Loading Improves Cartilage Repair in an in vitro Model of Microfracture: Comparison of Two Mechanical Loading Regimens on Simulated Microfracture Based on Fibrin Gel Scaffolds Encapsulating Connective Tissue Progenitor Cells. (chop.edu)
  • Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
  • Between the follicles are numerous blood and lymph vessels in anoth- er connective tissue called stroma. (best10forexbrokers.com)
  • A family of structurally related collagens that form the characteristic collagen fibril bundles seen in CONNECTIVE TISSUE. (lookformedical.com)
  • A non-fibrillar collagen that forms a network of MICROFIBRILS within the EXTRACELLULAR MATRIX of CONNECTIVE TISSUE. (lookformedical.com)
  • This classification was based on the notion that "collagen" was equivalent to "connective tissue", but with the present recognition of the different types of collagen and the aggregates derived from them as distinct entities, the term "collagen diseases" now pertains exclusively to those inherited conditions in which the primary defect is at the gene level and affects collagen biosynthesis, post-translational modification, or extracellular processing directly. (lookformedical.com)
  • Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. (lookformedical.com)
Extracellular5
  • Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering. (chop.edu)
  • Extracellular vesicle DNA from human melanoma tissues contains cancer-specific mutations. (gu.se)
  • The two types of esophageal tissue derived-decellularized extracellular matrix bioinks can mimic the inherent components and composition of original tissues with layer specificity. (nature.com)
  • Electrospinning was considered a promising technology because it can recapitulate microstructures mimicking the environment of the extracellular matrix (ECM) in native tissue. (nature.com)
  • Extracellular matrix (ECM) is a dynamic tissue that contributes to organ integrity and function, and its regulation of cell phenotype is a major aspect of cell biology. (atsjournals.org)
Progenitor Cells1
  • Dr. Gottardi and his team then harvest ear cartilage progenitor cells (CPCs) with a minimally invasive biopsy, combine them with MEND, and create cartilage implants that could be a substitute for the standard laryngotracheal reconstruction. (upenn.edu)
Biomedical Engineering5
Blood vessels3
  • Attempts to do this with the use of foreign materials led to chronic infection, airway obstruction, migration of the prosthesis, erosion into major blood vessels and proliferation of granulation tissue. (biomedcentral.com)
  • Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
  • A fibrillar collagen consisting of three identical alpha1(III) chains that is widely distributed in many tissues containing COLLAGEN TYPE I. It is particularly abundant in BLOOD VESSELS and may play a role in tissues with elastic characteristics. (lookformedical.com)
Upper-airway2
  • "Prior studies by Dr. Gottardi's lab used in vitro models to demonstrate that incorporating AMPs into polymer-coated tubes can inhibit bacterial growth and modulate the upper-airway microbiome. (upenn.edu)
  • Accurately predicting LD deposition in the upper airway and entire lung is possible using mathematical expressions, but the prediction becomes more challenging for the bronchial airway and lung lobes. (bvsalud.org)
Regenerative8
  • This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
  • After more than 50 years of research, airway transplantation remains a major challenge in the fields of thoracic surgery and regenerative medicine. (biomedcentral.com)
  • Recent advances in regenerative medicine place us in a unique position to improve the quality of engineered tissue. (biomedcentral.com)
  • Combining biomimetic regenerative medicine strategies will allow us to improve tissue-engineered auricular cartilage with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
  • Due to recent advances in regenerative medicine and additive manufacturing we are entering into an age where we have the potential to replace 'like with like', by improving the quality of engineered tissue with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
  • We will use the lessons learnt from auricular cartilage tissue engineering to illustrate how combining additive manufacturing and regenerative medicine for tissue-engineering purposes can be used to create functional and durable tissue with potential to shift the paradigm in reconstructive surgery. (biomedcentral.com)
  • Journal of Tissue Engineering and Regenerative Medicine. (chop.edu)
  • The incidences of various esophageal diseases (e.g., congenital esophageal stenosis, tracheoesophageal fistula, esophageal atresia, esophageal cancer) are increasing, but esophageal tissue is difficult to be recovered because of its weak regenerative capability. (nature.com)
Fibrous2
  • Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
  • Scaffold choice, immunogenicity, degradation rate, toxicity of degradation products, host inflammatory responses, fibrous tissue formation due to scaffold degradation, and mechanical mismatches with the surrounding tissue are key issues that may affect the long-term behavior of the engineered tissue constructs and directly interfere with their primary biological functions [ 17 ]. (hindawi.com)
Reconstruction5
  • In 1997, we proposed a novel approach: the use of aortic grafts as a biological matrix for extensive airway reconstruction. (biomedcentral.com)
  • Inhibition to tracheal surgery before 1960 was explained by difficulties related to perioperative ventilation, the poor healing capacity of cartilage and, finally, the "2-cm Belsey rule" stipulating that it was not possible to remove more than 2 cm of the trachea with primary reconstruction [ 1 ]. (biomedcentral.com)
  • In 2004, the various tracheal substitutes and techniques of reconstruction were classified by Grillo into five categories: foreign materials, nonviable tissues, tracheal allotransplantation, autogenous tissues and tissue engineering [ 6 ]. (biomedcentral.com)
  • Severe cases require laryngotracheal reconstruction that involves grafting cartilage from the rib cage with an invasive surgery. (upenn.edu)
  • Journal Article Face · March 1, 2023 Background: Midvault reconstruction is an essential element of functional rhinoplasty. (duke.edu)
20171
Characteristics4
  • Acti-stain™ 555 phalloidin-labeled actin filaments retain many functional characteristics of unlabeled actin including their ability to interact with myosin. (cytoskeleton.com)
  • However, the characteristics of micro-LD deposition in the human nasal airway remains unknown, and studying it through experiments is challenging. (bvsalud.org)
  • These results indicate that micro-LD deposition characteristics in the human nasal airway are influenced by LD size and astronauts' activity level. (bvsalud.org)
  • In this study, we aimed to investigate the needle puncture process and its biomechanical characteristics by using a well establish porcine ex vivo trachea to simulate the forces involved in accessing airways during PCT at varying angular approaches. (bvsalud.org)
Vivo2
  • More recently, few clinical attempts have offered encouraging results with ex vivo or stem cell-based engineered airways and tracheal allografts implanted after heterotopic revascularization. (biomedcentral.com)
  • In a recent study in Cellular and Molecular Engineering , led by [Bioengineering] PhD student Matthew Aronson of the Gottardi Lab, the researchers went a step further and used both ex vivo and in vivo models to show how their patent-pending antimicrobial peptide-eluting endotracheal tube (AMP-ET) effectively targeted the local airway microbiota, reducing inflammation and resolving stenosis. (upenn.edu)
Biocompatible1
  • The ideal airway substitute has to be a biocompatible rigid but flexible tube that should facilitate reepithelialization, integrate with adjacent tissues and be resistant to stenosis and bacterial colonization. (biomedcentral.com)
Transplantation1
  • Transplantation of a human induced pluripotent stem cell-derived airway epithelial cell sheet into the middle ear of rats. (kyoto-u.ac.jp)
Biochemical1
  • Based on this suggested technique, we developed a bioprinted 3D esophageal structure with multi-layered features and converged with biochemical microenvironmental cues of esophageal tissue by using decellularizedbioinks from mucosal and muscular layers of native esophageal tissues. (nature.com)
Tracheal5
  • Currently, an autologous tissue composite, with or without a tracheal allograft, is the only long-term working solution for every aetiology, despite its technical complexity and setbacks. (ersjournals.com)
  • Tracheal replacement with a substitute is then necessary to achieve healthy airway repair. (ersjournals.com)
  • At the time Grillo wrote his reference textbook, tracheal replacement using the tissue-engineered technique had not yet been applied to malignant tumors, as it required the use of the patient's own cells and because several months were required to construct the graft. (biomedcentral.com)
  • Percutaneous tracheotomies (PCT) are commonly performed minimally invasive procedures involving the creation of an airway opening through an incision or puncture of the tracheal wall. (bvsalud.org)
  • Given that many procedures involve inserting a needle into the trachea without direct visualization of the tracheal wall, concerns have been raised over the needle punctures through the cartilaginous rings as compared to the space between them may result in fractured cartilage and post-tracheostomy airway complications. (bvsalud.org)
Biomimetic2
  • This study investigated the effectiveness of a biomimetic approach to scaffold design by employing geometric control. (bvsalud.org)
  • New bone filled the defected region in the biomimetic scaffolds, while the control scaffolds only presented new bone at the boundary. (bvsalud.org)
Bone7
  • β-tricalcium phosphate scaffolds are characterized by their porous three-dimensional synthetic scaffold structures that stimulate growth, migration, and differentiation in human cells leading to bone reparation. (wikipedia.org)
  • Medical imaging and 3D bioprinting can be used to create patient-specific bone scaffolds with complex shapes and controlled inner architectures. (bvsalud.org)
  • Rheumatoid arthritis (RA) is a chronic inflammatory disorder that results in the deterioration of joint cartilage and bone. (bvsalud.org)
  • This led to a more rapid restoration of cartilage thickness, increased deposition of glycosaminoglycans, and new bone tissue formation in the regenerated cartilage, in comparison to a single TAP2 treatment after a six-week period. (bvsalud.org)
  • Clinical Applications of Bone Tissue Engineering in Orthopaedic Trauma. (chop.edu)
  • Engineering in vitro vascularized bone models for drug screening and predictive toxicology. (chop.edu)
  • Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model. (kyoto-u.ac.jp)
Prosthesis1
  • Studies were classified according to the specific field of application (otologic, rhinologic, head and neck) and area of interest (surgical and preclinical education, customised surgical planning, tissue engineering and implantable prosthesis). (actaitalica.it)
Obstruction1
  • Unlike obstructive sleep apnea , CSA is caused by a lack of respiratory effort, instead of an obstruction in the airways [ 9 ]. (beyondachondroplasia.org)
Esophagus1
Complications2
  • Surgeons usually use ectopic conduit tissues including stomach and intestine, presumably inducing donor site morbidity and severe complications. (nature.com)
  • While the medical intervention is crucial for critical care and the management of acute respiratory failure, tracheostomy complications can lead to severe clinical symptoms due to the alterations of the airways biomechanical properties/structures. (bvsalud.org)
Cells9
  • We use auricular cartilage as an exemplar to illustrate how the use of tissue-specific adult stem cells, assembly through additive manufacturing and improved understanding of postnatal tissue maturation will allow us to more accurately replicate native tissue anisotropy. (biomedcentral.com)
  • In 1994, it was demonstrated that when BMMSCs were grown in culture with dexamethasone, ascorbic acid 2-phosphate, and inorganic phosphate, they differentiated into functional osteoblast-like cells. (wikipedia.org)
  • With our procedure, we have an easily accessible source for the cartilage and the cells, providing a straightforward and noninvasive treatment option with much potential. (upenn.edu)
  • Cells that are responsible for cartilage formation, which is an essential part of endochondral ossification. (beyondachondroplasia.org)
  • Fluorescent staining of actin filaments in fixed tissue sections and tissue culture cells preparations.Note: Unlike many actin antibodies, Acti-stain™ 555 phalloidin binds only to F-actin resulting in low background fluorescence. (cytoskeleton.com)
  • Phalloidin has been labeled with a proprietary red fluorescent dye which allows it to be used to stain actin filaments in tissue cultured cells and tissue sections (1, see Figure 1) and cell-free preparations. (cytoskeleton.com)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
  • The research group has already worked for several years to develop electrodes that are thin and flexible enough not to disturb the brain tissue, and with material that does not irritate the cells nearby. (regenerativemedicine.net)
  • Adaptive redox response of mesenchymal stromal cells to stimulation with lipopolysaccharide inflammagen: mechanisms of remodeling of tissue barriers in sepsis. (biomedfrontiers.org)
Allografts1
  • This led to the first human applications using cryopreserved aortic allografts that present key advantages because they are available in tissue banks and do not require immunosuppressive therapy. (biomedcentral.com)
Composition1
  • ECM is a highly dynamic complex that varies in composition according to its tissue localization and physiological circumstances. (atsjournals.org)
Intubation3
  • Gottardi's work in subglottic stensosis, a severe narrowing of the airway in response to intubation, was recently profiled in CHOP's Cornerstone Blog. (upenn.edu)
  • Subglottic stenosis is a narrowing of the airway, in response to intubation. (upenn.edu)
  • Early recognition of impending airway compromise, followed by prompt intubation, can be life saving. (medscape.com)
BFGF1
  • One classical approach to promoting vascularization is to decorate or supplement the scaffolds with proangiogenic factors such as VEGF, bFGF, and PDGF [ 8 ]. (hindawi.com)
Structures1
  • However, the engineering of more complex tissues consisting of large 3D structures remains a critical challenge because the penetration of oxygen, which is required for cell survival, is limited by diffusion to a distance of approximately 150 to 200 mm from the nearest blood vessel. (hindawi.com)
Constructs3
  • Current tissue-engineered auricular constructs implanted into immune-competent animal models have been observed to undergo inflammation, fibrosis, foreign body reaction, calcification and degradation. (biomedcentral.com)
  • Angiogenesis is an important factor for tissue-engineered skin constructs. (hindawi.com)
  • Several approaches were considered to fabricate tubular constructs for esophageal tissueengineering. (nature.com)
Flaps1
Collagen3
Mechanical2
  • Skin tissue substitutes must readily adhere, have good physical and mechanical properties, and be nonantigenic [ 2 ]. (hindawi.com)
  • Her laboratory specializes in using engineered tissues and computational models to understand how mechanical forces direct developmental patterning events during tissue morphogenesis and during disease progression. (royalsociety.org)
Ligaments1
  • We report a difference in puncture force between piercing the cartilage and the annular ligaments and observe that the angle of puncture does not significantly alter the puncture forces. (bvsalud.org)
Implantation2
  • Implantation of nonviable tissues-chemically treated, frozen or lyophilized-has been associated with poor functional results. (biomedcentral.com)
  • Along with the production of anatomical models addressed to customised surgical planning, medical teaching and surgical training, research in 3DP has explored the pioneering world of biologic tissue engineering, patient-specific implantation and ultimately of personalised pharmacoprinting. (actaitalica.it)
Architectures1
  • Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
Osteoarthritis1
Defects1
  • In 2014, George K. Sándor performed a small size clinical trial (n = 13) on patients with craniomaxillofacial defects where AMCs were transplanted with scaffolds of either bioactive glass or β-tricalcium phosphate in an attempt to reconstruct the defect. (wikipedia.org)
Migration2
  • The purpose of this study is to examine the functional heterogeneity of tonsil-derived MSCs (TMSCs) and to identify a marker to evaluate efficacy for the enhancement of BMC migration. (bvsalud.org)
  • Sustained hypoxia or lack of nutrients leads to fibroblast dysfunction, decreased keratinocyte migration and proliferation, and tissue loss [ 6 ]. (hindawi.com)
Stenosis1
  • Bioengineering researchers at Children's Hospital of Philadelphia are developing a less invasive and quicker method to create cartilage implants as an alternative to the current treatment for severe subglottic stenosis , which occurs in 10 percent of premature infants in the U.S. (upenn.edu)
Human4
  • Chondroitin sulfate is a chemical found in human and animal cartilage. (medlineplus.gov)
  • We are hopeful that our airway innovation will show similar success in human trials, so that we can improve outcomes for intubated pediatric patients. (upenn.edu)
  • Human esophageal tissue consists of the mucosa, submucosa, and muscular layers. (nature.com)
  • This methodology is expected to allow investigation of important ECM-based hypotheses in human tissues and permits future scientific exploration in an organ- and disease-specific manner. (atsjournals.org)
Composite1
Surgeons1
  • The combined efforts of cell biologists, material scientists, tissue engineers and reconstructive surgeons and associated converging technologies [ 1 ] in the 21st century have put us in an enviable position compared with our predecessors. (biomedcentral.com)
Inflammatory1
  • We engineered depots of TAP2 encapsulated in click-crosslinked hyaluronic acid (TAP2+Cx-HA) for intra-articular administration, aiming to enhance the effectiveness of TAP2 as an anti-inflammatory agent within the joint cavity. (bvsalud.org)
Successfully1
  • This study saw 10 out of the 13 patients successfully integrate the AMCs and scaffolds. (wikipedia.org)
20161
  • Dr Nelson's contributions to the fields of tissue mechanics and morphogenesis have been recognized by a number of awards, including a Burroughs Wellcome Fund Career Award at the Scientific Interface (2007), a Packard Fellowship (2008), a Sloan Fellowship (2010), the MIT TR35 (2010), the Allan P. Colburn Award (2011), a Dreyfus Teacher-Scholar Award (2012), and a Faculty Scholars Award from the Howard Hughes Medical Institute (2016). (royalsociety.org)
Biological1
  • Celeste M Nelson is a Professor in the Departments of Chemical and Biological Engineering and Molecular Biology at Princeton University. (royalsociety.org)
Lung tissue2
  • We report that the decellularization process effectively removes cellular and nuclear material while retaining native dimensionality and stiffness of lung tissue. (atsjournals.org)
  • 7) The LPS-low group, LPS-mid group, and LPS-high group showed an increase in HYP content and α-SMA expression in the lung tissue of rats. (alljournal.ac.cn)
Ultimately2
  • Most proteins are reoxidized by RNA Reconstitution II, ultimately a similar factors are as RNA immunity III engineers with promoting vital regions. (evakoch.com)
  • The data collected in this study can guide the design of relevant biomechanical feedback system during airway access procedures and ultimately help refine and optimize PCT. (bvsalud.org)
Adjacent1
  • The removal of foreign material and devitalized or contaminated tissue from or adjacent to a traumatic or infected lesion until surrounding healthy tissue is exposed. (lookformedical.com)
Chronic1
  • Engineering skin substitutes hold promise for advanced treatment of acute and chronic skin wounds [ 1 ]. (hindawi.com)
Restoration1
Gottardi4
  • Gottardi is Chief Scientist in the Pediatric Airway Frontier Program at the Children's Hospital of Philadelphia (CHOP). (upenn.edu)
  • I am honored to be recognized by Cellular and Molecular Engineering for this exciting and notable award," Dr. Gottardi said. (upenn.edu)
  • With grant support from the National Institutes of Health, Riccardo Gottardi, PhD , who leads the Bioengineering and Biomaterials (Bio 2 ) Lab at CHOP, is refining a technology called Meniscal Decellularized scaffold (MEND). (upenn.edu)
  • Riccardo Gottardi is an Assistant Professor in the Department of Pediatrics, Division of Pulmonary Medicine in the Perelman School of Medicine and in the Department of Bioengineering in the School of Engineering and Applied Science. (upenn.edu)
Biomaterials2
  • Current research in tissue engineering is geared towards elucidating the appropriate compositional elements (biomaterials, biomolecules and cell sources) as well as methods of assembly. (biomedcentral.com)
  • ACS Biomaterials Science & Engineering. (chop.edu)
Skin4
  • In this study, we constructed a scaffold-free bilayered tissue-engineered skin containing a capillary network. (hindawi.com)
  • HE staining showed that tissue-engineered skin exhibited a stratified epidermis after 7 days. (hindawi.com)
  • Moreover, skin substitutes should restore functional anatomy and physiology after treatment and healing of the wound [ 4 ]. (hindawi.com)
  • Tongues of skin and subcutaneous tissue, sometimes including muscle, cut away from the underlying parts but often still attached at one end. (lookformedical.com)
Types1
  • Different types of scaffolds containing these factors have been combined to enhance capillary formation in dermal wound healing models [ 9 , 10 ]. (hindawi.com)