Inositol polyphosphate multikinase (IPMK) is required for the biosynthesis of inositol phosphates (IPs) through the phosphorylation of multiple IP metabolites such as IP3 and IP4. (molcells.org)
Inositol phosphates (IP) are a group of inositol metabolites that contain multiple phosphates. (molcells.org)
The specificity of the site has been carefully evaluated using both natural and novel synthetic inositol phosphates. (ox.ac.uk)
Inositol phosphates (IPs) are phosphorylated derivatives of myo-inositol involved in the regulation of several cellular processes through their interaction with specific proteins. (bvsalud.org)
Phosphate13
This enzyme participates in inositol phosphate metabolism and phosphatidylinositol signaling system. (wikipedia.org)
IP6 can be further metabolized by the inositol-hexakisphosphate kinases (IP6Ks), which add a phosphate at the 5-position to produce 5-diphosphoinositol pentakisphosphate (5-PP-IP5,5-IP7). (molcells.org)
D-Ins(2,4,5)P3 was the only other phosphate to show relatively high affinity (KD 1500 nM). (ox.ac.uk)
We incubated these scyllo-inositol polyphosphates with these kinases and ATP as the phosphate donor. (bvsalud.org)
All scyllo-inositol polyphosphates are substrates for both the kinases but they are weaker than the corresponding myo-inositol phosphate. (bvsalud.org)
BACKGROUND: Myo-Inositol Phosphate Synthase (MIP) catalyzes the conversion of glucose 6- phosphate into inositol phosphate, an essential nutrient and cell signaling molecule. (bvsalud.org)
To provide further insight into the role of MIP in intercellular milieus, we tested the hypothesis that MIP may function as a growth factor, synthesizing inositol phosphate in intercellular locations requiring, but lacking ability to produce or transport adequate quantities of the cell-cell communicator. (bvsalud.org)
CONCLUSIONS: Co-culturing experiments, designed to test a probable function for MIP secreted in extracellular vesicles, uncovered previously unknown functions for the enzyme and advanced current knowledge concerning spatial control of inositol phosphate biosynthesis. (bvsalud.org)
Most importantly, resulting data identified an extracellular vesicle (a non-viral vector) that is capable of synthesizing and transporting inositol phosphate, a biological activity that can be used to enhance specificity of current inositol phosphate therapeutics. (bvsalud.org)
Here, we combined reverse genetics and liquid chromatography coupled to mass spectrometry (LC-MS) to dissect the inositol phosphate biosynthetic pathway and its metabolic intermediates in the main life cycle stages (epimastigotes, cell-derived trypomastigotes, and amastigotes) of Trypanosoma cruzi, the etiologic agent of Chagas disease. (bvsalud.org)
Phosphatidylinositols are acidic (anionic) phospholipids that consist of a phosphatidic acid backbone, linked via the phosphate group to inositol (hexahydroxycyclohexane). (ymdb.ca)
Phosphatidylinositol can be phosphorylated by a number of different kinases that place the phosphate moiety on positions 4 and 5 of the inositol ring, although position 3 can also be phosphorylated by a specific kinase. (ymdb.ca)
Seven different isomers are known, but the most important in both quantitative and biological terms are phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. (ymdb.ca)
Polyphosphate4
However, the physiological role of these inositol polyphosphate isomers in the heart remains to be elucidated, because, from the time course, they appear to have no acute intracellular second messenger function. (aspetjournals.org)
Increased inositol polyphosphate turnover may be involved in the mechanism(s) whereby alpha 1-adrenoceptor stimulation produces an increase in myocardial force of contraction. (aspetjournals.org)
The phosphotransfer from ATP to either inositol 1,3,4-trisphosphate or inositol 3,4,5,6-tetrakisphosphate appears to be freely reversible to the extent that the enzyme can act like an inositol polyphosphate phosphatase in the presence of ADP. (enzyme-database.org)
Insights into the biological functions of IPs have come from genetic studies in yeast and by manipulating inositol polyphosphate multikinase (IPMK) expression in mammalian cells. (molcells.org)
Synthesis5
Biosynthesis of IP metabolites begins with the synthesis of inositol 1,4,5-trisphosphate (InsP 3 , IP3), which is produced by phospholipid-specific phospholipase C (PLC). (molcells.org)
Synthesis, Fluorine-18 Radiolabeling, and Biological Evaluation of N-((E)-4-Fluorobut-2-en-1-yl)-2β-carbomethoxy-3β-(4′-halophenyl)nortropanes: Candidate Radioligands for In Vivo Imaging of the Brain Dopamine Transporter with Positron Emission Tomography. (acs.org)
IP6K and PPIP5K are two kinases involved in the synthesis of inositol pyrophosphates. (bvsalud.org)
Synthetic analogs or mimics are necessary to understand the substrate specificity of these enzymes and to find molecules that can alter inositol pyrophosphate synthesis. (bvsalud.org)
The synthesis of PI involves CDP-activated 1,2-diacylglycerol condensation with myo-inositol. (ymdb.ca)
Enzyme8
The systematic name of this enzyme class is ATP:1D-myo-inositol-3,4,5,6-tetrakisphosphate 1-phosphotransferase. (wikipedia.org)
This enzyme also phosphorylates Ins(1,3,4) P 3 on O-5 and O-6. (enzyme-database.org)
We tracked enzyme activity by measuring the amount of radiolabeled scyllo-inositol pyrophosphate product formed and the amount of ATP consumed. (bvsalud.org)
This idea was experimentally challenged, utilizing a Saccharomyces cerevisiae inositol auxotroph with no MIP enzyme, permeable membranes with a 0.4 µm pore size, and cellular supernatants as external sources of inositol isolated from S. cerevisiae cells containing either wild-type enzyme (Wt-MIP), no MIP enzyme, auxotroph (Aux), or a green fluorescent protein (GFP) tagged reporter enzyme (MIP- GFP) in co- culturing experiments. (bvsalud.org)
These findings revealed previously unknown functions, suggesting that the enzyme can: (1) move into and out of intercellular space, (2) traverse cell walls, and (3) act as a growth factor to promote cellular proliferation of an inositol requiring cell. (bvsalud.org)
The enzyme is highly regioselective for the reduction of the endocyclic 1,2-double bond, and is stereoselective, producing only the 1 R -configured product. (enzyme-database.org)
The ability of the enzyme to use both O 2 and fumarate in cofactor reoxidation enables it to function under both aerobic and anaerobic conditions [5]. (enzyme-database.org)
The enzyme is a member of the succinate dehydrogenase/fumarate-reductase family of enzymes [5]. (enzyme-database.org)
Kinase3
The enzymes from animals and plants also have the activity of EC 2.7.1.159 , inositol-1,3,4-trisphosphate 5/6-kinase. (enzyme-database.org)
Yang, X. and Shears, S.B. Multitasking in signal transduction by a promiscuous human Ins(3,4,5,6) P 4 1-kinase/Ins(1,3,4) P 3 5/6-kinase. (enzyme-database.org)
a phosphatidylinositol 3-kinase (PI3K) to phosphorylate phosphatidylinositol 4,5-bisphosphate and produce phosphatidylinositol 3,4,5-trisphosphate, a major growth signal for activation of Akt kinase. (molcells.org)
Metabolism1
Incubations were carried out at 4 degrees and under these conditions HPLC analysis demonstrated that there was no significant metabolism of [3H]-(1,4,5)P3 in the presence or absence of ATP over 15 min. (ox.ac.uk)
In most organisms, the stereochemical form of the last is myo-D-inositol (with one axial hydroxyl in position 2 with the remainder equatorial. (ymdb.ca)
Membranes2
A very high density of stereospecific binding sites for inositol-(1,4,5)P3 have been identified in rat cerebellar membranes using [3H]inositol-(1,4,5)P3 and a rapid centrifugation step to separate free and bound ligand. (ox.ac.uk)
While most phospholipids have a saturated fatty acid on C-1 and an unsaturated fatty acid on C-2 of the glycerol backbone, the fatty acid distribution at the C-1 and C-2 positions of glycerol within phospholipids is continually in flux, owing to phospholipid degradation and the continuous phospholipid remodeling that occurs while these molecules are in membranes. (ymdb.ca)
Substrates1
In this context, we synthesized four scyllo-inositol polyphosphates-scyllo-IP5, scyllo-IP6, scyllo-IP7 and Bz-scyllo-IP5-from myo-inositol and studied their activity as substrates for mouse IP6K1 and the catalytic domain of VIP1, the budding yeast variant of PPIP5K. (bvsalud.org)
Mammalian1
Existence and alpha 1-adrenergic stimulation of inositol polyphosphates in mammalian heart. (aspetjournals.org)
Physiological1
PIs composed exclusively of non-phosphorylated inositol exhibit a net charge of -1 at physiological pH. (ymdb.ca)
Initiate1
These data are discussed in relation to a putative receptor on the endoplasmic reticulum by which Ins(1,4,5)P3 can initiate the release of bound Ca2+. (ox.ac.uk)
Glucose1
A most commonly occurring isomer of glucose used as a carbohydrate supplementation in case of nutrient deprivation and metabolic disorders, such as hypoglycemia. (drugbank.com)
Abstract1
2. {Advantage} of early selection through shortening the breeding cycle}, volume = {48}, issn = {0037-5349}, abstract = {Three main advantages from early selection in tree breeding have been recognized: 1. (upsc.se)
Concentrations1
Eukaryotic cells are surrounded by media containing free Ca 2+ concentrations that exceed 1 mM, but they manage to maintain a free Ca 2+ concentration in the cytoplasm that is four orders of magnitude lower. (schoolbag.info)
Affinity2
The reasons explaining their different tissue selec- translating, cannot be attributed to genomic activity (Weh- tivity and specific activity are still obscure and cannot be satisfactorily explained by the simple ␣- and -estrogen re- The naturally occurring steroid 17-estradiol, its ␣-isomer, ceptor affinity (Nadal et al. (drugstodaypdf.com)
The stereospecificity is very marked with the D-, DL- and L-isomers of Ins(1,4,5)P3 showing a 1:4:2000 ratio of affinity for the binding site. (ox.ac.uk)
Freely1
MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. (aspetjournals.org)
MicroM4
The concentration-response curves and the time course of the effects of phenylephrine (0.01-100 microM) on force of contraction and on inositol polyphosphates in isolated electrically stimulated perfused rat hearts (Langendorff technique) were studied. (aspetjournals.org)
1,4,5-IP3 (significant at 10 microM) and both IP4 isomers (significant at 1 microM) increased after alpha-adrenoceptor stimulation, whereas 1,3,4,5,6-IP5 and IP6 remained unaffected. (aspetjournals.org)
Phenylephrine had a concentration-dependent positive inotropic effect (significant at 1 microM). (aspetjournals.org)
All effects were antagonized by the alpha 1-adrenoceptor antagonist prazosin (0.1 microM), indicating receptor-mediated effects. (aspetjournals.org)
Concentration1
A nonradiometric high performance liquid chromatography metal dye detection technique was used to determine absolute concentration masses/changes of inositol polyphosphates in heart. (aspetjournals.org)
Cells1
2. A slow (0.5-4 Hz) membrane potential oscillation was observed in thalamocortical cells recorded in motor, sensory, associational and intralaminar thalamic nuclei. (researchgate.net)
Function1
Although we've reported preclinical function, aswell as clinical studies, that arm ATC with (a) anti-CD3 x anti-HER2 BiAb (HER2 BATs) for the treating breasts and prostate cancers (5, 6), and (b) anti-CD3 x anti-CD20 BiAb (Compact disc20 BATs) for the procedure non-Hodgkin's lymphoma (7) and MM in conjunction with stem cell transplantation, particular concentrating on to MM lines by CS1-BATs is not shown. (tech-strategy.org)
Conditions2
4. In control conditions, spectral analysis yielded typical results. (researchgate.net)
5. We conclude that in supine rest conditions, the oscillation of RR at low frequency is almost entirely accounted for by a baroreflex mechanism, since it is not produced in the absence of a 0.1 Hz pressure oscillation. (researchgate.net)
Cell3
PLCs are activated by external stimuli such as growth factors to cleave phosphatidylinositol 4,5-bisphosphate and produce IP3, which is then released from the cell membrane into the cytosol. (molcells.org)
The ER was originally considered to be the sole dynamic Ca 2+ regulator in the cell, but it has now become clear that the nucleus, the mitochondria, the Golgi apparatus, the endosomes/lysosomes, and the secretory vesicles also play fundamental roles (Fig. 1). (schoolbag.info)
In conclusion, for the first time the existence of 1,3,4,5,6-IP5 and IP6 was observed in the heart. (aspetjournals.org)
1. Parameters derived from frequency-domain analysis of heart period and blood pressure variability are gaining increasing importance in clinical practice. (researchgate.net)
0.5) and a negative phase shift (-65.1 +/- 18 deg) between RR and systolic pressure, which indicates a 1-2 s lag in heart period changes in relation to pressure. (researchgate.net)
8. These results demonstrate that the majority of thalamocortical neurones are endowed with electrophysiological properties allowing them to oscillate at 0.5-4 Hz, if they have a membrane potential more negative than -65 mV and a high input resistance. (researchgate.net)
Department1
31784961 inviting quotations for purchase of pipette controller for department of biochemistry at aiims raipur.1 pipette controller : a. electronic pipette controller designed for use with serological and volumetric pipettes. (chhattisgarhtenders.net)
Presence1
It can also catalyse an isomerization between Ins(1,3,4,5) P 4 and Ins(1,3,4,6) P 4 in the presence of ADP. (enzyme-database.org)