• By Mahima Devkota , Kathmandu, June 25: The government is all set to administer the second dose of China-made Vero Cell vaccine against COVID-19, for the people age 60 to 64 from July 6. (risingnepaldaily.com)
  • The people who were given the Vero Cell vaccine against COVID-19 are found to have developed 72.4 percent antibody, more than those who have been administered Covishield. (emicrowatch.com)
  • F6B1.1) HeLa cells Immortalised cell line History and Characterization of the Vero Cell Line -- A Report prepared by CDR Rebecca Sheets, Ph.D., USPHS CBER/OVRR/DVRPA/VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12, 2000 OPEN SESSION www.fda.gov pdf Yasumura Y, Kawakita M (1963). (wikipedia.org)
  • The government received 1.2 million doses Vero Cell vaccines produced by the Beijing Institute of Biological Products out of the agreed 59.36 million doses to be purchased with the loan assistance from the Asian Development Bank through the COVAX. (spotlightnepal.com)
  • The procured Vero Cell vaccines are stored at central storage, Teku. (spotlightnepal.com)
  • The Vero Cell vaccines that have been purchased are kept at Teku's central storage. (tyrocity.com)
  • Vero cells grow adherently and are susceptible to many viruses, and this broad susceptibility has prompted the development of corresponding Vero cell-based vaccines. (cytoniche.com)
  • To this day, a variety of Vero cell-based vaccines have been marketed. (cytoniche.com)
  • In modern biotechnology and drug design large-scale cell cultures are necessary tools for the production of diverse recombinant proteins, such as Herceptin™, Enbrel™ or vaccines against the influenza virus strains H5N1 and H1N1 like Celvapan™ [ 1 - 7 ]. (biomedcentral.com)
  • There might be different results from the two types of vaccines as the research has taken place long after Covishield was administered to people as compared to Vero Cell," said senior research officer Dr Megha Nath Dhimal. (emicrowatch.com)
  • Among the population getting a full dose of vaccines, 85.2 percent had been given Covishield vaccines, 14.5 percent had been given Vero Cell and 0.2 percent had received other vaccines. (emicrowatch.com)
  • Vero cells are used to establish cell lives which help in production of vaccines. (tribuneindia.com)
  • Bead enzyme-linked immunosorbent assay and Vero cell assay were performed to detect and evaluate the cytotoxic effect of the Shiga toxins produced by the strains. (cdc.gov)
  • Lonza now has expertise in different cell and vector types, constructs, process and assay development, and platform technologies for both clinical and commercial manufacturing. (bioprocessintl.com)
  • MTT assay was employed to study the anticancer activity against cancer cell lines Hep-G2, MCF7 and normal VERO cell lines. (google.com)
  • Crude methanolic extract of G. corticata had significant anticancer activity followed by E. antenna and E. linza on cancer cell lines Hep-G2, MCF7 and normal VERO cell lines by MTT assay. (google.com)
  • Infectious virus was detected by Vero cell culture and plaque assay. (medscape.com)
  • Human ACE2 gene was integrated into the chromosomes of Vero cells to form an engineered cell line, hACE2-Vero, that stably expresses ACE2 on the cell surface. (creative-biolabs.com)
  • People having been administered Vero Cell have developed more antibodies as opposed to Covishield as the research has taken place shortly after the vaccination. (emicrowatch.com)
  • Cells are subsequently fixed, permeabilized, and stained intracellularly with antibodies for 30 min on ice. (jax.org)
  • Immunologic techniques such as staining with tagged monoclonal antibodies should be used to confirm cytopathic effects on cell cultures. (medscape.com)
  • Nepal received 1.2 million doses of Vero Cell vaccine purchased through the COVAX's cost-sharing mechanism. (spotlightnepal.com)
  • A cargo flight of China South Airlines carrying 1.2 million doses of Vero Cell vaccine has landed at Tribhuvan International Airport on Tuesday morning," said Badebabu Thapa, a senior official at the Logistic Management Section under the Department of Health Services. (spotlightnepal.com)
  • Nepal received 1.2 million doses of Vero Cell vaccination under the COVAX cost-sharing mechanism on Wednesday. (tyrocity.com)
  • Vero cells are a lineage of cells used in cell cultures. (wikipedia.org)
  • The evaluation of SPRP as a supplement showed that there was no statistical difference in cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. (springer.com)
  • Therefore, in Vero cell cultures, the results suggest that SPRP and CPRP can be used as a supplement and scaffold, respectively. (springer.com)
  • The finding of IBV mRNA in the cell cultures demonstrates that the CRG-BETA IBV strain is replicating in the VERO cells and regarding S1 sequence analysis, the lack of nucleotide mutations shows that CRG-BETA might have reached a fixed status. (usp.br)
  • In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. (biomedcentral.com)
  • Protein demand and, thus, the demand for cell cultures in protein production fluctuate. (biomedcentral.com)
  • This would allow a more flexible handling of cell cultures adapted to the demand, e.g. a rapid upscaling of cultures after storage, i.e. keeping cells in "stand-by" storage. (biomedcentral.com)
  • In the present study, we investigated the anti-DENV activity of insect cell-derived anionic septapeptides from C6/36 mosquito cell cultures persistently infected with DENV. (unboundmedicine.com)
  • Cultured eukaryote cells such as human foreskin fibroblasts or vero cell cultures are used to inoculate the specimen. (medscape.com)
  • Early in infection, the surfaces of infected cells are embossed by scattered groups of twisted strands, and diffuse patches of label for viral antigens cover regions marked by these strands. (rupress.org)
  • VSV infection triggered rapid differentiation of blood monocytes into immature dendritic cells as well as their apoptosis, which depended on caspase 3/7 activation. (karger.com)
  • Monocyte differentiation required infectious VSV, but loss of CD14+ cells was also associated with the presence of a cytokine/chemokine milieu produced in response to VSV infection. (karger.com)
  • The in vitro growth characteristics of these two virus lines in white blood cells (predominantly myeloblasts) from a patient with acute myeloid leukemia were measured, starting with low multiplicities of infection. (karger.com)
  • These molecules were previously shown to protect C6/36 and Vero cells against DENV infection. (unboundmedicine.com)
  • A cell culture positive for herpes simplex virus (HSV) implies probable active infection. (medscape.com)
  • A negative HSV cell culture result does not rule out HSV infection, particularly if the specimen is from cerebrospinal fluid (CSF) or nonvesicular lesions. (medscape.com)
  • One of these compounds (ebselen) also exhibited promising antiviral activity in cell-based assays. (nature.com)
  • Vero E6 cells were used for cell culture experiments, and neutralization assays were performed. (news-medical.net)
  • A CELL LINE derived from the kidney of the African green (vervet) monkey, (CHLOROCEBUS AETHIOPS) used primarily in virus replication studies and plaque assays. (bvsalud.org)
  • Vero cells productively infected with the Halle strain of measles virus have been studied by means of surface replication, freeze-fracturing, and surface labeling with horseradish peroxidase-measles antibody conjugate in order to examine changes in the structure of the cell membrane during viral maturation. (rupress.org)
  • Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. (abeomics.com)
  • Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. (abeomics.com)
  • The morphological analysis, carried out on the third and fifth days, did not verify changes in the typical morphology of Vero cells cultured with SPRP. (springer.com)
  • Homozygous deletion of this distal Sox2 control region (SCR) caused significant reduction in Sox2 mRNA and protein levels, loss of ES cell colony morphology, genome-wide changes in gene expression, and impaired neuroectodermal formation upon spontaneous differentiation to embryoid bodies," wrote the investigators. (genengnews.com)
  • Strain CRG-BETA, propagated in chicken embryos, was inoculated in VERO cells monolayers up to the 4th passage and each passage was monitored with an RT-PCR targeted to the S1 gene (nt 705to 1094) and an RT-PCR to the protein 5a mRNA. (usp.br)
  • Two cell mouse embryos (NMRI Strain) were flushed from the excised uteri of superovulated mice. (celljournal.org)
  • A strain of Vesicular Stomatitis virus, serotype Indiana (VSV), was compared after a single in vitro passage and after 16 in vitro passages in human white blood cells from patients suffering mostly from acute myeloid leukemia. (karger.com)
  • Infectivity titrations in chick embryo cells gave consistently about ten times higher values than the same titrations done in human diploid cells, strain Wi38. (karger.com)
  • The COVID-19 Vaccine (Vero Cell), Inactivated is made from the SARS-CoV-2, 19nCoV-CDC-Tan-HB02 strain which is inoculated on the Vero cells for culturing, harvesting, -propiolactone-inactivation, concentration and purification, then followed by adsorption with aluminium hydroxide adjuvant to form the liquid vaccine. (who.int)
  • ACAM2000 is produced in Vero cells and derived from a clonal isolate of Dryvax, the New York City Board of Health strain widely used during the smallpox eradication campaign [ 1-5 ]. (cdc.gov)
  • The 'Vero' lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp. (wikipedia.org)
  • The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus. (wikipedia.org)
  • The Vero cell lineage is continuous and aneuploid, meaning that it has an abnormal number of chromosomes. (wikipedia.org)
  • The researchers discovered that this region is required to both turn Sox2 on, and for the embryonic stem cells to maintain their characteristic appearance and ability to differentiate into all the cell types of the adult organism. (genengnews.com)
  • Fig-1: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/VERO stable cell line. (abeomics.com)
  • Vero E6 cells show some contact inhibition, so are suitable for propagating viruses that replicate slowly. (wikipedia.org)
  • T8300These cells may exhibit some degree of contact inhibition after forming a monolayer and is therefore is useful?for supporting the growth of slowly. (topsan.org)
  • The purpose of this study was to investigate the effect of RPMI and T6 medium on two cell mouse embryo co-culture with Vero cells. (celljournal.org)
  • The second control and experimental groups cultured on RPMI only and Vero using RPMI media respectively. (celljournal.org)
  • Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells, surprisingly, strongly enhanced cold-induced cell injury in these cells in comparison to cold storage in Krebs-Henseleit buffer or other cell culture media (DMEM, L-15 and M199). (biomedcentral.com)
  • i.e. concentrations as in RPMI 1640) evoked a cell injury and loss of metabolic function corresponding to that observed in RPMI 1640. (biomedcentral.com)
  • Deferoxamine improved cell survival and preserved metabolic function in modified Krebs-Henseleit buffer as well as in RPMI 1640. (biomedcentral.com)
  • Here, we use primary nasal epithelial cells (NECs) from children and adults, differentiated at an air-liquid interface to show that the ancestral SARS-CoV-2 replicates to significantly lower titers in the NECs of children compared to those of adults. (plos.org)
  • Its main pathogenic property is the production of Shiga toxin (Stx), which inhibits the protein synthesis of host cells leading to cell death ( 3 , 4 ). (cdc.gov)
  • In the past few years, the SARS-Cov-2 Vaccine (Vero Cell) market experienced a huge change under the influence of COVID-19 and Russia-Ukraine War, the global market size of SARS-Cov-2 Vaccine (Vero Cell) reached XXX million $ in 2022 from XXX in 2017 with a CAGR of xxx from 2017-2022. (reportsweb.com)
  • Publisher predicts that the global SARS-Cov-2 Vaccine (Vero Cell) market size will reach XXX million $in 2028 with a CAGR of xx% from 2022-2028. (reportsweb.com)
  • The government has started administrating second dose of Vero Cell vaccine in Kathmandu from Friday. (nepalpress.com)
  • The results suggest that the embryo co-culture with Vero cells improve the in vitro development, but the type of the medium must be considered, which can be helpful for cell proliferation and embryos development. (celljournal.org)
  • Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G. Serum-free cell culture: the serum-free media interactive online database. (springer.com)
  • Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular ardiomyoplasty. (springer.com)
  • The government said newborn calf serum is used only for preparation and growth of vero cells and different kinds of bovine and other animal serum are standard enrichment ingredient used globally for vero cell growth. (tribuneindia.com)
  • These vero cells, after the growth, are washed with water, with chemicals (also technically known as buffer), many times to make it free from the newborn calf serum. (tribuneindia.com)
  • Research strains transfected with viral genes: Vero F6 is a cell transfected with the gene encoding HHV-1 entry protein glycoprotein-H (gH). (wikipedia.org)
  • At this stage, label for viral antigens on the surface of the cell membrane is organized into stripes lying on the crests of strands. (rupress.org)
  • The fractured membranes of viral buds are continuous sheets of these small particles, and the spacing between both nucleocapsids and stripes of surface antigen in buds is less than in the surrounding cell membrane. (rupress.org)
  • Thus, surface antigens, membrane particles, and nucleocapsids attached to the cell membrane are mobile within the plane of the membrane during viral maturation. (rupress.org)
  • These interactions reveal that the viral genome and subgenomes adopt alternative topologies inside cells and engage in different interactions with host RNAs. (nih.gov)
  • Then these vero cells are infected with corona virus for viral growth. (tribuneindia.com)
  • The vero cells are completely destroyed in the process of viral growth. (tribuneindia.com)
  • Chromosome 12 of Vero cells has a homozygous ~9-Mb deletion, causing the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitors CDKN2A and CDKN2B in the genome. (wikipedia.org)
  • Similar Ca 2+ and phosphate concentrations did not increase cold-induced cell injury in the kidney cell line LLC-PK 1 , porcine aortic endothelial cells or rat hepatocytes. (biomedcentral.com)
  • However, more extreme conditions (Ca 2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. (biomedcentral.com)
  • These data suggest that the combination of low calcium and high phosphate concentrations in the presence of glucose enhances cold-induced, iron-dependent injury drastically in Vero-B4 cells, and that a tendency for this pathomechanism also exists in other cell types. (biomedcentral.com)
  • The original cell line was named Vero after an abbreviation of verda reno, which means 'green kidney' in Esperanto, while vero itself means 'truth' in Esperanto. (wikipedia.org)
  • The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014. (wikipedia.org)
  • Vero E6, also known as Vero C1008 (ATCC No. CRL-1586) This line is a clone from Vero 76. (wikipedia.org)
  • The Vero cell line was initiated from the kidney of a normal adult African Green monkey. (topsan.org)
  • T8298This Vero cell line has been adapted for growth in animal component free medium. (topsan.org)
  • or (4) resale of the cell line whether or not such cell lines are resold for use in research. (abeomics.com)
  • The cell lines in our cell bank have been successfully used in gene-editing cell line generation, applicable for all kinds of gene-editing experiments. (ubigene.us)
  • We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. (biomedcentral.com)
  • Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. (sigmaaldrich.com)
  • Virus release was evaluated in a murine cell line (L929). (karger.com)
  • Together, these data identify a distal control region essential for Sox2 transcription in ES cells. (genengnews.com)
  • ACE2/VERO cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein (Abeomics, Cat. (abeomics.com)
  • Dr Gautam said that those people who missed out on their booster dose of the Vero Cell vaccine on previous vaccination campaigns can take their second dose vaccine during the 2nd dose vaccination campaign. (risingnepaldaily.com)
  • Students above 14 years of age and people above the age of 40 years, who have completed 21 days after receiving the first dose, will be administered with second dose of Vero Cell vaccine. (nepalpress.com)
  • All cells of human and murine origin in our cell bank have available STR Authentication reports, guaranteeing accurate cell identity. (ubigene.us)
  • More than 1,000 infectious units of VSV (as measured in chick embryo cells) were produced per myeloblast within 44 h. (karger.com)
  • Vero F6 was transfected via a concatenated plasmid with the gH gene after a copy of the HHV-1 glycoprotein-D (gD) promoter region. (wikipedia.org)
  • University of Toronto (U of T) researchers investigating stem cells in mice report for the first time an instance of a relationship between the Sox2 gene , which is critical for early development, and a region elsewhere on the genome that effectively regulates its activity. (genengnews.com)
  • The discovery could mean a significant advance in human regenerative medicine, as the Sox2 gene is essential for maintaining embryonic stem cells that can develop into any cell type of a mature animal. (genengnews.com)
  • We studied how the Sox2 gene is turned on in mice, and found the region of the genome that is needed to turn the gene on in embryonic stem cells," said Jennifer Mitchell, Ph.D., of U of T's department of cell and systems biology. (genengnews.com)
  • Like the gene itself, this region of the genome enables these stem cells to maintain their ability to become any type of cell, a property known as pluripotency. (genengnews.com)
  • It was previously thought that regions much closer to the Sox2 gene were the ones that turned it on in embryonic stem cells. (genengnews.com)
  • To contact the gene, the DNA makes a loop that brings the SCR close to the gene itself only in embryonic stem cells. (genengnews.com)
  • It is possible that the formation of the loop needed to make contact with the Sox2 gene is an important final step in the process by which researchers practicing regenerative medicine can generate pluripotent cells from adult cells. (genengnews.com)
  • Nina Bauer, associate director of autologous cell therapy commercial development, Lonza Pharma and Biotech Lonza Emerging Technologies is focusing on cell and gene therapies. (bioprocessintl.com)
  • ACE2 is a type I transmembrane metalloenzyme located on the outer surface of endothelial cells in the lung, arteries, heart, kidney and intestines. (abeomics.com)
  • as host cells for eukaryotic parasites, specially of the trypanosomatids Vero (ATCC No. CCL-81) Isolated from C. aethiops kidney on 27 Mar 1962 Vero 76 (ATCC No. CRL-1587) Isolated from Vero in 1968, it grows to a lower saturation density (cells per unit area) than the original Vero. (wikipedia.org)
  • No differences were seen in the following parameters: Length of latency phase, growth rate, final titer, thermal inactivation rate, plaque size, titer difference in chick embryo vs. human diploid cells, and extent of neutralization by a standard rabbit antiserum. (karger.com)
  • Title : Japanese Encephalitis (JE) vaccine = GRADE for inactivated Vero cell culture- derived JE vaccine (JE-VC) Personal Author(s) : Walter, Emmanuel B.;Hills, Susan L. (cdc.gov)
  • When culturing human diploid cell lines , the cell viability and amplification effect are significantly better than those of traditional microcarriers. (cytoniche.com)
  • Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. (springer.com)
  • Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly (lactic acid) substrates. (springer.com)
  • The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. (springer.com)
  • Cell culture process parameters such as temperature, dissolved oxygen, pH, rotating speed, Air, O2, CO2 and N2 can be controlled with high precision and automation, providing a good environment for cells and promoting cell proliferation. (cytoniche.com)
  • Combined with mature automatic medium exchange, perfusion and other processes, high-density cell proliferation can be achieved within limited time and space. (cytoniche.com)
  • Dr. Mitchell and her colleagues eliminated this possibility when they deleted these nearby regions in the genome of mice and found there was no impact on the gene's ability to be turned on in embryonic stem cells. (genengnews.com)
  • Fundamental Techniques in Cell Culture: a Laboratory Handbook. (wikipedia.org)
  • Tissue engineering performs the culture of cells on scaffolds, aiming at the restoration of damaged tissue. (springer.com)
  • For the cell culture into the scaffold, it is necessary to establish certain conditions, such as the type of supplementation of the culture medium. (springer.com)
  • Therefore, the aim of this research was to evaluate the platelet-rich plasma (PRP) as a supplement and scaffold for Vero cell culture, in a one case report study. (springer.com)
  • It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 o C water-bath, transfer to a tube containing 10 ml of growth medium without Hygromycin, spin down cells, resuspend cells in pre-warmed growth medium without Hygromycin, transfer resuspended cells to T25 flask and culture in 37 o C-CO 2 incubator. (abeomics.com)
  • To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. (abeomics.com)
  • The bioreactor and microcarrier suspension culture technology enable large-scale cell culture , which has been widely used in vaccine production. (cytoniche.com)
  • The online live cell counter can monitor the number of viable cells within the system in real time during the culture process (Figure 3). (cytoniche.com)
  • The first experimental group was co-culture with Vero using T6 media. (celljournal.org)
  • Inactivated Vero cell culture-derived JE vaccine (Ixiaro [JE-VC]) is the only JE vaccine that is licensed and available in the United States. (cdc.gov)
  • The criterion standard for HSV laboratory testing is the cell culture. (medscape.com)
  • She is also the lead investigator of a study ("A Sox2 distal enhancer cluster regulates embryonic stem cell differentiation potential") published in Genes & Development . (genengnews.com)
  • Ehrenfest DMD, Del Corso M, Diss A, Mouhyi J, Charrier J-B. Three-dimensional architecture and cell composition of a Choukroun's platelet-rich fibrin clot and membrane. (springer.com)
  • Tissue, cells or virus corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein S1. (abcam.com)
  • This inhibitory effect was likely mediated through various routes including the increased production of antiviral cytokines (IFN-I), activation of mononuclear cell migration, and upregulation of the expression of antiviral miRNAs (has-miR-30e*, has-miR-133a, and has-miR-223) and inflammation-related miRNAs (has-miR-146a and has-miR-147). (unboundmedicine.com)
  • To achieve satisfactory results, cells should not be passaged over 16 times. (abeomics.com)
  • compared with traditional microcarrier, it has obvious advantages in cell expansion and virus production. (cytoniche.com)
  • Unlike other DNA viruses, the variola virus multiplies in the cytoplasm of parasitized host cells. (medscape.com)
  • Lassa fe- Lassa virus in many more districts and states in en- ver is endemic in West Africa and has been reported demic countries of the West African sub-region and from Sierra Leone, Guinea, Liberia, and Nigeria4-7. (folkhalsomyndigheten.se)
  • These provide the proteins and growth factors needed to promote cell renewal and healing. (sikoramedical.com)
  • When used in combination with the 3D FloTrix® vivaSPIN automated bioreactor (Figure 2), it can achieve the stepwise scale-up of cells, and complete the harvesting of cells, viruses and cell products. (cytoniche.com)
  • AmCyan Live/Dead stain is used in all panels for identification of live cells. (jax.org)
  • A continuous cell lineage can be replicated through many cycles of division and not become senescent. (wikipedia.org)
  • As a recent example, CoronaVac, COVID-19 vaccine developed by Sinovac Biotech uses vero cells in production and "Vero" term can be seen on the vaccine container. (wikipedia.org)
  • This cold-induced cell injury has been shown to be mediated by reactive oxygen species (ROS) formed in an iron-dependent way [ 10 - 13 ] in most cell types. (biomedcentral.com)
  • Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)