OocytesTropicalisOocyteMRNAEmbryosMutationTranscriptional ActivationGenesCDNATransmembraneReceptorsBindsMammalianExpressionReceptor ProteinJunction proteinsHeat shock pMolecularActivationHistoneOctamer-bindingMetamorphosisThyroid1998BINDING PROTEINThiolModulateSubstancesThesisSerum1987FrogDevelopment1996EnzymeChromatinResearchRegulation
Oocytes17
- Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. (biologists.com)
- Mature Xenopus oocytes have diameters of 1 to 1.2mm. (researchbib.com)
- Xenopus oocytes have been extensively used to study biogenesis, functional architecture, and modulation of plasma membrane expression of mRNAs encoding membrane receptors or ion channels. (researchbib.com)
- reported that Xenopus oocytes translate foreign mRNA. (researchbib.com)
- Xenopus oocytes are capable of translation and post-translational modification (Ghysdael et al. (researchbib.com)
- 1982) presented the first electrophysiological evidence for the expression of functional nAChR in Xenopus oocytes. (researchbib.com)
- 1987) reported for the first time that Xenopus oocytes successfully express glutamate receptors from lobster (Palinurus japonicus ) abdominal muscle. (researchbib.com)
- 1991) recently reported that C. elegans poly A+ RNA injected into Xenopus oocytes expresses L-glutamate-sensitive chloride channels. (researchbib.com)
- Thesaurin a , the major protein of Xenopus laevis previtellogenic oocytes, present in the 42 S particles, is homologous to elongation factor EF-1 alpha . (xenbase.org)
- We suggest that all three proteins involved in 5 S RNA and tRNA storage in previtellogenic oocytes, TFIIIA , thesaurin a and thesaurin b , have a dual function: storage and a role in transcription or in protein synthesis. (xenbase.org)
- The increase in Mos protein and the activation of MAPK by injecting cyclin A protein into immature oocytes were both blocked also by cycloheximide treatment. (elsevierpure.com)
- To investigate the mechanism of the egl-2(gf) phenotype and rescue by imipramine, we analyzed EGL-2 currents in Xenopus oocytes. (jneurosci.org)
- Studies using Xenopus cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes-from mechanisms underlying the cell cycle to embryonic development to human disease. (cshlpress.com)
- Influenza D virus M2 protein exhibits ion channel activity in Xenopus laevis oocytes. (cdc.gov)
- These snoRNAs were used in cross-linking studies to identify which proteins assembled on them in vivo in Xenopus oocytes. (fishersci.com)
- These OsVPE proteins can transport Pi into yeast cells and Xenopus laevis oocytes. (bvsalud.org)
- Expression of the mutated genes in xenopus oocytes has provided some insight into how the potassium channel mutation leads to lowering of the seizure threshold. (medscape.com)
Tropicalis4
- It shared 94% identity with X. tropicalis HSPB6 but only 49% and 59% with zebrafish and human proteins, respectively. (uwaterloo.ca)
- The manual covers both Xenopus laevis and the smaller Xenopus tropicalis species. (cshlpress.com)
- Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis. (xenbase.org)
- Xenopus tropicalis DnaJ heat shock protein family (Hsp40) member B11 (dnajb11), mRNA. (genscript.com)
Oocyte11
- The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. (biologists.com)
- 1986). A Xenopus oocyte can also assemble the subunits of a receptor and insert them into its surface membrane (Parker et al. (researchbib.com)
- 1990) published that amino acid receptors expressed in Xenopus oocyte after " microinjection of mRNA from the leg of locust Schistocerca gregaria. (researchbib.com)
- RNA derived from alpha-L1 cDNA when injected alone in Xenopus oocyte resulted in the expression of functional nAChR. (researchbib.com)
- Two octamer-binding proteins have been identified (in band shift assays) in X. laevis oocyte, embryo and tissue extract. (warwick.ac.uk)
- Histone protein and message are stored in the oocyte as part of the mechanism to provide enough histones to keep-up with the high rate of DNA synthesis in early Xenopus development. (warwick.ac.uk)
- A polyclonal antiserum raised against the N terminal domain of X. laevis Oct-1 reacts to proteins other than Oct-1 on Western blots of oocyte and embryo extract. (warwick.ac.uk)
- These proteins, which are antigenically related to the N terminal domain of Oct-1, are entirely located in the cytoplasm of the oocyte, and entirely located in the nucleus of somatic cells. (warwick.ac.uk)
- These proteins are synthesised during oogenesis, and stored in the oocyte in an amount equivalent to at least 100 000 somatic cells. (warwick.ac.uk)
- Mitogen-activated protein kinase (MAPK) and MAPK kinase (MAPKK) are activated during Xenopus oocyte maturation concomitant with the activation of maturation promoting factor (MPF). (elsevierpure.com)
- Injection of recombinant STE11 protein (a yeast MAPKK kinase) also induced initiation of oocyte maturation. (elsevierpure.com)
MRNA2
- Interestingly, injection of the active MAPKK mRNA or the STE11 protein resulted in induction and accumulation of Mos protein. (elsevierpure.com)
- Expression of mouse zonadhesin mRNA is evident only within the testis, and the protein is found exclusively on the apical region of the sperm head. (embl.de)
Embryos6
- In Xenopus embryos, XMeis3 protein activity is required for normal hindbrain formation. (xenbase.org)
- HoxD1 is an XMeis3 direct-target gene, and ectopic HoxD1 expression rescues cell fate losses in either XMeis3 or Zic protein knock down embryos. (xenbase.org)
- FGF3 and FGF8 are direct target genes of XMeis3 protein and their expression is lost in XMeis3 morphant embryos. (xenbase.org)
- A) Two-cell stage albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 100 pg of RNA encoding the HoxD1 antimorph protein. (xenbase.org)
- 1/Ventx2.1 in Early Xenopus Embryos. (xenbase.org)
- PP1 expression synergistically activates, and inhibition of PP1 inhibits, Wnt/beta-catenin signaling in Drosophila and mammalian cells as well as in Xenopus embryos. (duke.edu)
Mutation2
- Jul 2009;1(1):a000802 and is reprinted with permission of Cold Spring Harbor Press](B) lrd (left-right dynein), the protein (green) mutated by the iv mutation, is also known as DNAH11, DNAHC11, and DLP11. (medscape.com)
- When the iv mouse mutation was cloned, it was found to encode a molecular motor protein, an axonemal dynein, and was named lrd , for left-right dynein (human homolog is DNAH11 / DNAHC11 ). (medscape.com)
Transcriptional Activation1
- Both DP-1 and DP-2 synergistically interact with members of the E2F family of proteins, E2F-1, E2F-2, and E2F-3, to generate DNA binding complexes that specifically recognize the E2F site and functionally interact with E2F-1 in E2F site-dependent transcriptional activation of cellular genes. (ox.ac.uk)
Genes3
- Our results show that XMeis3 protein knock down also causes a loss of primary neuron and neural crest cell lineages, without altering expression of Zic, Sox or Pax3 genes. (xenbase.org)
- The valuable source of large-scale genomic information initiated attempts to identify the origin(s) of G protein-coupled receptors (GPCR), count and categorize those genes, and follow their evolutionary history. (springer.com)
- The Popeye domain containing (POPDC) genes encode sarcolemma-localized cAMP effector proteins. (imperial.ac.uk)
CDNA2
- Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (heat-shock.com)
- These sequences represent the protein coding region of the dnajb11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
Transmembrane3
- Transmembrane H+ fluxes and the regulation of neural induction in Xenopus laevis. (xenbase.org)
- FHM type 4 is attributed to mutations in the PRRT2 gene, which encodes a proline-rich transmembrane protein of as yet unknown function. (frontiersin.org)
- which encodes a proline-rich transmembrane protein of still unknown function. (frontiersin.org)
Receptors4
- Schioth HB, Fredriksson R (2005) The GRAFS classification system of G-protein coupled receptors in comparative perspective. (springer.com)
- Sequence comparison does not support an evolutionary link between halobacterial retinal proteins including bacteriorhodopsin and eukaryotic G-protein-coupled receptors. (springer.com)
- Apical endosomal glycoprotein from rat, a protein probably involved in the sorting and selective transport of receptors and ligands across polarized epithelia. (embl.de)
- We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)
Binds4
- Here, we have defined DRTF1/E2F in Xenopus laevis that, like its mammalian counterpart, specifically binds to the E2F site, is regulated during development, and interacts with pRb and related proteins. (ox.ac.uk)
- Group A Streptococcus (GAS), a β-haemolytic human pathogen, expresses a NEAT protein, Shr, which binds several haemoproteins and extracellular matrix (ECM) components. (tcdb.org)
- Pig zonadhesin, a protein that binds in a species-specific manner to the zona pellucida of the egg. (embl.de)
- A pig sperm protein that binds to the extracellular matrix of the egg in a species-specific manner was recently identified and named zonadhesin (Hardy, D. M., and Garbers, D. L. (1995) J. Biol. (embl.de)
Mammalian3
- HSPB6 was determined to be an ortholog of mammalian HSPB6 and encoded a 168 amino acid protein that contained an α-crystallin domain, a polar C-terminal extension and possible phosphorylation sites. (uwaterloo.ca)
- It is widely believed that in mammalian cells the cellular transcription factor (DRTF1/E2F integrates cell-cycle events with the transcription apparatus by interacting with important regulators of the cell cycle, such as the retinoblastoma gene product (pRb) and related proteins, cyclins, and cyclin-dependent kinases. (ox.ac.uk)
- There have been three types of gap junctional proteins (connexins) identified in the mammalian lens: Cx43, Cx46, Cx50. (rosalindfranklin.edu)
Expression8
- Knock down or inhibition of the Pax3 , Zic1 or Zic5 protein activities extinguishes embryonic expression of the XMeis3 gene, as well as triggering the loss of hindbrain , neural crest and primary neuron cell fates. (xenbase.org)
- Ectopic expression of the HoxD1 antimorph protein knockdown inhibits primary neuron and neural crest markers in addition to hindbrain markers. (xenbase.org)
- The purpose of heterologous expression is often to determine the effects of mutations and differential interactions on protein function. (wikipedia.org)
- green fluorescent protein (GFP) fusions revealed egl-2 expression in a subset of neurons and muscles that could explain the mutant behavioral defects. (jneurosci.org)
- GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (genscript.com)
- Protein expression is influenced by many factors that may vary between experiments or laboratories. (genscript.com)
- Scope includes mutations and abnormal protein expression. (cancerindex.org)
- Sequential expression of multiple POU proteins during amphibian early development. (wikigenes.org)
Receptor Protein1
- MAM is an acronym derived from meprin, A-5 protein, and receptor protein-tyrosine phosphatase mu. (embl.de)
Junction proteins1
- It has been recently shown that mutations of lens gap junction proteins are associated with certain forms of inherited congenital cataract. (rosalindfranklin.edu)
Heat shock p1
- The final chapter of this thesis investigated characteristics of the Xenopus small heat shock protein HSPB6, a HSP whose levels were not affected by ITC treatment of A6 cells. (uwaterloo.ca)
Molecular4
- As electrophiles, the impact of ITCs at the molecular level is profound, with the ability to directly bind and modify thiol-containing compounds such as glutathione and cellular protein, including tubulin. (uwaterloo.ca)
- Since a great deal of the molecular and cellular research with Xenopus is applicable to humans, an increase in our knowledge of the effects of ITCs is of importance given their potential role as therapeutic compounds in the treatment of cancer, neurodegenerative diseases and bacterial and viral infections. (uwaterloo.ca)
- Molecular characterization of Xenopus laevis DP proteins. (ox.ac.uk)
- One of the major aims of Dr. Ebihara's research project are to obtain a more detailed understanding of the biophysical properties of cloned lens gap junctional proteins and to localize the molecular determinants of permeability and gating of the gap junctional channels. (rosalindfranklin.edu)
Activation2
- The canonical TGFβ signalling pathway involves ligand-dependent assembly of a heteromeric receptor complex, receptor-kinase activation and subsequent phosphorylation and activation of SMAD proteins, which are transcriptional regulators that consequently accumulate in the nucleus. (nature.com)
- The 96 kDa protein kinase activated by oncogenic Ras in Xenopus egg extracts is also activated by constitutively active Mek: activation requires serine/threonine phosphorylation. (wikigenes.org)
Histone1
- Histone H2B gene transcription during Xenopus early development requires functional cooperation between proteins bound to the CCAAT and octamer motifs. (wikigenes.org)
Octamer-binding1
- Oct-1, and a second, previously unidentified octamer-binding protein which has been termed Oct-R, for octamer-related. (warwick.ac.uk)
Metamorphosis2
- We analyzed genome-wide changes in DNA methylation and their relationship to gene regulation in the brain of Xenopus tadpoles during metamorphosis, a thyroid hormone-dependent developmental process. (nih.gov)
- There are protocols for analyses of Xenopus immunology, neurobiology, metamorphosis, and adult physiology. (cshlpress.com)
Thyroid1
- Xenopus laevis thyroid hormone induced protein B. This protein contains 4 MAM domains. (embl.de)
19981
- Oesterhelt D (1998) The structure and mechanism of the family of retinal proteins from halophilic archaea. (springer.com)
BINDING PROTEIN1
- Matrix Gla protein (MGP) is an extracellular mineral-binding protein expressed in several tissues but it only accumulates in bone and calcified cartilage under physiological conditions. (bath.ac.uk)
Thiol1
- While the biochemical effects of ITCs have been well characterized, less information is available regarding their effects on the accumulation of stress-inducible heme oxygenase-1 (HO-1) and heat shock proteins (HSPs) as well as the possible formation of aggregated protein due to thiol modification. (uwaterloo.ca)
Modulate1
- Previous studies suggest that the vesicular cysteine-string protein (CSP) may modulate presynaptic Ca 2+ channel activity in fast neurotransmitter release. (jneurosci.org)
Substances1
- Transport proteins that carry specific substances in the blood or across cell membranes. (reference.md)
Thesis1
- The research detailed in this thesis examined the effect of BITC, PEITC and SFN on the accumulation of HO-1 and HSPs and documented the impact of BITC and PEITC on aggregated protein in a kidney epithelial cell line. (uwaterloo.ca)
Serum1
- cDNAs of Guinea pigs are also very popular.Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. (heat-shock.com)
19871
- 1987), Thesaurin a, the major protein of Xenopus laevi. (xenbase.org)
Frog2
- In the present study, I examined the effect of benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PEITC), and sulforaphane (SFN) on the accumulation of HO-1, HSP70, HSP30 and HSPB6 in A6 kidney epithelial cells derived from the frog, Xenopus laevis. (uwaterloo.ca)
- Members of the frog Xenopus genus are versatile model organisms used in developmental biology, cell biology, neuroscience, and toxicology research, among others. (cshlpress.com)
Development4
- Xenopus Meis3 protein lies at a nexus downstream to Zic1 and Pax3 proteins, regulating multiple cell-fates during early nervous system development. (xenbase.org)
- Thus, XMeis3 protein is positioned at a key regulatory point, simultaneously regulating multiple neural cell fates during early vertebrate nervous system development. (xenbase.org)
- Normal Table of Xenopus development: a new graphical resource. (xenbase.org)
- Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. (cancerindex.org)
19961
- Kim JY, Haastert PV, Devreotes PN (1996) Social senses: G-protein-coupled receptor signaling pathways in Dictyostelium discoideum . (springer.com)
Enzyme1
- Then, a survey is given about ATP1A2 mutations implicated in migraine cases as documented in the literature with focus on mutations that were described to completely destroy enzyme function, or lead to misfolded or mistargeted protein in particular model cell lines. (frontiersin.org)
Chromatin1
- Histones are the main protein components of chromatin. (histoready.com)
Research4
- This new laboratory manual provides a comprehensive collection of experimental procedures for research using Xenopus . (cshlpress.com)
- It is a critical reference for laboratories that currently work with these organisms and will serve as an essential start-up guide for those seeking to start using Xenopus frogs in their research for the first time. (cshlpress.com)
- Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative. (genscript.com)
- One of the current areas of interest in the field of eye research is to understand how these gap junctional proteins contribute to lens homeostasis and how cataracts arise. (rosalindfranklin.edu)
Regulation1
- Constam, D. B. & Robertson, E. J. Regulation of bone morphogenetic protein activity by pro domains and proprotein convertases. (nature.com)