Activated leukocyte cell adhesion molecule. Medical search. Frequent questions

Glycosylation-dependent binding of galectin-8 to activated leukocyte cell adhesion molecule (ALCAM/CD166) promotes its surface segregation on breast cancer cells. (bvsalud.org)
We previously demonstrated that the activated leukocyte cell adhesion molecule ( ALCAM /CD166) can interact with galectin -8 (Gal-8) in endothelial cells . (bvsalud.org)
ALCAM is a member of the immunoglobulin superfamily that promotes homophilic and heterophilic cell - cell interactions . (bvsalud.org)
We performed binding assays by surface plasmon resonance to study the interaction between Gal-8 and the recombinant glycosylated ALCAM ectodomain or endogenous ALCAM from MDA-MB-231 breast cancer cells . (bvsalud.org)
We also analyzed the binding of ALCAM -silenced or control breast cancer cells to immobilized Gal-8 by SPR. (bvsalud.org)
We showed that recombinant glycosylated ALCAM and endogenous ALCAM from breast carcinoma cells physically interacted with Gal-8 in a glycosylation -dependent fashion displaying a differential behavior compared to non-glycosylated ALCAM . (bvsalud.org)
Moreover, ALCAM -silenced breast cancer cells exhibited reduced binding to Gal-8 relative to control cells . (bvsalud.org)
Importantly, exogenously added Gal-8 provoked ALCAM segregation, probably trapping this adhesion molecule at the surface of breast cancer cells . (bvsalud.org)
Our data indicate that Gal-8 interacts with ALCAM at the surface of breast cancer cells through glycosylation -dependent mechanisms. (bvsalud.org)
A novel heterophilic interaction between ALCAM and Gal-8 is demonstrated here, suggesting its physiologic relevance in the biology of breast cancer cells . (bvsalud.org)
Upregulation of endothelial ALCAM (activated leukocyte cell adhesion molecule) is a key element for leukocyte recruitment in neurological disease. (ox.ac.uk)
Subsequently, in a proof-of-concept study, mouse models of brain metastasis were induced by intracardial injection of brain-tropic human breast carcinoma, lung adenocarcinoma or melanoma cells to upregulate endothelial ALCAM. (ox.ac.uk)
Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) is a cell-cell adhesion protein conferring heterotypic and homotypic interactions between cells of the same type and different types. (cardiff.ac.uk)
Specifically, we studied whether ALCAM acts as both a 'seed' receptor in these tumour cells and a 'soil' receptor in peritoneal mesothelial cells during cancer metastasis. (cardiff.ac.uk)
In addition, cancer cells of gastric and pancreatic origins were used to create cell models with decreased or increased levels of ALCAM expression by genetic knocking down or overexpression, respectively. (cardiff.ac.uk)
Human peritoneal mesothelial cells were also genetically transfected to generate cell models with different profiles of ALCAM expression. (cardiff.ac.uk)
These cell models were used in the tumour-mesothelial interaction assay to assess if and how the interaction was influenced by ALCAM. (cardiff.ac.uk)
ALCAM knockdown of the mesothelial cell line MET5A rendered the cells with reduced interaction with both gastric cancer cells and pancreatic cancer cells. (cardiff.ac.uk)
Likewise, levels of ALCAM in both human gastric and pancreatic cancer cells were also a determining factor for their adhesiveness to mesothelial cells, a process that was likely to be triggered the phosphorylation of the SRC kinase. (cardiff.ac.uk)
A soluble ALCAM (sALCAM) was found to be able to inhibit the adhesiveness between cancer cells and mesothelial cells, mechanistically behaving like a SRC kinase inhibitor. (cardiff.ac.uk)
The study showed that blocking ALCAM in mice lessens the flow of B cells to the brain and slows the progression of MS. (medicalnewstoday.com)
We also know that the ligand called ALCAM, is expressed on antigen-presenting cells, as well as the inflamed tissues in the skin and GI tract. (onclive.com)
Itolizumab, a first-in-class anti-CD6 monoclonal antibody targets the CD6-activated leukocyte cell adhesion molecule (ALCAM) pathway, which modulates the activity of T cells that drive immunoinflammatory diseases, and has demonstrated early activity as a first-line therapy for patients with high-risk aGVHD in the phase 1/2 EQUATE trial (NCT03763318). (onclive.com)
CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. (exbio.cz)
CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. (exbio.cz)
Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG: Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells. (exbio.cz)
We also show that ALCAM, activated leukocyte cell adhesion molecule, is expressed transiently in gonocytes. (elsevierpure.com)
Sertoli cells and myoid cells also express ALCAM at the same stage, suggesting that ALCAM may contribute to gonocyte-Sertoli cell adhesion and migration of gonoyctes toward the basement membrane. (elsevierpure.com)
Activated leukocyte cell adhesion molecule (ALCAM or CD166) modulates bone phenotype and hematopoiesis. (jefferson.edu)
Activated leukocyte cell adhesion molecule (ALCAM) mediates cell aggregation, which is required for cell-to-cell HIV transmission. (lonza.com)

citation needed] CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. (wikipedia.org)
CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. (wikipedia.org)
citation needed] Recently, CD166 has also been used as a potential cancer stem cell marker. (wikipedia.org)
CD166 was identified as an 'inert' cancer stem cell marker for non-small cell lung cancer (NSCLC). (stemcellsciencenews.com)
CD166 is a leukocyte cell adhesion molecule known to play a role in diseases such as rheumatoid arthritis, certain cancers such as colorectal carcinoma and breast cancer, and select autoimmune diseases. (duke.edu)
Frontline: Optimal T cell activation requires the engagement of CD6 and CD166. (ox.ac.uk)
The T cell surface glycoprotein, CD6 binds CD166 in the first example of an interaction between a scavenger receptor cysteine-rich domain and an immunoglobulin-like domain. (ox.ac.uk)
At concentrations, that will block the CD6/CD166 interaction, soluble monomeric CD6 and CD166 inhibit antigen-specific human T cell responses. (ox.ac.uk)

In the first phase of the extravasation process, selectins such as L-selectin on leukocytes and E- and P-selectin on endothelial cells lead to a loose connection that permits tethering and rolling of leukocytes on the endothelium under hydrodynamic shear [ 10 ]. (biomedcentral.com)
Expression system for standard: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. (innov-research.com)
TNF-a then induces the expression of leukocyte adhesion molecules upon proximal septal capillary endothelium resulting in the recruitment of inflammatory leukocytes to the site. (cdc.gov)
Atherosclerosis starts with dysfunctional changes in the endothelium induced by disturbed shear stress which can lead to endothelial and platelet activation, adhesion of monocytes on the activated endothelium, and differentiation into proinflammatory macrophages, which increase the uptake of oxidized LDL (oxLDL) and turn into foam cells, exacerbating the inflammatory signalling. (hindawi.com)
On the basis of its potential to block anti-collagen type II (CII) antibody-induced arthritis (CAIA) and to suppress the conformational activation of β 2 -integrins in leukocytes, the present study was undertaken to investigate its interference with leukocyte adherence to cytokine-activated endothelium. (biomedcentral.com)
Leukocyte rolling and firm adhesion to the synovial endothelium in an early response to the anti-CII antibody transfer were significantly decreased in ALP-pretreated mice. (biomedcentral.com)
Preventive administration of ALP as a single dose of 100 μg per mouse led to a significant suppression of leukocyte rolling on, and of firm adhesion to, the synovial venular endothelium. (biomedcentral.com)
Cytokines have important effects on the adhesive properties of the endothelium, causing circulating leukocytes to adhere to the endothelial lining of the blood vessel wall before transmigrating to the site of inflammation. (wearecellix.com)
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. (genetex.com)
CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)

Methods Automated high-content fluorescence microscopy of co-cultured cytokine-activated FLS and autologous peripheral CD4 + T cells from patients with RA was established to quantify cell-cell interactions. (bmj.com)
Signatures of cytokine-activated FLS were significantly enriched in RA synovial tissues defined as lymphoid-rich or leucocyte-rich pathotypes, with the most prominent effects for TNF-α. (bmj.com)

Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)

When either the sphere-forming system or the adherent system is used, some of the cancer cells can be propagated but other cells are lost because of cell death/apoptosis or differentiation. (nature.com)
Results Cytokine stimulation, especially with TNF-α, led to enhanced FLS-T cell interaction resulting in cell-cell contact-dependent activation, proliferation and differentiation of T cells. (bmj.com)
It promotes the normal development and differentiation of white blood cells that play a critical role in our immune system. (nutrametrix.com)

This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. (genetex.com)

Activated by cytokines and chemokines, leukocytes and endothelial cells express distinct adhesion molecules on their cell surfaces [ 9 ]. (biomedcentral.com)
The atherosclerotic process is accelerated by a myriad of factors, such as the release of inflammatory chemokines and cytokines, the generation of reactive oxygen species (ROS), growth factors, and the proliferation of vascular smooth muscle cells. (hindawi.com)
E-selectin, also known as CD62E, is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. (wearecellix.com)
Antigen binds to IgE that is bound to tissue mast cells and blood basophils, triggering release of preformed mediators (eg, histamine, proteases, chemotactic factors) and synthesis of other mediators (eg, prostaglandins, leukotrienes, platelet-activating factor, cytokines). (msdmanuals.com)

Gal-8 is a "tandem-repeat"-type galectin , known as a matricellular protein involved in cell adhesion . (bvsalud.org)
SLP-76 7 , T cell-specific adaptor protein 8 , TCR/CD3 complex 9 and Syntenin-1. (hcdm.org)
Hem CD, Ekornhol M, Granum S, Sundvold-Gjerstad V, Spurkland A. CD6 and Linker of Activated T Cells are Potential Interaction Partners for T Cell-Specific Adaptor Protein. (hcdm.org)
Researchers report that loss of Wnt-1-induced signaling protein-2 (WISP2) in MCF7 breast cancer cells can also promote the emergence of a cancer stem-like cell phenotype characterized by high expression of CD44, increased aldehyde dehydrogenase activity and mammosphere formation. (stemcellsciencenews.com)
A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. (innov-research.com)
This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. (rockland.com)
The encoded protein contains three scavenger receptor cysteine-rich(SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. (rockland.com)
Monocyte chemoattractant protein (MCP- 1) is an essential chemokine involved in monocyte rrafficking, together with its receptor CCR2, and a major chemoattractant for monocytes to inflamed endothelial cells. (wearecellix.com)
or =0.4-0.63 s(-1), typical of many leukocyte membrane protein interactions. (ox.ac.uk)
Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)

Osorio LM, De Santiago A, Aguilar-Santelises M, Mellstedt H, Jondal M: CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM. (exbio.cz)

Mol Cell Biol. (hcdm.org)

The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found 16 significant regulated pathways in imDCs and 10 significant regulated pathways in mDCs, including the phagosome, cell adhesion signaling pathway, and inflammatory mediator regulation of TRP channels pathway. (frontiersin.org)
By doing so, it appears to switch them from a proinflammatory immune effector cell to a less inflammatory, regulatory T-cell phenotype. (onclive.com)
Pathogen-associated molecular patterns released by infecting organisms not only bind immune receptors on inflammatory cells but also bind receptors on cells in the heart ( 8 ). (frontiersin.org)
Toll-like receptors are transmembrane glycoproteins, which recognize many PAMPs with extracellular domains and aggravate the exaggerated inflammatory response to bacterial infection through activating nuclear factor (NF)-κB ( 12 ). (frontiersin.org)
Antioxidant and anti-inflammatory protection of vascular endothelial cells by synthetic mimics of glutathione peroxidase. (aisa-tx.com)
CD40 has been found to be vital in mediating a wide range of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. (neuromics.com)
These mediators cause vasodilation, increased capillary permeability, mucus hypersecretion, smooth muscle spasm, and tissue infiltration with eosinophils, type 2 helper T (TH2) cells, and other inflammatory cells. (msdmanuals.com)

Ligand-receptor pair interaction analysis identified ICAM1 on FLS as an important mediator in TNF-mediated FLS-T cell interaction. (bmj.com)
AT-hook transcription factor AKNA is accounted to coordinately regulate the expression of CD40 and its ligand, which is significant for homotypic cell interactions. (neuromics.com)
25 (19) : 9128-9140 CXC chemokine ligand-13 promotes metastasis via CXCR5-dependent signaling pathway in non-small cell lung cancer. (genetex.com)
This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)

Therefore, we investigated the in vitro effect of DHEA on the expression pattern of adhesion molecules of human endothelial cells and neutrophils. (biomedcentral.com)

CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76. (hcdm.org)
The rheumatoid arthritis (RA) synovial pannus is characterized by a number of processes, including monocyte recruitment, leukocyte-endothelial adhesion, and angiogenesis. (biomedcentral.com)
The 4A11 antigen is selectively expressed in skin, lymphoid organs, the thymus and synovium, and is upregulated on RA synovium compared with normal synovium, suggesting a role in leukocyte recruitment and homing. (biomedcentral.com)
These results suggest that H-2g and the 4A11 antigen are potent mediators of leukocyte-endothelial adhesion and monocyte recruitment, and that efforts aimed at targeting this antigen or its signaling pathways may be useful in the treatment of RA. (biomedcentral.com)
[ 1 , 2 ] The clinical picture is characterized by marked leukocytosis and localized bacterial infections that are difficult to detect until they have progressed to an extensive level secondary to lack of leukocyte recruitment at the site of infection. (medscape.com)

Aruffo A, Melnick MB, Linsley PS, Seed B. The lymphocyte glycoprotein CD6 contains a repeated domain structure characteristic of a new family of cell surface and secreted proteins. (hcdm.org)
MS-based validation using primary specimens detected 30 proteins with significantly higher abundance in patient MM cells than controls. (bmj.com)
Nine of these proteins were identified as potential immunotherapeutic targets, including five that were validated by FCM, confirming their expression on the cell surface of primary MM patient cells. (bmj.com)
Diagnosis of MM is based on morphological features, imaging studies, analysis of myeloma cell-produced proteins, and testing of the blood, urine, and bone marrow (BM). (bmj.com)
When the concentration of free radicals exceeds the neutralizing capability of the body's antioxidants, these highly reactive species can damage vital molecules such as DNA, RNA, enzymes and structural proteins and large amounts of membrane lipids though peroxidation. (nutrametrix.com)
It has been shown to protect critical molecules in the body such as lipids, proteins, and nucleic acids from free radicals and reactive oxygen species (1). (nutrametrix.com)
E-selectin recognizes and binds with low affinity to sialylated carbohydrates present on the surface proteins of certain leukocytes, causing the cells to roll along the endothelial surface of the blood vessel, as temporary interactions with exceptionally high on- and off-rates are established. (wearecellix.com)

1 2 Cell surface antigens CD38 and CD138 can be used to distinguish normal cells from clonal plasma cells, but more extensive use of immunophenotyping has been limited by a lack of universally accepted markers of MM. 3-5 Once diagnosed, the current standard-of-care for MM includes immunomodulatory drugs, proteasome inhibitors, steroids, and antibody therapies. (bmj.com)
Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)

The migration of monocytes in vitro , as with induction of HMVEC ICAM-1 expression, appeared to occur via the mitogen-activated kinase and PI3kinase pathways, but also via the Src pathway. (biomedcentral.com)
Adhesion to rhVCAM-1 under physiological flow conditions was studied with Vena8 Fluoro+ biochips, with respect to THP1, monocytes and PBMCs. (wearecellix.com)

Putative pathophysiological mechanisms linking I/R injury and liver cancer recurrence include an increased implantation of circulating cancer cells in the ischemic liver and the upregulation of proliferation and angiogenic factors following the ischemic insult. (123dok.net)
Concomitantly, ALP suppressed the IL-1β-induced NF-κB activation and the upregulation of E-selectin expression in glEND.2 cells in vitro . (biomedcentral.com)
These findings support the notion that the newly uncovered properties of ALP to interfere with cytokine signalling and upregulation of adhesion molecules in endothelial cells are likely to contribute to the therapeutic potential of ALP in immune-complex-induced tissue injury. (biomedcentral.com)

May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. (eiaab.com)
The potential of recombinant ALP to block the interactions of leukocytes with the endothelial lining was concomitantly investigated in vitro and in vivo . (biomedcentral.com)

After two, four and 24 hours, fluorescence activated cell sorter (FACS) analysis for vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and E-selectin was performed. (biomedcentral.com)
We have also shown that H-2g significantly upregulates cell surface expression of the adhesion molecule intercellular adhesion molecule-1 (ICAM-1) on human dermal microvascular endothelial cells (HMVECs), but not vascular cellular adhesion molecule-1 or E-selectin. (biomedcentral.com)

19 (5) : 1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway. (genetex.com)

Cell adhesion molecule that binds to CD6. (eiaab.com)
This promotes the firm adhesion of the leukocytes through integrin-mediated binding, such as VLA-4 that binds to VCAM- 1, or LFA-1 to ICAM-1. (wearecellix.com)

These results suggest that the H-2g and the 4A11 antigen are mediators of leukocyte-endothelial adhesion via ICAM-1. (biomedcentral.com)
e lymphatic blood vascular endothelial cells are activated by several in�ammatory mediators (e.g., vascular endothelial growth capillariestakeupthisprotein-rich�uid,therebymaintaining factor- (VEGF-)A, tumor necrosis factor (TNF-) , inter- not only tissue �uid homeostasis but also exerting immune leukin (IL)-6, and IL-1), leading to the typical signs of surveillance. (sagepub.com)

In accord with this, H-2g induces ICAM-1-mediated leukocyte-endothelial adhesion. (biomedcentral.com)
27 (11) : 1509-1522 CX3CL1 induces cell migration and invasion through ICAM-1 expression in oral squamous cell carcinoma cells. (genetex.com)

Scientists showed that MMTV-Her2/Neu mammary tumor cells cultured as non-adherent spheres or as adherent monolayer cells select for stabilizing mutations in p53 that "immortalize" the cultures, and that after serial passages, sphere conditions maintain tumor-initiating cells whereas monolayer cells gradually lose these tumorigenic cells. (stemcellsciencenews.com)
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. (idrblab.net)

An 'Inert' Cancer Stem Cell Marker for Non-Small Cell Lung Cancer? (stemcellsciencenews.com)

MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)

When we placed dissociated glioma tissues on uncoated culture dishes, some cells formed sphere-like aggregates, whereas other cells grew out and extended cellular processes ( Fig. 1a ). (nature.com)
The extravasation of leukocytes from the vascular bed into surrounding tissues and organs is part of the host defense mechanisms against invading pathogens. (biomedcentral.com)
Under physiological conditions, the major 4.TheRoleofBloodandLymphaticVessels functions of blood vessels include the supply of gases, �uid, in�n�ammation nutrition, and signaling molecules to the tissues with the Boththebloodandthelymphaticvascularsystemcontribute capillaries as the actual sites of exchange. (sagepub.com)

Fluorescence-activated cell sorting and cDNA-microarray analyses revealed that each subclone was composed of distinct populations of cells. (nature.com)
Thus, intravital fluorescence microscopic imaging of leukocyte rolling and firm adhesion to postcapillary venules were performed in the knee joints of DBA1/J mice after intravenous injection of anti-CII mAbs. (biomedcentral.com)
Fluorescence-activated cell sorting was applied to determine endothelial E-selectin expression. (biomedcentral.com)

No response of either melanoma cell line to lipopolysaccharide was observed. (biomedcentral.com)

Activated FLS are increasingly recognised as a critical component driving synovial tissue remodelling by interacting with immune cells resulting in distinct synovial pathotypes of RA. (bmj.com)
Phenotypic profiling of cytokine-treated FLS and co-cultured T cells was done by flow cytometry and RNA-Seq, which were integrated with publicly available transcriptomic data from patients with different histological synovial pathotypes. (bmj.com)
FLS cytokine signatures correlated with the number of infiltrating CD4 + T cells in synovial tissue of patients with RA. (bmj.com)
86 : 104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling. (genetex.com)

Both, ICAM1 and its receptors were overexpressed in TNF-treated FLS and co-cultured T cells. (bmj.com)
In the activated state, integrins bind tightly to complementary receptors expressed on endothelial cells. (wearecellix.com)
These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)

Gimferrer I, Farnós M, Calvo M, Mittelbrunn M, Enrich C, Sánchez-Madrid F, Vives J, Lozano F. The accessory molecules CD5 and CD6 associate on the membrane of lymphoid T cells. (hcdm.org)
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCAM1 / CD106 antibody [N1N2], N-term (GTX110684) diluted at 1:20000. (genetex.com)

The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)

This Mouse Endothelial Leukocyte Adhesion Molecule 1 (E-Selectin) ELISA Kit from Innovative Research is intended for quantitative detection of mouse soluble E-Selectin in cell culture supernates, serum and plasma (heparin, citrate). (innov-research.com)
Iron-containing particles have been proposed as a mechanism of introducing unregulated iron into cells, and soluble iron has been shown to induce the expression ofTNF-a in Kupffer cells, which was preceded by an increase in hydroxyl radicals. (cdc.gov)
For donors 3 and 4, primary CD4+ T cells were activated for 2 d with 5 µg/ml soluble anti-CD28 (Tonbo Biosceiences) on plates that were coated overnight with 10 µg/ml anti-CD3 (Tonbo Biosciences). (lonza.com)
Vitamin E is a fat-soluble antioxidant that protects vulnerable fatty acids in cell membranes from lipid peroxidation. (nutrametrix.com)
Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)

Description: A competitive ELISA for quantitative measurement of Human Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (prionics.com)
Another reported type of leukocyte adhesion deficiency involves dysfunction in platelet aggregation in addition to a defect in leukocyte adhesion. (medscape.com)

On histopathology, this fibroinflammatory disease is characterized with specific findings such as lymphoplasmacytic infiltration of IgG4-positive plasma cells, obliterative phlebitis, and storiform fibrosis, in most cases accompanied by increased levels of serum IgG4 [ 10 ]. (hindawi.com)

A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models. (ox.ac.uk)
The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)

Selective activation of cervical microvascular endothelial cells by human papillomavirus 16-E7 oncoprotein. (aisa-tx.com)

These adhesion molecules enable the deceleration of blood cells on the endothelial layer in order to enable subsequent diapedesis. (biomedcentral.com)
An IL-1β-activated endothelial layer formed by a murine glomerular cell line (glEND.2) was used to assay the interaction with human leukocytes in vitro . (biomedcentral.com)

Adhesion molecules are important for the extravasation of neutrophils into organs where they may exhibit detrimental effects. (biomedcentral.com)
Neutrophils were freshly isolated from blood of 10 male healthy volunteers, stimulated the same way as endothelial cells and analyzed for surface expression of L-selectin, CD11b and CD18. (biomedcentral.com)

CD6 is expressed in T cells 1 , medullary thymocytes 1 , a subset of NK cells 2 , some hematopoietic progenitors 3 and a small group of B lymphocytes. (hcdm.org)
A complication of allogeneic hematopoietic stem cell transplant, aGVHD occurs in approximately 50% of patients who undergo this procedure. (onclive.com)
E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). (innov-research.com)

Human IgG antibody Laboratories manufactures the viral brain disease activated by monoclonal antibodies reagents distributed by Genprice. (prionics.com)
The Viral Brain Disease Activated By Monoclonal Antibodies reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (prionics.com)

Moreover, H-2g or the 4A11 antigen activate the endothelial janus kinase 2 (JAK2)/STAT3 pathway, as demonstrated by inhibition by chemical inhibitors of these pathways as well as antisense oligodeoxynucleotides directed against these signaling intermediates. (biomedcentral.com)

Here, we analyzed the physical interaction between both molecules in breast cancer cells and the functional relevance of this phenomenon. (bvsalud.org)
Computational prediction and knock-down experiments were performed in FLS to identify adhesion molecules for cell-cell interaction. (bmj.com)
Knock-down of ICAM1 in FLS resulted in reduced TNF-mediated FLS-T cell interaction. (bmj.com)

Because most cancers have a clonal origin, cancer stem cells (CSCs) must generate phenotypically diverse progenies including mature CSCs that can self-renew indefinitely and differentiated cancer cells that possess limited proliferative potential. (nature.com)
In laboratory settings, glioma stem cells (GSCs) can be isolated by adding EGF and FGF to suspension cultures in the absence of serum, which is the so-called sphere-forming method, or by using adherent culture systems in which specific materials are coated on culture dishes 4 , 6 . (nature.com)
In the presence of a genetic lesion, downregulation of SETD2 contributed to both initiation and progression during leukemia development by promoting the self-renewal potential of leukemia stem cells. (stemcellsciencenews.com)
Investigators found that spontaneously-formed tumorigenic hybrids between bone marrow-derived mesenchymal stem cells and three different non-small cell lung cancer cell lines contributed to highly malignant subpopulations with both epithelial-to-mesenchymal transition (EMT) and stem cell-like properties. (stemcellsciencenews.com)
Metformin was preferentially cytotoxic to CD44 high /CD24 low cells of MCF-7 cells and, CD44 high /CD24 high cells of MIA PaCa-2 cells, which are known to be cancer stem cells of MCF-7 cells and MIA PaCa-2 cells, respectively. (stemcellsciencenews.com)
This review summarizes the current understanding of the regulatory mechanisms of epithelial-mesenchymal transition (EMT) in cancer stem cells (CSC) initiation, with a special focus on the role of microRNAs in EMT control, and a discussion the implications of targeting CSCs for cancer therapeutics. (stemcellsciencenews.com)
The authors report the results of several published discontinuation studies and critically discuss the new approaches and tools to monitor residual disease during treatment and new strategies to target leukemic stem cells to reach a potential "operational" cure and persistent long-term leukemia-free survival. (stemcellsciencenews.com)

Scientists showed that a deep surgical margin was resected very close to the tumor site in many neoadjuvant chemotherapy (NAC)-treated oral squamous cell carcinoma patients, suggesting that NAC may lead to local recurrence and poor outcomes. (stemcellsciencenews.com)

This 3-year-old girl had leukocyte adhesion deficiency type I (LAD I) with complete absence of CD18 expression. (medscape.com)
Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)

Our results suggest that single cell-derived subclones from a patient can produce phenotypically heterogeneous self-renewing progenies in both in vitro and in vivo settings. (nature.com)
Regardless of the nomenclatures used, the CSCs (as the cells are commonly termed) that are isolated directly from patients' tumors are considered to serve as valuable tools that can enhance our understanding of tumorigenesis, therapeutic resistances and the functional heterogeneity of cancers in vitro and in vivo 5 . (nature.com)
Cellix's VenaFlux platform enables in vitro cell-based flow assays to study adhesion systems under physiological shear stress conditions, given the importance of shear stress in many steps of the adhesion cascade. (wearecellix.com)

Furthermore, clarified culture supernatants recovered from C- Fe-treated macrophages raise vascular endothelial cells to an activated state as indicated by the induction of endothelial- leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin. (cdc.gov)

That is part of the mechanism [of action of the agent,] and interfering with the CD6 costimulation is another way of blunting the inappropriate immune activity that we believe is part of the underlying pathology in GVHD," explained Koreth, who is the director of Translation Research and Stem Cell Transplantation and professor of medicine at Harvard Medical School. (onclive.com)
This effect goes along with reduced amounts of infiltrating immune cells in organs such as lung and liver. (biomedcentral.com)
After multiple trauma and/or sepsis the immune system becomes highly activated. (biomedcentral.com)
Mepolizumab targets multiple immune cells in aspirin-exacerbated respiratory disease. (harvard.edu)
Introduction Structural reorganisation of the synovium with expansion of fibroblast-like synoviocytes (FLS) and influx of immune cells is a hallmark of rheumatoid arthritis (RA). (bmj.com)

The presence of B lymphocytes in cerebrospinal fluid is a classic marker of MS, and it is these cells that give MS its progressive nature. (medicalnewstoday.com)

A better understanding of the MM cell surface proteome could facilitate development of new directed therapies and assist in stratification and monitoring of patient outcomes. (bmj.com)
Methods In this study, we first used a mass spectrometry (MS)-based discovery-driven cell surface capture (CSC) approach to map the cell surface N -glycoproteome of MM cell lines. (bmj.com)
Results We identified 696 MM cell surface N -glycoproteins by CSC, and developed 73 targeted MS detection assays. (bmj.com)
Selectins are a family of single-chain transmembrane glycoproteins, expressed on the surface of leukocytes, platelets and activated endothelial cells. (wearecellix.com)

In the present study, we were able to demonstrate effects of DHEA on the expression of every adhesion molecule investigated. (biomedcentral.com)
Human Papillomavirus-16-E7 Oncoprotein Enhances the Expression of Adhesion Molecules in Cervical Endothelial Cells but not in Human Umbilical Vein Endothelial Cells. (aisa-tx.com)
Tripterine, inhibits the expression of adhesion molecules in activated endothelial cells. (aisa-tx.com)
In milder forms of leukocyte adhesion deficiency I (1-30% expression of CD8), patients may survive to adulthood. (medscape.com)
This leukocyte adhesion deficiency variant is associated with defective expression of the Rap-1 activator CalDAG-GEFI. (medscape.com)

Leukocyte adhesion deficiency (LAD) is a rare primary immunodeficiency. (medscape.com)
Labial ulceration from which Escherichia coli was cultured in an 8-month-old girl with leukocyte adhesion deficiency type 1 (LAD I). Note the thin bluish scar at the superior aspect of the labia from an earlier cellulitis. (medscape.com)
This 10-month-old patient with severe leukocyte adhesion deficiency type I (LAD I) developed a cervical adenitis caused by Klebsiella pneumoniae. (medscape.com)
However, IgM and IgG serum levels are within the reference range in patients with leukocyte adhesion deficiency II. (medscape.com)
Leukocyte adhesion deficiency II may be classified as one of the congenital disorders of glycosylation (CDG), a rapidly expanding group of metabolic syndromes with a wide symptomatology and severity. (medscape.com)

Background Multiple myeloma (MM) is characterized by clonal expansion of malignant plasma cells in the bone marrow. (bmj.com)

Although this heterogeneity is generally discussed in terms of pathological structures, examining the dynamic heterogeneity at the cellular level is fundamental to understanding the origins of the cells, potential therapeutic targets and the source of tumor recurrences 3 . (nature.com)

We validated these pathways in primary human CD4+ T cells through Cas9-mediated knockout and antibody blockade. (lonza.com)

Bacteriophages, bacterial viruses unable to infect eukaryotic cells, constitute a serious alternative to antibiotic therapy of bacterial infections [ 5 ]. (biomedcentral.com)

Anatomy1

Diseases1

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes1

ALCAM30

CD1668

Endothelium10

Cytokine-activated2

Antigen-prese1

Differentiation3

Mediates1

Cytokines4

Protein10

Modulates1

Biol1

Inflammatory7

Ligand4

Human endothelial cells1

Recruitment5

Proteins7

Antigens2

Monocytes2

Upregulation3

Interactions2

Intercellular2

Signaling pathway1

Binds2

Mediators2

Induces2

Tumor cells2

Cancer stem cell m1

Signal Transduction1

Tissues3

Fluorescence3

Melanoma1

Synovial4

Receptors3

Membrane2

Monocyte1

Soluble5

Platelet2

Accompanied by in1

Molecular3

Microvascular endothelial cells1

Endothelial layer2

Neutrophils2

Hematopoietic3

Monoclonal2

Inhibition1

Interaction3

Stem cells7

Oral squamous cell carc1

CD182

Vitro3

Macrophages1

Immune5

Marker1

Surface4

Expression5

Deficiency5

Bone1

Targets1

Pathways1

Bacterial infections1