• 1910
  • In 1910, he was transferred to the Second University Hospital of Vienna, where he devoted himself to developing surgical techniques for the treatment of uterine prolapse. (wikipedia.org)
  • 1950s
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. (wikipedia.org)
  • During the 1950s and the 1960s cell culture was growing in use, increasing production of various containers, media composition and accessories for handling fluids, concentrating cells, isolating organelles, developing staining methods and labels for identifying specific molecules (markers) and more. (celartia.com)
  • agar
  • In 1908, Margaret Reed researched in Berlin in Max Hartmann's lab where she performed probably the first in vitro mammalian cell culture with guinea pig bone marrow by explanting the bone marrow and placing it into a nutrient-rich agar produced by fellow lab researcher Rhoda Erdmann and incubating the sample. (wikipedia.org)
  • Also, he discovered that gonococcus grows best on a culture of agar mixed with human blood serum. (wikipedia.org)
  • In microbiology research, agar - a plant-based goo similar to gelatin and made from seaweed - is extensively used as culture medium. (blogspot.com)
  • Biologist
  • Mikimoto did not know that government biologist Tokichi Nishikawa and a carpenter, Tatsuhei Mise, had each spent time in Australia and learned the secret to spherical pearl production from expatriate British marine biologist William Saville-Kent - inserting a piece of oyster epithelial membrane (the lip of mantle tissue) with a nucleus of shell or metal into an oyster's body or mantle causes the tissue to form a pearl sack. (wikipedia.org)
  • Margaret Adaline Reed Lewis (1881-1970) was an American cell biologist and embryologist who made contributions to cancer research and cell culture techniques, and was likely the first person to successfully grow mammalian tissue in vitro. (wikipedia.org)
  • subsequently
  • Mikimoto Kōkichi (御木本 幸吉, 25 January 1858 - 21 September 1954) was a Japanese entrepreneur who is credited with creating the first cultured pearl and subsequently starting the cultured pearl industry with the establishment of his luxury pearl company Mikimoto. (wikipedia.org)
  • lymph
  • This operation involved removal of the uterus, parametrium, tissues surrounding the upper vagina, and pelvic lymph nodes, but leaving the ovaries intact. (wikipedia.org)
  • biologists
  • As a result, this couple's greatest impact on embryology and cell biology in the twentieth century was teaching later generations of biologists the basic factors involved in tissue culture based on what they had learned from their research. (wikipedia.org)
  • tumors
  • With the exception of some derived from tumors, most primary cell cultures have limited lifespan. (wikipedia.org)
  • In 1907 a bacterium that caused plant tumors, Agrobacterium tumefaciens, had been discovered and in the early 1970s it was found that the bacteria inserted its DNA into plants using a Ti plasmid. (wikipedia.org)
  • grown
  • The tissue grown in this medium was prepared in a method that become known as the "Lewis Culture" where the tissue bits were put into a hanging drop on the underside of a thin glass slip. (wikipedia.org)
  • With so many avenues opened by cell culture to explore, Margaret Lewis and her husband diverged in their area of study, with Margaret Lewis choosing to focus on microbiological problems, which involved close observations of chick embryo intestines reacting to typhoid bacilli in the medium in which it was grown. (wikipedia.org)
  • growth
  • Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (wikipedia.org)
  • Loeffler's best-known work is the elucidation of the characteristics of the diphtheria bacillus and its growth in pure culture (1884), which again clearly revealed the imprint of Koch, with whom he was then so closely associated. (encyclopedia.com)
  • fragments
  • In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vaccinia virus in fragments of guinea pig corneal tissue. (wikipedia.org)
  • reputation
  • He publicly burnt tons of low-quality pearls as a publicity stunt to establish a reputation that the Mikimoto company only sold high-quality cultured pearls. (wikipedia.org)
  • medium
  • However, the major difficulty of the reliable cell culture was not the composition of the medium, it was the contamination, either bacterial, fungal or by yeast. (celartia.com)
  • vaccine
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • By 1898 the culture had been used to develop enough vaccine to allow for the large-scale production of rabies vaccine. (wikipedia.org)
  • method
  • Mikimoto had received a patent in 1896 for producing hemispherical pearls, or mabes, and a 1908 patent for culturing in mantle tissue, but he could not use the Mise-Nishikawa method without invalidating his own patents. (wikipedia.org)
  • Williams developed a new and fast method for preparing and staining brain tissue to show the presence of Negri bodies. (wikipedia.org)
  • methods
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • In 1907, when the American Public Health Association established a committee on the standard methods for the diagnosis of rabies, they named Williams chair of the committee in recognition of her expertise. (wikipedia.org)
  • Several tissue culture methods which measure the rate of chemical absorption by the skin have been approved by the OECD. (wikipedia.org)
  • This technology is an inexpensive and easy-to-use alternative to current 3-D cell culture methods, Napolitano said. (regenerativemedicine.net)
  • That s why several laboratories are pursuing 3-D cell culture methods. (regenerativemedicine.net)
  • There are a few different methods used by the various artists to shape their trees, which share a common heritage with other artistic horticultural and agricultural practices, such as pleaching, bonsai, espalier, and topiary, and employing some similar techniques. (wikipedia.org)
  • These methods use a variety of horticultural and arboricultural techniques to achieve an intended design. (wikipedia.org)
  • development
  • Cell culture turned into basic tool, mainly a routine to produce molecules and a background for testing molecular relations, tumor development and cellular interactions. (celartia.com)
  • biotechnology
  • One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (wikipedia.org)
  • Genetic engineering is the direct manipulation of an organism's genome using certain biotechnology techniques that have only existed since the 1970s. (wikipedia.org)
  • human
  • In 1907, Loeb published a study which showed that breast carcinoma in mice could be hereditary, as it is now known to be in some human cases. (wikipedia.org)