• synthesis
  • The physiological and biochemical signals that induce stress protein (HSP) synthesis remain conjectural. (physiology.org)
  • Ehrlich tumor cells ( 11 ), murine cell lines ( 30 ), and rat cardiomyocytes ( 34 ) also demonstrated increased synthesis of HSP70 in response to ATP depletion arising from metabolic inhibition. (physiology.org)
  • We previously showed that thermal stress causes an induction of HSP70 synthesis in these cells, whereas severe hypoxia does not ( 9 ). (physiology.org)
  • BiP is an abundant protein under all growth conditions, but its synthesis is markedly induced under conditions that lead to the accumulation of unfolded polypeptides in the ER. (wikipedia.org)
  • When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. (wikipedia.org)
  • 1995
  • Arnold, D., Faath, S., Rammensee, H. and Schild, H. (1995) Cross-priming of minor histocompatibility antigen-specific cytotoxic T cells upon immunization with the heat shock protein gp96. (springer.com)
  • 2000
  • Basu, S., Binder, R. J., Suto, R., Anderson, K. M. and Srivastava, P. K. (2000) Necrotic but not apoptotic cell death releases heat shock proteins, which deliver a partial maturation signal to dendritic cells and activate the NF-kappa B pathway. (springer.com)
  • Binder, R. J., Han, D. K. and Srivastava, P. K. (2000) CD91: a receptor for heat shock protein gp96. (springer.com)
  • mRNA
  • Heat shock (25°C) did not significantly affect ATP levels but resulted in an increase in HSP70 mRNA. (physiology.org)
  • In contrast, hsp70-1 mRNA levels increased gradually and to the same extent in response to heat shock in both populations. (biologists.org)
  • Hsc70 mRNA levels were significantly elevated by heat shock in southern fish, but not in northern fish. (biologists.org)
  • FourU are found in the 5' untranslated region of the gene for heat shock protein Salmonella agsA, they repress translation of this protein by base-pairing the Shine-Dalgardo sequence of the gene's mRNA. (wikipedia.org)
  • Among 8 primary translation products of Sendai virus P/C mRNA, leaky scanning accounts for translation of protein C', P, and C proteins, while expression of protein Y1 and Y2 is initiated via a ribosomal shunt discontinuous scanning. (wikipedia.org)
  • In the case of adenovirus late mRNA and hsp70 mRNA, instead of recognition of stop codon of first short ORF, pausing of translation is caused by scanning ribosome with three conserved sequences that are complementary to the 3' hairpin of 18S ribosomal RNA. (wikipedia.org)
  • cellular
  • Phylogenetic trees constructed by two independent methods indicate that ancient molecular and cellular events have given rise to at least four monophyletic groups of eukaryotic HSP70 proteins. (springer.com)
  • DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. (genecards.org)
  • Heat shock refers to cellular exposure to rapid stress changes such as temperature, toxins, oxidative stress, heavy metals, and pathogenic infections. (wikipedia.org)
  • Other cellular damages induced by heat shock include cytoskeletal rearrangement, changes in organelle localization, decrease in ATP production, unsafe drop in cellular pH levels, decreased translation of proteins, and changes in RNA splicing. (wikipedia.org)
  • This study demonstrates that a reduction in the cellular energy status will not induce stress protein gene transcription in rainbow trout red blood cells and may, in fact, limit induction during extreme metabolic inhibition. (physiology.org)
  • The heat shock response (HSR) is a cellular stress response that senses protein misfolding and restores protein folding homeostasis, or proteostasis. (g3journal.org)
  • Together, these data indicate that the specific protein folding requirements from the unique cellular proteomes sensitizes each tissue to disruption of distinct subsets of the proteostasis network. (g3journal.org)
  • High-throughput methods are provided for quantitatively measuring the modulation of heat shock protein (HSP) expression in a cell by exposing the cell to at least one stress and measuring cellular stress responses. (patentsencyclopedia.com)
  • Chaperones stabilize new proteins during translation, mature proteins which are partially unstable but also proteins that have become partially denatured due to various kinds of cellular stress. (wikipedia.org)
  • Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation. (wikipedia.org)
  • The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. (wikipedia.org)
  • Late adenovirus mRNAs contains a 5' tripartite leader, a highly conserved 200-nt NTR with a 25- to 44- nt unstructured 5' conformation followed by a complex group of stable hairpin structure, which confers preferential translation by reducing the requirement for the eIF-4F (cap-binding protein complex), which is inactivated by adenovirus to interfere cellular protein translation. (wikipedia.org)
  • amino
  • proteins from the most distantly related species share at least 45% identity and more than one-sixth of the amino acids are identical in the aligned region (567 amino acids) among all proteins analyzed. (springer.com)
  • This family of proteins contain a 70 amino acid consensus sequence known as the J domain. (wikipedia.org)
  • There were no fixed differences in amino acid sequence between populations in either hsp70-1 or hsp70-2 , and only a single conservative substitution between populations in hsc70 . (biologists.org)
  • highly conserved
  • Like other steroid nuclear receptors, the full-length protein, isoform B, can be divided into 4 functional regions, namely a variable N-terminal region followed by a highly conserved DNA-binding domain, variable hinge region and moderately conserved ligand binding domain. (wikipedia.org)
  • eukaryotic
  • Eukaryotic genomes encode multiple 70-kDa heat-shock proteins (HSP70s). (springer.com)
  • The analyses indicate that the major eukaryotic HSP70 groups arose prior to the divergence of the earliest eukaryotes, roughly 2 billion years ago. (springer.com)
  • This subfamily appears to have resulted from an ancient gene duplication that occurred at approximately the same time as the origin of the major eukaryotic HSP70 groups. (springer.com)
  • stress
  • Most heat shock proteins (Hsp) function as molecular chaperones that help organisms to cope with stress of both an internal and external nature. (wiley.com)
  • Here, we review the recent evidence of the relationship between stress resistance and inducible Hsp expression, including a characterization of factors that induce the heat shock response and a discussion of the associated costs. (wiley.com)
  • It has also been hypothesized that during metabolic stress (i.e., decrease in ATP), the constitutive and inducible forms of HSP70 become stably bound with proteins that are unable to fold or have become denatured. (physiology.org)
  • Nonetheless, effects of these mutations can still manifest when Hsp70 chaperones are overwhelmed during stress conditions. (wikipedia.org)
  • homologues
  • HSP70s localized in mitochondria and plastids are most similar to the DnaK HSP70 homologues in purple bacteria and cyanobacteria, respectively, which is consistent with the proposed prokaryotic origin of these organelles. (springer.com)
  • stimulate
  • It has a potential to stimulate Hsp70 expression, with overexpression of Hsp70 being found to prevent the development of inflammatory process in the large intestinal mucosa provoked by various damaging factors. (wikipedia.org)
  • 1996
  • Ribosome shunting process was first discovered in CaMV in 1993, and then was reported in Rice tungro bacilliform virus (RTBV) at 1996. (wikipedia.org)
  • induce
  • Inhibition of oxidative phosphorylation and glycolysis with azide and iodoacetic acid respectively, decreased ATP by 79% and prevented lactate production, but did not induce either HSP70 or HSP30 gene transcription in these cells. (physiology.org)
  • disulfide isomerase
  • Barbouche, R., Miquelis, R., Jones, I. M. and Fenouillet, E. (2003) Protein-disulfide isomerase-mediated reduction of two disulfide bonds of HIV envelope glycoprotein 120 occurs post-CXCR4 binding and is required for fusion. (springer.com)
  • Cells
  • Heat shock proteins are also believed to play a role in the presentation of pieces of proteins (or peptides) on the cell surface to help the immune system recognize diseased cells. (wikipedia.org)
  • As the available HSP70 levels decline, the cells respond by increasing HSP expression through a feedback mechanism yet to be described ( 3 , 5 , 29 ). (physiology.org)
  • ATP depletion was shown to be sufficient to activate the heat shock response in HeLa cells ( 24 ) and C2C12 myogenic cells ( 5 ). (physiology.org)
  • Furthermore, this protein enhances antigen-specific tumor immunity by facilitating more efficient antigen presentation to cytotoxic T cells. (wikipedia.org)
  • peptide
  • SBDβ serves as a binding pocket for client proteins or peptide and SBDα serves as a helical lid to cover the binding pocket. (wikipedia.org)
  • induction
  • however dietary phosphorus deficiency caused organ specific induction of HSP70 in catla fingerlings. (springer.com)
  • Both northern and southern killifish significantly increased hsp70-2 levels above control values ( T on ) at a heat shock temperature of 33°C, but the magnitude of this induction was greater in northern fish, suggesting that northern fish may be more susceptible to thermal damage than are southern fish. (biologists.org)
  • The distal HSE is required for heat shock induction and the proximal HSE functions as a permissive enhancer. (wikipedia.org)
  • human heat-shock p
  • Peripheral blood leukocytes were stimulated with human cytomegalovirus (HCMV), Chlamydia pneumoniae , human heat-shock protein 60 (hHSP60), or oxidized LDL (ox-LDL). (ahajournals.org)
  • Several antigens, in particular Chlamydia pneumoniae , human cytomegalovirus (HCMV), oxidized LDL (ox-LDL), and human heat-shock protein-60 (hHSP60), have been implicated in the pathogenesis of coronary artery disease. (ahajournals.org)
  • Biochemistry
  • In biochemistry, heat shock is the effect of subjecting a cell to a temperature that is greater than the optimal temperature range of function of the cell. (wikipedia.org)