The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. (wikipedia.org)
A Stanford University group led by George Stark that includes James Alwin and David Kemp developed a similar technique in 1977. (thedjournal.com)
1977, According to the method of James Alwine, David Kemp and George Stank. (chmlab.com)
Electrophoresis2
Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. (wikipedia.org)
Strictly speaking, the term 'northern blot' refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. (wikipedia.org)
Radioactive1
Western Blot: Detection by radioactive or chemical marker. (chmlab.com)
Gene4
The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. (wikipedia.org)
With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and morphogenesis, as well as in abnormal or diseased conditions. (wikipedia.org)
To create controls for comparison in a northern blot, samples not displaying the gene product of interest can be used after determination by microarrays or RT-PCR. (wikipedia.org)
Southern nalyses at medium and high stringency indicated that G. cingulata possesses ne gene for the secreted aspartic proteinase, and Northern blots indicated that gene expression was induced by exogenous protein and repressed by ammonium salts. (microbiologyresearch.org)
Western8
The western blotting technique today is a staple of DNA analysis and is performed in labs worldwide. (thedjournal.com)
Burnette had been aware of the Southern blot and sought to honor it, and the location of his work, by naming his technique the western blot. (thedjournal.com)
Despite that rejection, Burnette's western blotting technique quickly became popular, thanks to the distribution of pre-prints to friends, who replicated the process repeatedly. (thedjournal.com)
The naming conventions were hardly the only controversy regarding western blotting . (thedjournal.com)
Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross-reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
These cells were characterised by PCR, immunocytochemistry, electron microscopy, and Western and Northern blot analyses. (ersjournals.com)
At this time, the territory stretching in the western sea-hoard from Chilaw through Puttalam to Mannar and thence to the Northern Regions and in the East, Trincomalee and also the Batticaloa Regions that extended southwards up to Kumana or to the northern banks of the river Kumbukkan Oya were firmly established as the exclusive homeland of the Tamils. (sangam.org)
Annealing temperature1
The transfer buffer used for the blotting usually contains formamide because it lowers the annealing temperature of the probe-RNA interaction, thus eliminating the need for high temperatures, which could cause RNA degradation. (wikipedia.org)
Analysis1
Clones (672) were screened using PCR to eliminate colonies made up of Cx32 or Cx43, leaving 448 clones that were screened for redundancy by restriction analysis with in situFor Northern blot analysis, the sciatic nerves of anesthetized (50 mg/kg pentobarbital, i.p.), adult (10C13 weeks aged) Sprague Dawley rats were exposed at the sciatic notch. (acp2018.org)
Technique5
Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. (wikipedia.org)
The technique was called the Southern blot. (thedjournal.com)
Burnette took inspiration from the Southern blot technique. (thedjournal.com)
The popularity of the technique spread quickly, and Burnette was soon doing daily seminars over the telephone on his blotting technique. (thedjournal.com)
Harry Towbin and his colleagues at the Friedrich Miescher Institute in Switzerland had created a similar technique that derived inspiration from the northern blot. (thedjournal.com)
Sample1
A general blotting procedure starts with extraction of total RNA from a homogenized tissue sample or from cells. (wikipedia.org)
Group1
The third party shaping Zambia's advent into graphics are the members of the Lusaka Artist Group (as of 1977 renamed Zambia Artists Group or ZAG). (zamart.org)
Eastern1
Today, the naming convention persists, with scientists referring to the eastern blot, southwestern blot and the far-eastern blot, for example, as homages to the work done in the 1970s. (thedjournal.com)
Case1
In the case of the blot, there are also some, directional puns in the naming, showing even scientists have a sense of humor. (thedjournal.com)
History3
Zambia's history of printed art is young and starts just before Independence with the arrival of Cynthia Zukas in what at that time still was Northern Rhodesia. (zamart.org)
In the thirty-year history of the violence, euphemistically termed "the Troubles" in Northern Ireland, it might seem strange that this particular incident should cause such shock and uproar. (encyclopedia.com)
The reasons behind the planting of this bomb are imbedded in the history of the northern Irish state itself, in its constitution, and in the history of its different communities. (encyclopedia.com)
Positive1
A nylon membrane with a positive charge is the most effective for use in northern blotting since the negatively charged nucleic acids have a high affinity for them. (wikipedia.org)
Ireland9
He served as an artillery captain in Northern Ireland but was court-martialed and dismissed after driving a jeep over the fifty-mile limit to visit his Irish fiancée. (theparisreview.org)
Northern Ireland , an area in the north of the island of Ireland that has been part of the British Empire for six hundred years, has experienced horrible religious strife for much of the last century. (encyclopedia.com)
Many Catholics-or republicans-believe Northern Ireland should be independent or should be united with the Republic of Ireland . (encyclopedia.com)
Many Protestants-or unionists/loyalists-believe Northern Ireland should remain part of the United Kingdom. (encyclopedia.com)
Republicans charge gross human rights abuses have been committed by the United Kingdom and by the Protestant army in Northern Ireland. (encyclopedia.com)
Catholics are generally poorer than Protestants in Northern Ireland. (encyclopedia.com)
O n Saturday August 15, 1998, the small town of Omagh in County Tyrone in Northern Ireland became known throughout the world. (encyclopedia.com)
The fact that it was the incident that caused the single greatest number of deaths in Northern Ireland was certainly a factor. (encyclopedia.com)
This policy had long been a corner stone of militant republicanism, which refused to recognize the legality of the Republic of Ireland or of Northern Ireland. (encyclopedia.com)
World1
The international community's shameful acceptance of impunity for Assad and his henchmen is a blot on the conscience of the world. (somalilandsun.com)
Order1
Hepatitis Delta virus (HDV), discovered in 1977, is a defective virus requiring the presence of HBV in order to replicate. (cdc.gov)
Claim1
The Government has repeatedly pledged to downscale the military in the north prior to the Northern Provincial Council elections but residents claim no such move has been made so far. (colombotelegraph.com)
19761
Olive visited Italy and Northern Ireland in 1976. (libcom.org)
Alwine2
The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. (wikipedia.org)
The development of the RNA-based gene expression studies began with the Northern Blot by Alwine et al. (lr-media.nl)
19691
Although sporadic cases of viral encephalitis had been noted in northern Thailand before 1969, epidemic transmission of JE was first recognized that year, when an outbreak leading to 685 cases occurred in the Chiang Mai valley. (cdc.gov)
Commonly3
However, the entire process is commonly referred to as northern blotting. (wikipedia.org)
Commonly cDNA is created with labelled primers for the RNA sequence of interest to act as the probe in the northern blot. (wikipedia.org)
LB is the most commonly reported vector-borne zoonosis in the northern temperate zone, up to 23,000 cases being recorded annually in the United States (US) (4) . (canada.ca)
Cancer3
32. Cancer mortality in four northern wheat-producing states. (nih.gov)
BLOT, W.J., FRAUMENI, J.F., Jr. Geographic patterns of oral cancer in the United States: Etiologic implications. (nih.gov)
Mortality1
Yearly outbreaks numbering in thousands of cases and hundreds of deaths recurred in the northern region and JE became a leading cause of mortality and disability in children. (cdc.gov)
Time1
Saw John Martyn in August 1977 toured New Zealand first time I think for the NZ Student Arts Council with Bert Jansch. (johnmartyn.info)
Western3
Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross-reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)