• guanine-nucleo
  • Dysfunction of alsin, particularly its putative Rab5 guanine-nucleotide-exchange factor activity, has been linked to one form of juvenile onset recessive familial amyotrophic lateral sclerosis (ALS2). (whiterose.ac.uk)
  • vesicle
  • Confocal colocalization studies between apical and basal vesicular markers and the different PCDH15 and VLGR1 variants along with sucrose density gradients and the use of vesicle trafficking inhibitors show the existence of Usher protein complexes in at least two vesicular subpools. (biomedsearch.com)
  • The apically trafficked pool colocalized with the early endosomal vesicle marker, rab5, while the basally trafficked pool associated with membrane microdomains and SNAP25. (biomedsearch.com)
  • Collectively, these findings establish the existence of a differential vesicular trafficking mechanism for specific Usher protein variants in mouse cochlear hair cells, with the apical variants playing a potential role in endosomal recycling and stereocilia development/maintenance, and the basolateral variants involved in vesicle docking and/or fusion through SNAP25-mediated interactions. (biomedsearch.com)
  • 2004). To determine whether the interaction between Na/K-ATPase and Cav1 plays a role in regulating caveolar vesicle trafficking, we first performed fluorescence resonance energy transfer (FRET) analysis to test whether these two proteins have the potential to directly interact in live cells. (nih.gov)
  • The available data concerning the involvement of these mG-proteins in the control of vesicle trafficking agree generally with the paradigms accepted for other eukaryotes. (deepdyve.com)
  • Protein trafficking in the secretory pathway requires a series of events including cargo selection and vesicle budding at the donor organelle, followed by transport, docking, and fusion of transport vesicles with the appropriate target organelle. (rupress.org)
  • In this Cell Science at a Glance and the accompanying poster, we highlight the regulation of Rab GTPases by proteins that control their membrane association and activation state, and provide an overview of the cellular processes that are regulated by Rab GTPases and their effectors, including protein sorting, vesicle motility and vesicle tethering. (biologists.org)
  • RAB proteins are small GTPases involved in exocytic and endocytic pathways of eukaryotic cells, controlling vesicle docking and fusion. (unifesp.br)
  • Hereafter, the activated form of Rab, GTP-bound, is incorporated to a specific organelle or vesicle and promotes its transport by interacting with a specific effector molecule. (wikipedia.org)
  • The process of endocytosis works by folding the cell membrane around a substance outside of the cell (for example a protein) and then forms a vesicle. (wikipedia.org)
  • endosomal
  • Excessive accumulation of Rab5-positive vesicles was observed in ALS2(-/-) neurons, which correlated with a significant reduction in endosomal motility and augmentation in endosomal conversion to lysosomes. (whiterose.ac.uk)
  • These phenotypes closely resembled the endosomal trafficking abnormalities induced by a constitutively active form of Rab5 in wild-type neurons. (whiterose.ac.uk)
  • Therefore, our findings reveal a negatively regulatory mechanism of alsin in Rab5-mediated endosomal trafficking, suggesting that enhanced endosomal degradation in ALS2(-/-) neurons may underlie the pathogenesis of motor neuron degeneration in ALS2 and related motor neuron diseases. (whiterose.ac.uk)
  • Although the SNX dimer is required for the recruitment of retromer to the endosomal membrane, the cargo binding function of this complex is contributed by the core trimer through the binding of Vps35 subunit to various cargo molecules including M6PR wntless and sortilin. (wikipedia.org)
  • The SNX-BAR dimer enters the nucleation complex via direct binding or lateral movement on endosomal surface. (wikipedia.org)
  • genes
  • Mouse models carrying mutations for the nine Usher-associated genes have splayed stereocilia, and some show delayed maturation of ribbon synapses suggesting these proteins may play different roles in terminal differentiation of auditory hair cells. (biomedsearch.com)
  • In this study we have examined the functions for four rab5 genes in zebrafish. (zfin.org)
  • By contrast MO disruption of other zebrafish rab5 genes shows little or no effect on expression of markers of dorsal organiser development. (zfin.org)
  • effectors
  • Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest. (nih.gov)
  • Microinjection into macrophages of antibodies against EEA1 and the PI-3K hVPS34 reduced acquisition of late endocytic markers by latex bead phagosomes, demonstrating an essential role of these Rab5 effectors in phagosomal biogenesis. (nih.gov)
  • intracellular
  • Finally, analysis of the defects in cellular PtdIns(3)P levels in various vps15 and vsp34 mutant strains has led to additional insights into the importance of PtdIns(3)P intracellular localization, as well as the roles of Vps15p and Vps34p in vacuolar protein sorting. (pubmedcentralcanada.ca)
  • As distinct from animal cells comprising 40 subfamilies of Rab proteins, which are the key regulators of intracellular vesicular transport, numerous Rab proteins in Arabidopsis and other plant species could be grouped in only eight subfamilies on the basis of their functional properties. (deepdyve.com)
  • I n eukaryotic cells, the functional properties of a particular intracellular organelle are largely defined by its unique set of resident proteins. (rupress.org)
  • This is a sub-part (intracellular signaling peptides and proteins only) of List of MeSH codes (D12.776), itself a part of the list of the "D" codes for MeSH. (wikipedia.org)
  • Members of the RAB family of RAS-related GTP-binding proteins are important regulators of vesicular transport and are located in specific intracellular compartments. (wikipedia.org)
  • nucleotide
  • Both compounds inhibited the xanthosine-diphosphate-dependent prenylation of a mutant of Rab5, Rab5D136N, the nucleotide specificity of which had been converted from GTP to xanthosine triphosphate. (umassmed.edu)
  • molecules
  • Fantl WJ, Escobedo JA, Martin GA, Turck CW, del Rosario M, McCormick F, Williams LT. Distinct phosphotyrosines on a growth factor receptor bind to specific molecules that mediate different signaling pathways. (pubmedcentralcanada.ca)
  • VAM3 encodes a 283-amino acid protein that shares homology with the syntaxin family of t-SNARE molecules. (rupress.org)
  • Surface protein recycling returns proteins to the surface whose function involves carrying another protein or substance inside the cell, such as the transferrin receptor, or serves as a means of regulating the number of a certain type of protein molecules on the surface. (wikipedia.org)
  • vacuolar protein
  • This rapid block in vacuolar protein sorting appears to be the result of loss of PtdIns 3- kinase activity since cellular PtdIns(3)P levels decrease dramatically in vps34 temperature-sensitive mutant cells that have been incubated at the nonpermissive temperature. (pubmedcentralcanada.ca)
  • Retromer is a heteropentameric complex which in humans is composed of a less defined membrane-associated sorting nexin dimer (SNX1, SNX2, SNX5, SNX6), and a vacuolar protein sorting (Vps) trimer containing Vps26, Vps29, Vps35. (wikipedia.org)
  • plasma membrane
  • When the plasma membrane signals were analyzed, we found significant FRET between this pair of proteins (Fig. 4, A and B), indicating that the Na/K-ATPase and Cav1 are likely to interact in the plasma membrane. (nih.gov)
  • To function and transmit signals, β1 integrins must localize to the plasma membrane and be able to relocate to focal adhesions upon ligand binding. (biologists.org)
  • These processes make up the route through which cell surface proteins are trafficked from the Golgi to the plasma membrane and are recycled. (wikipedia.org)
  • yeast
  • A membrane-associated complex composed of the Vps15 protein kinase and the Vps34 phosphatidylinositol 3-kinase (PtdIns 3-kinase) is essential for the delivery of proteins to the yeast vacuole. (pubmedcentralcanada.ca)
  • Isolation of yeast mutants defective in protein targeting to the vacuole. (pubmedcentralcanada.ca)
  • Protein transport to the vacuole in the budding yeast Saccharomyces cerevisiae is one system in which these processes have been extensively studied. (rupress.org)
  • RAB7a was cloned by screening a human placenta cDNA library with a rat Rab7 cDNA to show that the RAB7a cDNA encodes a 207-amino acid protein whose sequence is 99% identical to those of mouse, rat, and dog Rab7a and 61% identical to that of yeast Rab7a. (wikipedia.org)
  • delivery of proteins
  • These data indicate that an intact Vps15p protein kinase domain is necessary for the association with and activation of Vps34p, and they demonstrate that a functional Vps15p-Vps34p complex is absolutely required for the efficient delivery of proteins to the vacuole. (pubmedcentralcanada.ca)
  • Maintenance of organelle identity therefore requires effective sorting and delivery of proteins. (rupress.org)
  • membranes
  • Polyclonal antiserum raised against Vam3p recognized a 35-kD protein that was associated with vacuolar membranes by subcellular fractionation. (rupress.org)
  • kinase
  • Consistent with this, mutations altering highly conserved residues in the lipid kinase domain of Vps34p lead to a dominant-negative phenotype resulting from titration of activating Vps15 proteins. (pubmedcentralcanada.ca)
  • Carlberg K, Tapley P, Haystead C, Rohrschneider L. The role of kinase activity and the kinase insert region in ligand-induced internalization and degradation of the c-fms protein. (pubmedcentralcanada.ca)
  • Phosphoinositide 3-kinase is activated by phosphopeptides that bind to the SH2 domains of the 85-kDa subunit. (pubmedcentralcanada.ca)
  • Ligand and protein kinase C downmodulate the colony-stimulating factor 1 receptor by independent mechanisms. (pubmedcentralcanada.ca)
  • 2002
  • Upon ligand binding, heterodimeric α/β integrin receptors cluster to cytoskeleton-linked focal adhesion complexes and induce the recruitment of kinases and adaptor proteins leading to the transduction of signals to the cell interior (reviewed by Hynes, 2002 ). (biologists.org)
  • interactions
  • These studies reveal a complex network of interactions between the proteins required for endosome fusion. (ox.ac.uk)
  • Genetic interactions between VAM3 and a SEC1 family member, VPS33 , suggest the two proteins may act together to direct the docking and/or fusion of multiple transport intermediates with the vacuole. (rupress.org)
  • Other key interactions include RAC1 (By similarity), NTRK1/TRKA (By similarity), C9orf72 (By similarity), CHM (the substrate-binding subunit of the Rab geranylgeranyltransferase complex), and RILP, as well as PSMA7, RNF115 and FYCO1. (wikipedia.org)
  • Rab7
  • In growth factor-deprived cells, however, blocking Rab7 function prevented the clearance of glucose and amino acid transporter proteins from the cell surface. (wikipedia.org)
  • The authors concluded that RAB7 functions as a proapoptotic protein by limiting cell-autonomous nutrient uptake. (wikipedia.org)
  • Golgi
  • Retromer plays a central role in the retrieval of several different cargo proteins from the endosome to the trans-Golgi network. (wikipedia.org)
  • EEA1
  • We report the requirement for 3-phosphoinositides and acquisition of Rab5 effector early endosome autoantigen (EEA1) as essential molecular events necessary for phagosomal maturation. (nih.gov)
  • To test this hypothesis, we generated truncated (amino acids 1277--1411) and full-length EEA1 constructs containing point mutations in the COOH terminus that impair Rab5 but not PtdIns-3-P binding. (umassmed.edu)
  • These results suggest that association with Rab5 is not necessary for the initial binding and tethering functions of EEA1. (umassmed.edu)
  • Relationships between EEA1 binding partners and their role in endosome fusion. (ox.ac.uk)
  • In the case of EEA1, binding to calmodulin requires an IQ domain, which is adjacent to a C-terminal FYVE domain that specifically binds to PI3P. (ox.ac.uk)
  • vesicular
  • the identification of proteins involved in T. cruzi endo- and exocytic pathways may generate cellular compartment markers, an invaluable tool to better understand the vesicular transport in this parasite. (unifesp.br)
  • molecular
  • so they can be used as molecular markers for studying protein trafficking in Trypanosoma cruzi, the causal agent of Chagas' disease. (unifesp.br)
  • biotic
  • On the other hand, these proteins play an important role in plant responses to abiotic and biotic factors, indicating specific for plants functions of Rab proteins. (deepdyve.com)
  • terminus
  • We have shown that the Na/K-ATPase can be coimmunoprecipitated with Cav1 and that the purified Na/K-ATPase binds to GST-Cav1 N terminus (Wang et al. (nih.gov)