• cellular
  • The intestinal dysplasia resulted from an increase in progenitor cell population and the upregulation of β-catenin transcription through the inhibition of cellular differentiation. (wikipedia.org)
  • 12 ]). Members of this family are involved in a variety of cellular processes, ranging from house-keeping gene function (Oct-1) to programming of embryonic stem cells (Oct-4), development of the immune system (Oct-1 and Oct-2), of the pituitary gland (Pit-1) or of the nervous system (Brn-1 through -4 and Oct-6). (biomedcentral.com)
  • Physical binding to the FR by the cellular transcription factors Oct-1 and Oct-2 was demonstrated by using an electrophoretic mobility-shift assay. (microbiologyresearch.org)
  • recombination
  • Before Ig class switching, RNA transcription through the specific S regions undergoing recombination is induced by cytokines and other activators that induce and direct switching. (jimmunol.org)
  • Deletions of various portions of the I exons and their upstream regulatory elements by gene targeting experiments have demonstrated that GL transcripts are required for switch recombination ( 6 , 7 , 8 , 9 , 10 ). (jimmunol.org)
  • induces
  • TGF-β1 induces GL α transcripts and directs class switching to IgA in mouse splenic B cells activated by polyclonal activators, such as LPS ( 6 , 7 ). (jimmunol.org)
  • expression
  • Ectopic expression of the factor in adult mice has been found to cause the formation of dysplastic lesions of the skin and intestine. (wikipedia.org)
  • Oct-6 expression is described to be confined to the developing brain, Schwann cells, oligodendrocyte precursors, testes, and skin. (biomedcentral.com)
  • Our data show that Oct-6 expression is not as restricted as previously assumed. (biomedcentral.com)
  • It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. (wikipedia.org)
  • Positioning of H2A.Z containing nucleosomes around transcription start sites has now been shown to affect the downstream gene expression. (wikipedia.org)
  • polymerase
  • 1. Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. (wikipedia.org)
  • nucleosome
  • These results indicate a role for Vps75 in nucleosome dynamics during transcription, and importantly, this function appears to be largely independent of Rtt109. (stanford.edu)
  • In particular, we discuss how NER factors and central players in chromatin dynamics such as histone modifiers, nucleosome remodeling factors, and histone chaperones function to mobilize histones during repair. (mdpi.com)
  • initiation
  • Histones modifications in chromatin were analyzed at various time intervals (along a 6-day period) following the initiation of in vitro embryonic stem cell differentiation. (wikipedia.org)
  • The process begins with the assembly of general transcription factors around the transcription start site (TSS) to form the pre-initiation complex (PIC), which is completed with the recruitment of Pol II. (mdpi.com)
  • distinct
  • We and others have shown that TGCTs have a unique DNA methylation profile distinct from that of somatic tissue-derived neoplasms ( 3 - 6 ). (aacrjournals.org)
  • base pairs
  • Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins in 1974, and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. (wikipedia.org)
  • Cell
  • Gene knockdown of Oct-4 promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal. (wikipedia.org)
  • Oct-4 is one of the transcription factors used to create induced pluripotent stem cells, together with Sox2, Klf4 and often c-Myc in mouse, demonstrating its capacity to induce an embryonic stem cell-like state. (wikipedia.org)
  • 5 ]). LIF was thus characterized as a pleiotropic cytokine with pro or anti-differentiation, pro or anti-survival effects depending upon cell maturity and cell types [ 6 , 7 ]. (pubmedcentralcanada.ca)
  • 1977-1979: Roeder develops cell-free systems to better study transcription. (wikipedia.org)
  • Composed of the purified RNA polymerases and components extracted from cell nuclei, the systems allow researchers to recreate transcription in a test tube in a way that faithfully mimics the real process in cells. (wikipedia.org)
  • complex
  • Glycosylated LIF can also hold mannose phosphate residues able to bind the Mannose 6 phosphate receptor to the core gp130/GP190 complex and allowing recycling of LIF ligand [ 16 ]. (pubmedcentralcanada.ca)
  • human
  • This effect has also been seen with the Thomson reprogramming factors, reverting human fibroblast cells to iPSCs through Oct-4, along with Sox2, Nanog, and Lin28. (wikipedia.org)
  • 6. A composition according to claim 3 wherein the presence in said plasmid of multiple copies of an origin of replication facilitates efficient replication of said plasmid in human cells. (google.com)
  • inhibits
  • Differentiation was triggered by the removal of Leukemia inhibitory factor (LIF) which inhibits differentiation. (wikipedia.org)
  • mutations
  • encoding GAGA factor, brm and polybromo / bap180 mutations compromise the H3.3 replacement and boundary functions in a synergistic manner. (biologists.org)
  • stem cells
  • It was later deterimined that only two of these four factors, Oct4 and Klf4 were sufficient to reprogram mouse adult neural stem cells. (wikipedia.org)