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  • genes
  • Furthermore, Vps75 is recruited to activated genes in an Rtt109-independent manner, and its genome-wide association with genes correlates with transcription rate. (stanford.edu)
  • Interestingly, histone H2B dynamics at some of these genes are consistent with a role for Vps75 in histone H2A/H2B eviction/deposition during transcription. (stanford.edu)
  • These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. (wikipedia.org)
  • When this month's Pillars of Immunology paper was published in 1986 ( 1 ), it was clear that cis -acting motifs remote from the RNA start site were critical for the transcription of an expanding list of viral, prokaryotic, and eukaryotic genes. (jimmunol.org)
  • Supporting this as the mechanism of enhancer activity in mammalian genes, several DNase footprinting and competition studies pointed to the existence of similar enhancer-specific trans -activating factors ( 2 , 3 ). (jimmunol.org)
  • Furthermore, motifs including the Sp1-binding site were identified as constitutive elements contributing to the transcription of some eukaryotic genes ( 2 , 3 ). (jimmunol.org)
  • Several laboratories had identified regulatory elements within the Ig H and L chain genes including downstream enhancers located within introns in both the μ H and κ L chain loci ( 1 , 2 , 6 ). (jimmunol.org)
  • This factor (named IgNF-A and later identified as Oct-1) was also found in HeLa cells, and its consensus binding sequence was functional in the promoters of several vertebrate small nuclear RNA (snRNA) genes ( 18 ). (jimmunol.org)
  • Furthermore, genes are often flanked by several binding sites for distinct transcription factors, and efficient expression of each of these genes requires the cooperative action of several different transcription factors (see, for example, hepatocyte nuclear factors). (wikipedia.org)
  • Other transcription factors differentially regulate the expression of various genes by binding to enhancer regions of DNA adjacent to regulated genes. (wikipedia.org)
  • enhancer
  • Despite this understanding, exactly how these elements exerted their "enhancer effects" to regulate eukaryotic gene transcription was not known. (jimmunol.org)
  • One exception was a factor bound to the third fragment (κ3) of κE that was not competed away by cold probes from any other region but was competed for by the SV40 enhancer (Fig. 5 of Ref. 1 ). (jimmunol.org)
  • Schwann
  • Oct-6 expression is described to be confined to the developing brain, Schwann cells, oligodendrocyte precursors, testes, and skin. (biomedcentral.com)
  • differentiation
  • Gene knockdown of Oct-4 promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal. (wikipedia.org)
  • The intestinal dysplasia resulted from an increase in progenitor cell population and the upregulation of β-catenin transcription through the inhibition of cellular differentiation. (wikipedia.org)
  • Histones modifications in chromatin were analyzed at various time intervals (along a 6-day period) following the initiation of in vitro embryonic stem cell differentiation. (wikipedia.org)
  • Differentiation was triggered by the removal of Leukemia inhibitory factor (LIF) which inhibits differentiation. (wikipedia.org)
  • interacts
  • Many of these GTFs do not actually bind DNA, but rather are part of the large transcription preinitiation complex that interacts with RNA polymerase directly. (wikipedia.org)
  • expression
  • Ectopic expression of the factor in adult mice has been found to cause the formation of dysplastic lesions of the skin and intestine. (wikipedia.org)
  • Our data show that Oct-6 expression is not as restricted as previously assumed. (biomedcentral.com)
  • Positioning of H2A.Z containing nucleosomes around transcription start sites has now been shown to affect the downstream gene expression. (wikipedia.org)
  • nucleosome
  • These results indicate a role for Vps75 in nucleosome dynamics during transcription, and importantly, this function appears to be largely independent of Rtt109. (stanford.edu)
  • In particular, we discuss how NER factors and central players in chromatin dynamics such as histone modifiers, nucleosome remodeling factors, and histone chaperones function to mobilize histones during repair. (mdpi.com)
  • activators
  • TGF-β1 induces GL α transcripts and directs class switching to IgA in mouse splenic B cells activated by polyclonal activators, such as LPS ( 6 , 7 ). (jimmunol.org)
  • cellular
  • 12 ]). Members of this family are involved in a variety of cellular processes, ranging from house-keeping gene function (Oct-1) to programming of embryonic stem cells (Oct-4), development of the immune system (Oct-1 and Oct-2), of the pituitary gland (Pit-1) or of the nervous system (Brn-1 through -4 and Oct-6). (biomedcentral.com)
  • replication
  • 6. A composition according to claim 3 wherein the presence in said plasmid of multiple copies of an origin of replication facilitates efficient replication of said plasmid in human cells. (google.com)
  • DNA replication, transcription, and repair) that necessarily disorganize-at least transiently-chromatin structure. (mdpi.com)
  • embryonic
  • Oct-4 is one of the transcription factors used to create induced pluripotent stem cells, together with Sox2, Klf4 and often c-Myc in mouse, demonstrating its capacity to induce an embryonic stem cell-like state. (wikipedia.org)
  • distinct
  • We and others have shown that TGCTs have a unique DNA methylation profile distinct from that of somatic tissue-derived neoplasms ( 3 - 6 ). (aacrjournals.org)
  • Oct4
  • It was later deterimined that only two of these four factors, Oct4 and Klf4 were sufficient to reprogram mouse adult neural stem cells. (wikipedia.org)
  • base pairs
  • Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins in 1974, and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. (wikipedia.org)
  • Sox2
  • This effect has also been seen with the Thomson reprogramming factors, reverting human fibroblast cells to iPSCs through Oct-4, along with Sox2, Nanog, and Lin28. (wikipedia.org)
  • coactivator
  • 1996: Roeder's laboratory discovers the major conduit for communication between gene-specific activators and the general transcription machinery in animal cells: a giant coactivator (TRAP/SMCC) that consists of about 25 different protein chains and is referred to as the human mediator after its counterpart in yeast. (wikipedia.org)
  • MOLECULAR AND CEL
  • Functional interaction between the POU domain protein Tst-1/Oct-6 and the high-mobility-group protein HMG-I/Y". Molecular and Cellular Biology. (wikipedia.org)
  • binds
  • We first tested CXXC finger protein 1 (Cfp1), which binds to non-methylated CpG dinucleotides in vitro by a CXXC zinc finger domain 6 , 12 . (pubmedcentralcanada.ca)
  • Genetic
  • These studies highlight a need to develop more potent vaccines to overcome developmental and other factors, such as genetic inheritance, in mounting adaptive immunity. (stanford.edu)
  • cell
  • This protein shares an extended region of sequence similarity, called the POU domain, with the lymphoid-specific transcription factor Oct-2, the pituitary-specific transcription factor Pit-1, and the Caenorhabditis elegans cell lineage gene product unc-86. (cshl.edu)
  • Stem cell homeostasis is maintained through epigenetic mechanisms that are highly dynamic in regulating the chromatin structure as well as specific gene transcription programs. (wikipedia.org)
  • The transcription factors Oct-4, Sox2 and Nanog are part of a complex regulatory network with Oct-4 and Sox2 capable of directly regulating Nanog by binding to its promoter, and are essential for maintaining the self-renewing undifferentiated state of the inner cell mass of the blastocyst, embryonic stem cell lines (which are cell lines derived from the inner cell mass), and induced pluripotent stem (iPS) cells. (wikipedia.org)
  • Oct-4 is one of the transcription factors used to create induced pluripotent stem cells, together with Sox2, Klf4 and often c-Myc in mouse, demonstrating its capacity to induce an embryonic stem cell-like state. (wikipedia.org)
  • 1977-1979: Roeder develops cell-free systems to better study transcription. (wikipedia.org)
  • Composed of the purified RNA polymerases and components extracted from cell nuclei, the systems allow researchers to recreate transcription in a test tube in a way that faithfully mimics the real process in cells. (wikipedia.org)
  • mutations
  • encoding GAGA factor, brm and polybromo / bap180 mutations compromise the H3.3 replacement and boundary functions in a synergistic manner. (biologists.org)