• Using a population-based ever, these analyses are limited to those sites that vary approach that analyzes multiple loci around the chromo- between the compared genomes and are potentially mis- some, we demonstrate that neutral genetic variation in leading (13-15). (cdc.gov)
  • The 400 bp sequence of S. epidermidis ATCC 14990 was identified as the most specific sequence (Se400), having a 100% sequence similarity to S. epidermidis genomes but not with other bacteria. (ajmb.org)
  • Phylogenetic methods which do not rely on multiple sequence alignments are important tools in inferring trees directly from completely sequenced genomes. (biomedcentral.com)
  • Here, we extend the recently described Genome BLAST Distance Phylogeny (GBDP) strategy to compute phylogenetic trees from all completely sequenced plastid genomes currently available and from a selection of mitochondrial genomes representing the major eukaryotic lineages. (biomedcentral.com)
  • Nowadays, an increasing number of completely sequenced genomes are available and a growing field of phylogenetic research deals with the question of how to infer reliable phylogenies from this large amount of data to overcome the limitations of single-gene phylogenies. (biomedcentral.com)
  • A relatively obvious approach to phylogenetic analysis of whole genomes is to extract as many genes as possible from the genome sequences, create a multiple sequence alignment from each of the genes and to concatenate all alignments. (biomedcentral.com)
  • Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)
  • From the beginning, the project has also played a large part in driving the development of technology that aided the high-throughput sequencing of genomes from other model organisms such as mouse, worm and yeast. (ubc.ca)
  • The Human Genome Project has revealed that protein-coding genes represent less than 2% of the total genome sequence [ 7 ], and the remaining greater portion of human genomes are regarded as "junk DNAs", for they do not encode any protein. (oncotarget.com)
  • As the sequencing and gene annotation projects of entire genomes of many species are headed towards completion (see, e.g. [13] ), massive mapping efforts in biology are now focused on how the genes interact. (lu.se)
  • In the United Kingdom, Genomics England seeks to assess the feasibility, benefits, and risks of whole genome sequencing as part of the Newborn Genomes Programme , an analysis of 100,000 newborn genomes. (medscape.com)
  • Although France's initiative for genomic medicine, France Génomique 2025, does not envisage a neonatal genome sequencing screening program, a team in Dijon is studying several dozen genomes to determine the medical and financial benefits of such a program, explained Geneviève. (medscape.com)
  • As of August 10, 2000, draft sequence was available for 87% of the human genome (https://www.ncbi.nlm.nih.gov/genome/seq/), and at least 39 prokaryotic genomes, including those of more than a dozen human pathogens, had been completely sequenced https://www.tigr.org/tdb/mdb/mdbcomplete.html). (medscape.com)
  • Several independent high-throughput methods for differential gene expression (including SAGE and differential display) may enable function annotation of sequenced genomes [ 2 ] . (medscape.com)
  • A comparative taxonomic and phytogeographic analysis (S. L. Mosyakin 2002) also suggests the place of origin of the Salsola generic aggregate is somewhere in the Tethyan region of south-central Asia (probably northern coasts of the ancient Tethys, or adjacent inland lacustrine habitats). (efloras.org)
  • Nucleotide sequences provide robust, genes associated with antimicrobial drug resistance has sufficient variation to construct a robust phylogenetic tree portable, and comparable data for studying population for M. tuberculosis . (cdc.gov)
  • The phyloge- high-throughput gene sequencing of structural genes (19) netic structure is concordant with all the previously and host immune system protein targets (20) in M. tuber- described genotypic and phenotypic groupings of M. tuber- culosis strains and provides a unifying framework for both culosis isolates indicated low levels of sequence diversity. (cdc.gov)
  • Thus, sequence analysis can be used to assign function to genes and proteins by the study of the similarities between the compared sequences. (wikipedia.org)
  • since 1994 the potential advantages of such an approach have been demonstrated for a number of blowfly species using DNA sequences of both mitochondrial and nuclear genes. (exeter.ac.uk)
  • Euchromatin is a lightly packed form of chromatin (DNA, RNA, and protein) that is enriched in genes, and is often (but not always) under active transcription. (bcgsc.ca)
  • For the vast majority of genes, the sequence in these regions was indeed finished. (bcgsc.ca)
  • Characteristics of Clonal Hematopoiesis (CH) Mutations - a) We identified somatic mutations in known clonal hematopoiesis of indeterminate potential (CHIP) driver genes using peripheral blood mononuclear cells isolated from 14 astronauts who flew short space Shuttle missions lasting a median of 12 days between 1998-2001. (spaceref.com)
  • Using DNA sequencing followed by extensive bioinformatics analyses, researchers identified 34 mutations in 17 CH-driver genes. (spaceref.com)
  • The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)
  • DNA microarrays are simply platforms that consist of small solid supports onto which the sequences from thousands of different genes are attached at fixed locations. (medscape.com)
  • Difficulties with this approach may arise if orthologous genes cannot be identified with certainty or if the combined sequence length is still too small to give well-resolved trees. (biomedcentral.com)
  • 2021. Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages rarely carry antibiotic resistance genes. (ulaval.ca)
  • This DNA sequence contained in a genome contains the complete code that determines which genes and proteins will be present in human cells. (ubc.ca)
  • For instance, as a first step in understanding the genomic code we have learnt that the human genome is made of 3.2 billion nucleotide bases (of which there are four types: A, C, T, G). It is thought that over 30,000 genes are encoded by this sequence. (ubc.ca)
  • We have undertaken studies investigation aberrant DNA methylation resulting in changes in the expression of cancer-related genes as a possible epigenetic mechanism for Cd carcinogenesis. (cdc.gov)
  • In addition, IGS sequencing (e.g., sequencing of intergenic regions between the 16S and 23S rRNA genes) has been used to taxonomy studies, while partial sequencing of the gene gtfB , which encodes the enzyme glucosyltransferase B, has been used to investigate enzymatic activity and virulence 11-12 . (bvsalud.org)
  • In bioinformatics, sequence analysis is the process of subjecting a DNA, RNA or peptide sequence to any of a wide range of analytical methods to understand its features, function, structure, or evolution. (wikipedia.org)
  • This does not include further in-depth bioinformatics analysis. (eurekalert.org)
  • After my PhD, I moved into full-time bioinformatics, with a focus on protein sequence analysis. (edu.au)
  • As a postdoc in Dublin, I developed a bioinformatics (sequence analysis) method for the rational design of biologically active short peptides. (edu.au)
  • 2001-2005: Postdoctoral Research Fellow in Bioinformatics, Royal College of Surgeons in Ireland, Dublin, Ireland. (edu.au)
  • Bioinformatics 2001,17(8):754-755.PubMedCrossRef 24. (mirnainhibitor.com)
  • DNA isolation, genotyping of simple tandem repeat polymorphisms, PCR amplification and sequencing were performed using standard techniques 2 and all simple tandem repeat polymorphisms were amplified in the presence of 10% glycerol and 5% DMSO after labelling one oligonucleotide primer with 32 P and analysed on a 6% polyacrylamide gel. (bmj.com)
  • Duties include the co-ordination of services provided by the laboratory and evaluation of methods for extraction and purification, PCR amplification, sequencing, and analysis of all DNA sample types. (lakeheadu.ca)
  • Phylogenetic analysis with DNA amplification technologies. (who.int)
  • The developed PCR-DGGE analysis of the "cultivable" community showed good potential in evaluating microbial diversity of a dairy environment: it usefully highlighted the bias introduced by selective amplification when compared to the analysis of the total community from NWC and allowed suitability of media and growth conditions to be evaluated. (who.int)
  • I am now involved in multiple de novo whole genome sequencing and assembly projects, using short read (Illumina), long read (PacBio & Nanopore) and linked read (10x Chromium) sequencing. (edu.au)
  • We performed whole-genome (WG) de novo sequencing and complete assembly of S. fibuligera KJJ81 and KPH12, two isolates from wheat-based Nuruk in Korea. (biomedcentral.com)
  • In the future, DNA samples could be taken from babies for whole genome sequencing to look for diseases that are likely to crop up later in life. (medscape.com)
  • Over this time, developments in obtaining nucleotide sequence improved greatly, leading to the publication of the first complete genome of a bacteriophage in 1977. (wikipedia.org)
  • CLONING, EXPRESSION, AND NUCLEOTIDE-SEQUENCE OF THE LACTOBACILLUS-HELVETICUS-481 GENE ENCODING THE BACTERIOCIN HELVETICIN-J. JOURNAL OF BACTERIOLOGY, 172(11), 6339–6347. (ncsu.edu)
  • Then the results were processed by the BioEdit software and the nucleotide sequence was analyzed using the Basic Local Alignment Search Tool (BLAST). (hindawi.com)
  • The HGP was an initiative started in the early 1990's that has involved the efforts of hundreds of scientists to generate high-quality reference sequence for the 3 billion base pairs of nucleotide sequence that make up the human genome. (ubc.ca)
  • random amplified polymorphic DNA analysis and mitochondrial DNA sequences. (exeter.ac.uk)
  • They performed phylogenetic analysis of 37 different mitochondrial DNA (mtDNA) control sequences from domesticated horses and calculated a mean divergence of 2.6%, suggesting that these sequences stem from ancient origins. (the-scientist.com)
  • the comparative analysis of mitochondrial DNA sequence strongly supports the cyclostome monophyly. (palass.org)
  • Doubly uniparental inheritance (DUI) provides an intriguing system for addressing aspects of molecular evolution and intermolecular recombination of mitochondrial DNA. (doe.gov)
  • both studies, comparable sequence data were obtained on a limited number of highly selected isolates. (cdc.gov)
  • U.K. clinical isolates, silent single nucleotide polymor- variation in insertion sequences (4) and repetitive genom- phisms (sSNPs) resolve an unambiguous phylogeny and ic elements (5,6), have elucidated clusters of identical and provide a unifying framework for epidemiologic, popula- closely related strain families (7-9). (cdc.gov)
  • The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex. (who.int)
  • To determine the detection limit and analytical specificity, pure genomic DNA of 18 bacteria include 12 standard strains (one S. epidermidis and 11 non- S. epidermidis ) and six clinical isolates (five S. epidermidis and one non- S. epidermidis ) were used. (ajmb.org)
  • The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. (bvsalud.org)
  • Next, the isolates were differentiated by sequencing a specific region of the gene encoding the enzyme glucosyltransferase B ( gtfB ). (bvsalud.org)
  • By sequencing the 16S-23S intergenic region, it was found that all of the isolates belonged to the species S. mutans . (bvsalud.org)
  • Based on the genetic similarity of the isolates and pattern of amino acid variations identified by partial sequencing of the gtfB gene, base-pair changes were identified and correlated with different virulence patterns among the isolates. (bvsalud.org)
  • In general, the matches in the database are ordered to show the most closely related sequences first, followed by sequences with diminishing similarity. (wikipedia.org)
  • DNA sequencing and sequence similarity analysis identified one of the aberrantlyl methylated DNA fragments as the p16 tumor suppressor gene. (cdc.gov)
  • This cDNA can then be fluorescently labeled and hybridized to the target gene sequences on the microarray. (medscape.com)
  • Since the development of methods of high-throughput production of gene and protein sequences, the rate of addition of new sequences to the databases increased very rapidly. (wikipedia.org)
  • During my second postdoc, I coined the term "Short Linear Motif" (SLiM) for a specific type of protein interaction motif and was instrumental in developing SLiMDisc and SLiMFinder, two of the first algorithms for successfully predicting SLiMs from protein sequences. (edu.au)
  • Complete genomic sequences of microbial pathogens and hosts offer sophisticated new strategies for studying host-pathogen interactions. (medscape.com)
  • The completely sequenced S. fibuligera genome with high-quality annotation and RNA-Seq analysis establishes an important foundation for functional inference of S. fibuligera in the degradation of fermentation mash. (biomedcentral.com)
  • This hypothesis was tested based on phylogenetic analysis of the rDNA ITS region, morphological characters of basidiomes and pure cultures, growth rates and optimum growth temperature experiments, mycelial confrontation tests, enzyme activity tests and volatile organic compound (VOC) production. (springer.com)
  • Its flagellin and 16S rRNA central Europe, we screened questing ticks for 16S rDNA sequences resemble those of the relapsing fever spirochetes similar to that of Asian and American relapsing fever-like more closely than those of any of the known Lyme disease spirochetes. (cdc.gov)
  • The 16S rDNA V3 region was amplified using DNA from NWC as well as DNA from bulks as templates and the amplicons were separated by DGGE. (who.int)
  • The microbial entities occurring in NWCs were identified by partial 16S rDNA sequencing of DGGE bands: four lactic acid bacteria (LAB) closest relative of Streptococcus thermophilus, Lactococcus lactis, Lactobacillus delbrueckii and Lactobacillus crispatus were revealed by the analysis of DNA directly extracted from NWC while two other LAB, Lactobacillus fermentum and Enterococcus faecalis, were identified by analyzing DNA from the cultivable community. (who.int)
  • The rpoB gene target sequence was amplified using mutagenesis forward and reverse primers followed by HindIII enzyme digestion. (who.int)
  • Samples were extracted using the GeneJET Genomic DNA Purification Kit, then amplified by PCR using primers 63F and 1387r. (adoc.pub)
  • Foi usado um total de 78 primers RAPD, sendo que 8 apresentaram 54% de fragmentos de DNA polimórficos. (scielo.br)
  • Direct DNA sequencing was performed using the same PCR primers. (who.int)
  • If the sequence result was of low quality, the amplicon was cloned into a plasmid vector using a TOPO TA cloning kit (Invitrogen, Carlsbad, California) and then sequenced using the primers provided with the kit. (who.int)
  • 2001) recently discussed phylogenetic relationships inferred from parsimony analysis of nucleotide sequences of the internal transcribed spacer regions (ITS) of the 18S-26S nuclear ribosomal DNA of 34 species of Salsola and related genera ( Halothamnus Jaubert & Spach, Climacoptera Botschantzev, Girgensohnia Bunge, Halocharis Moquin-Tandon, and Haloxylon Bunge) and four species from representative outgroups (tribes Camphorosmeae and Atripliceae). (efloras.org)
  • These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. (nih.gov)
  • These two phage clones specifically hybridized to L. helveticus 481 total genomic DNA but not to DNA from strains that did not produce helveticin J or strains producing unrelated bacteriocins. (ncsu.edu)
  • WHO Collaborating Centre for Orthopoxvirus Diagnosis and Repository for Variola Virus Strains and DNA, State Research Centre of Virology and Biotechnology "VECTOR", Koltsovo, Novosibirsk Region, Russian Federation. (who.int)
  • The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)
  • The new methods of real-time PCR and DNA microarrays offer the potential for widespread application and utility in the future. (intechopen.com)
  • The 2 most common microarray technologies in use are the oligonucleotide microarrays and the robotically spotted complementary DNA (cDNA) microarrays. (medscape.com)
  • In what follows we limit ourselves to the analysis of gene expression measurements obtained using microarrays with small spots of DNA fixed to glass slides. (lu.se)
  • DNA microarrays exploit primary sequence data to measure transcript levels and detect sequence polymorphisms, for every gene, simultaneously. (medscape.com)
  • Cite this: Using DNA Microarrays to Study Host-Microbe Interactions - Medscape - Sep 01, 2000. (medscape.com)
  • Because of the complexity of the data sets generated by microarray experiments, the use of data-analysis software is essential. (medscape.com)
  • The design and construction of a DNA microarray for any given microbial genome are straightforward. (medscape.com)
  • DNA microarray hybridization analysis stands out for its simplicity, comprehensiveness, data consistency, and high throughput. (medscape.com)
  • The molecular characterization was performed by carrying out sequencing of DNA ITS region. (hindawi.com)
  • The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. (scielo.br)
  • The structure reveals that Cdc6 contributes to origin DNA recognition via its winged helix domain (WHD) and its initiator-specific motif. (nature.com)
  • Although the agenda of these authors was not taxonomic, their phylogenetic analysis of DNA sequence data reveals that "the tropical American [ Magnolia ] section Talauma branched first," i.e., is sister to the rest of the genus. (mobot.org)
  • Deep oncopanel sequencing reveals within block position-dependent quality degradation in FFPE processed samples. (musc.edu)
  • Database analysis reveals that all infected with Type I, while the age groups were affected. (cdc.gov)
  • Two important algorithms for aligning pairs of sequences are the Needleman-Wunsch algorithm and the Smith-Waterman algorithm. (wikipedia.org)
  • The HGP started at a meeting of scientists during which the value of knowing the genome sequence of an organism was recognized. (ubc.ca)
  • Two large groups of scientists published the first analyses of this human genome sequence in the February 2001 issues of the journals Nature [1] and Science [2]. (ubc.ca)
  • The race to publish human genome sequence information was fuelled by competition between research from the publicly funded HGP and the privately owned company, Celera Genomics. (ubc.ca)
  • This first working draft of the human genome sequence was hailed with much excitement and fanfare as the "completion of the human genome" in the media. (ubc.ca)
  • With genome sequence in-hand scientists are now more effectively able to study gene function and explore new areas of research such as how human variation contributes to different diseases worldwide. (ubc.ca)
  • These spirochetes differ genet- disease spirochetes generally fail to amplify DNA from ically from their American and Asian analogs while being these organisms. (cdc.gov)
  • Since the very first sequences of the insulin protein were characterized by Fred Sanger in 1951, biologists have been trying to use this knowledge to understand the function of molecules. (wikipedia.org)
  • The method used in this study, which is called the "Sanger method" or Sanger sequencing, was a milestone in sequencing long strand molecules such as DNA. (wikipedia.org)
  • However, traditional Sanger sequencing-based DNA barcoding protocols can be prohibitively expensive and laborious when large community samples have to be processed. (springer.com)
  • The results of MSRF were confirmed by southern hybridization analysis using the aberrantly methylated DNA fragments as the probes. (cdc.gov)
  • Such a collection of sequences does not, by itself, increase the scientist's understanding of the biology of organisms. (wikipedia.org)
  • Revealing the evolution and genetic diversity of sequences and organisms Identification of molecular structure from sequence alone In chemistry, sequence analysis comprises techniques used to determine the sequence of a polymer formed of several monomers (see Sequence analysis of synthetic polymers). (wikipedia.org)
  • The systematic study of the complete DNA sequences (GENOME) of organisms. (bvsalud.org)
  • Although many persons may be bitten by ticks infected by whether such organisms are present in Europe, we relapsing fever-like spirochetes, health implications remain screened questing ticks for DNA similar to that of the unknown. (cdc.gov)
  • By comparing sequences from these different model organisms, scientists gain a better understanding of the important pieces of code in genomic DNA sequence since conservation of sequences between two organisms that diverged phylogenetically millions of years ago, like humans and worms, implies that the conserved sequence is important for function. (ubc.ca)
  • The DNA is that of dr Marjolein Kriek, a clinical geneticist at LUMC. (eurekalert.org)
  • If anyone could properly consider the ramifications of knowing his or her sequence, it is a clinical geneticist," says professor Gert-Jan B van Ommen, leader of the LUMC team and director of the 'Center for Medical Systems Biology' (CMSB), a center of the Netherlands Genomics Initiative. (eurekalert.org)
  • Molecular analysis using targeted next generation DNA sequencing and clinical spectrum of Mexican patients with isovaleric acidemia. (musc.edu)
  • CLINICAL RELEVANCE STATEMENT: Among multiple sequences, the ADC radiomics model has a potential for generalizability and applicability in clinical practice. (bvsalud.org)
  • Hernandez-Vargas H, Lambert MP, Le Calvez-Kelm F, Gouysse G, McKay-Chopin S, Tavtigian SV, Scoazec JY, Herceg Z. (2010) Hepatocellular carcinoma displays distinct DNA methylation signatures with potential as clinical predictors. (who.int)
  • Cruz Garcia Rodriguez Address Unidad de Inmunologia, Hospital La Paz, Paseo de Address la Castellana 261, 28046 Madrid, Spain Telephone +34-91-7277095 Fax +34-91-7277095 Email [email protected] Method SSCP, Direct sequencing Frequency Whenever required Samples 2 samples per year Prices 300 EUR/patient 112/carrier Comment Patient samples are only accepted for analysis after Comment prior consultation and submission of clinical and Comment laboratory data. (lu.se)
  • SSCP Frequency Weekly Samples 12 patients /year Prices 482 EUR/patient, 180 EUR/carrier Comment Patient samples are only accepted for analysis after Comment prior consultation and submission of clinical and Comment laboratory data. (lu.se)
  • High-throughput sequencing (HTS), particularly DNA metabarcoding, enables community-wide analyses of diversity and interactions at unprecedented scales and at a fraction of the cost that was previously possible. (springer.com)
  • The emergence of high-throughput sequencing technologies (HTS) has been a significant step forward in recent years, greatly reducing both the cost and the labor required for biodiversity studies (Bohmann et al. (springer.com)
  • High-throughput methods for assessment of function are clearly required if this wealth of primary sequence information is to be used. (medscape.com)
  • In the last two decades, DNA barcoding, the sequencing of short species-specific amplicons, has considerably simplified community analyses (Hebert et al. (springer.com)
  • 2003 ). DNA barcodes can provide information on genetic variation within and between species, rapidly assign taxonomic status across divergent lineages (Hebert and Gregory 2005 ), and identify the prey composition of predators' gut contents (Agustí et al. (springer.com)
  • Wells, J.D., R. Wall & J.R. Stevens (2007) Phylogenetic analysis of forensically important Lucilia flies based on cytochrome oxidase I sequence: a cautionary tale for forensic species determination. (exeter.ac.uk)
  • Recent phylogenetic analyses based on multiple genetic markers indicated that F. fomentarius possibly contained cryptic species (Pristas et al. (springer.com)
  • Table 1 Fomes sequences included in the phylogenetic analysis with information on the species identification, the newly sequenced voucher, the GenBank Accession number, and available information on geographic provenance as well as on host plant and isolation source. (springer.com)
  • We are using the latest generation of sequencing, assembly and scaffolding technologies (10x Chromium "linked reads", PacBio, Oxford Nanopore, HiC) to assemble multiple species including venomous snakes, the cane toad, marsupials, dog breeds, and Australian flora. (edu.au)
  • The analysis by telomeric fingerprinting showed polymorphism between both species and the chromosome minimal numbers estimated were three. (scielo.br)
  • Methodologies used include sequence alignment, searches against biological databases, and others. (wikipedia.org)
  • Nowadays, there are many tools and techniques that provide the sequence comparisons (sequence alignment) and analyze the alignment product to understand its biology. (wikipedia.org)
  • Relationships between these sequences are usually discovered by aligning them together and assigning this alignment a score. (wikipedia.org)
  • There are two main types of sequence alignment. (wikipedia.org)
  • Pair-wise sequence alignment only compares two sequences at a time and multiple sequence alignment compares many sequences. (wikipedia.org)
  • A common use for pairwise sequence alignment is to take a sequence of interest and compare it to all known sequences in a database to identify homologous sequences. (wikipedia.org)
  • This result was followed by sequencing and alignment of data on Basic Local Alignment Search Tool (BLAST). (adoc.pub)
  • Analysis of covariation in an SH3 domain sequence alignment: applications in tertiary contact prediction and the design of compensating hydrophobic core substitutions. (ucm.es)
  • Nucleotide sequences were initially checked using the Basic Local Alignment Search Tool hosted by the National Center for Biotechnology Information ( http://blast.ncbi.nlm.nih.gov/Blast.cgim ) for comparison with other known nucleotide sequences. (who.int)
  • The DNA sequence and comparative analysis of human chromosome 20. (nih.gov)
  • Molecular phylogenetic analysis, tests on growth characteristics, enzyme assays, and comparative analysis of volatile compounds, were carried out for this purpose. (springer.com)
  • A team of researchers collected blood samples from National Aeronautics and Space Administration (NASA) astronauts who flew space shuttle missions between 1998 and 2001. (spaceref.com)
  • The study subjects were astronauts who flew relatively short (median 12 days) space shuttle missions between 1998 and 2001. (spaceref.com)
  • Stevens, J. & Wall, R. (1995) The use of random amplified polymorphic DNA (RAPD) analysis for studies of genetic variation in populations of the blowfly Lucilia sericata in southern England. (exeter.ac.uk)
  • A caracterização molecular foi baseada em marcadores RAPD, sequenciamento da região do espaçador interno transcrito do DNA ribossomal e "fingerprinting" telomérico. (scielo.br)
  • RAPD (Random Amplified Polymorphic DNA) markers have been most widely used for this purpose 8-10 . (bvsalud.org)
  • Although extensive genomic sequencing was performed in populations. (cdc.gov)
  • Neighbor-Joining, Maximum Parsimony and Maximum Likelihood analysis revealed Jakun and Semelai haplotypes are the earliest to split from ingroups and showed that Jakun may be an ancestor for the Malay populations in the Malay Peninsular, which also support the anthropological findings. (scialert.net)
  • Yet we have also discovered that over 50% of the human genome is repetitive sequence that does not code for any proteins and the function of this large portion of "junk" DNA is still puzzling scientists. (ubc.ca)
  • You've probably already heard - and have been hearing for the last 15 years - that the human genome has been sequenced. (bcgsc.ca)
  • For modeling and analysis - such as in cancer research, for example, which is what we do here - by far the most important parts of the human genome assembly are the parts that code for protein ( transcribed regions and their ORFs ), along with their adjacent regulatory sequences . (bcgsc.ca)
  • DNA Analysis of Human Skeletal Remains from the 1845 Franklin Expedition" Journal of Archaeological Science: Reports 2017, 16:409-419. (lakeheadu.ca)
  • Repeats and correlations in human DNA sequences. (ucm.es)
  • 4. Mesri EA, Feitelson MA, Munger K. Human viral oncogenesis: a cancer hallmarks analysis. (ac.ir)
  • By reading the sequence of the human genome, scientists hope to gain an understanding of the underlying code that determines how a complex biological system, such as a human cell, acts and reacts. (ubc.ca)
  • Officially, funding for the project began in the 1990 with the goal of sequencing the human genome by 2005. (ubc.ca)
  • For scientists, the high-quality reference sequence publicly released in April 2003 represents the first real step to having "finished" human sequence on hand (this draft represents sequence information that is considered to be 99% complete) [3]. (ubc.ca)
  • Conclusions: The DL-based software for automated T2 map analysis yields non-inferior measurements at the per-segment level and good performance for detecting myocardial segments with elevated T2 values compared with manual analysis. (bvsalud.org)
  • Conclusions: The partial sequencing of the gtfB gene can be a useful tool for elucidating the colonization patterns of S. mutans . (bvsalud.org)
  • They sequenced mtDNA segments in 191 horses from 10 distinct breeds and compared modern horses with ancient DNA from archaeological sites. (the-scientist.com)
  • You are hearing Gamma Crystallin Music , a representation of the amino acid sequence of a lens protein. (whozoo.org)
  • Note: all of the sequences from the links immediately above are simple linear readouts of the amino acid sequences of the proteins indicated. (whozoo.org)
  • Cloning and expression of the gene for lactacin F, a Lactobacillus acidophilus bacteriocin, using an amino acid sequence-derived DNA probe. (ncsu.edu)
  • Covariance analysis of RNA recognition motifs identifies functionally linked amino acids. (ucm.es)
  • Molecular phylogenies of many taxonomic groups are based on analyses of single loci. (biomedcentral.com)
  • Sampel diekstraksi menggunakan GeneJET Genomic DNA Purification Kit, kemudian diamplifikasi dengan PCR menggunakan primer 63f dan 1387r. (adoc.pub)
  • Positive PCR results are indicated by the presence of DNA fragments in the electrophoresis gel in the area about 2500 base pairs. (adoc.pub)
  • Hasil PCR positif ditunjukkan dengan adanya fragmen DNA pada gel elektroforesis pada daerah sekitar 2500 pasang basa. (adoc.pub)
  • Included is construction of complete genetic, physical, and transcript maps, and the analysis of this structural genomic information on a global scale such as in GENOME WIDE ASSOCIATION STUDIES. (bvsalud.org)
  • In this study, in a retrospective blinded analysis of stored twin samples, all 16 samples have been determined correctly, with four trisomy 21 positive and 12 trisomy negative samples. (mdpi.com)
  • A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity. (doe.gov)
  • Methods: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. (bvsalud.org)
  • Total microbial community was assessed without cultivation by analyzing DNA directly extracted from the original samples of NWC. (who.int)
  • A. Brooimans Address Wilhelmina Children's Hospital Dept. of Immunology Address Lundlaan 6, 3584 EA Utrecht, The Netherlands Telephone +31-302504354 Fax +31-302505311 Email [email protected] Method Direct sequencing Turnaround 1 month Samples 8 // Disease Wiskott-Aldrich syndrome and X-linked thrombocytopenia Accession I0009 Date 17-Dec-1999 (Rel. (lu.se)
  • Tick samples (3-5 ticks) were frozen and mashed by liquid nitrogen and then deoxyribonucleic acid (DNA) was extracted using the G-spin genomic DNA extraction kit (iNrRON Biotechnology Inc., Republic of Korea). (who.int)
  • Chromosome 9 spans about 150 million base pairs of nucleic acids (the building blocks of DNA ) and represents between 4.0 and 4.5% of the total DNA in cells . (wikipedia.org)
  • 22 billion base pairs (the 'letters' of the DNA language) were read. (eurekalert.org)
  • Chromosome 18 spans about 78 million DNA building blocks (base pairs) and represents approximately 2.5 percent of the total DNA in cells. (medlineplus.gov)
  • We then address the development of theoretical frameworks for community-level studies, and finally highlight critical gaps and future directions for DNA analysis of spider communities. (springer.com)
  • However, this first draft was not considered to be complete by scientists because of significant gaps in the sequences (this draft was 90% complete). (ubc.ca)
  • Genomic DNA isolated from BALB/c-3T3 cells morphologically transformed with Cd was restriction digested with Mse1 (methylation non-sensitive) alone or with MSe1 and BstU1 (methylation sensitive). (cdc.gov)
  • The resulting DNA was analyzed for differential methylation using a PCR-based technique - Methylation Sensitive Restriction Fingerprinting (MSRF). (cdc.gov)
  • To date, information on plasmid analysis in Pandoraea spp. (frontiersin.org)
  • The information on plasmid sequences in Pandoraea spp. (frontiersin.org)
  • is useful as the sequences did not match any known plasmid sequence deposited in public databases. (frontiersin.org)
  • In the budding yeast Saccharomyces cerevisiae , there are over 400 origins of DNA replication located on 16 chromosomes and they can function as autonomously replicating sequences (ARSs) when inserted into a plasmid 19 . (nature.com)
  • Successful transfection of hosts bearing pTR2030 indicated that the plasmid did not retard passage of naked phage DNA across the membrane. (ncsu.edu)
  • Lactobacillus helveticus 481 produces a 37-kDa bacteriocin called helveticin J. Libraries of chromosomal DNA from L. helveticus were prepared in lambda gt11 and probed for phage-producing fusion proteins that could react with polyclonal helveticin J antibody. (ncsu.edu)
  • HJ1 and HJ4 lysogens produced beta-galactosidase fusion proteins that shared similar epitopes with each other and helveticin J. The intact helveticin J gene (hlv) was isolated by screening a library of L. helveticus chromosomal DNA in lambda EMBL3 with the insert DNA from phage HJ4 as a probe. (ncsu.edu)
  • Knowledge of a genome unlocks the secrets of what DNA is making which proteins. (ubc.ca)
  • In social sciences and in sociology in particular, sequence methods are increasingly used to study life-course and career trajectories, time use, patterns of organizational and national development, conversation and interaction structure, and the problem of work/family synchrony. (wikipedia.org)
  • Wells, J.D. & J.R. Stevens (2008) Application of DNA-Based Methods in Forensic Entomology. (exeter.ac.uk)
  • de Queiroz, K. and S. Poe (2001) Philosophy and phylogenetic inference: a comparison of likelihood and parsimony methods in the context of Karl Popper's writings on corroboration. (berkeley.edu)
  • Non-invasive prenatal testing (NIPT) by random massively parallel sequencing of maternal plasma DNA for multiple pregnancies is a promising new option for prenatal care since conventional non-invasive screening for fetal trisomies 21, 18 and 13 has limitations and invasive diagnostic methods bear a higher risk for procedure related fetal losses in the case of multiple gestations compared to singletons. (mdpi.com)
  • Furthermore, the use of concatenated multiple sequence alignments discards information that can be utilised by other methods of phylogenetic inference. (biomedcentral.com)
  • Methods: Eighty-three subjects were retrospectively enrolled from two centers (42 healthy subjects and 41 patients with myocarditis) to validate a commercial DL-based software that was trained to segment the left ventricular myocardium and measure T2 values on T2 mapping sequences. (bvsalud.org)
  • The ion occupancies derived from the MRD data are substantially higher than previously reported, suggesting that groove-bound ions significantly influence the energetics and structural polymorphism of DNA in vivo. (lu.se)
  • In marketing, sequence analysis is often used in analytical customer relationship management applications, such as NPTB models (Next Product to Buy). (wikipedia.org)
  • Keyword: PCR, DNA sequencing, 16S rRNA gene Abstrak Diare dapat disebabkan oleh infeksi bakteri. (adoc.pub)
  • Proses PCR menggunakan sepasang primer, yang merupakan oligonukleotida yang berperan sebagai amplifikasi molekul DNA, kemudian dielektroforesis dan diidentifikasi menggunakan suatu gen penanda yaitu gen 16S rRNA (Aris 2013). (adoc.pub)
  • In this paper, we describe the genetic analysis of four IMPase homologues of M. tuberculosis. (mirnainhibitor.com)