The side chains of these amino acids display considerable chemical diversity, giving rise to an enormous number of protein structures such as enzymes that carry out biochemical reactions and carrier proteins such as hemoglobin, which transport oxygen to our tissues. (scitechdaily.com)
Prompted by studies of a short viral protein that spans cell membranes and causes tumors, the Yale team devised a series of synthetic membrane proteins just 26 amino acids long. (scitechdaily.com)
Remarkably, they report, they have even built these proteins from sequences of just two amino acids with very similar side chains. (scitechdaily.com)
The specificity of the proteins is determined by the sequences outside the repeats themselves. (embl.de)
We chose proteins that include amino and carboxyl extensions as well as proteins that are made up entirely of WD-repeats. (embl.de)
Adults with no caries neutralize these acids better than adults with severe caries, and their parotid saliva possesses longer, less fragmented basic proline rich proteins, BPRPs (Ayad et al. (umich.edu)
All known human proteins were clustered according to the relative frequencies of their amino acids starting from the UniProtKB/Swiss-Prot reference database and making use of hierarchical cluster analysis. (unito.it)
Many extracellular proteins with highly specific and repetitive sequences (keratins, collagens etc.) cluster clearly confirming the accuracy of the clustering method. (unito.it)
Proteins with a more complex structure with multiple domains (catalytic, extracellular, transmembrane etc.), even if classified very similar according to sequence similarity and function (aquaporins, cadherins, steroid 5-alpha reductase etc.) showed different clustering according to amino acid content. (unito.it)
Minimotifs are modular contiguous peptide sequences in proteins that are important for posttranslational modifications, binding to other molecules, and trafficking to specific subcellular compartments. (stanford.edu)
Some molecular functions of proteins in cellular pathways can be predicted from minimotif consensus sequences identified through experimentation. (stanford.edu)
All translated proteins end with a carboxylic acid commonly called the C-terminus. (stanford.edu)
By searching for new anchored sequences on the last 10 amino acids of proteins in the human proteome with lengths between 3-10 residues and up to 5 degenerate positions in the consensus sequences, we have identified new consensus sequences that predict instances in the majority of human genes. (stanford.edu)
Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments. (umassmed.edu)
The researchers expressed these repetitive proteins in E. coli (on a plasmid), extracted them, and mixed them with an FDA-approved surfactant used in the cosmetics industry, called sodium dodecylbenzene sulfonate. (readcodon.com)
The predicted structures of the Gimap proteins show common sequences and motifs, such as GTP-binding domains in the N-terminal half, but with differing C-terminal ends [ 2 , 3 ]. (hindawi.com)
The fact that the Gimap genes are located together in a tight cluster on RNO4 (and in conserved synteny with many other species), combined with their sequence similarities, suggests the possibility that the proteins carry out similar function. (hindawi.com)
Nature has refined structural proteins to perform defined functions through the association of specific amino acid sequences in various combinations, creating diverse materials with remarkable properties. (springeropen.com)
Peptide sequences3
For this study, the researchers designed peptide sequences, similar to elastin, that contain repeated strings of amino acids: valine-proline-glycine-lysine-glycine. (readcodon.com)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)
Nucleic Acids Re1
The work was published in Nucleic Acids Research this week and was authored by Layla Schuster and Christopher Reisch at the University of Florida in Gainesville. (readcodon.com)
Single nucleotide po1
Nucleotide sequences contained numerous single nucleotide polymorphisms (SNPs), including amino acid to stop codon conversions and crossover events that deleted portions of PRB1 and/or PRB2. (umich.edu)
Genes8
FAM89A is neighboring the genes TRIM67 (Tripartite Motif Containing 67), located downstream of FAM89A on the plus strand of chromosome 1, and ARV1 (ARV1 Homolog, Fatty Acid Homeostasis Modulator), located upstream of FAM89A on the plus strand of chromosome 1. (wikipedia.org)
The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)
A total of 1,194 potential lactic acid bacterium (LAB) isolates were tentatively grouped and identified by repetitive element sequence-based PCR, followed by sequence-based identification using 16S rRNA and pheS genes from a selection of genotypically unique LAB isolates. (who.int)
Because longer genes encode longer BPRPs, we investigated the possibility of exon sequencing BPRP alleles encoded by PRB1 and PRB2. (umich.edu)
Of the 16 genes sequenced, 2 had a crossover between PRB1 and PRB2 and 4 had stop mutations. (umich.edu)
Generally, the challenges in inherited retinal diseases, in addition to difficult-to-sequence genes, is the large number of overlapping disease-causing genes and phenotypes. (blueprintgenetics.com)
Our aim was to clone and sequence the cDNA of the BB diabetes prone (DP) and diabetes resistant (DR) alleles of all seven Gimap genes in the congenic DR. lyp rat line with 2 Mb of BB DP DNA introgressed onto the DR genetic background. (hindawi.com)
The first stage involved identifying, in unrelated subjects showing linkage to 2q24-q33, genetic variants in exons and flanking sequence within candidate genes and comparing the frequency of the variants between autistic and unrelated nonautistic subjects. (neurotransmitter.net)
Motifs5
This review aims to link recent molecular data, often translated into amino acid sequences and predicted three dimensional structural motifs, to known mechanical properties. (bioone.org)
WD-40 repeats (also known as WD or beta-transducin repeats) are short ~40 amino acid motifs, often terminating in a Trp-Asp (W-D) dipeptide. (embl.de)
Silk-elastin-like polymers (SELPs) are protein-based polymers composed of repetitive amino acid sequence motifs found in silk fibroin (GAGAGS) and mammalian elastin (VPGVG). (springeropen.com)
These have served as a source of inspiration for a new class of bio-engineered materials, the protein-based polymers (PBPs), founded on conservative amino acid motifs found in nature. (springeropen.com)
Most commonly, they are based on repetitions of the silk fibroin amino acid motif GAGAGS and the mammalian elastin conserved motif VPGVG, with the proportions, number and sequence of these repeated motifs governing the properties of the polymer. (springeropen.com)
Exon2
Blueprint Genetics' new laboratory assay, powered by customized oligo design sequencing with Illumina NovaSeq and combined with an updated proprietary analysis pipeline, provides a high diagnostic rate in internal clinical validation studies, even with challenging regions such as the repetitive central region of the ORF15 exon. (blueprintgenetics.com)
Protein FAM89A (family with sequence similarity 89, member A) is a protein which in humans is encoded by the FAM89A gene. (wikipedia.org)
The FAM89A protein is 184 amino acids in length, and it has a predicted molecular mass of 18.6kDa and a predicted isoelectric point of 5.64. (wikipedia.org)
FAM89A contains a conserved leucine-rich adapter protein domain (LURAP) called PF14854, located at amino acid positions 84-122. (wikipedia.org)
The wide array of protein function, upon which all life depends, is due to the specific sequences of large groups of amino acids that typically number in the hundreds or thousands. (scitechdaily.com)
The paper focuses on the development of a software tool for protein clustering according to their amino acid content. (unito.it)
Availability of essential amino acids according to local conditions (starvation, low or high oxygen, cell cycle phase etc.) may be a limiting factor in protein synthesis, whatever the mRNA level. (unito.it)
Trueman SF, Mandon EC, Gilmore R. Translocation channel gating kinetics balances protein translocation efficiency with signal sequence recognition fidelity. (umassmed.edu)
Use of synthetic signal sequences to explore the protein export machinery. (umassmed.edu)
When these repetitive protein sequences are expressed within a cell, such as E. coli , the lysine amino acid in the fourth position is protonated. (readcodon.com)
The proposed method is novel because it works independently of protein sequence databases and without any prior knowledge about modifica- tions. (lu.se)
The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)
PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
Fatty acids2
High concentration of phytosterols and unsaturated fatty acids. (medicals-cosmetics.com)
When the biodiesel is produced by microalgae, the content of lipids and types of fatty acids in cells are the important considerations [6]. (researchsquare.com)
Elastin1
In the present study we have designed and synthesised four novel SELPs in which the most commonly used elastin pentamer sequence VPGVG is altered to VPAVG. (springeropen.com)
The avenins of oats have lower sequence similarity and a low toxicity profile. (karger.com)
Repeats1
FAM89A shows five periodic repeats of leucine residues at every seventh amino acid position at positions 81-115, which is characteristic of its predicted leucine zipper structural motif. (wikipedia.org)
Isolates1
To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
Sequence reads were assembled to the human reference genome using Burrows-Wheeler Aligner software (BWA) and the Genome Analysis Tool Kit (GATK) which identifies genomic variants. (umich.edu)
Additionally, we have developed a custom Sanger sequencing method to confirm variants specifically in the ORF15 region. (blueprintgenetics.com)
Similarities1
Results were compared to those based on sequence similarities. (unito.it)
Periodic1
2002 ) while the periodic inclusion of the elastomeric sequence reduces the overall crystallinity of the system and increases its flexibility and aqueous solubility (Cappello et al. (springeropen.com)
Large1
Irrespective of the LAB biodiversity, a large majority of the sugar and amino acid metabolites were detected in all sourdough samples. (who.int)
Gene2
Technically, the ORF15 region of the RPGR gene is very challenging to sequence. (blueprintgenetics.com)
The combination of these sequencing and bioinformatic innovations represents one of the best strategies for RPGR gene diagnostics available on the market," Alastalo continues. (blueprintgenetics.com)
Content2
Unfortunately, precise genomic structure could not be determined with certainty due to high GC content and repetitive elements which negatively impacted read mapping accuracy. (umich.edu)
In our case clustering by sequence and amino acid content overlaps. (unito.it)
Cell1
A simple acid treatment allowed for cell disruption and the obtention of relative pure SELP in one-step, with ammonium sulphate precipitation being subsequently used to enable improved purity. (springeropen.com)
Methods3
Methods: All exons of PRB1 and PRB2 from 8 healthy adults were sequenced using an Illumina HiSeq 2000 (100 bp paired sequencing readouts with TruSeq™ Exome Enrichment Kit). (umich.edu)
Exploring the interactions between signal sequences and E. coli SRP by two distinct and complementary crosslinking methods. (umassmed.edu)
As a part is copied by PCR, the primers produce overhang sequences that match the adjacent parts, enabling 4-part plasmids to be created using simple genetic cloning methods. (readcodon.com)
FAM89A has three predicted phosphorylation sites located at amino acid positions 30, 32, and 168 that are conserved in distant orthologs. (wikipedia.org)
BPRPs bind to streptococcal adhesion antigen I/II (antigen c) where the longer BPRPs would neutralize acids better at sites of acid production. (umich.edu)
Short1
Many short functional sequences (minimotifs) are located on or immediately proximal to the C-terminus. (stanford.edu)
Present1
We present a detailed sequence analysis of the 10 formins (ForA to J) identified in the genome of the social amoeba Dictyostelium discoideum . (biomedcentral.com)
Display1
Despite this extraordinary simplicity, a tiny fraction of these sequences display biologic activity and can be isolated by genetic selection, said the study's senior author Dr. Daniel DiMaio, the Waldemar Von Zedtwitz Professor of Genetics and deputy director of Yale Cancer Center. (scitechdaily.com)
Match1
These approaches depend on sequence databases that are used by the engines to match real spectra to theoretical in silico spectra. (lu.se)
Trueman SF, Mandon EC, Gilmore R. A gating motif in the translocation channel sets the hydrophobicity threshold for signal sequence function. (umassmed.edu)