• Mouse IL-17C cDNA encodes a 194 amino acid (aa) residues protein with a putative 14 aa signal peptide (5). (rndsystems.com)
  • The hOAT3 cDNA consisted of 2179 base pairs that encoded a 543-amino-acid residue protein with 12 putative transmembrane domains. (aspetjournals.org)
  • Rapid and comprehensive analysis of putative proteinous allergens ( allergenome ) by applying such a proteomic strategy … With allergenomics, we can not only detect and assign the putative allergens (proteins specifically interacting with IgE antibodies in a patient's blood) in a short time, but also analyze the quantitative and qualitative change of the antigens, depending on the surroundings and environmental conditions of an allergenic causative. (genomicglossaries.com)
  • A third of the transcriptome showed similarity with previously characterized immune-related genes that included both the known and putative immune genes. (biomedcentral.com)
  • Of the four putative novel defence proteins (DFPs) annotated by PSI-BLAST three showed similarity to extracellular matrix proteins from vertebrates implicated in innate immunity, while the fourth was similar to, yet distinct from, the anti-microbial protein cecropin. (biomedcentral.com)
  • The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)
  • Genes encoding putative orthologues of NnrS are sometimes but not always found in bacteria encoding nitrite and/or nitric oxide reductase. (microbiologyresearch.org)
  • In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
  • We have identified four members of this subfamily in Arabidopsis thaliana, one of them with associated expressed sequence tags (ESTs). (usu.edu)
  • We identified 719 unique ESTs from a total of 1412 sequences so generated. (biomedcentral.com)
  • With reference to insect immunity, the ESTs have been obtained from baculovirus-infected B. mori cultured cells and pupae, but no large scale information on bacteria-induced immune genes is as yet available. (biomedcentral.com)
  • Automatic comparisons of data from expressed sequence tags (ESTs) with genes (for example using the program Acembly, for which the results are available from the NCBI AceView server [ 2 ]) shows that all the human protein prenyltransferase genes have multiple alternative splice variants. (biomedcentral.com)
  • ud \ud Methods and Results: We aligned expressed sequence tags (ESTs) from Ceratodon purpureus to the Physcomitrella patens genome sequence, and designed primers that are homologous to … Here we examine the genetic and genome size diversity in Mediterranean populations of the moss Ceratodon purpureus s.l. (oosteinde.info)
  • Anticipated orthologous ORFs in the regions above were defined as BLAT (Kent 2002) or SSearch (Pearson and Lipman 1988) sequence matches. (ubc.ca)
  • Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)
  • An expressed sequence tag (EST) library was constructed from mRNA obtained from fat bodies of A. mylitta larvae that had been challenged by infection with Escherichia coli cells. (biomedcentral.com)
  • This lack of increase contrasts with previous reports as does the mRNA length: this is 50% larger as confirmed by sequencing the 3' end. (embl.de)
  • After mRNA processing, most transcripts are exported to the cytoplasm for translation into protein. (berkeley.edu)
  • Each mRNA transcript can serve as template for repeated translation into protein by ribosomes. (berkeley.edu)
  • The number of protein products produced by any single mRNA can vary widely. (berkeley.edu)
  • In the present study, we report the characterization of the full coding sequence of rainbow trout PGMRC1 and mPR beta cDNAs, their tissue distribution, their ovarian expression profiles during oogenesis, their hormonal regulation in the full grown ovary and the in situ localization of PGMRC1 mRNA in the ovary. (biomedcentral.com)
  • In addition, the mRNA abundance of both genes is not increased after in vitro hormonal stimulation of full grown follicles by maturation inducing hormones. (biomedcentral.com)
  • The presence of a stop codon in exon 13 generates two families of Sec31B gene products (each displaying additional patterns of mRNA splicing): a group of full-length proteins (hereafter referred to as Sec31B-F) and also a group of truncated proteins (hereafter referred to as Sec31B-T), distinguished by their utilization of exon 13. (biologists.com)
  • Recombinant histidine-tagged WU-VP1 protein was generated by expressing WU-VP1 (GenBank accession no. (cdc.gov)
  • Purification of a histidine-tagged NnrS demonstrated that NnrS is a haem- and copper-containing membrane protein. (microbiologyresearch.org)
  • In the present study we constructed and analysed the immune transcriptome of the lepidopteran Antheraea mylitta , an economically important Indian tasar silkmoth with a view to unravel the potential immune-related genes and pathways. (biomedcentral.com)
  • We also validated the upregulation of a selected subset of genes from the immune transcriptome by semi-quantitative RT-PCR. (biomedcentral.com)
  • Since many of today's expression vectors encode Shine-Dalgarno sequences it is easy to overlook problems with translation initiation. (neb.com)
  • The genomic organization of the human genes that encode protein prenyltransferases is shown in Figure 1 . (biomedcentral.com)
  • One-third of the 471 genes predicted to encode transcription factors are differentially expressed during sexual development of S. commune . (nature.com)
  • Actin is one of the most abundant proteins in yeast, mammalian and other eukaryotic cells. (elifesciences.org)
  • This is particularly true when expressing eukaryotic genes in bacterial hosts. (neb.com)
  • Three different protein prenyltransferases (farnesyltransferase and geranylgeranyltransferases I and II) catalyze the attachment of prenyl lipid anchors 15 or 20 carbons long to the carboxyl termini of a variety of eukaryotic proteins. (biomedcentral.com)
  • The chromosomal locations and number of exons from protein prenyltransferase genes in the major eukaryotic model organisms are shown in Table 2 . (biomedcentral.com)
  • A cDNA sequence encoding the sequence of GDF7 was constructred and used to recombinantly synthesize the protein. (enquirebio.com)
  • Apelin gene encodes a pre-proprotein of 77 amino acids, with a signal peptide in the N-terminal region. (phoenixbiotech.net)
  • Mutations in the SLC6A19 gene, which encodes the SLC6A19 (B 0 AT1) neutral amino acid transporter, causes a failure of the transport of neutral (ie, monoaminomonocarboxylic) amino acids in the small intestine and the renal tubules. (medscape.com)
  • Comparative analyses of its 38.5-megabase genome, which encodes 13,210 predicted genes, reveal the species's unique wood-degrading machinery. (nature.com)
  • The cDNA of Danio rerio pol β encodes a protein of 337 amino acids, which is 2-3 amino acids longer than other pol β proteins, and contains a P63D amino acid substitution, unlike mammalian pol βs. (biomedcentral.com)
  • The genes encoding these proteins are highly conserved within the Trichinellids, are constituents of mL1 ESP and localized in the parasite stichosome. (westminster.ac.uk)
  • MicroRNAs (miRNAs) are highly conserved, single-stranded, small non-coding RNAs that have been shown to act as essential regulators of gene expression at the posttranscriptional level. (frontiersin.org)
  • In oomycete pathogens, the N-terminal region of CRN proteins contains a highly conserved LFLAK domain that is characteristic of all CRN proteins. (biomedcentral.com)
  • Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)
  • Activation of NF-κB result in the transcription of various inflammatory genes, including TNF-α and IL-6, to sense microbial pathogens ( 7 - 9 ). (frontiersin.org)
  • The α subunits of protein prenyltransferases consist of tetratricopeptide repeats and are part of the tetratricopeptide repeat superfamily [ 5 ], which also includes functionally diverse proteins involved in transcription, co-chaperoning, protein transport, cell-cycle control and phosphorylation. (biomedcentral.com)
  • Chlamydia trachomatis is an obligate intracellular human pathogen that exhibits stage-specific gene transcription throughout a biphasic developmental cycle. (plos.org)
  • This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
  • Representative σ 66 -dependent gene transcription was repressed in the absence of rsbV1 or upon increased expression of RsbW, and increased upon elevated expression of RsbV1. (plos.org)
  • All of these isoforms are commonly expressed in the kidney, and their potential roles in renal handling of organic anions have been indicated. (aspetjournals.org)
  • Differences between isoforms of an alternatively spliced gene may be subtle or profound. (berkeley.edu)
  • For example, the human Bcl-x gene can be processed to generate several isoforms with markedly different activities. (berkeley.edu)
  • To these ends, several groups have classified and catagorized known alternative isoforms in terms of changes in gene structure between alternative isoforms or by the functional classes of the genes that are involved[ 4 , 16 ]. (berkeley.edu)
  • NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express. (neb.com)
  • The two most common problems during recombinant protein expression are low expression level and solubility of the target protein. (neb.com)
  • Binding response kinetics between Human ACE2 (18-652) Recombinant Protein (hFc-Tag) (immobilized) and SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (in solution, at indicated concentrations). (cellsignal.com)
  • Values on y-axis indicate binding response signals recorded for 4 different concentrations of SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (125, 250, 500 and 1000 nM). (cellsignal.com)
  • The purity of Human ACE2 (18-652) Recombinant Protein (hFc-Tag) was determined by densitometry after SDS-PAGE of 2 μg of protein, followed by staining with Coomassie Blue. (cellsignal.com)
  • Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA-free and Penta·His Antibody, BSA-free are used to detect recombinant protein carrying His tags. (qiagen.com)
  • A subset of high quality and rare information, retrieved and organized by systematic literature- searching tools from existing databases, and related to a subset of genes functioning together in '-omic' sciences. (genomicglossaries.com)
  • NEB also offers solutions for expressing these types of proteins, either in vitro or in vivo. (neb.com)
  • Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
  • We used the in vitro / in vivo dual expression system plasmid, pIVEX, to express Danio rerio pol β ( Danio pol β) for biochemical characterization. (biomedcentral.com)
  • Danio pol β encoded by the in vitro / in vivo -compatible pIVEX plasmid was expressed in E. coli BL21(DE3), BL21(DE3)pLysS, and KRX, and in vitro as a C-terminal His-tagged protein. (biomedcentral.com)
  • [ 4 ] In humans, about 23 different unrelated proteins are known to form amyloid fibrils in vivo. (medscape.com)
  • Nucleotide positions of PAC 454M7 defining preproapelin gene exons (boxes) are shown at the top. (phoenixpeptide.com)
  • Association of nucleotide patterns with gene function classes: application to human 3' untranslated sequences. (auth.gr)
  • Nucleotide sequences located at the ends of EXONS and recognized in pre-messenger RNA by SPLICEOSOMES. (bvsalud.org)
  • A: An alignment of amino acid sequences of rat preproapelin with human and bovine preproapelin. (phoenixpeptide.com)
  • B: An alignment of amino acid sequences of human apelin and angiotensin II. (phoenixpeptide.com)
  • Comparison of the Cry1Ia1 amino acid sequence to known amino acid sequences revealed no significant homology to known toxins or known allergens. (ashs.org)
  • It is the only mushroom-forming fungus for which genes have been inactivated by homologous recombination. (nature.com)
  • Prior information on immune response genes in wild silkmoths is lacking except for a few peripheral studies. (biomedcentral.com)
  • claiming that two homologous chromosomes paired between complementary sequences lead to the formation of a cross-stranded structure that physically links the two component helices. (iucr.org)
  • Following me will be Corinna Tuckey, who will describe the utility and advantages of the PURExpress in vitro protein synthesis kit. (neb.com)
  • Current genome assemblies have several thousands of gaps, causing bad gene model predictions due to missing exons and splice sites. (biomedcentral.com)
  • The only general conclusion that can be drawn from these analyses, however, is that alternative splicing affects genes of nearly every functional class by modifying gene structure in every conceivable way, such as using mutually exclusive exons or alternative donor sites. (berkeley.edu)
  • The gene encoding Sec31B is located on chromosome 10q24 and contains 29 exons. (biologists.com)
  • Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
  • Our results demonstrate that SH3 (Src homology 3) domain-PRM (proline-rich motif) interactions involving multivalent linker proteins play central roles in concentrating NPFs at endocytic sites. (elifesciences.org)
  • Reviewed-Annotation score: -Protein inferred from homology i. (oosteinde.info)
  • Protein phosphatase 1 regulatory subunit 14B is an enzyme that in humans is encoded by the PPP1R14B gene. (wikipedia.org)
  • This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
  • the α subunit of FT and GGT1 is encoded by the same gene, FNTA (see Table 1 ). (biomedcentral.com)
  • Gene structures and chromosomal locations of human protein prenyltransferase subunit genes. (biomedcentral.com)
  • Previous data has reported similarity between human brain and testis gene expression patterns. (karger.com)
  • Hence, it means that human testis could also play a crucial role in human speciation if these two tissues exhibit similar gene expression patterns. (karger.com)
  • Determining the large scale anatomy of gene expression patterns of various human tissues could draw a more convincing conclusion, and better our understanding of the correlation/inter-correlation among different tissues. (karger.com)
  • Among the 17 tissues, the highest similarity in gene expression patterns was between human brain and testis, based on DDD and clustering analysis. (karger.com)
  • Present results provide evidence to support the proposal that human testis and brain share the highest similarity of gene expression patterns. (karger.com)
  • 0 pression patterns of 96 transcripts and prognosis based on a limited representing 80 known genes and 20 13 numbers of markers1-5. (lu.se)
  • Gene-expression patterns used to differentiate four type cause heart damage, and markable finding that genes cur- types of SRBCT. (lu.se)
  • [ 3 ] It is also important to understand that the same polypeptide sequence can produce many different patterns of interresidue or intraresidue interactions. (medscape.com)
  • C: The genomic structure of the human preproapelin gene as found in the human PAC 454M7 clone (GenBank accession no. (phoenixpeptide.com)
  • The complete genomic sequence of the isolate, designated J1 (GenBank accession no. (cdc.gov)
  • In 2004, a causative gene, SLC6A19 (MIM#608893, Genbank accession NM 001003841) , was located on band 5p15.33. (medscape.com)
  • In contrast, the gene sequences and gene maps produced by the public consortium have been deposited regularly within 24 hours of completion in GenBank, a public database set up in the early 1980s when DNA sequencing began, access to which is totally free. (biosafety-info.net)
  • Western blot analysis and biological activity assays showed molecular and functional equivalency between 'SpuntaG2'-derived Cry1Ia1 protein and bacteria-derived Cry1Ia1 protein. (ashs.org)
  • A model is proposed where the relative levels of active antagonist (RsbV1) and switch-protein anti-sigma factor (RsbW) control the availability of σ 66 and subsequently act as a molecular 'throttle' for Chlamydia growth and development. (plos.org)
  • Upstream of the nor and nnrR cluster in Rhodobacter sphaeroides 2.4.3 is a previously uncharacterized gene that has been designated nnrS. (microbiologyresearch.org)
  • Many agricultural species and their pathogens have sequenced genomes and more are in progress. (biomedcentral.com)
  • However, systems biology from functional genomics data is hindered in agricultural species because agricultural genome sequences have relatively poor structural and functional annotation and agricultural research communities are smaller with limited funding compared to many model organism communities. (biomedcentral.com)
  • A relatively large proportion of these genes in these species are electronically predicted. (biomedcentral.com)
  • 2004. Virus-induced gene silencing in Solanum species . (berkeley.edu)
  • Several genes were present as members of gene families, as has also been observed in other insect species. (biomedcentral.com)
  • The extra gene may come from the same species or from a different species. (sciencelearn.org.nz)
  • The Department of Energy Joint Genome Institute has begun sequencing both a male (R40) and a female (GG1) laboratory isolate of the species. (oosteinde.info)
  • Furthermore, other near-human species such as Zeltron, Chiss , and Sullustan share the human ortholog of this locus, which supports the inference that the ancestral sequence was noncoding, and that the GLABR has de novo origins in the Wookiee. (ubc.ca)
  • The IN-His 6 , IN-HA, and IN-2XHA proteins, purified from virus, could be immunoprecipitated with antibodies against His 6 and HA, respectively. (researchwithrutgers.com)
  • For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of anti·His antibodies. Detection of 6xHis-tagged proteins with Anti·His Antibodies (Tetra·His Antibody in the center). A: DHFR; B: DHFR; C: thioredoxin; D: TNF-α; E: TFIIA γ ; F: chaperonin; G DNA polymerase; H: TFIIAαß; for tag location and sequences detected see table 'Proteins detected with QIA express Anti·His Antibodies'. Indicated amounts of pure 6xHis-tagged protein were applied to a nitrocellulose membrane, and detection was carried out with the Anti·His primary antibody indicated diluted 1/2000, followed by chromogenic detection with AP-conjugated rabbit anti-mouse IgG and NBT/BCIP. "> "Sensitivity of QIA express Anti·His Antibodies" ). (qiagen.com)
  • Anti·His Antibodies enable identification of positive expression clones by colony blotting (see figure Identification of positive expression clones by colony blotting. . "> "Identification of positive expression clones by colony blotting" ) and detection of His-tagged thioredoxin (see figure His-tagged thioredoxin detected by Penta·His Antibody in yeast cells. . "> "His-tagged thioredoxin detected by Penta·His Antibody in yeast cells" ). (qiagen.com)
  • For detection, QIA express mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. (qiagen.com)
  • Scientists at AgResearch in New Zealand have successfully produced healthy transgenic cows that make modified milk or human therapeutic proteins in their milk. (sciencelearn.org.nz)
  • Since 2000, scientists at AgResearch have been successfully producing transgenic cows that make modified milk or produce therapeutic proteins to treat human diseases. (sciencelearn.org.nz)
  • Find out more about Transgenic cows making modified milk and Transgenic cows making therapeutic proteins . (sciencelearn.org.nz)
  • The hOAT3 protein was shown to be localized in the basolateral membrane of renal proximal tubules and the hOAT3 gene was determined to be located on the human chromosome 11q12-q13.3 by fluorescent in situ hybridization analysis. (aspetjournals.org)
  • When expressing membrane proteins, researchers must assume from the start that the protein will be difficult to express in functional form. (neb.com)
  • If a membrane protein expresses at a high level, almost certainly some of this protein will be found in the insoluble fraction. (neb.com)
  • As a solution NEB offers a tunable T7 expression strain for the production of membrane proteins and other difficult targets so that the level of functional protein may be optimized. (neb.com)
  • H. pylori attaches and colonizes to the human epithelium using some of their outer membrane proteins (OMPs). (nature.com)
  • Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
  • Outer membrane proteins (OMPs) are distinctive features of Gram-negative bacteria. (nature.com)
  • The recent cloning and sequencing of a gene coding for a membranous progesterone receptor [ 5 ] in African clawed frog ( Xenopus laevis ) brought evidence for the existence of both intracellular and membrane progestin receptors [ 6 ]. (biomedcentral.com)
  • Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
  • The chromosome diagrams and the locations of the genes on the cytogenetic map are according to the NCBI MapViewer [ 67 ]. (biomedcentral.com)
  • The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)
  • These Unigenes represent a wide range of genes classified according to their characterization and function. (karger.com)
  • Once this complex is formed, it is identified via either a ", tag ," attached directly to the antibody, or added in an additional technical step. (englishphonetics.net)
  • 2009). Proteomics and comparative genomic investigations reveal heterogeneity in evolutionary rate of male reproductive proteins in mice (Mus domesticus). (geneticsmr.com)
  • The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
  • Mining of Assembled Expressed Sequence Tag (EST) Data for Protein Families: Application to the G Protein-coupled Receptor Superfamily. (auth.gr)
  • Inactivation of nnrS did not affect nitric oxide metabolism, nor did it affect expression of any of the genes involved in nitric oxide metabolism. (microbiologyresearch.org)
  • Our results show that the GLABR gene is responsible for robust hair growth in Wookiees, and that inactivation of this gene results in reduced androgenic hair growth or hairless phenotype. (ubc.ca)
  • We developed an immunohistochemical (IHC) assay to detect the WUPyV viral protein 1 (WU-VP1) by using an IgG2b designated NN-Ab06. (cdc.gov)
  • The QIA express System provides materials for the expression, purification, detection and assay of 6xHis-tagged proteins. (qiagen.com)
  • ABQ09289) in Escherichia coli from a Gateway pDEST17 plasmid (Life Technologies, Carlsbad, CA, USA) and purifying the protein by using an affinity Ni-NTA column (Pierce Biotechnology, Rockford, IL, USA). (cdc.gov)
  • QP10629 protein expressed in E. Coli. (enquirebio.com)
  • I will start by outlining the most common problems encountered during the expression of recombinant proteins in E. coli and specific solutions will be given for each problem. (neb.com)
  • And then Mehmet Berkman will highlight the SHuffle line of E. coli strains for producing proteins with complex disulphide bonds. (neb.com)
  • A gene can be ordered, it is optimized for E. coli expression and it can be at your lab within three weeks. (neb.com)
  • Finally, we analysed the expression profiles of 15 potential immune-related genes, and the majority of them were induced more prominently with E. coli compared to Micrococcus luteus . (biomedcentral.com)
  • As described in Materials and Methods, we constructed a cDNA library from fat body tissues of E. coli - challenged A. mylitta larvae and randomly sequenced a large number of inserts from the library. (biomedcentral.com)
  • 2005. The R1 resistance gene cluster contains three groups of independently evolving, type I R1 homologues and shows substantial structural variation among haplotypes of Solanum demissum . (berkeley.edu)
  • The human SLC6A19 gene was cloned independently by 2 groups of researchers in 2004. (medscape.com)
  • The human genome has been sequenced separately and independently with major public finance, from the United States and the European Community. (biosafety-info.net)
  • Protein motif recognition in DNA sequences containing indel errors. (auth.gr)
  • There are three different protein prenyltransferases in humans: farnesyltransferase (FT) and geranylgeranyltransferase 1 (GGT1) share the same motif (the CaaX box) around the cysteine in their substrates, and are thus called CaaX prenyltransferases, whereas geranylgeranyltransferase 2 (GGT2, also called Rab geranylgeranyltransferase) recognizes a different motif and is thus called a non-CaaX prenyltransferase [ 1 ]. (biomedcentral.com)
  • Furthermore, based on sequence analysis it is proposed that Hjc_15-6 has a three-part catalytic motif corresponding to E-SD-EVK, and this motif may be common among other Hj-resolving enzymes originating from thermophilic bacteriophages. (iucr.org)
  • 2003. Comparative analyses of potato expressed sequence tag libraries . (berkeley.edu)
  • Our analyses show that at least 32 serine peptidase genes are present in the Bd genome (Additional file 1 ). (biomedcentral.com)
  • therefore, bacterial overexpression was used to produce the protein for kinetic analyses. (biomedcentral.com)
  • IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). (rndsystems.com)
  • miRNAs induce gene degradation and suppression of translation, or both, mainly by imperfect binding to miRNA recognition elements (MREs) within the 3′-untranslated region (UTR) of target mRNAs ( 1 ). (frontiersin.org)
  • We use standardized nomenclature following the Human Genome Organization Gene Nomenclature guidelines and are currently functionally annotating chicken, cow and sheep gene products using the Gene Ontology (GO). (biomedcentral.com)
  • We use experimental data to improve structural annotation of genomes and to functionally characterize gene products. (biomedcentral.com)
  • To test for this, apelin-55 was applied exogenously to HEK293A cells overexpressing proprotein convertase subtilisin kexin 3 (PCSK3), the only apelin processing enzyme identified thus far, and to differentiated 3T3-L1 adipocytes, which endogenously express apelin, PCSK3 and other proprotein convertases. (phoenixpeptide.com)
  • Livestock genomes will always have low build numbers compared with model organisms such as human and mouse and yet they have comparable numbers of genes (UniGene). (biomedcentral.com)
  • 2000). An ordered comparative map of the cattle and human genomes. (geneticsmr.com)
  • Critical to sub-classify SRBCTs, Khan et al .6 used to measure genome-wide gene expres- .com decisions in cancer therapeutics are to microarrays to monitor the expression sion for a variety of biological samples. (lu.se)
  • Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
  • In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
  • Subcellular localization prediction of predicted BoGST proteins was performed using Protcomp 9.0 from Softberry [96]. (molquest.com)
  • Moloney murine leukemia virus (M-MuLV) proviruses carrying integrase (IN) protein tagged either with a simian virus 40 (SV40) nuclear localization signal (NLS) or various antigenic epitopes were generated. (researchwithrutgers.com)
  • Table 1 Structures and predicted subcellular localization of the CRN protein family in Bd JAM 81. (biomedcentral.com)
  • The study would also allow researchers to improve crops by learning how sex-linked genes and reproductive processes could control or enhance plant fertility. (oosteinde.info)
  • Lyophilized GDF7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF-7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). (enquirebio.com)
  • Until early 1990s, renal organic anion transport was thought to be carried out by a few carrier proteins that showed wide substrate specificity. (aspetjournals.org)
  • IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) in an adult prostate and fetal kidney libraries (2). (rndsystems.com)
  • Amino-acid sequence and structure comparison suggested that the enzyme belongs to a group of enzymes classified as archaeal Holliday junction-resolving enzymes, which are typically divalent metal ion-binding dimers that are able to cleave X-shaped dsDNA-Holliday junctions (Hjs). (iucr.org)