Reference: Fluorescence of 2-aminopurine reveals rapid conformational changes in the RB69 DNA polymerase-primer/template complexes upon binding and incorporation of matched deoxynucleoside triphosphates. (neb.com)
With a deoxy-primer/template (dP/T) with 2AP in the n position, a rapid fluorescence quenching occurs within 2 ms, followed by a second, slower fluorescence quenching with a rate constant similar to base incorporation as determined by chemical quench. (neb.com)
Local disruption of DNA structure around the centre of the junction by CCE1 of Saccharomyces cerevisiae has been investigated using 2-aminopurinefluorescence. (dundee.ac.uk)
On binding CCE1, 2-aminopurine bases located at the point of strand exchange exhibit a large increase in fluorescence intensity (up to 39-fold enhancement), consistent with complete unstacking. (dundee.ac.uk)
A stopped-flow method with detection of the fluorescence intensity of 2-aminopurine and pyrrolocytosine located adjacent or opposite to the damage was used. (msu.ru)
2-Aminopurine, a purine analog of guanine and adenine, is a fluorescent molecular marker used in nucleic acid research. (wikipedia.org)
Phosphoramidite for incorporation of a 2-amino-modified purine in a synthetic oligonucleotide. (biosearchtech.com)
It was found that nodal explants were showing new bud generation on MS+ 3.0 mg/L BAP (6-Benzyl amino purine) with generation frequency of 90.00±2. (traditionalmedicines.org)
Inhibitor4
T. cruzi infected mice were treated with an ER stress inhibitor 2-Aminopurine (2AP) during the indeterminate stage and evaluated for cardiac pathophysiology during the subsequent chronic stage. (preprints.org)
Next, cells were treated with protein kinase R inhibitor 2-aminopurine and mitochondrial division inhibitor 1 to examine changes in progesterone levels and expression levels of proteins and mRNAs involved in progesterone biosynthesis. (bvsalud.org)
When this element, designated 2-APRE, is present, splicing becomes sensitive to inhibition by the PKR inhibitor, 2-aminopurine, or by coexpression of transdominant-negative mutant PKR. (huji.ac.il)
In this Letter, we report the coupling of the melamine moiety to 2-hydroxy APA, a potent ornithine decarboxylase inhibitor, with the aim of selectively delivering this compound to the parasite. (elsevierpure.com)
Inhibition1
Inhibition of protein kinase R with 2-aminopurine prevented lipopolysaccharide and polyinosinic:polycytidylic acid induced mitochondrial fission and increased progesterone biosynthesis. (bvsalud.org)
Neighboring bases1
Here, we present a simple and versatile real-time exonuclease assay based on 2-aminopurine, an intrinsically fluorescent nucleotide that is quenched by neighboring bases when embedded in DNA. (edu.sa)
Fluorescent probe2
We have used 2-aminopurine (2AP) as a fluorescent probe in the template strand of a 13/20mer primer/template (D) to detect deoxynucleoside triphosphates (N)-dependent conformational changes exhibited by RB69 DNA polymerase (ED) complexes. (neb.com)
Serra, M. J. Determination Of the Secondary Structure of Group II Bulge Loops Using the Fluorescent Probe 2-Aminopurine. (allegheny.edu)
Guanine2
Bull, G. and Thompson, Katherine (2021) The oxidation of guanine by photoionized 2‑aminopurine . (bbk.ac.uk)
Bull, Graham D. and Thompson, Katherine C. (2018) Proton transfer and tautomerism in 2-aminopurine-thymine and pyrrolocytosine-guanine base pairs . (bbk.ac.uk)
Riboside1
Crespo-Hernández, C. E., "2-Oxopurine Riboside: A Dual Fluorescent Analog and Photosensitizer for RNA/DNA Research", J. Phys. (case.edu)
Oligonucleotide1
2-Aminopurine can substitute for dA in an oligonucleotide. (idtdna.com)
Modification2
Solution studies showed that HX RNA carrying a fluorescent 2-aminopurine modification provides a model system that can be used to monitor ligand binding to both the ribosomal decoding site and, through an indirect effect, the hygromycin B interaction region. (escholarship.org)
2'-O-Methyl RNA is a naturally occurring modification of RNA found in tRNA and other small RNAs that arises as a post-transcriptional modification. (idtdna.com)
Oligo1
Protect your DNA and RNA oligos from nucleases by incorporating 2'-O-Methyl and 2'-Fluoro modifications, or enable "reverse" oligo synthesis. (biosearchtech.com)
Eukaryotic initia1
We report a role for the 3'-untranslated region in control of mRNA splicing and show that human TNF-α 3' UTR harbors a cis- acting element that renders splicing of precursor transcripts dependent on activation of PKR, the RNA-activated protein kinase that phosphorylates eukaryotic initiation factor 2 (eIF2). (huji.ac.il)
Mitochondrial3
Last, effect of 2-aminopurine on mitochondrial fission was determined by immunoblotting and quantitative PCR (qPCR). (bvsalud.org)
Mitochondrial fusion is regulated by the large GTPases mitofusin-1 (Mfn1) and mitofusin-2 (Mfn2), which fuse the OMM, and optic atrophy 1 (OPA-1), which fuses the inner mitochondrial membrane. (bvsalud.org)
Here we review the role of mitochondrial dynamics in health and disease and discuss emerging concepts in the field, such as the role of central versus peripheral fission and the potential role of dynamin 2 (DNM2) as a fission mediator. (bvsalud.org)
Hydrogen1
This modified base can form three hydrogen bonds when base-paired with dT and can increase the Tm of short oligos by as much as 1-2°C per insertion. (idtdna.com)
Synthesis1
In contrast to the continuous leading strand synthesis, the lagging strand is synthesized discontinuously in ~200 nucleotide (nt)-long Okazaki fragments, which are then ligated to form the contiguous lagging strand 2 . (nature.com)
20212
En mars 2021, il est promu professeur titulaire. (epfl.ch)
Modified backbones such as locked nucleic acids (LNA), 2'-O-alkyl-RNA, etc. (nanbiosis.es)
Methyl1
Oligonucleotides can be directly synthesized that contain 2'-O-Methyl RNA. (idtdna.com)
Biology1
Journal of Molecular Biology , 296 (2), 421-433. (dundee.ac.uk)
20222
Environmental Science: Atmospheres 2022 (2), pp. 753-760. (bbk.ac.uk)
M. Paredes-Ramos, E. Conde Piñeiro, H. Pérez-Sánchez, J.M. López-Vilariño, (2022), "Natural peptides as strategy to prevent and reduce the novel SARS-CoV-2 infection", Journal of Food Quality, DOI:10.1155/2022/2102937. (bio-hpc.eu)
Derivatives1
In particular, the P2 aminopurine transporter can also selectively accumulate melamine derivatives. (elsevierpure.com)
Antisense2
Unmodified RNA, RNA with modifications, chimeric oligonucleotides with mixed DNA and RNA bases, 2'-OMe-RNA, 2'-F-RNA, and other antisense RNAs are available at your specific request. (genscript.com)
2'-MOE bases are often used for antisense oligos (ASO), aptamers, and siRNA. (idtdna.com)
Base1
Historically, the first universal base employed was 2'-deoxyInosine (dI). (idtdna.com)
Analysis of changes in gene expression during BVT-14225 varlilumab stimulation of T cells revealed modulation of pro-inflammatory signatures consistent with cellular activation and proliferation, with the IL-2 pathway showing the highest frequency of gene modulation. (bios-mep.info)
Both activities were reduced markedly by treatment of cells with 2- aminopurine but not by genistein. (elsevierpure.com)
Despite its ability to activate PKR during splicing, the 2-APRE within the 3' UTR does not affect translation efficiency of the resulting TNF-α mRNA in transfected cells. (huji.ac.il)
Form1
In one form Low spread Binary Option forex brokers lies directly above the nickel, H satisfy V ~ Ep 2~o (3. (binary-options-guide.com)
Activation2
106/well in 24 well plate) were stimulated in vitro separately with two different protocols: Set 1 (3 donors) was 72?h of continuous costimulation (varlilumab/OKT3), while Set 2 (4 donors) was 46?h of pre-activation with OKT3 followed by 4?h of costimulation (varlilumab and OKT3 or isotype control and OKT3). (bios-mep.info)
Domain analysis revealed an activation domain in the NH 2 -terminal region of p45 and a suppression domain in the basic region-leucine zipper of MarK. (elsevierpure.com)
Type1
En 1996, il est retourné aux Pays-Bas pour joindre le FOM Institute for Atomic and Molecular Physics à Amsterdam où il a mis au point un nouveau type de " streak cameras " pour l infrarouge. (epfl.ch)
Version1
Electronic supplementary material The online version of this article (doi:10.1186/s40425-015-0080-2) contains supplementary material, which is available to authorized users. (bios-mep.info)
Structure1
2-APRE RNA forms a stable, 17-bp stem-loop structure and strongly activates PKR in vitro, inducing eIF2α phosphorylation. (huji.ac.il)