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  • differentiation
  • The interferon-inducible p204 protein acts as a transcriptional coactivator of Cbfa1 and enhances osteoblast differentiation. (jax.org)
  • Knock-out of the DNA-binding activity results in inhibition of osteoblastic differentiation. (wikipedia.org)
  • Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. (abcam.com)
  • Protein ubiquitination and subsequent proteolysis and degradation by the proteasome are important mechanisms in the regulation of the cell cycle, cell growth and differentiation, gene transcription, signal transduction and apoptosis. (wikipedia.org)
  • Recent results suggest that novel CBP-mediated post-translational N-glycosylation activity alters the conformation of CBP-interacting proteins, leading to regulation of gene expression, cell growth and differentiation, Homeodomain interacting protein kinase 2 (HIPK2) phosphorylates several regions of CBP close to the N-terminal and close to the C-terminal region as well. (wikipedia.org)
  • Pathway
  • Analysis of the signal transduction mechanisms underlying the upregulating effect of RA on collagenase-3 expression demonstrated that this factor acts through a signaling pathway involving p38 mitogen-activated protein kinase. (nih.gov)
  • In normoxia, HIF alpha subunits are marked for the ubiquitin-proteasome degradation pathway through hydroxylation of proline-564 and proline-402 by PHD2. (wikipedia.org)
  • β-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. (wikipedia.org)
  • Notably, the C-terminal segment of β-catenin can mimic the effects of the entire Wnt pathway if artificially fused to the DNA binding domain of LEF1 transcription factor. (wikipedia.org)
  • synthesis
  • This effect is dose and time dependent, requires the de novo synthesis of proteins, and is mediated by RAR-RXR heterodimers. (nih.gov)
  • A subcomplex of RPB2 and RPB3 appears soon after subunit synthesis. (wikipedia.org)
  • complex
  • They together participate in the assembly of the transcription preinitiation complex. (wikipedia.org)
  • RNA polymerase holoenzyme complex consisting of core RNA polymerase and a sigma factor executes transcription of a DNA template strand. (wikipedia.org)
  • Once initiation of RNA transcription is complete, the sigma factor can leave the complex. (wikipedia.org)
  • Matrix protein is part of the pre-integration complex. (uniprot.org)
  • Capsid protein p24: Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. (uniprot.org)
  • The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. (wikipedia.org)
  • However, a recent study has shown that σ70 can remain attached in complex with the core RNA polymerase, at least during early elongation. (wikipedia.org)
  • The enzyme has a high affinity for iron(II) and 2-oxoglutarate, and forms a long-lived complex with these factors. (wikipedia.org)
  • This protein is one of the 19 essential subunits of a complete assembled 19S proteasome complex Six 26S proteasome AAA-ATPase subunits (Rpt1, Rpt2, Rpt3, Rpt4, Rpt5, and Rpt6 (this protein)) together with four non-ATPase subunits (Rpn1, Rpn2, Rpn10, and Rpn13) form the base sub complex of 19S regulatory particle for proteasome complex. (wikipedia.org)
  • 26S proteasome complex is usually consisted of a 20S core particle (CP, or 20S proteasome) and one or two 19S regulatory particles (RP, or 19S proteasome) on either one side or both side of the barrel-shaped 20S. (wikipedia.org)
  • To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special degradative tag, the ubiquitinylation. (wikipedia.org)
  • ATPs) in order to facilitate the association between 19S and 20S particles, as well as to cause confirmation changes of alpha subunit C-terminals that form the substate entrance of 20S complex. (wikipedia.org)
  • It is one of ten subunits that complete part of the 10 subunit protein complex (holoTFIIH) and part of a six-subunit complex of Rad3, Tfb1, Tfb2, Tfb4, Tfb5, and Ssl1 (referred to as core) In humans, the function of Tbf5 is clear, as loss of it leads to trichothiosystropy. (wikipedia.org)
  • The TTDA subunit is responsible for the DNA repair function of the complex. (wikipedia.org)
  • Within metazoa (animal kingdom), the core binding factor (CBF)-DNA complex retains a high degree of conservation within the CCAAT binding motif, as well as the sequences flanking this pentameric motif. (wikipedia.org)
  • This region, in particular for the CAAT Box, is located about 60-100 bases upstream (towards the 5' end), however no less than 27 base pairs away, from the initial transcription site or a eukaryote gene in which a complex of general transcription factors bind with RNA Polymerase II prior to the initiation of transcription. (wikipedia.org)
  • The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. (wikipedia.org)
  • The Proteasome and its subunits are of clinical significance for at least two reasons: (1) a compromised complex assembly or a dysfunctional proteasome can be associated with the underlying pathophysiology of specific diseases, and (2) they can be exploited as drug targets for therapeutic interventions. (wikipedia.org)
  • Subsequently, a compromised proteasome complex assembly and function lead to reduced proteolytic activities and the accumulation of damaged or misfolded protein species. (wikipedia.org)
  • β-catenin is regulated and destroyed by the beta-catenin destruction complex, and in particular by the adenomatous polyposis coli (APC) protein, encoded by the tumour-suppressing APC gene. (wikipedia.org)
  • Beta-catenin was initially discovered in the early 1990s as a component of a mammalian cell adhesion complex: a protein responsible for cytoplasmatic anchoring of cadherins. (wikipedia.org)
  • RNA polymerase II (RNAP II and Pol II) is a protein-protein complex. (wikipedia.org)
  • A 550 kDa complex of 12 subunits, RNAP II is the most studied type of RNA polymerase. (wikipedia.org)
  • Once RPB1 enters the complex, other subunits such as RPB5 and RPB7 can enter, where RPB5 binds to RPB6 and RPB8 and RPB3 brings in RPB10, RPB 11, and RPB12. (wikipedia.org)
  • Transcription factor II B (TFIIB) is a general transcription factor that is involved in the formation of the RNA polymerase II preinitiation complex (PIC) and aids in stimulating transcription initiation. (wikipedia.org)
  • TFIIB is localised to the nucleus and provides a platform for PIC formation by binding and stabilising the DNA-TBP (TATA-binding protein) complex and by recruiting RNA polymerase II and other transcription factors. (wikipedia.org)
  • The protease resistant C terminal core stabilises the DNA-TBP complex by interacting with nonspecific sequences either side of the TATA box called the upstream and downstream B recognition elements (BREu and BREd), as well as interacting with the Initiator element (INR). (wikipedia.org)
  • The binding of TFIIB up and downstream of the TATA box strengthens this complex but this binding is not sequence specific as the TFIIB does not come into contact with any of the DNA bases. (wikipedia.org)
  • In 1954, Pauling was awarded his first Nobel Prize "for his research into the nature of the chemical bond and its application to the elucidation of the structure of complex substances" (such as proteins), prominently including the structure of the α-helix. (wikipedia.org)
  • regulation
  • Regulation of adult bone mass by the zinc finger adapter protein Schnurri-3. (jax.org)
  • In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. (wikipedia.org)
  • β-catenin is a dual function protein, involved in regulation and coordination of cell-cell adhesion and gene transcription. (wikipedia.org)
  • Not only their ARM domains resemble each other in both architecture and ligand binding capacity, but the N-terminal β-TrCP-binding motif is also conserved in plakoglobin, implying common ancestry and shared regulation with β-catenin. (wikipedia.org)
  • structural
  • In addition, to gain structural and quantitative insight, we investigated the binding of Securin to p53. (jove.com)
  • These proteins have a structural motif consisting of a 2-layer sandwich structure with an alpha/beta plait topology. (wikipedia.org)
  • nucleotides
  • It also have subunits that can bind with nucleotides (e.g. (wikipedia.org)
  • In molecular biology, a CCAAT box (also sometimes abbreviated a CAAT box or CAT box) is a distinct pattern of nucleotides with GGCCAATCT consensus sequence that occur upstream by 60-100 bases to the initial transcription site. (wikipedia.org)
  • This same experiment also yielded the same sequence as shown above when using a different oligonucleotide (R2) that contained an ATTGG core and flanked by 12 5' random nucleotides and 10 3' random nucleotides. (wikipedia.org)
  • Sequence
  • The consensus binding site for CBF has been identified to be a 7 bp sequence PyGPyGGTPy. (wikipedia.org)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • The CAAT box signals the binding site for the RNA transcription factor, and is typically accompanied by a conserved consensus sequence. (wikipedia.org)
  • By using PCR-mediated random binding selection process, researchers were able to show that the sequence "3' - (T/C)G ATTGG (T/C)(T/C)(A/G) - 5'" immediately flanking the ATTGG region (CCAAT in the complementary strand) was preferentially selected on the coding strand (opposite of the template strand). (wikipedia.org)
  • The CREB protein domains, KIX, TAZ1 and TAZ2, each bind tightly to a sequence spanning both transactivation domains 9aaTADs of transcription factor p53. (wikipedia.org)
  • This protein shares regions of very high-sequence similarity with protein EP300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. (wikipedia.org)
  • The Alpha Helix (α-helix) is a common motif in the secondary structure of proteins and is a righthand-spiral conformation (i.e. helix) in which every backbone N−H group donates a hydrogen bond to the backbone C=O group of the amino acid located three or four residues earlier along the protein sequence. (wikipedia.org)
  • Among types of local structure in proteins, the α-helix is the most regular and the most predictable from sequence, as well as the most prevalent. (wikipedia.org)
  • helices
  • The conserved region contains four alpha helices and three loops arranged as in histone H3. (wikipedia.org)
  • The catalytic domain consists of a double-stranded β-helix core that is stabilized by three α-helices packed along the major β-sheet. (wikipedia.org)
  • Structurally, it contains a 33-amino acid ankyrin repeat that forms a series of alpha helices. (wikipedia.org)
  • The first repeat of β-catenin (near the N-terminus) is slightly different from the others - as it has an elongated helix with a kink, formed by the fusion of helices 1 and 2. (wikipedia.org)
  • Each of the domains has 5 alpha helices with a hydrophobic core. (wikipedia.org)
  • The α-helices can be identified in protein structure using several computational methods, one of which being DSSP (Dictionary of Protein Secondary Structure). (wikipedia.org)
  • molecular
  • Sigma factors are distinguished by their characteristic molecular weights. (wikipedia.org)
  • For example, σ 70 is the sigma factor with a molecular weight of 70 kDa . (wikipedia.org)
  • Fluorescens Anisotropi som værktøj til at studere protein-protein interaktioner Abril Gijsbers 1 , Takuya Nishigaki 2 , Nuria Sánchez-Puig 1 1 Departamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, 2 Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México Protein interaktioner er kernen i en celles funktion. (jove.com)
  • RPB3
  • RPB3 (POLR2C) - the third-largest subunit. (wikipedia.org)
  • RPB3, RPB5, and RPB7 interact with themselves to form homodimers, and RPB3 and RPB5 together are able to contact all of the other RPB subunits, except RPB9. (wikipedia.org)
  • domain
  • Contains 1 Runt domain. (abcam.com)
  • Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2), or prolyl hydroxylase domain-containing protein 2 (PHD2), is an enzyme encoded by the EGLN1 gene. (wikipedia.org)
  • 9aaTAD is a novel domain common to a large superfamily of eukaryotic transcription factors represented by Gal4, Oaf1, Leu3, Rtg3, Pho4, Gln4, Gcn4 in yeast and by p53, NFAT, NF-κB and VP16 in mammals. (wikipedia.org)
  • The Runt domain is an evolutionary conserved protein domain. (wikipedia.org)
  • This domain occurs towards the N-terminus of the proteins in this entry. (wikipedia.org)
  • A small molecule inhibitor (I-CBP112) binding to the bromodomain domain of CBP/p300 has been developed for leukaemia therapy. (wikipedia.org)
  • Termed armadillo repeats, all these elements fold together into a single, rigid protein domain with an elongated shape - called armadillo (ARM) domain. (wikipedia.org)
  • This segment is not fully disordered: part of the C-terminal extension forms a stable helix that packs against the ARM domain, but may also engage separate binding partners. (wikipedia.org)
  • As sketched above, the ARM domain of beta-catenin acts as a platform to which specific linear motifs may bind. (wikipedia.org)
  • Located in structurally diverse partners, the β-catenin binding motifs are typically disordered on their own, and typically adopt a rigid structure upon ARM domain engagement - as seen for short linear motifs. (wikipedia.org)
  • In combination with several other polymerase subunits, the RPB1 subunit forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. (wikipedia.org)
  • The core domain consists of two alpha helical structures that form nearly identical domains connected by a short linker region and rotated by 90 degrees between each other. (wikipedia.org)
  • degradation
  • These proteolytic active sites located in the inner side of a chamber formed by 4 stacked rings of 20S subunits, preventing random protein-enzyme encounter and uncontrolled protein degradation. (wikipedia.org)
  • The 19S regulatory particles can recognize ubiquitin-labeled protein as degradation substrate, unfold the protein to linear, open the gate of 20S core particle, and guide the substate into the proteolytic chamber. (wikipedia.org)
  • It also binds closely to the E3 ubiquitin ligase MDM2, which is a regulator of the degradation of p53 and retinoblastoma protein, both transcription factors involved in tumor suppression and found mutated in many cancers. (wikipedia.org)