• CAR is necessary for adenovirus infection. (wikipedia.org)
  • The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. (ersjournals.com)
  • These human observations are supported by experiments in guinea pigs, where, in the absence of actively replicating virus but the presence of E1A DNA, adenovirus infection resulted in excess lung inflammation following a single exposure to cigarette smoke 7 , and increased parenchymal and airway wall inflammation with greater emphysematous destruction after chronic exposure 8 . (ersjournals.com)
  • Adenovirus Type 5 DNA-Protein Complexes from Formaldehyde Cross-Linked Cells Early After Infection , 312 Virology 204 (2003) (with David Spector, Nicholas Baird & Daniel Engel). (northwestern.edu)
  • Adenovirus (AdV) encodes proteins from the early E4 region which are necessary for productive infection. (bvsalud.org)
  • Microbial infection) Antiviral activity is antagonized by oncoproteins, such as papillomavirus (HPV) protein E7 and adenovirus early E1A protein (PubMed:26405230). (neobiotechnologies.com)
  • We conclude that the association and altered regulation of IRS proteins by Ad5E1A contribute to the adenovirus-transformed phenotype and modulates viral infection in an Akt-dependent manner. (ox.ac.uk)
  • Rather, the localization of p53 to E1B containing nuclear sites observed during infection by Ad5 was prevented by mutations that impair interaction of the E1B protein with p53 and/or with the E4 Orf6 protein. (princeton.edu)
  • Understanding the interaction patterns between a particular virus and human proteins plays a crucial role in unveiling the underlying mechanism of viral infection and pathogenesis. (biomedcentral.com)
  • Virus infection involves several types of protein-protein interactions (PPIs) between the virus and its host. (biomedcentral.com)
  • The Conserved region 3 (CR3) portion of the human adenovirus (HAdV) 5 E1A protein functions as a potent transcriptional activator that induces expression of viral early genes during infection. (birmingham.ac.uk)
  • Recombinant protein expression of interest is detectable from 24 hours post infection. (9lb.cc)
  • For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR), which mainly determines the viral tropism [44]. (genemedi.com)
  • Infection process of adenovirus. (genemedi.com)
  • The vaccine developed by the Gamaleya Institute in Moscow with assistance from Russia's Defense Ministry uses a different virus -- the common cold-causing adenovirus -- that's been modified to carry genes for the "spike" protein that coats the coronavirus, as a way to prime the body to recognize if a real COVID-19 infection comes along. (indiatimes.com)
  • Meanwhile, in an interview with India Today, Dr Randeep Guleria said that nasal vaccines are preferable since they are easy to administer and give mucosal immunity that protects one from the early part of the infection. (punjabinewsonline.com)
  • The activity of the adenovirus major late promoter is substantially Increased as the infection proceeds from the early to late phase. (princeton.edu)
  • To gain insight into the regulation of this promoter, we analyzed protein-DNA interactions by in vivo DMS and DNasel footprinting during the course of adenovirus infection. (princeton.edu)
  • Little or no protein interaction at promoter sequences was detected early (5 hr) after infection but strong interactions at the major late transcription factor (MLTF/USF) binding site and at the TATA box were evident late (12 hr) after infection. (princeton.edu)
  • Nuclear extracts prepared from uninfected cells as well as cells harvested at 5 and 12 hr after infection contained similar levels of MLTF/USF footprint activity, therefore the lack of a detectable interaction early after infection is not due to reduced levels of the factor early in the viral growth cycle. (princeton.edu)
  • Meningoencephalitis and neonatal sepsis can also be caused by infection with adenovirus, enterovirus, or coxsackievirus. (medscape.com)
  • Human adenovirus type 34 (HAdV-34) infection is a recognized cause of transplant-associated hemorrhagic cystitis and, in rare cases, tubulointerstitial nephritis. (cdc.gov)
  • This represses adenovirus production by 99.99999 -100% during an AAV manufacturing run, while increasing rAAV yield, packaging efficiency and infectivity compared to both Ad5 helper plasmid or wild-type adenovirus. (oxgene.com)
  • To prevent infected cell lysis, recombinant replication deficiency virus has been developed as a gene delivery tool to replace wild-type adenovirus (recombinant AdV in "application" part). (genemedi.com)
  • In addition, since many people have immunity to adenovirus type 5 itself, 'I think that the immune system reacts not with SARS-CoV-2 but with adenovirus type 5 and the effectiveness of the vaccine may be lost. (gigazine.net)
  • mRNA-1273 is a vaccine that uses a different approach from Ad5-nCoV, in which mRNA that expresses spike proteins is directly administered to the body to target immunity. (gigazine.net)
  • One of the predominant vaccine candidates against SARS-CoV-2, AZD1222 is a chimpanzee adenovirus-vectored vaccine expressing the SARS-CoV-2 spike protein. (virology.ws)
  • While another study participant also reported neurological symptoms earlier this summer, the patient was later diagnosed with multiple sclerosis which was found to be unrelated to vaccine administration (Feuerstein 2020). (virology.ws)
  • The vaccine makes use of an adenovirus as a vector to specific the spike protein of the SARS-CoV-2 virus, the find out about authors defined. (dead-samurai.com)
  • The lab is applying its adenovirus vaccine expertise against SARS-CoV-2, utilizing a modified chimpanzee virus as a vaccine delivery vehicle to induce an immune response. (wistar.org)
  • The laboratory developed an adenovirus-based vaccine against rabies virus that can provide rapid immunity following a single administration. (wistar.org)
  • Enhanced humoral HIV-1-specific immune responses generated from recombinant rhabdoviral-based vaccine vectors co-expressing HIV-1 proteins and IL-2. (jefferson.edu)
  • While Russian officials have said large-scale production of the vaccine wasn't scheduled until September, Deputy Prime Minister Tatyana Golikova said vaccination of doctors could start as early as this month. (indiatimes.com)
  • That's a similar technology as vaccines being developed by China's CanSino Biologics and Britain's Oxford University and AstraZeneca - but unlike those companies, Russian scientists haven't published any scientific information about how the vaccine has performed in animal tests or in early-stage human studies. (indiatimes.com)
  • Bharat Biotech's nasal vaccine is a recombinant replication-deficient adenovirus vectored vaccine with a pre-fusion stabilized spike protein. (punjabinewsonline.com)
  • The government had earlier said that this nasal vaccine can catapult mass vaccination during pandemics and endemics. (punjabinewsonline.com)
  • Five serious adverse events were reported, of which two were in the vaccine group (pyrexia and transverse myelitis) and three were in the control group (autoimmune haemolytic anaemia, increased C- reactive protein and myelitis). (who.int)
  • 2 This was fol owed by other COVID-19 - ideal y through an active surveil ance system - in order vaccines that utilized various platforms, including an to determine whether the event is truly associated with a adenovirus vector-based vaccine, an inactivated vaccine vaccine or vaccination. (who.int)
  • 3 and a protein subunit vaccine. (who.int)
  • citation needed] The most commonly used group of adenoviruses is serotype 5 (Ad5), whose binding to host cells is initiated by interactions between the cellular coxsackie virus and adenovirus receptor (CAR), and the knob domain of the adenovirus coat protein trimer. (wikipedia.org)
  • In particular, adenovirus serotype 7 (Ad7) has been associated with the most severe, often fatal, disease in children ( 4 - 7 ). (cdc.gov)
  • Top panel: a simplified map of the adenovirus serotype 5 genome showing the early genes (E1-E4) and the region from which the major late transcript is produced. (genemedi.com)
  • Adenovirus Protein VII Functions Throughout Early Phase and Interacts with Cellular Proteins SET and pp32 , 79 Journal of Virology 2474 (2005) (with Yuming Xue, David Ornelles, Judy Lieberman & Daniel Engel). (northwestern.edu)
  • Rak, M. A., J. Buehler, S. Zeltzer, J. Reitsma, B. Molina, S. Terhune, and F. Goodrum, 'Human Cytomegalovirus UL135 Interacts with Host Adaptor Proteins To Regulate Epidermal Growth Factor Receptor and Reactivation from Latency. (arizona.edu)
  • We find that A3A interacts with the chaperonin-containing TCP-1 (CCT) complex, a cellular machine that assists in protein folding and function. (bvsalud.org)
  • The multidomain-containing cellular protein BS69 interacts with adenovirus E1A and several other viral and cellular factors and acts as a transcription repressor. (cancerrealitycheck.com)
  • Adenovirus 5 E1A is responsible for increased expression of insulin receptor substrate 4 in established adenovirus 5-transformed cell lines and interacts with IRS components activating the PI3 kinase/Akt signalling pathway. (ox.ac.uk)
  • To understand the molecular basis of observed regional shifts in the genome types of adenovirus type 7 (Ad7) isolated in Korea during nationwide outbreaks from 1995 to 2000, the genetic variabilities of Ad7d and Ad7l were studied by sequence analysis of hexon, fiber, E3, and E4 open reading frame (ORF) 6/7 peptides. (cdc.gov)
  • Ad7d was identified as early as 1980 in Beijing and was subsequently replaced by Ad7b, which then became the predominant genome type in China through the 1990s ( 5 ). (cdc.gov)
  • Previously, sequence variations among the different genome types of Ad7 strains have been observed at 2 variable regions of the hexon gene and in a 14.9-kDa protein encoded by an ORF in the E3 region ( 6 , 14 ). (cdc.gov)
  • It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)
  • The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can't be integrated into host genome, guaranteeing the security for subsequent operations. (9lb.cc)
  • Schematic of the adenovirus genome and adenovirus-based vectors. (genemedi.com)
  • Without integration into host genome, adenovirus genome remains in an episomal state, which guarantees the low risk of mutation (Fig. 8). (genemedi.com)
  • The recombinant genome consists essentially of Ad5 sequences, with the exception of the transforming early region 1 (E1) which is derived from Ad12. (uu.nl)
  • Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
  • Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
  • Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
  • 53:742-750, 1985), in which the 12S E1A protein is required for the complete induction of the cellular DNA replication machinery in the quiescent human epithelial cells in which adenoviruses normally replicate. (cshl.edu)
  • Viruses encode gene products that manipulate host proteins to redirect cellular processes or subvert antiviral immune responses. (bvsalud.org)
  • Some cellular antiviral proteins are known to be targeted by AdV E4 gene products, resulting in their degradation or mislocalization. (bvsalud.org)
  • An unusual cellular factor potentiates protein-DNA complex assembly between Oct-1 and Vmw65. (mcmaster.ca)
  • It is well established that the human subgroup C adenovirus type 5 (Ad5) E1B 55 kDa protein can regulate the activity and concentration of the cellular tumor suppressor, p53. (princeton.edu)
  • The transforming gene products of these viruses, such as the E1A oncoproteins of adenovirus 5, led to the identification of cellular factor p60, known as cyclin A. This research was the first demonstration of a physical link between cellular transformation and the cell cycle, thereby paving the way for the melding of these two areas of research. (newswise.com)
  • However, experimental approaches to unravel PPIs are limited by several factors, including the cost and time required, the generation, cultivation and purification of appropriate virus strains, the availability of recombinantly expressed proteins, generation of knock in or overexpression cell lines, availability of antibodies and cellular model systems. (biomedcentral.com)
  • The ubiquitously expressed E4F protein was originally identified as an E1A-regulated cellular transcription factor required for adenovirus replication. (cnrs.fr)
  • A number of PSPs have been defined over the years that include, but not limited to, promoters of prostate-specific antigen (PSA), probasin (PB), mouse mammary tumor virus (MMTV LTR), prostate-specific membrane antigen (PSMA), human glandular kallikrein 2 (hK2), and prostatic steroid-binding protein C3. (hindawi.com)
  • Due to the unobvious early symptoms and the influence of some adverse factors such as tumor heterogeneity and low immunogenicity, patients with advanced gastric cancer (AGC) cannot benefit significantly from treatments such as radical surgical resection, radiotherapy, chemotherapy, and targeted therapy. (frontiersin.org)
  • His early research includes seminal work done in 1989, demonstrating the importance of cell cycle proteins in the functioning of DNA tumor viruses. (newswise.com)
  • These data show that tumor induction by adenovirus virions is not determined only by the gene products of the transforming region. (uu.nl)
  • A simian adenoviral vector termed adenovirus C68 (AdC68) was generated as a molecular clone to express the glycoprotein of rabies virus. (wistar.org)
  • Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. (9lb.cc)
  • E1-deleted (including E1a-deleted) adenoviruses are replication defective and are commonly used as viral vectors to carry therapeutic genes for gene therapy. (hindawi.com)
  • Transduction results of various Adenovirus vectors. (9lb.cc)
  • AdCox2Lluc is a CRAd targeted against oesophageal adenocarcinoma by placing the early genes under the control of a Cox-2 promoter (adenoviruses have two early genes, E1A and E1B, that are essential for replication). (wikipedia.org)
  • The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. (9lb.cc)
  • It is ' Ad5-nCoV ' in which the gene to be incorporated into adenovirus type 5. (gigazine.net)
  • Because of this concern, many research groups have forgotten the adoption of adenovirus type 5. (gigazine.net)
  • About half of the subjects already had strong immunity to adenovirus type 5. (gigazine.net)
  • However, these side effects are within the range that can be predicted by vaccination using adenovirus type 5. (gigazine.net)
  • In fact, people with high antibody levels against adenovirus type 5 tended to have fewer side effects. (gigazine.net)
  • However, the research team also reported that the immune system's response to spike proteins was slightly diminished when there was strong immune memory for adenovirus type 5. (gigazine.net)
  • 1278\1278\BAD35103.1\Human adenovirus type 4\Human adenovirus type 4 gene for fiber protein, complete cds,isolate:1998-4439. (or.jp)
  • 1278\1278\BAD35104.1\Human adenovirus type 4\Human adenovirus type 4 gene for fiber protein, complete cds,isolate:TC-18040. (or.jp)
  • 1278\1278\BAD35105.1\Human adenovirus type 4\Human adenovirus type 4 gene for fiber protein, complete cds,isolate:TC-18465. (or.jp)
  • 884\684\AAN64737.1\Human adenovirus type 4\Human adenovirus type 4 early region 3, complete sequence. (or.jp)
  • 1205\321\AAN64738.1\Human adenovirus type 4\Human adenovirus type 4 early region 3, complete sequence. (or.jp)
  • 1791\633\AAN64739.1\Human adenovirus type 4\Human adenovirus type 4 early region 3, complete sequence. (or.jp)
  • 2297\525\AAN64740.1\Human adenovirus type 4\Human adenovirus type 4 early region 3, complete sequence. (or.jp)
  • Adenovirus infections do not normally cause hepatitis in healthy children suggesting that a completely new type of adenovirus might have been introduced into the human population. (bmj.com)
  • This review will address epigenetic regulation in members of the polyomaviruses, adenoviruses, human papillomaviruses, hepatitis B, and herpes viruses. (mdpi.com)
  • Hale, A. E., D. Collins-McMillen, E. M. Lenarcic, S. Igarashi, J. P. Kamil, F. Goodrum, and N. J. Moorman, 'FOXO transcription factors activate alternative major immediate early promoters to induce human cytomegalovirus reactivation. (arizona.edu)
  • Kim, J. Heon, D. Collins-McMillen, J. C. Buehler, F. D. Goodrum, and A. D. Yurochko, 'Human Cytomegalovirus Requires Epidermal Growth Factor Receptor Signaling To Enter and Initiate the Early Steps in the Establishment of Latency in CD34+ Human Progenitor Cells. (arizona.edu)
  • Lee, S. Hee, K. Caviness, E. R. Albright, J-H. Lee, C. B. Gelbmann, M. Rak, F. Goodrum, and R. F. Kalejta, 'Long and Short Isoforms of the Human Cytomegalovirus UL138 Protein Silence IE Transcription and Promote Latency. (arizona.edu)
  • TMEM17 (transmembrane protein 173) is a 379 amino acid protein encoded by a gene mapping to human chromosome 5. (neobiotechnologies.com)
  • Prolonged expression of Ad5E1A13S is required for the observed dramatic increase in the levels of IRS-4 mRNA and protein in Ad5E1-transformed human cell lines. (ox.ac.uk)
  • These results indicate that the E1B protein fulfills an early function that correlates efficient entry into the late phase with the localization of E1B and p53 in the nucleus of Ad5-infected normal human cells. (princeton.edu)
  • However, the task of predicting protein-protein interactions between a new virus and human cells is extremely challenging due to scarce data on virus-human interactions and fast mutation rates of most viruses. (biomedcentral.com)
  • We developed a multitask transfer learning approach that exploits the information of around 24 million protein sequences and the interaction patterns from the human interactome to counter the problem of small training datasets. (biomedcentral.com)
  • Additionally, we employ an additional objective which aims to maximize the probability of observing human protein-protein interactions. (biomedcentral.com)
  • This additional task objective acts as a regularizer and also allows to incorporate domain knowledge to inform the virus-human protein-protein interaction prediction model. (biomedcentral.com)
  • Experimental results show that our proposed model works effectively for both virus-human and bacteria-human protein-protein interaction prediction tasks. (biomedcentral.com)
  • In this work, we cast the problem of predicting virus-human protein interactions as a binary classification problem and focus specifically on emerging viruses that has limited experimentally verified interaction data. (biomedcentral.com)
  • Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [42]. (genemedi.com)
  • The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
  • The idea behind this study is to place the Ad5 E1 region in cis complementation (i.e., use E1 as a transgene) back into an E1-deleted, replication-defective adenovirus under the control of a prostate-specific promoter (PSP). (hindawi.com)
  • Beal AM, Anikeeva N, Varma R, Cameron TO, Norris PJ, Dustin ML, Sykulev Y. Protein kinase C theta regulates stability of the peripheral adhesion ring junction and contributes to the sensitivity of target cell lysis by CTL. (jefferson.edu)
  • cdc2 protein kinase is complexed with both cyclin A and B: Evidence for proteolytic inactivation of MPF. (cshlpress.com)
  • Its activation is tightly controlled by numerous other signaling proteins including protein kinase C (PKC), Sab/SH3BP, and caveolin-1. (medscape.com)
  • Adenovirus, which is a DNA virus , is a completely different kind of virus from SARS-CoV-2, which is an RNA virus , but SARS-CoV- The spike protein of 2 is produced and immunity is acquired by using this as an antigen. (gigazine.net)
  • For adenovirus replication to occur, the host cell must be induced into S phase by viral proteins interfering with cell cycle proteins. (wikipedia.org)
  • Adenovirus infections might cause the acute hepatitis in children observed. (bmj.com)
  • Adenovirus varieties have been explored extensively as a viral vector for gene therapy and also as an oncolytic virus. (wikipedia.org)
  • Of the many different viruses being explored for oncolytic potential, an adenovirus was the first to be approved by a regulatory agency, the genetically modified H101 strain. (wikipedia.org)
  • To overcome this obstacle, conditional oncolytic viruses (such as conditional replication adenovirus (CRAD)) are developed to specifically target prostate without (or with minimal) systemic toxicity due to viral self-replication. (hindawi.com)
  • The best PSP was selected to construct a prostate-specific oncolytic adenovirus (CRAD) by controlling the adenoviral E1 region. (hindawi.com)
  • Thus, E1 protein expression will be confined strictly to the prostate tissues and render this a conditional oncolytic virus (CRAD) within the prostate. (hindawi.com)
  • These molecules are fusion proteins that are made up of an antibody raised against the knob domain of the adenovirus coat protein, fused to a natural ligand for a cell-surface receptor. (wikipedia.org)
  • Production of antibodies against spike proteins was confirmed 14 days after vaccination, and a significant increase in antibody levels could be confirmed in 105 out of 108 patients by 28 days after administration. (gigazine.net)
  • Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression. (bvsalud.org)
  • We've engineered supremely tight regulation of the adenoviral late region, which is wholly responsible for producing adenovirus structural proteins. (oxgene.com)
  • Genemedi has launched a comprehensive adenovirus production service, including a variety of different adenoviral expression systems used for gene over-expression and shRNA mediated gene down-regulation, which may also allow researchers to produce adenoviruses by themselves as they need. (9lb.cc)
  • citation needed] A conditionally replicative adenovirus (CRAd) with a 24 base pair deletion in the retinoblastoma-binding domain of the E1A protein (Ad5- Δ24E3), is unable to silence retinoblastoma, and therefore unable to induce S-phase in host cells. (wikipedia.org)
  • It is postulated that this intermediate phenotype may play a major role in the remodelling of the airways in chronic obstructive pulmonary disease associated with persistence of adenovirus E1A DNA. (ersjournals.com)
  • In the study, intrapartum antibiotic prophylaxis occurred appropriately in 93.3% of cases, with 0.36 of 1000 infants developing early-onset GBS disease. (medscape.com)
  • Physical mapping of temperature-sensitive mutations of adenoviruses. (cshlpress.com)
  • E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle α-actin. (ersjournals.com)
  • E1A is critical to start viral replication by promoting transcription from rep gene promoters, P5 and P19, and facilitate viral replication by activating the early adenovirus promoters. (9lb.cc)
  • The adenoviral E1A gene is responsible for inactivation of several proteins, including retinoblastoma, allowing entry into S-phase. (wikipedia.org)
  • however, it has been widely tested in early phase trials for a variety of viruses, such as Ebola virus. (virology.ws)
  • The early phase provides all the adenoviral help needed for high fidelity, high titre AAV manufacture. (oxgene.com)
  • The late phase leads to adenovirus contamination. (oxgene.com)
  • Adenovirus transcription is a two-phase event, early and late, occurring before and after viral DNA replication, respectively (Figure. (genemedi.com)
  • Consistent with these observations, we found that E4F localizes to the mitotic spindle during the M phase of early embryos. (cnrs.fr)
  • The vaccination with recombinant protein and ade- novirus expressing A2 provided the best results, unsurpassed to superior granuloma doggedness and elimination of parasites in hepatic biopsies, as confirmed around real span PCR (Polimerase Chain Reply) scrutiny [Grimaldi et al. (daubnet.com)
  • Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. (9lb.cc)
  • Over the ensuing decades, Group B Streptococcus (GBS) replaced S aureus as the most common gram-positive organism causing early-onset sepsis. (medscape.com)
  • We developed vaccines expressing the SARS -CoV-2 spike or nucleocapsid protein. (wistar.org)
  • CAR receptor-fiber-knob adenovirus interaction and internalization process [46]. (genemedi.com)
  • To assess the immunogenicity of FomA, ICR mice were vaccinated with UV-inactivated-E. coli over-expressing FomA or a negative GFP control protein [E. coli BL21(DE3) FomA or GFP] for 9 weeks. (hifpathway.com)
  • With intrapartum antibiotic prophylaxis rates of 93.3%, 0.36 of 1000 infants developed early-onset GBS disease. (medscape.com)
  • It was initially proposed that an adenovirus mutant lacking the E1B55kDa gene, dl1520 (ONYX-015), could replicate selectively in p53 deficient cells. (wikipedia.org)
  • Consistently the levels of Bmi1 a member of the group transcription repressor which negatively regulates the expression of (Park messenger RNA levels were affected by BS69-shRNA we collected total RNA from adenovirus-infected IMR90 cells and carried out both semiquantitative and real-time reverse transcription-PCR (RT-PCR). (cancerrealitycheck.com)
  • Narita induces early senescence in IMR90 cells. (cancerrealitycheck.com)
  • The steady-state concentrations of p53 were significantly higher in cells infected by the E1B 55 kDa null mutant Hr6 or three mutants carrying small insertions in the E1B 55 kDa protein coding sequence than in Ad5-infected cells. (princeton.edu)
  • Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues. (9lb.cc)
  • Selected results of Adenovirus transduction in the adult rat cardiac cells (A), eyes (B), neuron cells (C). (9lb.cc)
  • Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. (cshlpress.com)
  • Adenovirus Protein VII Condenses DNA, Represses Transcription, and Associates with Transcriptional Activator E1A , 78 Journal of Virology 6459 (2004) (with Yvette Osheim, Yuming Xue, Margaret Emanuel, Peter Lewis, Alex Bankovich, Ann Beyer & Daniel Engel). (northwestern.edu)
  • Electrophoretic mobility shift assays were performed in order to examine nuclear factor (NF)-κB and activator protein (AP)-1 binding activities. (ersjournals.com)
  • To validate our conclusions and the efficacy of nuclear separation, we used protein localization through a combination of transformations and immunofluorescence. (bvsalud.org)
  • Short peptides added to the C-terminal end of the coat protein successfully altered viral tropism. (wikipedia.org)
  • The addition of larger peptides to the C-terminus is not viable because it reduces adenovirus integrity, possibly due to an effect on fiber trimerisation. (wikipedia.org)
  • This site falls at the 3´ end of the open reading frame (ORF) 6/7 peptides of the early region 4 (E4) (unpub. (cdc.gov)
  • Using mass spectrometric analysis insulin receptor substrate 4 (IRS-4) has been identified as a novel adenovirus 5 early region 1A (Ad5E1A)-binding protein. (ox.ac.uk)
  • Early on, while following his father's research, he became interested in the link between the effect of toxic waste on the environment and the increasing cancer rates in the Campania region in Italy. (newswise.com)
  • Using TESSA™ technology, full early region expression, which provides all the help needed for AAV production, is still enabled, but the late region gene expression is turned off, meaning there is no adenoviral structural protein production. (oxgene.com)
  • Middle panel: general structure of an early region 1 (E1)-deleted Ad vector. (genemedi.com)
  • Instead of using hand-crafted protein features, we utilize statistically rich protein representations learned by a deep language modeling approach from a massive source of protein sequences. (biomedcentral.com)
  • The fiber protein also contains an HI-loop structure, which can tolerate peptide insertions of up to 100 residues without any negative effects on adenovirus integrity. (wikipedia.org)
  • Hexon and penton structures form the capsid of AdV, and fiber protein mediates the binding of the virion to the cell surface and is a major determinant of viral tropism. (genemedi.com)
  • Interval from onset of limb weakness until reporting to CDC during 2018 ranged from 18 to 36 days, with confirmed and probable cases reported earlier than non-AFM cases. (cdc.gov)
  • Prompt recognition of signs and symptoms, early specimen collection, and complete and rapid reporting will expedite public health investigations and research studies to elucidate the recent epidemiology of AFM and subsequently inform treatment and prevention recommendations. (cdc.gov)
  • Trends in the epidemiology of early-onset sepsis show a decreasing incidence of GBS disease. (medscape.com)
  • From NCBI Gene: The protein encoded by this gene is a member of the STAT protein family. (nih.gov)
  • Early-onset sepsis is 10 to 20 times more likely to occur in premature, very low birthweight infants. (medscape.com)
  • Most cases occur in children during late summer and early fall. (cdc.gov)
  • Our data indicated that this induction of p21Cip1 protein expression by BS69-shRNA was mainly due to enhanced transcription of gene. (cancerrealitycheck.com)
  • After IPTG induction, the over-expressed FomA-6×HN fusion protein at approximately 40 kDa molecular weight was detected by SDS-PAGE with coomassie blue staining ( Fig. 2A). (hifpathway.com)