• osteogenic
  • Adult bone marrow mesenchymal stromal cells (MSCs) were derived from the femur of orthopaedic patients, seeded on the scaffolds and cultured under osteogenic induction up to differentiation and mineralization. (mdpi.com)
  • Our laboratory has demonstrated that this distinct embryonic origin of frontal and parietal bones confer differences in embryonic and postnatal osteogenic potential and skeletal regenerative capacity, with frontal neural crest derived osteoblasts benefitting from greater osteogenic potential. (mdpi.com)
  • Much progress has been made in recent years in defining the multiple signaling pathways, which confer osteogenic potential and regenerative capacity on the embryologically disparate calvarial bones. (mdpi.com)
  • bovine
  • Additionally, the majority of cartilage tissue engineering approaches have employed bovine or other animal cells, and tissues grown from these cells are xenogenic. (pubmedcentralcanada.ca)
  • The invention further provides bovine BMP-5 proteins characterized by containing the amino acid sequence set forth in Table I from at least amino acid #42 to amino acid #140 encoded for by the DNA sequence of Table I. These proteins may be further characterized by an apparent molecular weight of 28,000-30,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (google.com)
  • Bovine proteins of the invention may be produced by culturing a cell transformed with a DNA sequence containing the nucleotide sequence the same or substantiallly the same as that shown in Table I from nucleotide #8 through nucleotide #427 and recovering and purifying from the culture medium a proteincontaining the amino acid sequence or a portion thereof as shown in Table I from amino acid #42 to amino acid #140. (google.com)
  • The present invention provides a class of mature native mammalian proteins (the class is termed herein as BCF), represented by native human and bovine BCF described herein, which initiate calcification ~n ~ivo which is important for formation of bone. (google.com)
  • The human and/or bovine BCF can be used to identify and isolate other mammalian BCF proteins which may or may not be homologous (in their nucleotide and amino acid sequences) to human or bovine BCF and which exhibit the BCF biological activity. (google.com)
  • BMP compositions including the human factor and bovine factor thereof, the process of isolating BMP compositions and factors, and the use of such factors and compositions to induce bone formation in animals. (google.ca)
  • This was in agreement with previous data showing chondroitin sulfate GAG chains attached to bovine perlecan produced by chondrocytes and that recombinant human domain I protein was glycosylated with both heparan and chondroitin sulfate chains when expressed in Chinese Hamster Ovary cells. (wikipedia.org)
  • mammalian
  • A phylogenetic analysis of mammalian Smad proteins. (els.net)
  • This invention relates to a cla~s of mature native mammalian proteins which initiates calcification and which is named herein as bone calc~fication factor (BCF). (google.com)
  • In this review we will explain how it has been possible to progress from a deeper understanding of the embryonic origin of the mammalian skull vault to rigorous in vivo and in vitro analysis of the differences in activity of key signaling pathways between the neural crest-derived frontal bones and the paraxial mesoderm-derived parietal bones. (mdpi.com)
  • osteoblasts
  • During a process that replicates normal human fetal development, ~MP-induced osteoblasts form cartilage which, over a period of several months, evolve into solid ~one. (google.com)
  • Modified (flattened) osteoblasts become the lining cells that form a protective layer on the bone surface. (wikipedia.org)
  • Osteoblasts (from the Greek combining forms for "bone", ὀστέο-, osteo- and βλαστάνω, blastanō "germinate") are cells with a single nucleus that synthesize bone. (wikipedia.org)
  • However, in the process of bone formation, osteoblasts function in groups of connected cells. (wikipedia.org)
  • A group of organized osteoblasts together with the bone made by a unit of cells is usually called the osteon. (wikipedia.org)
  • Bone is a dynamic tissue that is constantly being reshaped by osteoblasts, which produce and secrete matrix proteins and transport mineral into the matrix, and osteoclasts, which break down the tissues. (wikipedia.org)
  • Osteoblasts are the major cellular component of bone. (wikipedia.org)
  • Osteoblasts are found in large numbers in the periosteum, the thin connective tissue layer on the outside surface of bones, and in the endosteum. (wikipedia.org)
  • Normally, almost all of the bone matrix, in the air breathing vertebrates, is mineralized by the osteoblasts. (wikipedia.org)
  • During bone formation, the surface layer of osteoblasts consists of cuboidal cells, called active osteoblasts. (wikipedia.org)
  • When the bone-forming unit is not actively synthesizing bone, the surface osteoblasts are flattened and are called inactive osteoblasts. (wikipedia.org)
  • Osteoclasts break down bone tissue, and along with osteoblasts and osteocytes form the structural components of bone. (wikipedia.org)
  • it follows the formation of a first skeleton of cartilage made by chondrocytes, which is then removed and replaced by bone, made by osteoblasts. (wikipedia.org)
  • expression
  • The very essence of an omic approach is to gather complete, comprehensive, systematic knowledge: genomics aims to get complete genome se- quences, microarrays try to monitor the expression of all genes, and proteomic approaches strive to measure all the proteins involved in a biological process. (loginfx.com)
  • 9. Spector JA, Luchs JS, Mehrara BJ, Greenwald JA, Smith LP, Longaker MT: Expression of Bone Morphogenetic Proteins During Membranous Bone Healing. (cornell.edu)
  • Kappa Delta Award for the "Purification, Cloning and Expression of Osteogenin, A Protein Initiator of Bone Differentiation. (wikipedia.org)
  • The core protein mRNA levels of proteoglycans were not affected, thus the decrease in GAG chains was as a result of some other factor, which in this case turned out to be a decrease in expression of sulfate transferase enzymes, which play a key role in GAG biosynthesis. (wikipedia.org)
  • collagen
  • Though the ECM is composed of many intracellularly-synthesized components - including a number of glycosaminoglycans (GAGs) and fibrous proteins such as fibronectin, laminin, collagen, and elastin - it is the latter two fibers that are most influential in defining the mechanical properties of the ECM. (wikipedia.org)
  • Collagen is the fibrous protein that gives the ECM its tensile strength, or rigidity. (wikipedia.org)
  • The mineralised matrix of bone tissue has an organic component of mainly collagen called ossein and an inorganic component of bone mineral made up of various salts. (wikipedia.org)
  • Its matrix is mostly made up of a composite material incorporating the inorganic mineral calcium phosphate in the chemical arrangement termed calcium hydroxylapatite (this is the bone mineral that gives bones their rigidity) and collagen, an elastic protein which improves fracture resistance. (wikipedia.org)
  • The collagen of bone is known as ossein. (wikipedia.org)
  • They synthesize dense, crosslinked collagen and specialized proteins in much smaller quantities, including osteocalcin and osteopontin, which compose the organic matrix of bone. (wikipedia.org)
  • localization
  • Perlecan, a heparan sulfate proteoglycan with preferred localization to vascular basement membranes, is comprised of a ~480 kDa protein core with five distinct domains (I - V). Domains II-V share structural homologies with other protein modules [ 1 ]. (pubmedcentralcanada.ca)
  • genes
  • A targeting vector containing neomycin resistance, herpes simplex virus thymidine kinase and lacZ genes was used to disrupt most of the Nog coding region and some 3' flanking sequence. (jax.org)
  • MSC can be modified to carry therapeutic genes, serving as programmed molecule transmitters to overcome limitations connected with direct injection of beneficial proteins. (biomedcentral.com)
  • If genes do not produce the right proteins in a correct way, a child can have a genetic disorder. (wikipedia.org)
  • wherein
  • 3. The method of claim 1 , wherein said first or second portion of adipose tissue that is removed from said patient is obtained by excisional lipectomy. (google.es)
  • 13. The method of claim 12 , wherein the bone morphogenetic protein is recombinantly generated bone morphogenetic protein. (google.es)
  • 4. The method of claim 3 , wherein said pharmaceutical carrier is hyaluronic acid. (google.ca)
  • 5. The method of claim 3 , wherein the pharmaceutical carrier is a mineral. (google.ca)
  • 3. The process of claim 1 wherein said BMP composition consists essentially of about 7 parts 22-kDa and about 1 part 14-kDa BMP associated proteins, and about 2 parts 18.5 0.5-kDa bBMP factor. (google.ca)
  • Vertebrate
  • Their vertebrate counterparts (bone morphogenetic proteins and Wnt/Wg respectively) function in the formation of vertebrate embryonic hearts as well, suggesting that many of the molecular mechanisms of heart formation are conserved in coelomate animals (Nakamura, 2003). (sunyorange.edu)
  • A bone is a rigid organ that constitutes part of the vertebrate skeleton. (wikipedia.org)
  • defects
  • The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair. (google.com)
  • Bone repair may be necessitated by a number of situations including trauma, congenital defects, pathological deformation or revision surgery [ 1 ]. (mdpi.com)
  • Methods and compositions are provided for the treatment of articular cartilage defects and disease involving the combination of tissue, such as osteochondral grafts, with active growth factor. (google.ca)
  • A freeze dried coprecipitate of BMP with calcium phosphate, made by the aforementioned process of said patent may be implanted in a bone defect caused by injury, old infection, malignancy, and congenital defects. (google.ca)
  • Isolation
  • The isolation, identification and production by recombinant methods of bone calcification factor, a 22KD polypeptide, are disclosed. (google.com)
  • The BCF is provided by isolation from bone sources and by synthesis using recombinant DNA technigues. (google.com)
  • Molecular
  • These proteins may be further characterized by an apparent molecular weight of 28,000-30,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (google.com)
  • Work with BMP material isolated from rabbit dentin matrix protein fraction, using polyacrylamide gel electrophoresis (PAGE) has been assigned a molecular weight of about 23,000. (google.ca)
  • Perlecan consists of a core protein of molecular weight 470 kDa to which three long chains (each approximately 70-100 kDa) of glycosaminoglycans (often heparan sulfate, HS but can be chondroitin sulfate, CS) are attached. (wikipedia.org)
  • human
  • The invention provides human BMP-5 proteins, substantially free from other proteins with which they are co-produced, characterized by containing the amino acid sequence set forth in Table III from amino acid #1 to amino acid #34 encoded for by the DNA sequence of Table III from nucleotide #1 to nucleotide #102. (google.com)
  • By expressing a recombinant form of the N-terminal domain I of the protein and demonstrating that digestion of the peptide with either heparanase or chondroitinase did not lead to complete loss of the peptide's activity, it was shown that chondroitin sulfate chains can be added to human perlecan. (wikipedia.org)
  • In the human body at birth, there are over 270 bones, but many of these fuse together during development, leaving a total of 206 separate bones in the adult, not counting numerous small sesamoid bones. (wikipedia.org)
  • The cortical bone gives bone its smooth, white, and solid appearance, and accounts for 80% of the total bone mass of an adult human skeleton. (wikipedia.org)
  • fracture
  • It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. (wikipedia.org)
  • Ihus ~MP may be useful for replacing bone that has ~een destroyed ~y disease or accident, for use in treatment of scoliosis victims, for treatment of mal- or mis-formed bone, for use in healing of a fracture, etc. (google.com)
  • These compositions and methods may be used in bone fracture healing and repair. (google.com)
  • therapeutic
  • 3. The method of claim 1 , further including the step of adding one or more therapeutic substances to the dose of a BMP. (google.es)
  • 9. The method of claim 7 , further comprising adding one or more therapeutic substances to the dose of a recombinantly generated bone morphogenetic protein. (google.es)
  • 14. The method of claim 12 , further comprising adding one or more therapeutic substances to the dose of a bone morphogenetic protein in contact with the sponge carrier. (google.es)
  • heparan sulfate
  • Although the core protein is important, the large heparan sulfate (HS) chains extending from the core are responsible for most receptor signaling. (wikipedia.org)
  • formation
  • GDF6 has been shown to play an important role in the patterning of the epidermis and bone and joint formation. (wikipedia.org)
  • Injuries to articular cartilage, whether traumatic or from degeneration, generally result in the formation of mechanically inferior fibrocartilage, due to the tissue's poor intrinsic healing response. (pubmedcentralcanada.ca)
  • It is contemplated that these proteins are capable of stimulating promoting, or otherwise inducing cartilage and/or bone formation. (google.com)
  • It is contemplated that these proteins are capable of inducing cartilage and/or bone formation. (google.com)
  • BMP1 protein and procollagen C proteinase (PCP), a secreted metalloprotease requiring calcium and needed for cartilage and bone formation, are identical. (wikipedia.org)
  • The first "flare-up" that leads to the formation of FOP bones usually occurs before the age of 10. (wikipedia.org)
  • Extra bone formation around the rib cage restricts the expansion of lungs and diaphragm causing breathing complications. (wikipedia.org)
  • The balance of bone formation and bone resorption tends to be negative with age, particularly in post-menopausal women, often leading to a loss of bone serious enough to cause fractures, which is called osteoporosis. (wikipedia.org)
  • Intramembranous ossification is the direct ossification of mesenchyme as happens during the formation of the membrane bones of the skull and others. (wikipedia.org)
  • blood vessels
  • Elastin - as its name suggests - is a highly elastic protein with an important role in tissues that need to return to their original positions after deformation, such as skin, blood vessels, and lungs. (wikipedia.org)