• The objectives of this study were to construct a normalized cDNA library for efficient EST analysis, to generate thousands of ESTs, and to analyze the ESTs for microsatellites and potential single nucleotide polymorphisms (SNPs). (nih.gov)
  • A total of 35 microsatellites were identified from the ESTs, with 18 having sufficient flanking sequences for primer design. (nih.gov)
  • A total of 5,542 ESTs were generated representing 4,688 unique sequences. (nih.gov)
  • In most microarray experiments, a significant fraction of the differentially expressed mRNAs identified correspond to expressed sequence tags (ESTs) and are generally discarded from further analyses. (elsevierpure.com)
  • We used careful bioinformatics analyses to characterize those ESTs that were found to be highly overexpressed in a series of pancreatic adenocarcinomas. (elsevierpure.com)
  • The GeneExpress® software system Fold Change Analysis Tool was used and 60 ESTs were identified that were expressed at levels at least 3-fold greater in the pancreatic cancers as compared to normal tissues. (elsevierpure.com)
  • Searches against the human genomic sequence and comparative genomic analysis of human and mouse genomes was carried out using basic local alignment search tools (BLAST), BLASTN, and BLASTX, for identifying protein coding genes corresponding to the ESTs. (elsevierpure.com)
  • Analysis of 20,257 high-quality ESTs yielded 6372 unique genes comprised of 3059 tentative consensus (TC) sequences and 3313 singletons (available at http://aksoylab.yale.edu ). (umd.edu)
  • Progress towards this goal requires the development of genomic resources for catfish, including expressed sequence tags (ESTs). (auburn.edu)
  • In the context of catfish immune research, ESTs provide a foundation for both research on individual immune-related genes and microarray-based transcriptome analysis following infection. (auburn.edu)
  • In silico analysis of the Medicago truncatula gene index release 8.0 at The Institute for Genomic Research identified about 530 putatively symbiosis-specific tentative consensus sequences (TCs) clustered from 2,700 expressed sequence tags (ESTs) derived solely from Sinorhizobium meliloti-inoculated root and nodule tissues. (usda.gov)
  • A total of 81 ESTs were upregulated in both effective and ineffective nodules, and 78% of these ESTs were identified by in silico analysis as symbiosis-specific. (usda.gov)
  • Some of the ESTs in this collection have low sequence similarity with those in Genbank suggesting that they may encode functions that may contribute to Thellungiella's high degree of stress tolerance when compared with Arabidopsis. (mcmaster.ca)
  • cDNAs averaged 500-600 bp in length and were treated as expressed sequence tags (ESTs). (mpg.de)
  • Clustering analysis of 805 random cDNAs resulted in 179 and 155 different ESTs for the oxic and anoxic zones respectively. (mpg.de)
  • As a first step in pursuing this research, we report the generation and analysis of expressed sequence tags (ESTs) from the male accessory gland of H. erato and H. melpomene, species representative of the two mating systems present in the genus Heliconius. (ku.edu)
  • Results: We successfully sequenced 933 ESTs clustering into 371 unigenes from H. erato and 1033 ESTs clustering into 340 unigenes from H. melpomene. (ku.edu)
  • Conclusion: This collection of ESTs will serve as the foundation for the future identification and evolutionary analysis of male reproductive proteins in Heliconius butterflies. (ku.edu)
  • We identified 719 unique ESTs from a total of 1412 sequences so generated. (biomedcentral.com)
  • Results We describe a bioinformatics pipeline known as FunnyBase that has been used to store, annotate, and analyze 40,363 expressed sequence tags (ESTs) from the heart and liver of the fish, Fundulus heteroclitus. (umsystem.edu)
  • Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. (zju.edu.cn)
  • Analysis of ESTs. (zju.edu.cn)
  • Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
  • Functional genomics has its foundation in the large-scale projects for collecting expressed sequence tags (ESTs) and for genome sequencing, begun in the 1980s. (biomedcentral.com)
  • Among the matched sequences, ESTs were identified from tissues other than berries or from berries at different developmental stages. (biomedcentral.com)
  • Additional MPSS signatures not matching to known grape ESTs can extend our knowledge of the V. vinifera transcriptome, particularly when these data are used to assist in annotation of whole genome sequences from Vitis vinifera . (biomedcentral.com)
  • For genetic characterization of rapid cell elongation in cotton fibers, ∼ 14,000 unique genes were assembled from 46,603 expressed sequence tags (ESTs) from developmentally staged fiber cDNAs of a cultivated diploid species (Gossypium arboreum L.). Conservatively, the fiber transcriptome represents 35-40% of the genes in the cotton genome. (edu.sa)
  • Here, we describe the isolation and characterization of expressed sequence tags (ESTs) isolated from a chicken pineal gland cDNA library. (westminster.ac.uk)
  • 6% of the ESTs matched neither GenBank cDNA sequences nor the newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences designated to be on the Z_random, while one matched a W chromosome sequence and could be useful in cataloguing functionally important genes on this sex chromosome. (westminster.ac.uk)
  • Additionally, single nucleotide polymorphisms (SNPs) were identified and validated in 10 ESTs that showed 98% or higher sequence similarity to known chicken genes. (westminster.ac.uk)
  • Loci for 9322 equine expressed sequence tags (ESTs) were predicted using the Comparative Mapping by Annotation and Sequence Similarity (Compass) strategy in order to evaluate the programme's ability to make accurate locus predictions in species with comparative gene maps. (uaeu.ac.ae)
  • The locations of 30 ESTs were assessed experimentally by RH mapping analysis to evaluate the accuracy of the Compass predictions. (uaeu.ac.ae)
  • Analysis of the results suggested the need to identify expressed orthologous sequences in order to generate more accurate predictions for ESTs. (uaeu.ac.ae)
  • We aimed to identify resistance gene analogs (RGAs) from peanut expressed sequence tags (ESTs) and to develop polymorphic markers. (jipb.net)
  • The protein sequences of 54 known R genes were used to identify homologs from peanut ESTs from public databases. (jipb.net)
  • A total of 1,053 ESTs corresponding to six different classes of known R genes were recovered, and assembled 156 contigs and 229 singletons as peanut-expressed RGAs. (jipb.net)
  • Expression analysis of the three yolk proteins indicates that all are detected in females, while only the yolk protein with similarity to lipases, is expressed in males. (umd.edu)
  • Among the 17 tissues, the highest similarity in gene expression patterns was between human brain and testis, based on DDD and clustering analysis. (karger.com)
  • One group of symbiosis-specific transcripts identified by the in silico analysis encodes eight genes with similarity to putative plant disease resistance (R) genes. (usda.gov)
  • Homology analysis showed the highest similarity to M2 muscarinic acetylcholine receptors and overall low homology to all other biogenic amine receptors. (aspetjournals.org)
  • Primary annotations based on sequence similarity are linked to networks of systematic annotation in Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and can be queried and computationally utilized in downstream analyses. (umsystem.edu)
  • These genome resources provide the material basis for future microarray development, marker validation, and genetic linkage and QTL analysis. (nih.gov)
  • We have shown that approximately 14% of the newly sequencedexpressed sequence tags map completely or partially to genomic regions where there are no annotated genes orsplice variants and therefore, imply that these are novel genetic structures. (sfu.ca)
  • We report the first study in which polymorphic expressed sequence tag-simple sequence repeat (EST-SSR) markers derived from Medicago, cowpea and soybean were used to assess the genetic diversity of the Lespedeza germplasm collection and clarify its phylogenetic relationship with the genus Kummerowia. (usda.gov)
  • Genetic and molecular analyses have led to the identification, cloning and characterization of a few genes that contribute to parasitic fitness in the pathogens. (sisef.it)
  • Mendelian genetic analysis of the pathogens is therefore easy and the heritability of any scorable phenotypic or genotypic trait can be assessed rapidly. (sisef.it)
  • Molecular and omic approaches, however, represent technological advances that should allow rapid developments in the genetic analysis of elms and bark beetle vectors of the disease. (sisef.it)
  • This reference genome sequence is a historical landmark of catfish research as it opens the real first step of the long march toward genetic enhancement. (syr.edu)
  • In this study, expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using transcriptome mining for future genetic and functional studies. (bibbase.org)
  • This deep EST-SNP analysis provided important information on the genetic and genomic diversity of the olive complex, opening new opportunities to detect gene polymorphisms with potential functional and evolutionary roles, and to apply them in genomics-assisted breeding, highlighting the importance of olive germplasm conservation. (unboundmedicine.com)
  • Particularly in the past decade, genetics and genomics have been instrumental in the conservation biology of these cryptic vertebrates, enabling work ranging from the identification of populations subject to trade and exploitation, to the identification of cryptic lineages harboring critical genetic variation, to the analysis of genes controlling key life history traits. (deepdyve.com)
  • In this review, we highlight some of the most important ways that genetic analyses have brought new insights to the conservation of amphibians and reptiles. (deepdyve.com)
  • Co-dominant simple sequence repeat (SSRs) are popular DNA markers, widely applied in oil palm genetic studies, especially in genetic mapping, marker-trait association, diversity analysis and detecting illegitimacy. (mpob.gov.my)
  • OPSRI is a well-structured database that can expedite marker development and reduce redundancy in the on-going efforts at developing DNA markers for genetic analysis of oil palm. (mpob.gov.my)
  • Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers [J]. J Integr Plant Biol. (jipb.net)
  • A genetic linkage map spanning 582 centimorgans (cM) was constructed with EST-SSR (simple sequence repeat markers derived from expressed sequence tags) and used to identify QTL for herbage dry weight (DW) and seed yield per plant (SdYP), and their key component traits. (massey.ac.nz)
  • Cloning and characterization of genes specifically expressed during infection stages in the rice blast fungus. (zju.edu.cn)
  • Diversity characterization and association analysis of agronomic traits in a Chinese peanut ( Arachis hypogaea L.) mini-core collection [J]. J Integr Plant Biol. (jipb.net)
  • Using this approach, we have generated a large amount of data forexpressed sequence tags, which provides an opportunity for the discovery of putative novel transcripts andalternative splice variants that could be developmentally specific to the first larval stage. (sfu.ca)
  • A bioinformatics analysis identified 2087 unique and putative microsatellites, from which 96 novel microsatellite markers were developed. (bibbase.org)
  • In addition, we have provided a way to explore the expression of putative M. truncatula transporter genes under stress treatments.Conclusions: In summary, the MTDB enables the exploration and comparative analysis of putative transporters in M. truncatula. (wustl.edu)
  • Initial microarray analyses in channel catfish and blue catfish liver following an infection with E. ictaluri captured a high number of unique, differentially expressed transcripts and indicated the strong upregulation of several pathways involved in the inflammatory immune response. (auburn.edu)
  • Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
  • These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)
  • A total of 6,528 clones were sequenced from this library generating 5,542 high-quality EST sequences. (nih.gov)
  • Almost 2,000 expressed sequence tag (EST) cDNA clones were sequenced to analyze gene expression in control and Cd-treated leaf trichomes. (uni-bayreuth.de)
  • Gene expression was studied by cDNA microarrray analyses using 4608 unique clones from liver-derived expressed sequence tag (EST) libraries fortified with clones of known liver genes representing ∼4000 genes. (houstonmethodist.org)
  • Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. (ejbiotechnology.info)
  • With genome or EST sequences in hand, microarrays or macroarrays can be constructed to analyze gene-expression profiles at the genome-wide level over a range of conditions. (biomedcentral.com)
  • For example, our group has examined the genes regulated by the Zap1p transcription factor in S. cerevisiae using DNA microarrays and promoter motif analysis [ 1 ] (Figure 1 ). (biomedcentral.com)
  • The small genomes of the DED fungi also facilitate genomic analyses. (sisef.it)
  • Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)
  • DAVID was used to perform the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis for the different expression genes (DEGs). (medscimonit.com)
  • Although genomics has only recently emerged as part of this conservation tool kit, several large-scale data sources, including full genomes, expressed sequence tags, and transcriptomes, are providing new opportunities to identify key genes, quantify landscape effects, and manage captive breeding stocks of at-risk species. (deepdyve.com)
  • However, when applying this concept to genomes of multicellular organisms such as mammals, one has to deal with a large number of false positive predictions since many transcription factor genes are only expressed in specific tissues or cell types. (lu.se)
  • Data imported during this process includes the Gene ID, Genbank/RefSeq nucleotide and protein sequence identifiers, HomoloGene group IDs and Ensembl Gene, Transcript and Protein IDs. (wikipedia.org)
  • Gene expression patterns in human liver cells exposed to tetrachloroethylene and it's metabolite using microarray analysis. (cdc.gov)
  • However, large-scale expressed sequence tag (EST) resources have been lacking for this species. (nih.gov)
  • Phylogenetic analysis partitioned 44 Lespedeza accessions into three main groups, some of which were species-specific and eight subgroups. (usda.gov)
  • ABSTRACT Temperature responses of nitrate reductase (NR) were studied in the psychrophilic unicellular alga, Koliella antarctica, and in the mesophilic species, Chlorella sorokiniana. (academicconcepts.net)
  • In both species only a third of accessory gland unigenes were also found among genes expressed in wing tissue. (ku.edu)
  • abstract = "Papaver species are known to produce a large variety of benzylisoquinoline alkaloids, with each species exhibiting a specific alkaloid profile, but only a few genes involved in the biosynthesis or regulation of these complex pathways are known so far. (wustl.edu)
  • abstract = "Catfish is the primary aquaculture species in the United States. (syr.edu)
  • article{jin_development_2015, title = {Development of 23 novel polymorphic {EST}-{SSR} markers for the endangered relict conifer {Metasequoia} glyptostroboides}, volume = {3}, issn = {2168-0450}, url = {https://onlinelibrary.wiley.com/doi/abs/10.3732/apps.1500038}, doi = {10.3732/apps.1500038}, abstract = {Premise of the study: Metasequoia glyptostroboides is an endangered relict conifer species endemic to China. (bibbase.org)
  • Single nucleotide polymorphisms in the expressed sequence tag able to underline intra-species differentiation are not yet identified, beyond a few plastidial markers. (unboundmedicine.com)
  • abstract = "Background: Medicago truncatula has been chosen as a model species for genomic studies. (wustl.edu)
  • The obtained DNA sequence has shown reliable and clear species identification. (bvsalud.org)
  • Based on such considerable tools and resources, we have completed a high-quality reference genome sequence of channel catfish and its genome annotation. (syr.edu)
  • We constructed a full-length cDNA library of peanut ( Arachis hypogaea ) seed via a large number of sequences of expressed sequence tag (EST) and gene functional annotation, a lysophosphatidic acid acyltransferase gene, designated AhLPAT, and its genomic DNA sequence were isolated from peanut. (chinacrops.org)
  • Extensive annotations referring to each protein were generated, including basic protein function, expressed sequence tag (EST) mapping, genome locus, three-dimensional template prediction, transmembrane segment, and domain annotation. (wustl.edu)
  • Nuclear genome sequence and gene expression of an intracellular fungal endophyte stimulating the growth of cranberry plants. (umontreal.ca)
  • 2008. Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita . (hutton.ac.uk)
  • Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
  • To date, due to the high GC content and to the presence of telomeric repeats, the subtelomeric sequences are underrepresented in the genomic libraries and consequently their sequences are incomplete in the finished human genome sequence, and still much remains to be learned about subtelomere organization, evolution and function. (anthropogeny.org)
  • This data has been largely supplanted by the rat whole genome sequence. (wikipedia.org)
  • A total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. (hawaii.edu)
  • Twenty-eight simple sequence repeats (SSRs) were identified from 25 peanut expressed RGAs. (jipb.net)
  • A computer comparison of thousands of genes expressed in nodules with genes expressed in roots identified 530 genes that potentially are expressed during the symbiotic interaction. (usda.gov)
  • cDNA subtractive cloning of genes expressed during early stage of appressorium formation by Magnaporthe grisea . (zju.edu.cn)
  • Analysis of genes expressed during rice- Magnaporthe grisea interactions. (zju.edu.cn)
  • Here, we have described resources that may be useful in comparative and functional genomic analysis of genes expressed in an important organ, the pineal gland, in a model and agriculturally important organism. (westminster.ac.uk)
  • The Sequences data type is not a full list of either genomic, transcript or protein sequences, but rather mostly contains PCR primer sequences which define simple sequence length polymorphism (SSLP) and expressed sequence tag (EST) Markers. (wikipedia.org)
  • The differential gene expression data analysis was performed using GeneChip 4.0 software. (cdc.gov)
  • My research, as presented here, encompasses these two complementary approaches to EST research with in-depth studies of the catfish CC chemokine family and development and utilization of a high-density oligonucleotide microarray for expression analysis following E. ictaluri infection. (auburn.edu)
  • Gene expression analysis of these sequences in P. bracteatum, P. somniferum and P. somniferum 'Noscapine' exhibited 39 cDNAs showing differential expression coincident with morphine accumulation. (wustl.edu)
  • Microarray platforms have been developed for the analysis of genome expression. (syr.edu)
  • This type of "systems-level" analysis is critical to the understanding of patterns of gene expression that underlie biological processes. (umsystem.edu)
  • An integrated framework for cDNA library production, sequencing, quality control, expression data generation, and systems-level analysis is presented and utilized. (umsystem.edu)
  • The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea . (zju.edu.cn)
  • These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)
  • Expression profiling is the direct offspring of these sequencing projects. (biomedcentral.com)
  • The output of the method is conceptually similar to a possibly more familiar method called Serial Analysis of Gene Expression (SAGE) [ 8 ]. (biomedcentral.com)
  • In silico expression analysis revealed that rapidly elongating fiber cells exhibit significant metabolic activity, with the bulk of gene transcripts, represented by three major functional groups - cell wall structure and biogenesis, the cytoskeleton and energy/carbohydrate metabolism. (edu.sa)
  • The tissue specific expression analysis by using quantitative RT-PCR assays indicated that AhLPAT was ubiquitously expressed in root, stem, leaf, flower, gynophore and seed of peanut with the highest level in gynephore and seed. (chinacrops.org)
  • Cloning and Expression Analysis of Lysophosphatidic Acid Acyltransferase (LPAT) Encoding Gene in Peanut[J]. Acta Agron Sin, 2012, 38(02): 245-255. (chinacrops.org)
  • Targeted integration by homologous recombination enables in-situ tagging and replacement of genes in the marine microeukaryote Diplonema papillatum . (umontreal.ca)
  • This work alsodemonstrates the successful and efficient application of a next generation sequencing methodology.Results: We have generated over 30 million bases of novel expressed sequence tags from first larval stage wormsutilizing high-throughput sequencing technology. (sfu.ca)
  • Suchapplication of this new sequencing technique allows for high-throughput, genome-wide experimental verificationof known and novel transcripts. (sfu.ca)
  • Rapid progress in high-throughput sequencing technology and the simultaneous development of bioinformatics tools facilitated the progress of catfish genome studies. (syr.edu)
  • Crawford, Douglas L Abstract Background While studies of non-model organisms are critical for many research areas, such as evolution, development, and environmental biology, they present particular challenges for both experimental and computational genomic level research. (umsystem.edu)
  • Background: Expressed Sequence Tag (EST) sequences are generally single-strand, single-pass sequences, only 200-600 nucleotides long, contain errors resulting in frame shifts, and represent different parts of their parent cDNA. (umn.edu)
  • An expressed sequence tag (EST) library was constructed from mRNA obtained from fat bodies of A. mylitta larvae that had been challenged by infection with Escherichia coli cells. (biomedcentral.com)
  • This lack of increase contrasts with previous reports as does the mRNA length: this is 50% larger as confirmed by sequencing the 3' end. (embl.de)
  • Here we performed mRNA profiling using Massively Parallel Signature Sequencing (MPSS) and combined it with available Expressed Sequence Tag (EST) data. (biomedcentral.com)
  • METHODS: We conducted a test-negative case-control analysis among AI/AN persons aged 12 years presenting for care from January 1, 2021, through November 30, 2021, to evaluate the effectiveness of mRNA COVID-19 vaccines against COVID-19-associated outpatient visits and hospitalizations. (cdc.gov)
  • Closely matched sequences of genus Uromastyx were retrieved from NCBI in blast searches. (bvsalud.org)
  • The phylum-level affiliations of 93 16S rRNA sequences corresponded well with two taxonomically distinct EST patterns. (mpg.de)
  • The DNA of Uromastyx hardwickii was amplified and sequenced using 16S rRNA primer set. (bvsalud.org)
  • After trimming ambiguous bases, the obtained 16S rRNA fragment was 520 bp while 16S rRNA fragments aligned with closely matched sequence from NCBI comprised of 510 bp. (bvsalud.org)
  • Marker-trait validation confirmed markers pps0495 and pps0698 identified by QTL analysis to be potentially useful for selecting for fast leaf appearance and long LL, respectively, in perennial ryegrass. (massey.ac.nz)
  • Such sequences are useful primarily for researchers still using these markers for genotyping their animals and for distinguishing between markers of the same name. (wikipedia.org)
  • Genes for enzymes involved in organic acid synthesis (carbonic anhydrase, phosphoenolpyruvate carboxylase, malate dehydrogenase, and citrate synthase) along with genes involved in nitrogen metabolism (asparagine synthetase and glutamine synthetase) were co-expressed in effective nodules of M. truncatula. (usda.gov)
  • The aim of this integrated analysis is to trace changes in tardigrade metabolism and identify pathways responsible for their extreme resistance against physical stress. (biomedcentral.com)
  • The emphasis of this paper lies on the integrated analysis of metabolism during dehydration and the subsequent rehydration. (biomedcentral.com)
  • Here, we characterize epigenetic features of active promoter proximal regions and candidate distal regulatory elements to construct high-resolution chromatin interaction maps for maize via long-read chromatin interaction analysis by paired-end tag sequencing (ChIA-PET). (nature.com)
  • The maps indicate that chromatin loops are formed between regulatory elements, and that gene pairs between promoter proximal regions tend to be co-expressed. (nature.com)
  • These tag-based technologies, which do not require a priori knowledge of genomic sequence, are well-suited for transcriptional profiling. (biomedcentral.com)
  • As described in Materials and Methods, we constructed a cDNA library from fat body tissues of E. coli - challenged A. mylitta larvae and randomly sequenced a large number of inserts from the library. (biomedcentral.com)
  • Several hundred partial genomic DNA sequences as nucleotide-binding-site leucine-rich repeat (NBS-LRR) resistance genes ( R ) have been identified, but a small portion with expressed transcripts has been found. (jipb.net)
  • Among the unigenes, 94.1% encoded products that were most similar in amino acid sequence to Arabidopsis and 1.5% had no match with a member of the family Brassicaceae. (mcmaster.ca)
  • The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
  • Cluster analysis indicated the presence of 635 contigs and 4,053 singletons, generating a total of 4,688 unique sequences. (nih.gov)
  • Analysis revealed that a large proportion of NRSs could be unique to this microalga. (academicconcepts.net)
  • A stem expressed sequence tag database of ~1100 unique genes from Papaver somniferum was created. (wustl.edu)
  • The MPSS data presented here not only achieved a higher level of saturation than previous EST based analyses, but in doing so, expand the known set of transcripts of grape berries during the unique stage in development that immediately precedes the onset of ripening. (biomedcentral.com)
  • Furthermore, we partially sequenced the underrepresented subtelomeres of human chromosomes showing a common evolutionary origin. (anthropogeny.org)
  • Expressedsequence tags, which map across intron-exon boundaries (around 300), indicate possible alternative splice sites,while expressed sequence tags, which map near the ends of known transcripts (around 600), suggest extensionof the coding or untranslated regions. (sfu.ca)
  • Biotin labeled cRNA transcripts were synthesized, fragmented and hybridized to HuGeneFL GeneChip probe arrays representing more than 6800 human genes and expressed sequence tags. (cdc.gov)
  • The sequence depth of MPSS allowed us to capture and quantify almost all the transcripts at a specific stage in the development of the grape berry. (biomedcentral.com)
  • The number and relative abundance of transcripts from stage II grape berries was defined using Massively Parallel Signature Sequencing (MPSS). (biomedcentral.com)
  • In this article, we review recently developed methods that enable functional genomic analysis at the single-cell or multi-cell level. (ejbiotechnology.info)
  • Methods and Results: We collected 97,565 unigene sequences generated by 454 pyrosequencing. (bibbase.org)
  • A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity. (doe.gov)
  • DSN: CC37.NAMCS89.DRUG ABSTRACT This report provides documentation for users of the 1989 National Ambulatory Medical Care Survey (NAMCS) Micro-Data Tapes for Drug Mentions. (cdc.gov)
  • To facilitate analysis of these drug data, a data tape has been prepared which includes a separate record for each drug entered on each sample encounter form. (cdc.gov)
  • Our results demonstrated that phylogenetic analysis with morphological reexamination provides a more complete approach to classify accessions in a plant germplasm collection. (usda.gov)
  • We also identified several unknown proteins, some of which could have probable immune-related functions based on the results of the ProDom analysis. (biomedcentral.com)
  • Results: A dataset of 100 EST sequences, 50 with and 50 without, translation initiation sites, was created. (umn.edu)
  • RESULTS: Our analysis focused on 34 published studies on occupational health effects from MP exposure with half involving exposure to PVC and the other half a variety of other MPs to compare. (cdc.gov)
  • RESULTS: The analysis included 207 cases and 267 test-negative controls. (cdc.gov)
  • Phylogeny inference and principal coordinate analysis allowed to detect two distinct clusters, clearly separating wilds and guanchica samples from cultivated olives, meanwhile the structure analysis made possible to differentiate these three groups. (unboundmedicine.com)
  • We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)
  • During this process and the subsequent rehydration, metabolites were measured in a time series by GC-MS. Additionally expressed sequence tags are available, especially libraries generated from the active and inactive state. (biomedcentral.com)