• pseudorabies virus
  • 9. The immunogenic composition of claim 4, further comprising at least one pathogen antigen selected from the group consisting of antigen of Swine influenza virus (SIV), antigen of porcine reproductive and respiratory syndrome virus (PRRSV), antigen of mycoplasma, antigen of porcine parvovirus (PPV), antigen of erysipelas, and antigen of pseudorabies virus. (patentsencyclopedia.com)
  • During the 1930s, he studied on a fellowship at the Rockefeller Institute for Medical Research in Princeton, New Jersey mentored by Richard Shope, performing research on vaccines and viruses, including pseudorabies virus and lymphocytic choriomeningitis virus (LCM). (wikipedia.org)
  • Receptors
  • To avoid this, we constructed CHO and PK15 cell lines recombinantly expressing the PRRSV receptors involved in virus entry into macrophages, sialoadhesin (Sn) and CD163 (CHOSn-CD163 and PK15Sn-CD163) and evaluated their potential for production of PRRSV. (nih.gov)
  • Tissues
  • Animal viruses, in particular, can cause microscopic or macroscopic degenerative changes or abnormalities in host cells and in tissues called cytopathic effects, cytopathic effects may be lethal, but plaque formation from cell lysis does not always occur. (blogspot.com)
  • viral
  • Inhibition of CKII kinase in infected primary hepatocytes and destruction of the identified CKII recognition site in the viral polymerase impaired virus production. (hu-berlin.de)
  • After binding to molecules on the cell surface, the virion is endocytosed and enters the endoplasmic reticulum - a behavior unique among known non-enveloped viruses - where the viral capsid structure is likely to be disrupted by action of host cell disulfide isomerase enzymes. (wikipedia.org)
  • Certain transcription factors present in the early promoter sequences of the JC virus can induce trophism and viral proliferation that leads to PML. (wikipedia.org)
  • Although JC virus infection is classically associated with white matter demyelination and PML pathogenesis, recent literature has identified viral variants as etiological agents of other novel syndromes. (wikipedia.org)
  • biological
  • On orders from Himmler and Blome, the Deputy Reich Health Leader and head of the German biological warfare program, Traub worked on weaponizing foot-and-mouth disease virus, which has been reported to have been dispersed by aircraft onto cattle and reindeer in Russia. (wikipedia.org)
  • As a noted German authority on viruses he was asked to consult on their animal disease program from a Biological Warfare perspective. (wikipedia.org)
  • This can include samples of a microorganism, virus or toxin (from a biological source) that can affect human health. (wikipedia.org)
  • The Use of the Developing Egg in Virus Research (Special Report No, 220), London: Medical Research Council, 1936 Biological Aspects of Infectious Disease Cambridge: Cambridge University Press, 1940. (wikipedia.org)
  • Enzyme, Antigen and Virus: a Study of Macromolecular Pattern in Action, Cambridge: Cambridge University Press, 1956 With W.M. Stanley The Viruses: Biochemical, Biological and Biophysical Properties, 3 volumes, New York:Academic Press, 1959 With M. Freeman, A.V. Jackson and M. Lush, The Production of Antibodies: A Review and Theoretical Discussion (Monograph of the Walter and Eliza Hall Institute). (wikipedia.org)
  • measles virus
  • Her most significant contribution to the research was her research on human amnion cells with Dr. Milan V. Milovanovic and how they supported the growth of the measles virus. (wikipedia.org)
  • In this research, the team was able to propagate the measles virus throughout 28 serial passages in human amnion cells. (wikipedia.org)
  • After the 28th passage in human amnion cells, the measles virus was then inoculated into chick embryos. (wikipedia.org)
  • In 1959, Mitus was the lead author of an article entitled "Persistence of Measles Virus and Depression of Antibody Formation in Patients with Giant-Cell Pneumonia after Measles" published in The New England Journal of Medicine. (wikipedia.org)
  • The main conclusion from this paper was that in a proportion of giant-cell pneumonia cases, the measles virus was found to be an etiologic agent. (wikipedia.org)
  • Overall, Mitus published at least 9 peer-reviewed journal articles on measles and the measles virus. (wikipedia.org)
  • vaccines
  • For analysis of humoral immunity, groups of five Balb/c mice were immunized at day 0 (1×) or day 0 and 14 (2×) with 10 μg of single inactivated vaccines or 20 μg of co-formulated INAC-RV-GP + INAC-RV-HC50 (10 μg each virus). (mglurpathway.com)
  • Biohazard Level 4: Viruses that cause severe to fatal disease in humans, and for which vaccines or other treatments are not available, such as Bolivian hemorrhagic fever, Marburg virus, Ebola virus, Lassa fever virus, Crimean-Congo hemorrhagic fever, and other hemorrhagic diseases and rishibola. (wikipedia.org)
  • As with other live-virus vaccines, immunity initiated by OPV is probably lifelong. (wikipedia.org)
  • In 1960, it was determined that the rhesus monkey kidney cells used to prepare the poliovirus vaccines were infected with the SV40 virus (Simian Virus-40). (wikipedia.org)
  • genome
  • The genome HIV virus contains which the following? (mbbsdost.com)
  • Parad's dominance in Emu's body helped the boy rise to fame as "Genius Gamer M" (天才ゲーマーM, Tensai Gēmā Emu) until he was abducted by Next Genome scientists to extract the Bugster Viruses for Kuroto. (wikipedia.org)
  • Ranaviruses are large icosahedral DNA viruses measuring approximately 150 nm in diameter with a large single linear dsDNA genome of roughly 105 kbp which codes for around 100 gene products. (wikipedia.org)
  • MR is the process by which two, or more, virus genomes containing otherwise lethal damage interact within an infected cell to form a viable virus genome. (wikipedia.org)
  • reviewed numerous examples of MR in different viruses, and suggested that MR is a common form of sexual interaction in viruses that provides the advantage of recombinational repair of genome damages. (wikipedia.org)
  • Vaccinia contains within its genome genes for several proteins that give the virus resistance to interferons : K3L is a protein with homology to the protein eukaryotic initiation factor 2 (eIF-2alpha). (wikipedia.org)
  • smallpox
  • Smallpox was the first disease to be widely prevented by vaccination due to pioneering work by the English physician and scientist Edward Jenner in the eighteenth century using cowpox virus. (wikipedia.org)
  • Variola virus (smallpox) is an agent that is worked with at BSL-4 despite the existence of a vaccine, as it has been eradicated. (wikipedia.org)
  • diseases
  • No assurance of 100% fruit baring trees, not resistant to virus diseases. (island.lk)
  • From 1942 to 1948, Traub worked as lab-chief at the Reich Research Institute for Virus Diseases of Animals (German: Reichsforschungsanstalt für Viruskrankheiten der Tiere) on Riems Island (German: Insel Riems), a German animal virus research institute in the Baltic sea, now named the Friedrich Loeffler Institute. (wikipedia.org)
  • Traub discussed work done at the Reich Research Institute for Virus Diseases of Animals on Riems Island during World War II for the Nazis, and work done after the war there for the Russians. (wikipedia.org)
  • The virus causes PML and other diseases only in cases of immunodeficiency, as in AIDS or during treatment with drugs intended to induce a state of immunosuppression (e.g. organ transplant patients). (wikipedia.org)
  • Melbourne: Macmillam, 1942 Virus as Organism: evolutionary and ecological aspects of some human virus diseases. (wikipedia.org)
  • hemorrhagic
  • The risk for person-to-person transmission of hemorrhagic fever viruses is highest during the latter stages of illness, which are characterized by vomiting, diarrhea, shock, and often hemorrhage. (cdc.gov)
  • or 3) worked in a laboratory or animal facility that handles hemorrhagic fever viruses. (cdc.gov)
  • vaccine virus
  • We have made ten attempts to cultivate vaccine virus in tissue extracts prepared according to the method described by Eagles and Kordi (4). (rupress.org)
  • Nevertheless, one cannot examine the results of our work recorded in the six tables without recognizing the fact that in the types of media used the presence of viable cells appears to be essential for the multiplication of vaccine virus. (rupress.org)
  • Comparison of both cell lines showed that the PK15Sn-CD163 cell line gave in general better results than the CHOSn-CD163 cell line.Mutations induced by growth on this cell lines were either absent or minimal and located outside known neutralizing epitopes.Together, the results show that this cell line can be used to produce vaccine virus and for PRRSV virus isolation. (nih.gov)
  • Together, the results show that this cell line can be used to produce vaccine virus and for PRRSV virus isolation. (nih.gov)
  • infectious virus
  • Second, VSV was incubated in LC phases of either C(14)AO + D or DSCG for 4 h, and the concentration (titer) of infectious virus in the LC was determined by dilution into cell culture medium and subsequent inoculation of HeLa cells. (biomedsearch.com)
  • titer
  • Furthermore, the virus titer of all 5 PRRSV strains produced after passaging in PK15Sn-CD163 cells was similar to the virus titer of those strains produced in Marc-145 cells. (nih.gov)
  • SV40
  • The JC virus or John Cunningham virus is a type of human polyomavirus (formerly known as papovavirus) and is genetically similar to BK virus and SV40. (wikipedia.org)
  • protein
  • Control experiments using cell lysates with equivalent protein concentrations but no virus did not perturb the uniform alignment of the LC. (biomedsearch.com)
  • systemic
  • The Ranaviruses, like the Megalocytiviruses, are an emerging group of closely related dsDNA viruses which cause systemic infections in a wide variety of wild and cultured fresh and saltwater fishes. (wikipedia.org)
  • Studies
  • and Lambert, R.A. (1913) "Studies on the cultivation of the virus of vaccinia" J. Inf Dis. (wikipedia.org)
  • Several studies since 2000 have suggested that the virus is also linked to colorectal cancer, as JCV has been found in malignant colon tumors, but these findings are still controversial. (wikipedia.org)
  • While working with her husband and Goodpasture, she conducted studies in the "nature, infectivity, and purification of fowl-pox virus, and the character of the changes it induced on experimental infection of fowls," which became the forerunner in the cultivation of viruses. (wikipedia.org)
  • doses
  • When the current formulation of IPV is used, 90% or more of individuals develop protective antibody to all three serotypes of polio virus after two doses of inactivated polio vaccine (IPV), and at least 99% are immune to polio virus following three doses. (wikipedia.org)
  • antigen
  • Further research is needed to determine the exact etiological role of T-antigen, but there seems to be a connection to the early initiation of the active virus from its archetypal dormant state. (wikipedia.org)
  • interaction
  • If the cells were treated with neuraminidase to remove cis-acting sialic acids that hinder the interaction of the virus with Sn, the amount of infected cells with macrophage grown virus increased. (nih.gov)
  • for example, the JC virus is believed to require interaction with the 5HT2A receptor and the Merkel cell virus with heparan sulfate. (wikipedia.org)
  • virions
  • Additionally, analysis of the sub-cellular localization of JC CPN virions in nuclei, cytoplasm, and axons suggests that the virus may travel through axons to increase infectivity. (wikipedia.org)
  • enzyme
  • Hu-man-immunodeficiency-virus infections in infants negative for anti-HIV by enzyme-linked immunoassay. (springer.com)
  • Borkowsky W, Krasinski K, Paul D, Moore T, Bebenroth D, Chandwani S (1987) Humanimmundeficiency-virus infections in infants negative for anti-HIV by enzyme-linked immunoassay. (springer.com)
  • cause
  • 3. The porcine circovirus type 2 (PCV2) virus of claim 1, wherein the PCV2 virus is able to cause cytopathic effect (CPE) in PK-15 cell line or its derivative cell lines. (patentsencyclopedia.com)
  • While the vaccine is made with a live virus which can cause side effects, these are far fewer and less serious than the sickness and death caused by measles itself. (wikipedia.org)
  • Porcine
  • Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163. (nih.gov)
  • Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. (nih.gov)
  • however
  • However, since virus entry in Marc-145 cells is different compared to entry in primary macrophages, specific epitopes associated with virus entry could potentially alter upon growth on Marc-145 cells. (nih.gov)
  • However, it was observed that Marc-145 grown virus infected the cells more efficiently than macrophage grown virus. (nih.gov)
  • However, in general virus-cell interactions are mediated by commonly occurring molecules on the cell surface, and therefore are likely not a major contributor to individual viruses' observed cell-type tropism. (wikipedia.org)
  • proteins
  • Analysis of the sequence of the structural proteins of original virus and virus grown for 5 passages on PK15Sn-CD163 cells showed either no amino acid (aa) changes (VR-2332 and 07V063), one aa (LV), two aa (08V194) or three aa (08V204) changes. (nih.gov)
  • membrane
  • For convenience, the myxoma virus grows well on the chorioallantoic membrane, whereas the mumps virus prefers the allantoic cavity. (blogspot.com)
  • genes
  • Of all herpes viruses, HCMV harbors the most genes dedicated to altering (evading) innate and adaptive immunity in the host and represents a lifelong burden of antigenic T cell surveillance and immune dysfunction. (wikipedia.org)
  • cytoplasm
  • Poxviruses are unique among DNA viruses because they replicate only in the cytoplasm of the host cell, outside of the nucleus. (wikipedia.org)
  • cell
  • The clonal cell lines suspended in suitable media are infected with any desired virus which replicates inside the multiplying cells. (blogspot.com)
  • One aim was to enhance HBV production of the cell line HepG2.2.15 by cultivation on microcarrier substrate. (hu-berlin.de)
  • Natural history of Human Immunodeficiency Virus in-fections in hemophiliacs: Effects of T-cell sub-sets, Platelet Counts, and Age. (springer.com)
  • hu-man T-cell lymphotropic virus type III (HTLV-III) embryopathie. (springer.com)
  • Using these approaches, we found that the LC containing C(14)AO + D caused inactivation of virus as well as cell death. (biomedsearch.com)
  • Marion RW, Wiznia AA, Hutcheon RG, Rubinstein A (1986) Human T-Cell lymphotropic virus type III (HTLV III) embryopathy. (springer.com)
  • A PRRSV susceptible cell line was constructed that can grow virus to similar levels compared to currently available cell lines. (nih.gov)
  • For Marc-145 grown virus infection of CHOSn-CD163 cells, little difference was observed between different cell densities and days post seeding, although a density of 200 000 cells/mL and inoculation at 2 days post seeding seemed a little more efficient. (nih.gov)
  • infants
  • Blanche S, Rouzioux C, Guihard Moscato ML (1989) A prospective study of infants born to women seropositive for human immunodeficiency virus type 1. (springer.com)