2017 cells cultured. Medical search. Frequent questions

According to the report "Global Propofol Sales Market Report 2017" added by Market Research HUB, the global Propofol market is valued at USD XX million in 2016 and is expected to reach USD XX million by the end of 2022, growing at a CAGR of XX% between 2016 and 2022. (openpr.com)
WHO's Regional Office of the Eastern Mediterranean had conducted an assessment of these centres and influenza laboratories in 2015-2016, which revealed that some lack the necessary capacity for virus isolation in cell culture, and for antigenic characterization of human influenza viruses - capacities that are fundamental for early detection and recognition of influenza viruses of epidemic and pandemic potential. (who.int)

The evaluation of SPRP as a supplement showed that there was no statistical difference in cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. (springer.com)
We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)
The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)
2017. Divergent roles of p120-catenin isoforms linked to altered cell viability, proliferation, and invasiveness in carcinogen-induced rat skin tumors. . (oregonstate.edu)
Viability of cultured primary human skin cells treated with 1, 6-hexamethylene diisocyanate monomer and its oligomer isocyanurate. (cdc.gov)
Cell growth and viability were assessed after 7 and 10 days. (bvsalud.org)
001). Cell viability in all the groups was over 80%, except in group IV at day 7. (bvsalud.org)
Irradiation time of group I influenced positively the proliferation and viability of OD-21 cells in late cell culture period. (bvsalud.org)

2018). We further discovered that metabolic reprograming in neural stem cells irreversibly converts them into tumor stem cells initiating malignant overgrowth (Bonnay et al. (europa.eu)
18 years over three influenza seasons: (Southern Hemisphere 2017 and Northern Hemisphere 2017-18 and 2018-19). (cdc.gov)

Abstract Starting from mature vegetable compost, four bac- the utilization of these bacteria as cell factories for upgrading terial strains were selected using a lignin-rich medium. (lu.se)

The cell population was characterized by means of immunofluorescence and detection of PRKD1 gene mutations . (bvsalud.org)
The restriction digestion assay showed that the neoplastic cells possess PRKD1 gene mutations . (bvsalud.org)
Tumors' mutations can encode the seeds of their own destruction, in the form of immunogenic peptides recognized by T cells. (the-scientist.com)
which means both copies of the gene in each cell have mutations. (medlineplus.gov)

The global cell culture market size was evaluated at $5.55 billion in 2022 and is slated to hit $8.75 billion by the end of 2030 with a CAGR of nearly 8.37% between 2023 and 2030. (zionmarketresearch.com)

Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. (mdpi.com)
Our group established the first laboratory cultures of phototrophic consortia that represent the only laboratory model system currently available for the study of highly specific interactions between nonrelated bacteria. (dsmz.de)
Sincle cell genomic analysis of the bacteria constituting different types of phototrophic consortia indicate a long-term co-evolution of the partners. (dsmz.de)
One of the most interesting things about M. genitalium is that it doesn't have a cell wall and most bacteria do have a cell wall. (cdc.gov)

The ambr 15 software simultaneously acquires the BioProfile FLEX2 data set and processes it, for example, by performing complex in-run calculations of how much feed volume to automatically add during a run, which saves time by increasing the amount of consistent clone selection, media development, and culture parameter data that a scientist can generate per run. (biopharminternational.com)
Meet some of the people featured in the April 2017 issue of The Scientist . (the-scientist.com)
Crane L. New Scientist, May 31, 2017. (cdc.gov)

During the first reporting period, we were already able to improve the culture system by combining it with bio-engineered scaffold material, allowing for even better reconstitution of key developmental pathways (Lancaster et al. (europa.eu)
For the cell culture into the scaffold, it is necessary to establish certain conditions, such as the type of supplementation of the culture medium. (springer.com)
Therefore, the aim of this research was to evaluate the platelet-rich plasma (PRP) as a supplement and scaffold for Vero cell culture, in a one case report study. (springer.com)
SPRP was used as a supplement to the culture medium, and CPRP was utilized as a scaffold. (springer.com)
In the analysis of CPRP as a scaffold, it was verified that the cells adhered and spread over the biomaterial. (springer.com)
Therefore, in Vero cell cultures, the results suggest that SPRP and CPRP can be used as a supplement and scaffold, respectively. (springer.com)
MLO-Y4 osteocytes were cultured in Alvetex scaffold in either perfused or static conditions (using a perfusion plate or well inserts respectively) and the resulting conditioned medium was added to C2C12 myoblasts. (reprocell.com)
This is why some experiments are conducted using 3D cultures, which can be grown using scaffold-based or scaffold-free techniques. (integra-biosciences.com)
Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)
Scientists at the University of Cambridge have managed to create a structure resembling a mouse embryo in culture, using two types of stem cells - the body's 'master cells' - and a 3D scaffold on which they can grow. (cam.ac.uk)

Using human pluripotent stem cells, scientists engineered an amniotic sac-like structure, potentially useful for uncovering the elusive cellular signals that lead to infertility. (the-scientist.com)
Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular ardiomyoplasty. (springer.com)
Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
KOH dissolves keratin found in cellular material and freeing fungal hyphae and yeast cells. (slideshare.net)
For this purpose, a cellular model of senescent myocardial cells was set up and evaluated using colorimetric, fluorimetric, and immunometric techniques. (hindawi.com)
Relevant cellular senescence markers, such as X-gal staining, cell cycle regulator levels, and the percentage of cell cycle-arrested cells, were found to be reduced in the presence of naringenin. (hindawi.com)
Because aging has been demonstrated to be directly related to the occurrence of cardiac disorders, together, the data have prompted us to investigate the effects of Nar in a cellular model of aged myocardial cells. (hindawi.com)
Various cellular senescence hallmarks (the percentage of X-gal staining cells, the mRNA levels of the p16 and p21 cell cycle regulators, and the percentage of cell cycle-arrested cells) were investigated. (hindawi.com)

Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly (lactic acid) substrates. (springer.com)
The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. (springer.com)
The differentiation of endometrial stromal cells into decidual cells, termed decidualization, is an integral step in the establishment of pregnancy. (bioone.org)
Gene ontology and upstream regulator pathway analysis showed that WNK1 regulates cell migration, differentiation, and proliferation. (bioone.org)
Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (bvsalud.org)

2017). In addition, we could reconstruct even long-range interactions between distant parts of the human brain in the organoid system (Bagley et al. (europa.eu)
2017). This allowed us to reconstitute the long-range migration of human interneurons from the lower to the upper part of the brain. (europa.eu)
Various human and non-human cell lines were infected with a defined amount of ZIKV Polynesia strain. (wjgnet.com)
Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. (springer.com)
The case of cultured red blood cells currently being grown in a laboratory for future use in human transfusion raises questions about the ontological status of such products of modern biotechnology. (stir.ac.uk)
In a separate project, scientists from the University of Oxford cultured the human pancreatic cancer cell line PSN-1 over a period of seven days, in either static or perfused conditions, using the Alvetex perfusion plate . (reprocell.com)
The inhibitory effect of raloxifene, bazedoxifene, and acolbifene on LCA sulfonation was also observed in HepG2 human hepatocellular carcinoma cells. (aspetjournals.org)
Primary human endometrial stromal cells (HESCs) were subjected to small interfering RNA knockdown of WNK1 followed by in vitro decidualization. (bioone.org)
We are broadly interested in understanding how cells answer these questions and we address them by using multi-disciplinary approaches in yeast and human cell culture, such as ribosome profiling and computational analysis, biochemical techniques, and single-molecule fluorescence imaging. (nih.gov)
We think that it will be possible to mimic a lot of the developmental events occurring before 14 days using human embryonic and extra-embryonic stem cells using a similar approach to our technique using mouse stem cells," she says. (cam.ac.uk)
Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) [Yokota, T. et al . (rndsystems.com)
Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 Antibody. (rndsystems.com)
Recombinant Human IL‑12 (Catalog # 219-IL ) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). (rndsystems.com)
Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. (rndsystems.com)
Genotoxic Effects of Culture Media on Human Pluripotent Stem Cells. (ncbs.res.in)
While studying the progression of healthy cells into cancerous ones, researchers discover a way to engraft human blood cells into animals. (the-scientist.com)

Scientists fiddle with formulas to boost the growth of their stem cell cultures. (the-scientist.com)
These cells, derived from stem cell technology, have the potential to address the global shortage of donated blood. (stir.ac.uk)
However, it increases proliferation rates in some cell types (e.g. mesenchymal stem cells, osteosarcoma cells, HUVECs) which puts extra demands on nutrient supply and waste removal [1,2,3] . (reprocell.com)
In vitro expansion of mesenchymal stem cells using 3-D matrix derived from cardiac fibroblast. (reprocell.com)
Increase in the research on stem cells will proliferate the size of the global market in the years ahead. (zionmarketresearch.com)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)
Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR. (frontiersin.org)
A spontaneous cell line-like cell culture with high percentage of stem cells has been established from a DIG tumor for the first time. (frontiersin.org)
The condition polycystic kidney disease, also known as PKD, has been recreated using stem cells in a culture dish by. (thenakedscientists.com)
Once a mammalian egg has been fertilised by a sperm, it divides multiple times to generate a small, free-floating ball of stem cells. (cam.ac.uk)
The particular stem cells that will eventually make the future body, the embryonic stem cells (ESCs) cluster together inside the embryo towards one end: this stage of development is known as the blastocyst. (cam.ac.uk)
The other two types of stem cell in the blastocyst are the extra-embryonic trophoblast stem cells (TSCs), which will form the placenta, and primitive endoderm stem cells that will form the so-called yolk sac, ensuring that the foetus's organs develop properly and providing essential nutrients. (cam.ac.uk)
Professor Zernicka-Goetz and colleagues found a remarkable degree of communication between the two types of stem cell: in a sense, the cells are telling each other where in the embryo to place themselves. (cam.ac.uk)
The stem cells organise themselves, with ESCs at one end and TSCs at the other. (cam.ac.uk)
To do so, it would likely need the third form of stem cell, which would allow the development of the yolk sac, which provides nourishment for the embryo and within which a network of blood vessel develops. (cam.ac.uk)
Stem Cell Res. (ncbs.res.in)
Stem Cells. (bvsalud.org)
In the Dentistry field, stem cells derived situated in red and near-infrared region of electromagnetic from dental pulp have been isolated and investigated as spectrum. (bvsalud.org)
Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (bvsalud.org)

In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. (leica-microsystems.com)
Mammalian cell culture is one of the basic pillars of life sciences. (leica-microsystems.com)

Epifluorescence analysis of the primary culture revealed that the malignant epithelial cells were predominantly polygonal in shape and positive for cytokeratin 7 , vimentin , and S100. (bvsalud.org)
In the villi, this sheet contained numerous foramina ranging from 3 to 7 µm in diameter, through which lymphocytes, macrophages, basal extensions of epithelial cells and fat particles traversed. (karger.com)

From this the team identified five known brain cell types after three weeks in culture: oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes. (pennmedicine.org)
Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)

MRIs and other tests did not show any evidence of tumor or other abnormal cells in the non-tumor tissues used for the study. (pennmedicine.org)
Tumor tissue from a rare non-infantile 8-year-old female DIG patient was dissociated and digested using collagenase to establish live cultures. (frontiersin.org)
Using immunohistochemistry, the 3D cultures were shown to express markers as in the original DIG tumor tissue indicating that the spheroid cultures were able to maintain the heterogeneity found in the original tumor. (frontiersin.org)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
The establishment of primary cell culture derived from PLGA should be considered a useful tool for molecular analysis of this salivary gland tumor . (bvsalud.org)
Now we need to quantify more protein biomarkers, assess the tumor mutational landscape, and examine immune cell signatures, too. (the-scientist.com)
New monoclonal antibodies kill both cancer-promoting immunosuppressive cells and tumor cells in culture. (the-scientist.com)
Through similar mechanisms, amino acid depletion in culture and cytokine activity in the tumor microenvironment prompt cancer cells to metastasize. (the-scientist.com)

Conversion of Peripheral Blood NK Cells to a Decidual NK-like Phenotype by a Cocktail of Defined Factors The Journal of Immunology, 190:3939-3948 (2013). (bioind.com)
2017. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. . (oregonstate.edu)

In terms of revenue, the global cell culture market size was evaluated at nearly $5.55 billion in 2022 and is expected to reach $8.75 billion by 2030. (zionmarketresearch.com)
Market Research Hub includes new market research report "Global Active Pharmaceutical Ingredients Market Research Report 2017-2022 by Players, Regions, Product Types & Applications" to its huge collection of research reports. (openpr.com)

The systems have been shown to improve cell proliferation and specific protein expression beyond that seen for static 3D cultures [4,5,6,7] . (reprocell.com)
The levels of glucose and secreted lactate contained in the cell culture medium were monitored as indicators of metabolic depletion, while protein syntheses and cell proliferation were measured by expression of phosphorylated Ribosomal protein S6 and cyclin D1. (reprocell.com)
Results suggested that perfusion culture reduces hypoglycemia, anaerobic stress, and preserves protein synthesis and cell proliferation. (reprocell.com)
This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. (rndsystems.com)
Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. (rndsystems.com)

The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
The translation of the genetic code is fundamental to life and all cells have to determine which mRNAs get translated, how much they are translated, and where within the cell they are translated. (nih.gov)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. (wikipedia.org)
MEM has been used for cultivation of a wide variety of cells grown in monolayers. (sigmaaldrich.com)
Effects of three-dimensional culturing on osteosarcoma cells grown in a fibrous matrix: analyses of cell morphology, cell cycle, and apoptosis. (reprocell.com)
Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)
In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)
Meat, Poultry, & Seafood Alternatives: Plant-Based and Cell-Cultured Types is the go-to source for a complete understanding of U.S. consumer trends regarding plant-based meat products and the future market for cell-cultured (or cultivated, lab-grown) meat. (marketresearch.com)
This is a transmission electron microscopic (EM) image of Leishmania major amastigotes, which had been grown in a cell culture. (cdc.gov)

Vaping rates among teenagers in Maine doubled from 15.3% to 28.7% between 2017 and 2019, while Jake was in high school. (medscape.com)

Cell culture has contributed to the development of vaccines for many diseases. (wikipedia.org)
It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)
1 Today, cell culture has already helped countless discoveries, such as the development of vaccines against poliomyelitis, measles, mumps and other infectious diseases. (integra-biosciences.com)
Our work is therefore important for understanding what happens in the cells of patients with these diseases and finding novel targets for pharmaceuticals. (nih.gov)

The American embryologist Ross Granville Harrison developed the first in vitro cell culture technique at the very beginning of the twentieth century, when he successfully grew tissue fragments from frog embryos outside the body. (integra-biosciences.com)

The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Methods Cell. (bioind.com)

The morphological analysis, carried out on the third and fifth days, did not verify changes in the typical morphology of Vero cells cultured with SPRP. (springer.com)
This abrogated expression of the decidual marker genes, insulin like growth factor binding protein 1 ( IGFBP1 ) and prolactin ( PRL ), and prevented adoption of decidual cell morphology. (bioone.org)

Cells were analyzed 48 h post infection for the amount of intracellular and extracellular viral genomes and infectious viral particles by quantitative real-time PCR and virus titration assay. (wjgnet.com)
Although the analyzed cell lines released comparable amounts of viral genomes to the supernatant significant differences were found for the number of infectious viral particles. (wjgnet.com)

To identify cell culture models supportive for Zika virus (ZIKV) replication. (wjgnet.com)

Researchers use organoid cultures in unique ways to study health and disease. (the-scientist.com)

Sartorius Stedim Biotech combined the company's ambr 15 bioreactor system with the Nova BioProfile FLEX2 cell culture analyzer for laboratory experiments. (biopharminternational.com)
Lastly, laboratory cultures of phototrophic consortia also represent valuable model system for establishing suitable cryoconservation techniques for complex microbiomes. (dsmz.de)
Data obtained from FluNe t (http://www.who.int/influenza/gisrs_laboratory/flunet/en/ ), a global web-based tool for influenza virological surveil ance and provided by National Influenza Centres (NICs) in the WHO GISRS network. (who.int)

Establishment of a primary culture of polymorphous low grade adenocarcinoma cells. (bvsalud.org)

Although the LAT family plays important roles in the development and function of normal tissues, they are frequently increased in cancer cells ( 3 ). (spandidos-publications.com)
The roles of serotonin in cell adhesion and migration, and cytoskeletal remodeling. (ncbs.res.in)

Drawing on discussions related to classification and 'anchoring', we examine the contrasting discourses offered by our respondents on whether these blood cells are 'natural' or not and consider the impact that naming might have on both their future regulation and the eventual uptake of cultured red blood cells by society. (stir.ac.uk)

2 November 2017 - WHO conducted a 5-day workshop in Rabat, Morocco, on influenza virus identification and handling mechanisms to enhance the capacities of national influenza centres in the Eastern Mediterranean Region for timely and effective detection of seasonal and pandemic influenza. (who.int)
It focused on detection and identification of seasonal influenza virus sub-types, using cell culture and hemaglutination - steps that are essential for identifying viruses to make vaccines. (who.int)
As we observe the 100th anniversary of the 1918 In 2017, GISRS laboratories in the Western Pacific influenza pandemic, we are reminded of the Region tested nearly 800 000 specimens for influenza importance of preparedness for and adequate ( Fig. 1 ) . (who.int)
The epidemiological data to assist WHO in making recom- traditional method of influenza virus detection by isola- mendations on suitable virus strains for seasonal and tion in eggs or cell culture fol owed by antigenic typing pandemic influenza vaccines. (who.int)
Cell culture-based inactivated influenza vaccine (ccIIV4). (cdc.gov)
Overall vaccine efficacy 54.6% (95%CI 45.7, 62.1) against RT-PCR or culture) influenza- associated CDC-defined influenza-like illness. (cdc.gov)

The present study demonstrates that the topical application of TF extract improves skin inflammation in AD mice, and the mechanism for this effect appears to be related to the modulation of calcium channels and mast cell activation. (hindawi.com)
Mice engineered to overproduce the organelles involved in cell division spontaneously develop malignancies. (the-scientist.com)
Studies of mice and cell cultures have found that the vapor or extracts from vapes damage the chemical structure of DNA. (medscape.com)

Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
The transport of serum amino acids into cells is an active process that is facilitated by plasma membrane-localized amino acid transporters. (spandidos-publications.com)
The members of the L-type amino acid transporter (LAT) family are Na + -independent transporters that deliver neutral amino acids into cells ( 2 ). (spandidos-publications.com)
Free amino acid L-glutamine is known to be unstable in cell culture. (sigmaaldrich.com)

The researchers found that, based on empirical data from cell phones and GPS records, people are most inclined to travel to "attractive" locations they've visited before, and these movements are independent of the size of a region. (sciencedaily.com)
Following a spate of patient deaths in clinical trials testing modified T cells for the treatment of cancer, researchers work to reduce the treatment's toxicity without sacrificing efficacy. (the-scientist.com)

The presence of necrostatin-1, an inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK‑1), but not that of Z-VAD-fmk, attenuated the cytotoxic effects of GEF under AAS culture conditions. (spandidos-publications.com)
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. (sigmaaldrich.com)
Stericup Quick Release-GP Sterile Vacuum Filtration System, Stericup Quick Release-GP sterile vacuum filtration system combines a 500 mL Steritop bottle top filter unit, which uses a 0.22 µm pore size fast flow PES Express PLUS membrane, with a 500 mL receiver flask for sterilizing tissue culture media, protein & DNA. (sigmaaldrich.com)

The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture. (wikipedia.org)
He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
We use a tissue culture system called cerebral organoids that we have developed in 2013 and that can recapitulate brain development at a remarkable level of detail (Lancaster et al. (europa.eu)

In these terms, the characterizing feature is the cell's transcriptome: those genes that are transcribed into RNA to make working proteins, which differ from cell to cell. (pennmedicine.org)
Using deep RNA sequencing, they found over 12,000 expressed genes in the cells, including hundreds of different types of RNAs specific to the different cell types. (pennmedicine.org)
They also identified long noncoding RNAs involved in regulation of many other genes that correlated with cell type. (pennmedicine.org)

Since Warn first looked into the effects of e-cigarettes on respiratory health back in 2017, many studies have been conducted of the short-term health outcomes for first-time smokers who never used combustible tobacco products. (medscape.com)

The overall goal of this project is to use three-dimensional cell culture in order to elucidate how those processes are regulated and how their impairment can lead to neuro-developmental disorders. (europa.eu)
In this post, we will explore two examples of how perfusion systems can help improve the relevance of your 3D cell culture, based on two publications from Alvetex users in the field of developmental biology and cancer. (reprocell.com)

229th ENMC international workshop: Limb girdle muscular dystrophies - Nomenclature and reformed classification Naarden, the Netherlands, 17-19 March 2017. (medscape.com)

Have 4 connected wells, allowing for greater volume provision for highly metabolic monocultures, or active solute flow for paracrine or cell migration co-cultures. (reprocell.com)
NaCl Celecoxib 136.9 KCl 5.4 CaCl2 1.5 MgCl2 1.0 NaHCO3 23.8 ethylenediaminetetraacetic acidity 0.01) while reported previously [15 16 2 Cell isolation and tradition migration of rat cavernous soft muscle tissue cells was measured through the use of Matrigel with the next adjustments [13]. (sciencepop.org)

Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G. Serum-free cell culture: the serum-free media interactive online database. (springer.com)
BIO-MPM-1 Medium is a multi-purpose, serum-free medium for culture of a wide variety of anchorage-dependent cells. (bioind.com)
The sprouting cells had been subcultured in Dulbecco's revised eagle moderate (DMEM) including 920 mg/l D-valine 10 fetal bovine serum (FBS) 100 U/ml penicillin 100 g/ml streptomycin and 200 mM glutamine. (sciencepop.org)

This avenue of research is in line with the goals of the national BRAIN Initiative - including a cell census of neurons in the brain. (pennmedicine.org)
This procedure dissociates the neurons, and from this mixture, the team cultured the live neurons. (pennmedicine.org)
They found that each patient's neurons had a specific gene-expression profile that was consistent between cells. (pennmedicine.org)

If this is the kind of results you want to achieve with your in vitro cell models, then adding perfusion can be done with minimal disruption to your existing protocols. (reprocell.com)

Conclusions We created a two-stage cells tradition method employing a Matrigel-based sprouting tradition program to facilitate stromal cell sprouting and an adherent tradition program using D-valine to remove the contaminants of fibroblasts in to the soft muscle tissue cells. (sciencepop.org)

There are two reasons for culturing naturally adherent cells in suspension. (integra-biosciences.com)
After the addition of 2mM glutamine, BIO-MPM-1 Medium is ready to use for adherent cells. (bioind.com)
Z. Aberman, Adherent cells from placenta and use of same in disease treatment . (bioind.com)

Latest clinical trial report, "Bronchiolitis Global Clinical Trials Review, H1, 2017" added by Market Research HUB, provides an overview of Bronchiolitis clinical trials scenario. (openpr.com)

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing. (sigmaaldrich.com)

This study was supported by the National Institutes of Health Single Cell Analysis Program (U01 MH098953). (pennmedicine.org)
The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
This paper presents results from a six-year ethnographic study involving interviews, focus groups and other forms of engagement with the scientific research team and other stakeholders, including public groups, which sought to understand respondents' reactions to cultured red blood cells. (stir.ac.uk)
The aim of the present study was to establish a primary cell culture derived from polymorphous low grade adenocarcinoma (PLGA). (bvsalud.org)
In this study, we investigated the effects of TF extract on skin inflammation in an AD animal model and also investigated the mechanism responsible for the effects of TF extract in AD on the modulation of calcium channels and the activation of mast cells. (hindawi.com)
Because aging has been demonstrated to be directly related to the occurrence of cardiac disorders, in the present study, the ability of naringenin to prevent cardiac cell senescence was investigated. (hindawi.com)
The aim of this study was to analyze the influence of low-level laser therapy (=660 nm), with three different irradiation times, on the behavior of OD-21 cell line. (bvsalud.org)
2005). These fungi produce sever- lected in the present study by enrichment culture, using kraft al extracellular peroxidases, laccases, and additional oxidative lignin and kraft pulping lignin-enriched streams. (lu.se)

Zhang S. Atlantic, May 24, 2017. (cdc.gov)

Due to their simplicity, 2D techniques can't mimic the cells' in vivo environment, where they usually grow in three dimensional structures with complex cell-to-cell interactions. (integra-biosciences.com)
This will not only improve our understanding of the physiological basis of interactions in cyanobacterial co-cultures, but also will add value to the cyanobacteria collection and guide subsequent attempts to obtain pure (axenic) cultures. (dsmz.de)

Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). (sigmaaldrich.com)
Purpose Primary culture from the cavernous even muscle tissue cells from corpus cavernous cells may be difficult due to the fact of contaminants with fibroblasts. (sciencepop.org)
In addition, these fibroblasts were combined with lymphocytes or dendritic cells in the lamina propria mucosae. (karger.com)

Single cell analyses were performed on over 300 living cells. (pennmedicine.org)
Recent ultrastructural analyses have provided evidence for intracellular sorting of antenna structures in the epibiont cells, which occurs exclusively when they are in the symbiotic state. (dsmz.de)

While in infected A549 and Vero cells a pronounced cytopathic effect was observed COS7, 293T and Huh7.5 cells were most resistant. (wjgnet.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph. (wikipedia.org)
Both the embryonic and extra-embryonic cells start to talk to each other and become organised into a structure that looks like and behaves like an embryo," explains Professor Magdalena Zernicka-Goetz from the Department of Physiology, Development and Neuroscience, who led the research. (cam.ac.uk)

20162

Viability8

20182

Abstract1

Mutations4

20231

Bacteria4

Scientist3

Scaffold10

Cellular8

Differentiation5

Human16

Stem19

Mammalian cell2

Epithelial cells2

Endothelial cells2

Tumor8

Phenotype2

20222

Proliferation5

Genetic2

Grown8

20191

Diseases4

Successfully1

Methods3

Morphology2

Infectious2

Zika1

Organoid1

Laboratory3

Establishment1

Roles2

Uptake1

Influenza6

Mice3

Amino acid4

Researchers2

Protein3

Tissue culture9

Genes3

Respiratory1

Developmental2

Classification1

Migration2

Serum3

Neurons3

Vitro cell1

Stromal cell1

Adherent cells3

Clinical1

Murine1

Study8

Atlantic1

Interactions2

Fibroblasts3

Analyses2

Vero1

Embryonic cells2