• assays
  • To develop a better understanding of the significance of low dose effects, in the present study, we developed quantitative methods for the detection of biologically active aflatoxin B 1 (AFB1) in Vero cells by two independent assays: the green fluorescent protein (GFP) assay, as a measure of protein synthesis by the cells, and the microculture tetrazolium (MTT) assay, as a measure of cell viability. (mdpi.com)
  • The cytotoxic and anti-genotoxic activities were evaluated against Vero cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and comet assays. (deepdyve.com)
  • proteins
  • The second stage of the research involved the use of immunofluorescent staining to locate viral proteins within infected cells in order to inform future process optimization. (uwaterloo.ca)
  • Infection
  • The fluorescence which the HSV2012-121 Vero cells displayed on infection with ACAM529 did not allow for effective differentiation between infected and uninfected cells. (uwaterloo.ca)
  • Staining and counting of the resulting plaques was used to establish a required initial infection titer between 8.3x104 and 1.2x106 PFU/mL for future cell insert experiments. (uwaterloo.ca)
  • strains
  • iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus. (sigmaaldrich.com)
  • To dissect out the underlying pathogenic mechanism of virulent PEDV and clarify the differences between virulent and CV777 vaccine strain-like PEDV infections, we performed an iTRAQ-based comparative quantitative proteomic study of Vero cells infected with both PEDV strains. (sigmaaldrich.com)
  • genome
  • The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014. (wikipedia.org)
  • Chromosome 12 of Vero cells has a homozygous ~9-Mb deletion, causing the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitors CDKN2A and CDKN2B in the genome. (wikipedia.org)
  • The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus. (wikipedia.org)
  • MR is the process by which two, or more, virus genomes containing otherwise lethal damage interact within an infected cell to form a viable virus genome. (wikipedia.org)
  • membrane
  • The outer viral envelope of the virion is derived by budding from domains of host cell membrane into which the GP spikes have been inserted during their biosynthesis. (wikipedia.org)
  • tcdE has been speculated to facilitate release of TcdA and TcdB through lytic activity on the bacterial cell membrane. (wikipedia.org)
  • viral
  • Viral life cycles vary in their precise details depending on the type of virus, but they all share a general pattern: Attachment to a host cell. (wikipedia.org)
  • Replication of viral components using host-cell machinery. (wikipedia.org)
  • virion
  • During its replication cycle, VV produces four infectious forms which differ in their outer membranes: intracellular mature virion (IMV), the intracellular enveloped virion (IEV), the cell-associated enveloped virion (CEV) and the extracellular enveloped virion (EEV). (wikipedia.org)
  • fluorescence
  • Treatment of the cells with Sodium Butyrate (2mM NaBu added at the time of seeding) resulted in a 16.93-fold difference between infected and uninfected cells' fluorescence. (uwaterloo.ca)
  • entry
  • Receptor binding is the first step required for entry into the cell via endocytosis in an acidic endosome. (wikipedia.org)
  • study
  • Conclusion & Significance: Our fluorescent study was not sensitive enough to detect differences in NLS-transfected-cells under different filters. (omicsonline.org)
  • A recent study demonstrates that the CROPs determine the potency of TcdA through interactions with structures on the cell surface. (wikipedia.org)
  • produce
  • Viruses cannot reproduce on their own, and instead propagate by subjugating a host cell to produce copies of themselves, thus producing the next generation. (wikipedia.org)
  • Response
  • Rasooly R, Hernlem B, He X, Friedman M. Non-Linear Relationships between Aflatoxin B 1 Levels and the Biological Response of Monkey Kidney Vero Cells. (mdpi.com)
  • targets
  • This makes it difficult to find targets for the drug that would interfere with the virus without also harming the host organism's cells. (wikipedia.org)
  • it is also produced in MDCK cells and targets the same Influenza subtypes. (wikipedia.org)
  • specifically
  • The B subunit is a pentamer that binds to specific glycolipids on the host cell, specifically globotriaosylceramide (Gb3). (wikipedia.org)
  • presence
  • Additional studies showed that heat does not affect the stability of AFB1 in milk and that the Vero cell model can be used to determine the presence of bioactive AFB1 in spiked beef, lamb and turkey meat. (mdpi.com)
  • target
  • The carboxy C-terminal domain contains repeating units that are responsible for receptor binding on target cell surfaces. (wikipedia.org)