Showed antitumor activityProteinsVivoSubcutaneousVitroTumorsAortic ringNudeApoptosisAMPLIFICATION ASSAYAntibodiesPatient-derivedMurineMelanomaEndothelial CellsActivityImmunityMiceInhibitorsPancreaticInhibition of tumorAntioxidantMigrationColonRobustToxicitySystemicCellGrowthTubulinInvestigateEffectsPhagocytosisExperimentalAngiogenesisMouse modelsCellsEffectResults
Showed antitumor activity1
- In mouse and rat xenograft tumor models, TAK-285 showed antitumor activity against cancers that expressed HER2 or EGFR. (jcancer.org)
Proteins1
- Six of the interacting proteins were cellular chaperones, including 3 members of the heat shock protein-90 (Hsp90) family, and so the effect of Hsp90 modulation on the antitumor activity of topoisomerase II drugs was tested using the sulforhodamine B assay, clonogenic assays and a xenograft model. (uea.ac.uk)
Vivo12
- Scholars@Duke publication: Targeting Dormant Ovarian Cancer Cells In Vitro and in an In Vivo Mouse Model of Platinum Resistance. (duke.edu)
- By live in vivo imaging, ovarian cancer xenograft tumors were reduced after primary treatment with carboplatin. (duke.edu)
- Moreover, fucoidan inhibited in vivo angiogenesis and micrometastasis in a transgenic zebrafish model. (tmu.edu.tw)
- Next, we will test the combination therapy in xenograft mouse models of KRAS mutant cancer to evaluate for enhanced anti-tumor immunity in vivo (Aim 2). (hhs.gov)
- MATERIALS AND METHODS: We examined the effects of IMB5036 on cancer cells by in vitro assays, a molecular docking model and in vivo tumour models. (bvsalud.org)
- Multiple mouse xenograft studies were performed to assess in vivo antitumor activity. (usuhs.edu)
- The present study aimed to investigate the ability of H 2 Mab-41 to induce antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) in dog HER2 (dHER2)-overexpressed cell lines , and thus exert its antitumor activity against dHER2-overexpressed tumors in vivo . (populationhealthsciences.org)
- The effects of the low-dose chidamide and bortezomib combination reduced the growth on gastric cancer in vivo were investigated by using a subcutaneous tumor mouse model. (dovepress.com)
- Finally, the antitumor effect of the leaves ethanolic extract was evaluated using in vivo human tumor xenograft model. (emanresearch.org)
- Here, we investigated the potential targeting of MEK pathway by exploiting highly reliable in vitro and in vivo pre-clinical models of melanomas based on melanospheres, as melanoma initiating cells (MIC) surrogates. (biomedcentral.com)
- The in vivo efficacy of CAR-M was characterized using metastatic solid tumor xenograft models. (bmj.com)
- In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)
Subcutaneous3
- TAK-285 was as effective as lapatinib in antitumor activity in a mouse subcutaneous BT-474 breast cancer xenograft model. (jcancer.org)
- Systemic delivery of HTL-001 resulted in improved control of subcutaneous murine and human xenograft tumours and improved survival in a murine orthotopic model. (biomedcentral.com)
- Conclusions: PET imaging studies with [89Zr]Zr-DFO-anti-γH2AX-TAT following α- and β-particle PRIT in a BxPC3 PDAC subcutaneous xenograft mouse model allowed the monitoring of tumor radiobiological response to treatment. (ox.ac.uk)
Vitro4
- To investigate potential synergy between KRAS inhibitors and anti-CD47 antibodies, we have developed a novel in vitro co-culture assay to evaluate macrophage-mediated destruction of cancer. (hhs.gov)
- SRF231 exerts antitumor activity in vitro through both phagocytosis and cell loss of life in a way reliant on the activating Fc-gamma receptor (FcR), Compact disc32a. (nihvp.org)
- The anti-tumor efficacy of CAR-M in vitro was monitored by quantifying killing, phagocytosis, and cytokine production in co-culture assays with HER2+ tumor cell lines. (bmj.com)
- Tetrazolium reduction and resazurin assays are the mainstay of routine in vitro toxicity batteries. (cdc.gov)
Tumors2
- The cytotoxic activity of SAR408701 was assessed in CEACAM5-expressing tumor cell lines and using patient-derived xenograft mouse models of CEACAM5-positive tumors. (nih.gov)
- PD0325901 treatment, dramatically inhibited growth of melanosphere-generated xenografts and determined impaired tumor vascularization of both mutated- and wild type-BRAF tumors, in the absence of mice toxicity. (biomedcentral.com)
Aortic ring3
- LP-261 was also tested in a rat aortic ring assay, from 20 nM to 10 μM. (usuhs.edu)
- LP-261 is a very potent inhibitor of angiogenesis, preventing microvessel outgrowth in the rat aortic ring assay and HUVEC cell proliferation at nanomolar concentrations. (usuhs.edu)
- Hexane extract of the stem bark and ethanolic leaves extract profoundly inhibited the sprouting of microvessels (by 89.56% and 87.12% respectively) in rat aortic ring assay. (emanresearch.org)
Nude2
- Nude rats bearing the LC-6 JCK tumor xenograft (LC-6 rats) exhibited high bone turnover and HHM. (unboundmedicine.com)
- and examined the anti-tumor activity of both miR-26a and miR-144 inside a xenograft nude mouse model of ESCC. (revoluciondelosgladiolos.org)
Apoptosis2
- Bottom line SRF231 elicits antitumor activity via phagocytosis and BKI-1369 apoptosis regarding macrophage engagement in a way reliant on the FcR, Compact disc32a. (nihvp.org)
- Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
AMPLIFICATION ASSAY1
- A small-molecule library enriched for FDA-approved drugs was screened with a high-throughput, ligation-mediated amplification assay with a fluorescent, bead-based detection. (broadinstitute.org)
Antibodies1
- We will use this assay to measure synergy between KRAS inhibitors and anti-CD47 antibodies (Aim 1). (hhs.gov)
Patient-derived1
- Treatment of patient-derived xenografts reveals synergistic antitumoral and antimetastatic effects of the dual ET-1R antagonist macitentan in combination with cisplatinum, shutting-down the β-arr1-mediated YAP/mutp53 transcriptional programme. (mcmaster.ca)
Murine1
- Using transcriptomic profiling of isolated bone lining cell subtypes from a murine myeloma model, we find that bone morphogenetic protein (BMP) signalling is upregulated in stromal progenitor cells. (ox.ac.uk)
Melanoma1
- We tracked CD4 + and CD8 + T cells in wild-type mice, in immunodeficient recipients reconstituted with monoclonal populations of OT-II and OT-I T cells, and in a B16 melanoma model. (populationhealthsciences.org)
Endothelial Cells1
- In order to confirm the effect of the extract on motility of human endothelial cells, cell migration assay was conducted. (emanresearch.org)
Activity8
- Our studies suggest that investigational drugs such as TAK-285 that have strong antitumor activity and are not Pgp substrates may be useful in the development of agents with the potential to treat brain metastases. (jcancer.org)
- AIMS: IMB5036 is a pyridazinone compound with antiproliferative and antitumour activity against hepatoma and pancreatic cancer. (bvsalud.org)
- Here, its antitumor activity and the underlying mechanism were investigated. (bvsalud.org)
- xenograft models, as well as the system of antitumor activity was assessed using macrophage and cytokine infiltration analyses following SRF231 treatment. (nihvp.org)
- Robust antitumor activity takes place across multiple hematologic xenograft versions either as an individual agent or in conjunction with rituximab. (nihvp.org)
- Macrophage depletion leads to reduced SRF231 antitumor activity, underscoring a mechanistic function for macrophage engagement by SRF231. (nihvp.org)
- Previously, an anti-HER2 mAb, H 2 Mab-41 (IgG 2b , kappa), was developed in our laboratory and its antitumor activity was demonstrated in mouse xenograft models of human colon cancer. (populationhealthsciences.org)
- Some Mek inhibitors showed promising antitumor activity, although schedules and doses associated with low systemic toxicity need to be defined. (biomedcentral.com)
Immunity1
- THZ1 boosted antitumor immunity by recruiting infiltrating CD8 + T cells and synergized with antiPD-1 therapy. (biomedcentral.com)
Mice3
- The Public Repository of Xenografts Enables Discovery and Randomized Phase II-like Trials in Mice. (umassmed.edu)
- IMB5476 also inhibited the growth of drug-resistant KBV200 xenografts in mice. (bvsalud.org)
- Methods: Mice bearing BxPC3 tumor xenografts were treated with α- and β-particle pretargeted radioimmunotherapy (PRIT), external beam radiotherapy (EBRT), or sham-treated (vehicle). (ox.ac.uk)
Inhibitors1
- These data highlight the utility of new RET+ models to elucidate differences in response to tyrosine kinase inhibitors and downstream signaling regulation. (nih.gov)
Pancreatic2
- 89Zr-PET imaging of DNA double-strand breaks for the early monitoring of response following α- and β-particle radioimmunotherapy in a mouse model of pancreatic ductal adenocarcinoma. (ox.ac.uk)
- Suppressive effects of vascular endothelial growth factor-B on tumor growth in a mouse model of pancreatic neuroendocrine tumorigenesis. (emanresearch.org)
Inhibition of tumor3
- Complete inhibition of tumor growth was achieved in the PC3 xenograft model and shown to be schedule dependent. (usuhs.edu)
- Excellent inhibition of tumor growth in the SW620 model was observed, comparable with paclitaxel. (usuhs.edu)
- Furthermore, intraperitoneal administration of H 2 Mab-41 in mouse xenograft models of CHO/dHER2 resulted in significant inhibition of tumor development compared to the control mouse IgG. (populationhealthsciences.org)
Antioxidant1
- The active compound curcumin is believed to have a wide range of biological effects including anti-inflammatory, antioxidant, antitumor, antibacterial, and antiviral activities, which have shown a lot of potential for use in clinical medicine. (blogspot.com)
Migration1
- Transwell and wound healing assays were used to evaluate migration and invasion. (referencecitationanalysis.com)
Colon1
- LP-261 was tested as a single agent in colon adenocarcinoma (SW620) and prostate cancer (LNCaP and PC3) xenografts, evaluating several different dosing schedules. (usuhs.edu)
Robust1
- In cell coculture assays, ICOVIR-15K-cBiTE-mediated oncolysis resulted in robust T-cell activation, proliferation, and bystander cell-mediated cytotoxicity. (aacrjournals.org)
Toxicity1
- The use of traditional Chinese medicinal herbs with low toxicity as alternatives to antitumor drugs has attracted widespread attention [ 5 ]. (hindawi.com)
Systemic2
- Background Macrophages expressing chimeric antigen receptors (CAR-M) have been shown to reduce tumor burden, remodel the tumor microenvironment (TME), and coordinate a systemic immune response in pre-clinical solid tumor models. (bmj.com)
- In ZR75.1 breast carcinoma xenografts virus distribution was different, with no evidence of systemic overflow leading to hepatic transduction following IT injection. (ox.ac.uk)
Cell4
- In this study, tumorigenic potential was assessed using anchorage-independent growth assays and spheroid-forming capacity, which was determined with ovarian cancer cell lines as well as primary ovarian cancers. (duke.edu)
- ARA-C reduced EWS/FLI protein abundance and accordingly diminished cell viability and transformation and abrogated tumor growth in a xenograft model. (broadinstitute.org)
- The aim of this study was to investigate the antitumor effect of chidamide in combination with bortezomib on gastric cancer cell lines. (dovepress.com)
- First, the sensitivity and IC 50 values of chidamide and bortezomib in several gastric cancer cell lines (MGC-803, BGC-823, SGC-7901, and MKN45) were measured using the CCK-8 assay. (dovepress.com)
Growth3
- TAK-285 was examined in a model of breast cancer brain metastasis using direct intracranial injection of BT-474-derived luciferase-expressing cells and showed greater inhibition of brain tumor growth compared to animals treated with lapatinib. (jcancer.org)
- Oral LP-261 is very effective at inhibiting tumor growth in multiple mouse xenograft models and is well tolerated. (usuhs.edu)
- We characterized shRNA-modified CAR-M using a model anti- human epidermal growth factor receptor 2 (HER2) CAR paired with shRNA targeting SIRPα. (bmj.com)
Tubulin1
- Competition assay indicated that it bound to tubulin at the colchicine pocket. (bvsalud.org)
Investigate2
- Finally, we will investigate the effects of the combination therapy in immunocompetent, syngeneic tumor models to understand how the combination therapy alters interactions between the innate and adaptive immune systems. (hhs.gov)
- CCK-8 and colony formation assay were used to investigate proliferation. (referencecitationanalysis.com)
Effects2
- Therefore, finding effective antitumor drugs with few side effects is still necessary. (hindawi.com)
- Together, L. japonica fucoidan exhibits potent antitumor effects by its attenuation of invasiveness and proangiogenesis in TNBC. (tmu.edu.tw)
Phagocytosis1
- The generally recognized eat-me/dont-eat-me style of Compact disc47/SIRP legislation of phagocytosis is normally a two-signal model, where macrophages need the lack of SIRP signaling (indication 1) aswell as the current presence of an activating or eat-me indication (indication 2). (nihvp.org)
Experimental1
- The control group was given routine pituitary tumor care, and the "one disease, one product" nursing model was used on the experimental group. (bvsalud.org)
Angiogenesis1
- Here, we report the results of testing in multiple mouse xenograft models and angiogenesis assays, along with bioavailability studies. (usuhs.edu)
Mouse models2
- More recently, we successfully developed and evaluated in PDAC mouse models a targeted α-therapy strategy with high clinical translation potential. (ox.ac.uk)
- Results: PET imaging studies in BxPC3 PDAC mouse models demonstrated increased uptake of [89Zr]Zr-DFO-anti-γH2AX-TAT (6.29 ± 0.15 %IA/g) following β-PRIT in BxPC3 PDAC xenografts as compared to the saline control group (4.58 ± 0.76 %IA/g) and EBRT control group (5.93 ± 0.76 %IA/g). (ox.ac.uk)
Cells1
- Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)
Effect1
- We aim to expedite the clinical translation of the developed radioimmunotherapy approaches by investigating the early therapeutic response and effect of radiation therapy in a PDAC mouse model via PET imaging. (ox.ac.uk)
Results1
- The current investigation aimed to demonstrate how interpretation of results from several standard cytotoxicity and proliferation assays vary in dependence on contributions from the pentose phosphate pathway (PPP). (cdc.gov)