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GelsChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, GasChromatography, Ion ExchangeChromatography, Thin LayerChromatography, LiquidChromatography, DEAE-CelluloseMolecular WeightMolecular Sequence DataElectrophoresis, Gel, Two-DimensionalChromatography, AgaroseElectrophoresis, Polyacrylamide GelAmino Acid SequenceKineticsMass SpectrometryGas Chromatography-Mass SpectrometryChromatography, Reverse-PhaseElectrophoresis, Gel, Pulsed-FieldHydrogen-Ion ConcentrationChromatography, PaperElectrophoresis, Agar GelSepharoseIsoelectric FocusingSubstrate SpecificityAmino AcidsEscherichia coliTandem Mass SpectrometryBase SequenceSpectrophotometry, UltravioletChemistryChemical PhenomenaCattleRabbitsChemical FractionationPeptide FragmentsSpectrometry, Mass, Electrospray IonizationTemperatureProtein BindingMethodsTrypsinLiverCarbohydratesElectrophoresisDrug StabilityRecombinant ProteinsCountercurrent DistributionHydrolysisChromatography, Micellar Electrokinetic CapillaryPeptidesMagnetic Resonance SpectroscopyCloning, MolecularCarbohydrate SequenceHydroxyapatitesBacterial ProteinsIndicators and ReagentsBinding SitesIsoelectric PointOligosaccharidesCell LineCentrifugation, Density GradientChemical PrecipitationHot TemperatureSpecies SpecificitySwineMacromolecular SubstancesRadioimmunoassayPolysaccharidesSolubilityDNAReproducibility of ResultsAmmonium SulfateImmunodiffusionUltracentrifugationIsoenzymesMicrochemistryReference StandardsProteinsCalibrationImmunoelectrophoresisLectinsBiological AssayCarrier ProteinsFatty AcidsSequence Homology, Amino AcidGlycoproteinsCarbohydrate ConformationRats, Inbred StrainsStereoisomerismCells, CulturedProteomicsBrain ChemistryAcetonitrilesPhysicochemical PhenomenaCell MembraneDetergentsGlycopeptidesSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Permeation ChromatographyHigh Performance LiPerformed using gel permSeparated by silica gel2018Size exclusion chro2017AnalPurificationSilica gel 60AnalyticalCharacterizationEtOAcSeparationsHPLCAffinityElectrophoresisStationary phasesColumnPROTEINSSeparationLiquidConsistTechniqueYearPlatesDevelopmentMassDataInstruments
Permeation Chromatography5
High Performance Li5
  • High-performance liquid chromatography with diode array detection system was used for identification and quantification of the monomers in the resin systems. (niom.no)
  • The researchers combined gel-permeation high-performance liquid chromatography with fluorescence detection to develop a sensitive technique that can detect both gliadins and glutenins in purified wheat starch. (healthnewsground.com)
  • Article: Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients by Gel-Permeation High-Performance Liquid Chromatography with Fluorescence Detection (GP-HPLC-FLD), Katharina Anne Scherf, Herbert Wieser, and Peter Koehler, Journal of Agricultural and Food Chemistry , doi: 10.1021/acs.jafc.6b02512, published online 16 September 2016. (healthnewsground.com)
  • Ultra-performance liquid chromatography (UPLC) has an advantage over conventional High-performance liquid chromatography (HPLC) as UPLC offers substantial resolution, speed, and sensitivity during analysis. (rjptonline.org)
  • This method uses online solid phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) for quantifying DEET and three of its metabolites, N,N-diethyl-3-hydroxymethylbenzamide (DHMB), N-ethyl-3-methylbenzamide (EMB), and 3-diethyl-carbamoyl benzoic acid (DCBA), in 100 µL of human urine. (cdc.gov)
Performed using gel perm1
  • Characterization of an oligomeric compound was performed using gel permeation liquid chromatography. (niom.no)
Separated by silica gel1
  • Combined with TLC bioautography assay and HPLC comparison, the four antioxidant components separated by silica gel chromatography and further purified by Sephadex LH-20 were determined to be caffeic acid, rosmarinic acid, luteolin and apigenin, respectively. (rsc.org)
20181
  • Preparation of a low bleeding polar stationary phase for hydrophilic interaction liquid chromatography, Talanta, 2018, 182, 500-504. (ecust.edu.cn)
Size exclusion chro1
  • Molar mass distribution measurements are performed by SEC-MALLS-RI, i.e. size-exclusion chromatography (SEC) with multi-angle laser light scattering (MALLS) / refractive index (RI) detection, in the solvent system N,N -dimethylacetamide (DMAc)/LiCl. (boku.ac.at)
20172
  • Poly(vinyl alcohol)-cationic cellulose copolymer encapsulated SiO2 stationary phase for hydrophilic interaction liquid chromatography, RSC Adv. 2017, 7, 21336-21341. (ecust.edu.cn)
  • A positively charged porous graphitic carbon stationary phase for hydrophilic interaction liquid chromatography, Talanta, 2017, 164, 159-163. (ecust.edu.cn)
Anal5
  • Online Gas-Free Electrodialytic KOH Eluent Generator for Ion Chromatography, Anal. (ecust.edu.cn)
  • Low-bleed silica-based stationary phase for hydrophilic interaction liquid chromatography, Anal. (ecust.edu.cn)
  • An integrated device of electrodialytic membrane suppressor and charge detector for ion chromatography, Anal. (ecust.edu.cn)
  • Poly(vinyl alcohol) Modified Porous Graphitic Carbon Stationary Phase for Hydrophilic Interaction Liquid Chromatography, Anal. (ecust.edu.cn)
  • 10. Yahui Peng, Yanjie Hou, Feifang Zhang, Guobin Shen, B. C. Yang* , A hyperbranched polyethylenimine functionalized stationary phase for hydrophilic interaction liquid chromatography, Anal Bioanal Chem. (ecust.edu.cn)
Purification8
  • dc.title: Evaluating Improvements and Challenges in Affinity Chromatography for AAV Purification dc.description.abstract: Purification process development is crucial to the Research and Development (R&D) sector in the biotechnology industry by designing and optimizing downstream unit operations capable of manufacturing safe treatments for clinical trials. (jhu.edu)
  • The isolation and purification involved a combination of methods including silica gel column chromatography, Sephadex LH-20, and semi-prep HPLC separations. (umn.edu)
  • Purification by silica gel display column chromatography (EtOAc/hexane) afforded the required bicyclic formyl derivatives 20a-d. (bio-biz-navi.com)
  • Nowadays, automating chromatography is helping to save time and effort in protein purification. (bioradiations.com)
  • This chromatogram and SDS-PAGE gel highlight the importance of protein purity assessment throughout your purification workflow. (bioradiations.com)
  • One of the most widely used methods for the isolation and purification of recombinant proteins is chromatography. (bioradiations.com)
  • Over the past three decades, chromatography, specifically liquid chromatography, has become the technology of choice in the purification of biotherapeutics, due in large part to its resolving power. (bioradiations.com)
  • Bio-Rad's NGC™ Chromatography Systems were designed with this is mind and provide a range of customizable chromatography systems that can increase purification capabilities and be adapted to meet growing throughput needs, obviating the need to buy a new instrument. (bioradiations.com)
Silica gel 602
  • The determination was carried on Silica Gel 60 GF254 HPTLC Plates using the mobile phase of chloroform:ethyl alcohol: Diethyl amine: water (12:7:1:0.4V/V). The absorbances of the spots were measured by densitometry at 254nm. (i-scholar.in)
  • Thin-layer chromatography (TLC) was performed on 0.2 Uramustine mm Merck silica gel 60 F254 silica plates (Merck Darmstadt Germany) and substances had been visualised under 245 nm ultraviolet irradiation and/or phosphomolybdic acidity. (cancerrealitycheck.com)
Analytical2
Characterization1
EtOAc2
  • LRMS-ES+ (relative intensity) 362 (MH+ ASC-J9 80 HRMS (to give a residue which was purified by flash chromatography on silica gel (gradient 20-50% EtOAc/hexanes) to yield diastereomeric Michael adducts 24a and 24b (orange foam 14.01 g 99 ~1.2:1 by 1H NMR) which were carried on to the next step without separation. (healthweeks.com)
  • Methyl 2-(3-(((to give a residue which was purified by flash chromatography on silica gel (30% EtOAc/hexanes) yielding = 5.7 Hz 0.5 5.06 (m 1 4.84 (d = 15.0 Hz 0.5 4.54 (m 2 3.69 (m 4 3.41 (dt = 7.1 12.6. (healthweeks.com)
Separations1
HPLC2
  • Priyanka G. Dhanalakshmi K. Nagarjun R. Switch from HPLC to UPLC: A novel achievement in liquid chromatography technique- A review. (rjptonline.org)
  • Thin level chromatography (TLC) and powerful liquid chromatographic (HPLC) behavior supplied further understanding into pigment polarity and therefore intramolecular hydrogen bonding. (researchatlanta.org)
Affinity3
  • One of the key polishing steps in the downstream manufacturing process of AAVs is affinity chromatography, where a ligand with specific binding affinity to an AAV serotype is coupled to a base matrix that separates AAVs from host cell contaminants produced in the cell culture process. (jhu.edu)
  • Column resin is the most common affinity chromatography matrix, but it possesses mass transfer limitations. (jhu.edu)
  • Various forms of chromatography, including affinity, ion exchange, and mixed mode, are now being utilized to ensure that the final product is as pure as can be without compromising yield. (bioradiations.com)
Electrophoresis2
  • A polyacrylamide gel electrophoresis revealed non-hydrolysed protein molecules with MW ≈ 20 kDa. (jfrm.ru)
  • Electrophoresis is often a companion technique to chromatography because it allows researchers an orthogonal method with which to visualize the protein species present in a sample. (bioradiations.com)
Stationary phases1
  • Modern two-dimensional chromatographic techniques are based on the results of the early developments of Paper chromatography and thin-layer chromatography (TLC) which involved liquid mobile phases and solid stationary phases. (wikipedia.org)
Column3
  • For instance, a C18 reversed-phase chromatography column may be followed by a phenyl column. (wikipedia.org)
  • Column chromatography was performed using Baker gel 60 (230e400 mesh) (Baker Deventer HOLLAND) using the indicated solvents. (cancerrealitycheck.com)
  • Upon observing the results of TLC, 1 g dried chloroform non-small-cell lung carcinoma fraction of the petroleum ether extract was subjected to column chromatography and loaded on a glass column (60 �� 3 cm) packed with silica gel G (40 g, 60�C120#, Spectrochem Pvt. (jakenzyme.com)
PROTEINS1
  • Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. (org.ua)
Separation4
  • Two-dimensional chromatography is a type of chromatographic technique in which the injected sample is separated by passing through two different separation stages. (wikipedia.org)
  • The earliest form of 2D-chromatography came in the form of a multi-step TLC separation in which a thin sheet of cellulose is used first with one solvent in one direction, then, after the paper has been dried, another solvent is run in a direction at right angles to the first. (wikipedia.org)
  • Gas chromatography-mass spectrometry (GC-MS) is a two-dimensional chromatography technique that combines the separation technique of gas chromatography with the identification technique of mass spectrometry. (wikipedia.org)
  • Preparation of decarboxylic-functionalized weak cation exchanger and application for simultaneous separation of alkali, alkaline earth and transition metals, Talanta, 2016, 153(1): 79-82. (ecust.edu.cn)
Liquid7
Consist1
Technique1
Year1
  • Every year, approximately one-third of the U.S. population uses DEET-containing insect repellent products with concentrations ranging from 10 to 100% in a variety of liquids, lotions, gels, sprays, sticks and impregnated materials and more than 30 million packages of DEET-containing products are sold annually. (cdc.gov)
Plates1
Development1
  • Development and Validation of Retention Models in Supercritical Fluid Chromatography for Impregnation Process Design. (tuhh.de)
Mass1
  • In addition, gas chromatography, infrared spectroscopy and combined gas chromatography/mass spectrometry were applied for identification purposes. (niom.no)
Data1
  • They were the largest group of students (95%) according to the 2015-2016 College Scorecard data · See all rates Graduation rate is for first-time, full-time undergraduate students who graduated within six years. (bearsofficialsstore.com)
Instruments1
  • Scientists can characterize drug targets and potential therapeutics with greater confidence when using stain-free gels and gel imaging instruments. (bioradiations.com)