Furthermore, Xenopus oocytes are a leading system for studies of ion transport and channel physiology. (wikipedia.org)
Studies using Xenopus cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes-from mechanisms underlying the cell cycle to embryonic development to human disease. (cshlpress.com)
Microinjection of the major cluster clones in to Xenopus oocytes, confirmed that these genes were functional. (bl.uk)
Effects of steroids on gamma-aminobutyric acid receptors expressed in Xenopus oocytes by poly(A)+ RNA from mammalian brain and retina. (aspetjournals.org)
Electrical recordings were made in Xenopus oocytes to study the modulatory effects of steroids on gamma-aminobutyric acid (GABA) receptors expressed by RNA from mammalian brain and retina. (aspetjournals.org)
Influenza D virus M2 protein exhibits ion channel activity in Xenopus laevis oocytes. (cdc.gov)
Tropicalis7
The two best-known species of this genus are Xenopus laevis and Xenopus tropicalis, which are commonly studied as model organisms for developmental biology, cell biology, toxicology, neuroscience and for modelling human disease and birth defects. (wikipedia.org)
The manual covers both Xenopus laevis and the smaller Xenopus tropicalis species. (cshlpress.com)
Xenopus tropicalis DnaJ heat shock protein family (Hsp40) member B11 (dnajb11), mRNA. (genscript.com)
We have cloned 17 fgf genes from Xenopus tropicalis and have analysed their temporal expression by RT-PCR and spatial expression by whole mount in situ hybridisation at key developmental stages. (manchester.ac.uk)
Three-dimensional folding dynamics of the Xenopus tropicalis genome. (bvsalud.org)
Here, we combined high-throughput chromosome conformation capture and gene silencing to obtain insights into TAD dynamics in Xenopus tropicalis embryos . (bvsalud.org)
Embryos6
Early Pleistocene Olduvai Formation, Tanzania Like many other frogs, they are often used in laboratory as research subjects.Xenopus embryos and eggs are a popular model system for a wide variety of biological studies. (wikipedia.org)
Embryos of zebrafish and Xenopus frogs are abundant, small and free-living. (nih.gov)
The advantages of Xenopus embryos as in vivo models to study human inherited diseases will be presented and their utility for drug discovery screening will be discussed. (nih.gov)
Using transgenic frog technology, I demonstrate that a 580bp cardiac α-actin promoter fragment drives high level expression of a GFP reporter in the heart and skeletal muscle of Xenopus embryos. (ucl.ac.uk)
Chk1 is also transiently activated at the MBT in Xenopus laevis embryos, even when there is no block to DNA replication or damaged DNA. (vt.edu)
For a reverse experiment, DNA methylation in early Xenopus embryos was assessed by MBD affinity capture. (biomedcentral.com)
Genus3
Members of the frog Xenopus genus are versatile model organisms used in developmental biology, cell biology, neuroscience, and toxicology research, among others. (cshlpress.com)
For over a century amphibians from the genus Xenopus have been the organisms of choice to study developmental processes. (biomedcentral.com)
An Xenopus victorianus in nahilalakip ha genus nga Xenopus , ngan familia nga Pipidae . (wikipedia.org)
Species7
All species of Xenopus have flattened, somewhat egg-shaped and streamlined bodies, and very slippery skin (because of a protective mucus covering). (wikipedia.org)
Xenopus species are entirely aquatic, though they have been observed migrating on land to nearby bodies of water during times of drought or in heavy rain. (wikipedia.org)
The females of many species produce a release call, and Xenopus laevis females produce an additional call when sexually receptive and soon to lay eggs. (wikipedia.org)
The Xenopus species are also active during the twilight (or crepuscular) hours. (wikipedia.org)
Xenopus is also a unique system for analyses of genome evolution and whole genome duplication in vertebrates, as different Xenopus species form a ploidy series formed by interspecific hybridization. (wikipedia.org)
The frogs occurring on the islands of Fernando Póo, Principe, Sao Tomé and Annobón represent the species Xenopus andrei (Loumont 1983). (amphibiaweb.org)
An Xenopus victorianus [2] in uska species han Anura nga ginhulagway ni Ahl hadton 1924. (wikipedia.org)
Embryo3
The Xenopus cardiac myosin light chain 2 gene (XMLC2) encodes a contractile protein localised exclusively to the heart of the embryo. (ucl.ac.uk)
Type II collagen expression is therefore temporally regulated by tissue-specific transcription and splicing factors directing the synthesis of distinct molecular forms of the precursor protein in the developing Xenopus embryo. (rupress.org)
However, checkpoints are engaged in Xenopus once the embryo reaches the midblastula transition (MBT). (vt.edu)
Extracts3
However, the wide breadth of Xenopus research stems from the additional fact that cell-free extracts made from Xenopus are a premier in vitro system for studies of fundamental aspects of cell and molecular biology. (wikipedia.org)
Here, we show that Claspin associates with several core replication fork proteins in Xenopus egg extracts. (caltech.edu)
Endogenous MeCP2 and MBD3 were precipitated from Xenopus oocyte extracts and conditions for methylation-specific binding were optimized. (biomedcentral.com)
Oocyte1
Here I investigate the effects of subunit composition on toxin blocking affinity, using three members of the Shaker subfamily of potassium channel α-subunits (Kv1.1, Kv1.2 and Kv1.4), which are known to form heteromultimers in vivo, in the Xenopus oocyte expression system. (aspetjournals.org)
Frog5
A female Xenopus frog injected with a woman's urine was put in a jar with a little water. (wikipedia.org)
Studies using the frog Xenopus have made remarkable contributions to our understanding of fundamental processes such as cell cycle regulation, transcription, translation and many other topics. (cshl.edu)
Transgenic frog technology indicates that the 3kb 5' proximal promoter fragment can direct cardiac muscle-specific reporter expression during Xenopus embryogenesis, reproducing the normal expression pattern of the endogenous gene. (ucl.ac.uk)
In the present study, I examined the effect of benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PEITC), and sulforaphane (SFN) on the accumulation of HO-1, HSP70, HSP30 and HSPB6 in A6 kidney epithelial cells derived from the frog, Xenopus laevis. (uwaterloo.ca)
Here, we document the interplay between rhythmic locomotory and `C-start' escape swimming in Xenopus frog larvae when exposed to hyperthermic conditions. (biologists.com)
Organism4
Xenbase: The Xenopus Model Organism Knowledgebase. (xenbase.org)
Because Xenopus are easy to raise, producing many thousands of eggs per day, these frogs have emerged as a premiere model for understanding of human biology from the fundamental building blocks to the whole organism. (cshl.edu)
For the first time, these nanoparticles were evaluated in an in vivo Xenopus laevis model to study whole organism toxicity and their impact on iron and zinc metabolic pathways. (uea.ac.uk)
This testing approach based on a battery of cellular systems and the use of the Xenopus laevis model could be a viable strategy for studying the toxicity, degradability and ultimately the long-term fate of zinc ferrites in the organism. (uea.ac.uk)
Vivo2
Xenopus has long been an important tool for in vivo studies in molecular, cell, and developmental biology of vertebrate animals. (wikipedia.org)
Thus, Xenopus is a vertebrate model system that allows for high-throughput in vivo analyses of gene function and high-throughput biochemistry. (wikipedia.org)
Frogs2
It is a critical reference for laboratories that currently work with these organisms and will serve as an essential start-up guide for those seeking to start using Xenopus frogs in their research for the first time. (cshlpress.com)
The studies were conducted in zebrafish at Princeton and in African clawed frogs (Xenopus laevis) at Northwestern. (princeton.edu)
Genes1
Expression of two nonallelic type II procollagen genes during Xenopus laevis embryogenesis is characterized by stage-specific production of alternatively spliced transcripts. (rupress.org)
Protein3
Gel shift experiments reveal that the four consensus MEF2 sites in the 5' flanking region of the XMLC2 gene bind Xenopus MEF2D protein. (ucl.ac.uk)
The cardiac α-actin gene is a contractile protein expressed concomitantly in the somitic and cardiac muscle during Xenopus embryogenesis. (ucl.ac.uk)
The final chapter of this thesis investigated characteristics of the Xenopus small heat shock protein HSPB6, a HSP whose levels were not affected by ITC treatment of A6 cells. (uwaterloo.ca)
Transgenesis1
Deletion mapping of the 3kb XMLC2 promoter by Xenopus transgenesis defines the most proximal 708bp as the minimal promoter fragment sufficient to confer high level reporter expression in the embryonic heart. (ucl.ac.uk)
Tadpole1
It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. (ucsd.edu)
Embryogenesis1
The pattern of type II collagen expression during Xenopus laevis embryogenesis has been established after isolating specific cDNA and genomic clones. (rupress.org)
Gene3
We analyzed genome-wide changes in DNA methylation and their relationship to gene regulation in the brain of Xenopus tadpoles during metamorphosis, a thyroid hormone-dependent developmental process. (nih.gov)
Extensive DNA sequence comparisons between various Xenopus histone gene clusters were undertaken. (bl.uk)
During the course, the students will analyze any phenotypes generated from CRISPR/Cas9 based gene depletion while learning the diverse array of techniques available in Xenopus . (cshl.edu)
Physiology1
There are protocols for analyses of Xenopus immunology, neurobiology, metamorphosis, and adult physiology. (cshlpress.com)
Genome1
Here, we will review the rapid progress made in adapting these novel genome editing tools to Xenopus. (nih.gov)
Zebrafish1
Being a tetrapod, Xenopus complements zebrafish as an indispensable non-mammalian animal model for the study of human disease pathologies and the discovery of novel therapeutics for inherited diseases. (nih.gov)
This new laboratory manual provides a comprehensive collection of experimental procedures for research using Xenopus . (cshlpress.com)
Research2
Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative. (genscript.com)
Since a great deal of the molecular and cellular research with Xenopus is applicable to humans, an increase in our knowledge of the effects of ITCs is of importance given their potential role as therapeutic compounds in the treatment of cancer, neurodegenerative diseases and bacterial and viral infections. (uwaterloo.ca)
We characterized Xenopus Claspin as a kinase substrate of DDK which forms a stable nuclear complex with Cdc7 and Drf1 under both arrested and unperturbed replication conditions. (caltech.edu)
Development2
The rate of Xenopus development is influenced by temperature. (xenbase.org)
Xenopus is remarkable for studying development and disease, including birth defects, cancer, and stem cell biology. (cshl.edu)
Cell2
Xenopus is increasingly being used as imaging test-bed to investigate the roles of cytoskeleton and intracellular trafficking in cell biological and morphogenetic contexts. (cshl.edu)
The power of Xenopus can be leveraged when live-cell fluorescence imaging is combined with microsurgery, grafting, and dissociated cell culture. (cshl.edu)
Animal1
The Xenopus egg is divided into two roughly equal halves: the animal and vegetal hemispheres. (xenbase.org)