OrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesAnthropology, Education, Sociology and Social PhenomenaInformation Science
Random Amplified Polymorphic DNA TechniqueDNA, RecombinantDNA FingerprintingMolecular Sequence DataBase SequenceCloning, MolecularEscherichia coliPlasmids
PolymorphismMarkersAFLPRandomly amplifiedAmplificationRAPD-PCR2000HybridizationSequencesRestrictionStrainsFragmentsMolecularGenesNucleotideAnalysesDiversityProteinsPatternsCultivarsPrimer combinationsASSESSMENTRegionsMethylationSpeciesEnzymeGenomeInvolvesProductsStudiesMethodsBandStudyFutureSamplesTemplateTotalSystemsDouble
Polymorphism7
  • The special cuhivars, "Lidongben", "Nanhu Jiaohe" and "Lizhiben" were named respectively as Lidongben group, Nanhu Jiaohe group, and Lizhiben group, because of their more characteristic DNA polymorphism tested in all respective cuhivars. (jipb.net)
  • These techniques include karyotyping, random amplification of polymorphic DNA, restriction fragment length polymorphism (RFLP), DNA hybridization studies, amplified fragment length polymorphism (AFLP), and polymerase chain reaction (PCR) fingerprinting ( 12 - 17 ). (cdc.gov)
  • PZR ile PZR tabanl olan Rastgele o alt lm Polimorfik DNA (Random Amplified Polymorphic DNA =RAPD) ve Restriction Fragment Length Polymorphism (RFLP) y ntemleriyle et t rlerinin ay r m yap lm t r 11 - 13 . (fusabil.org)
  • The percentage of polymorphic bands was 85% indicates that the population of S. olivacea has high level of polymorphism due to low inbreeding factor within a population. (mabjournal.com)
  • Amplified fragment length polymorphism (AFLP) markers were used to detect the relationships among 12 wild accessions and 62 groundcover chrysanthemum cultivars. (ashs.org)
  • alata ) using amplified fragment length polymorphism (AFLP) technique. (open-science-repository.com)
  • Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Single-Strand Conformation Polymorphism (SSCP) and Microsatellite DNA. (biomedcentral.com)
Markers9
  • Analysis of differentiation (genetic diversity and related relationships) among 22 landrace ( Ficus carica L. sativa ) and 2 wild form ( F. carica L. caprificus ) accessions of fig growing under the same environmental conditions in the Palestinian Fig Collection, Til, Nablus, Palestine, using PCR-based Random Amplified Polymorphic DNA (RAPD) and pomological markers, revealed considerable genetic diversity. (scirp.org)
  • Agarwal, M., Shrivastava, N. and Padth, H. (2008) Advances in Molecular Markers Techniques and Their Applications in Plant Sciences. (scirp.org)
  • This review gives a brief summary on the development of genetic markers including both the classical genetic markers and more advanced DNA-based molecular markers. (biomedcentral.com)
  • By the 1980s, many different types of DNA molecular markers had been explored, e.g. (biomedcentral.com)
  • All of these DNA-based markers contain specific advantages and have played significant roles in the evaluation of genetic diversity in farm animals. (biomedcentral.com)
  • A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
  • 4, 5 The process involves use of many evenly spaced polymorphic DNA markers to create landmarks across each chromosome. (bmj.com)
  • 1984). Solanum demissum, phic DNA (RAPD) and restriction fragment length poly- a Mexican hexaploid species, has been used extensively morphism (RFLP) markers that are closely linked to the in potato breeding programs. (health-abstracts.com)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
AFLP1
Randomly amplified2
Amplification2
  • The RAPD (Random Amplification Polymorphic DNA) allows the amplification of DNA sequences and is a simple and quick technique that does not require prior knowledge on the genomes to characterize organisms, using one randomly determined (usually a decamer) primer 17 . (bvsalud.org)
  • Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
RAPD-PCR1
  • Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine the genetic variability among individuals of S . olivacea from Setiu Wetlands, Terengganu. (mabjournal.com)
20001
  • 2000). The main advantage in this context is that the complex genetic and epigenetic correlations among interconnected parts of the brain can be explored using complementary molecular, developmental, structural, pharmacological, and behavioral techniques. (nervenet.org)
Hybridization3
  • This has been done using techniques such as polymerase chain reaction, gene restriction, use of probe hybridization and also using primers. (australiaessays.com)
  • In this case, the basic principle is to detect specific DNA and RNA sequences via hybridization of a probe, detecting any complementary sequence on the cDNA of this bacterium. (australiaessays.com)
  • Classification of Trypanosomatids from insects and plants by the UP-PCR (universally primed PCR) technique and cross dot blot hybridization of PCR products. (microbiologyresearch.org)
Sequences4
  • Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
  • We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
  • DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. (lookformedical.com)
  • Cis-acting DNA sequences which can increase transcription of genes. (lookformedical.com)
Restriction1
  • Ribosomal DNA restriction analysis and synthetic oligonucleotide probing in the identification of genera of lower trypanosomatids. (microbiologyresearch.org)
Strains3
  • Effect of Development Stage on the Artemisinin Content and the Sequence Characterized Amplified Region (SCAR) Marker of High-Artemisinin Yielding Strains of Artemisia annua L. [J]. J Integr Plant Biol. (jipb.net)
  • With the release of a genome wide SNPs database, it has become feasible to analyse these genetically determined QTLs for genes polymorphic in these strains. (bmj.com)
  • To discriminate P. falciparum strains, the sizes of amplified products of these genes are compared, routinely based on migration patterns with agarose-gel electrophoresis because of ease of use and low cost. (biomedcentral.com)
Fragments3
  • Twenty reproducible RAPD fragments generated by OPA 08 and OPA 10 with 17 fragments were polymorphic. (mabjournal.com)
  • A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. (scielo.br)
  • The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. (scielo.br)
Molecular8
  • Both techniques grouped all isolates into eight previously established molecular types. (cdc.gov)
  • Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Ecology. (mabjournal.com)
  • Conclusions: API 20C AUX presented the best agreement with molecular random identification and CHROMagar showed good agreement for C. albicans, C. tropicalis, C. dubliniensis and C. krusei identification. (bvsalud.org)
  • A caracterização molecular foi baseada em marcadores RAPD, sequenciamento da região do espaçador interno transcrito do DNA ribossomal e "fingerprinting" telomérico. (scielo.br)
  • These diagnostic methods are at the cellular and molecular level since both DNA and proteins are major components of a cell. (australiaessays.com)
  • At present, DNA molecular marker techniques are widely applied in the fields of germplasm identification, phylogenetics, and genetic structural analysis. (biomedcentral.com)
  • These species share a high sequence homology of 16S rDNA and several molecular techniques already applied to discriminate among them give ambiguous results. (biomedcentral.com)
  • In the last years several molecular techniques have been proposed in order to identify bifidobacteria. (biomedcentral.com)
Genes2
  • Both gel and capillary methods evaluated polymorphic regions of the merozoite surface protein 1 and 2 and glutamine rich protein genes. (biomedcentral.com)
  • Most commonly, genotyping methods take advantage of variation in highly polymorphic genes of Plasmodium falciparum , including merozoite surface proteins 1 and 2( msp1 and msp2 ) and glutamine-rich protein ( glurp ) [ 2 ]. (biomedcentral.com)
Nucleotide1
  • An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. (lookformedical.com)
Analyses1
  • In order to find those anatomical characters that statistically discriminate among higher basommatophoran taxa, we performed discriminant analyses (DA) with DNA lineages as a grouping variable. (malacsoc.org.uk)
Diversity1
  • Mitochondrial DNA diversity of mud crab Scylla olivacea (Portunidae) in Peninsular Malaysia: a preliminary assessment. (mabjournal.com)
Proteins2
Patterns1
  • Genomics and changes associated with gene patterns have enabled the prediction of etiological factors leading to a disease being shed light on genetic mapping and prognosis on alterations of DNA in novel diseases. (australiaessays.com)
Cultivars2
  • The results indicate that the application of RAPD technique in the taxonomic study has gained some resolution which could not be obtained by morphological analysis and has provided available data for developing longan production and for breeding new longan cultivars. (jipb.net)
  • Random amplified polymorphic DNA has proven useful in sorting out the relationships of the C. pepo species, varieties, and cultivars, showing that few, if any, modern cultivars have their origins with C. texana. (wikipedia.org)
Primer combinations2
  • Nineteen EcoR I/ Mse I primer combinations revealed 452 informative polymorphic bands with a mean of 23.8 bands and 71.5% polymorphic rate per primer pair. (ashs.org)
  • Three primer combinations that produced clear scorable and highly polymorphic bands were screened from 64 Eco RI and Ms eI primer combinations. (open-science-repository.com)
ASSESSMENT1
  • Provision of broad, method-based PT schemes, such as DNA sequencing, would allow assessment of a large number of tests for which formal PT is not currently available. (cdc.gov)
Regions1
  • Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. (lookformedical.com)
Methylation1
  • Stress Treatments and DNA Methylation Affected the Somatic Embryogenesis of Citrus Callus [J]. J Integr Plant Biol. (jipb.net)
Species2
  • Aim: To measure the agreement of methods for identification of Candida species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques. (bvsalud.org)
  • The developed PCR-RFLP technique resulted in efficient discrimination of the tested species and subspecies and allowed the construction of a dichotomous key in order to differentiate the most widely distributed Bifidobacterium species as well as the subspecies belonging to B. pseudolongum and B. animalis . (biomedcentral.com)
Enzyme2
  • DNA samples (10 µg) were digested with Eco Rl, Hin dlll, Eco RV, These three BC populations are designated 1K6, 1K27 and LB1, or Dra l at a DNA/enzyme ratio of 7 units per microgram. (health-abstracts.com)
  • The enzyme has a preference for double-stranded DNA. (lookformedical.com)
Genome2
  • Genome sequence of Sclerotinia sclerotiorum hypovirulence-associated DNA virus-1 found in the fungus Penicillium olsonii isolated from Washington state, USA. (usda.gov)
  • In addition, with biotechnological and computer innovations, novel strategies such as whole-genome SNP chips and DNA Barcoding have emerged. (biomedcentral.com)
Involves1
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Products2
  • 1998). These last experiments showed that products were independent of the kind of irradiating particles. (hsp-signal.com)
  • This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. (lookformedical.com)
Studies1
  • 80] present a pilot clinical study evaluating the feasibility of endoscopic transluminal omental patch closure for perforated peptic selleck chemicals llc ulcers, with the hypothesis that the technique will be successful at closing ulcer perforations, as evidenced by intraoperative leak test and post operative water-soluble contrast studies. (hsp-signal.com)
Methods2
  • Methods: Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. (bvsalud.org)
  • In recent decades, traditional methods of microorganism phenotyping have been replaced or added by the procedures associated to recombinant DNA 12-14 . (bvsalud.org)
Band1
  • Her bir t r i in en az 2 adet DNA band olu turan primerler pozitif (+), 2 adetten az DNA band olu turan primerler negatif (-) olarak de erlendirildi (tablo 2 ). (fusabil.org)
Study1
  • 1998). For convenience, the gel (Diversified Biotech, Boston, Mass.) 0.6% agarose gel, stained clones of S. bulbocastanum and S. tuberosum used in this study with ethidium bromide and visualized on a UV transilluminator. (health-abstracts.com)
Future1
  • Other techniques and future developments Self-expandable metal stents Primary stenting and drainage has been shown to be an effective and safe way to treat esophageal perforations or anastomotic leaks after gastric bypass surgery. (hsp-signal.com)
Samples1
  • The genomic DNA was extracted from ten samples of mud crab by using DNeasy kit. (mabjournal.com)
Template3
  • This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
  • The biosynthesis of RNA carried out on a template of DNA. (lookformedical.com)
  • The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. (lookformedical.com)
Total1
  • Foi usado um total de 78 primers RAPD, sendo que 8 apresentaram 54% de fragmentos de DNA polimórficos. (scielo.br)
Systems1
  • Seed lot culture maintenance techniques (seed-lot systems) are used so that the viable micro-organisms used for inoculation are not more than 5 passages removed from the original master seed-lot. (top5binarybrokers.com)
Double1
  • DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). (lookformedical.com)