ThapsigarginHeLaStreptomycesGlycosylationInhibitionHomologuesHomologousIntermediatesSynthesisEnhancesDolicholAntibioticsGlycoproteinTreatmentVitroCellsFormationEffect of tunicamycinPresence of tunicamycinAntibioticGlycosylation inhibitorInhibitorInhibitsStressSecretionProteinProteinsDataEffectsCell deathStudyExpression
Thapsigargin4
- The treatments were done with ER stress inducers thapsigargin and tunicamycin, and with IRE1 inhibitors KIRA6 and 4μ8c, or the combination of those. (helsinki.fi)
- The in vitro effects of nicotine and selected antibiotics, tunicamycin and thapsigargin on human Breast carcinoma (mcf-7) cells. (uwc.ac.za)
- Whole cell extracts (30 µg lysate) of K-562 (Lane 1), HeLa (Lane 2), HeLa treated with Tunicamycin (Lane 3), A549 (Lane 4), A549 treated with Thapsigargin (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). (thermofisher.com)
- We further compare the effects of tunicamycin (5 mu g/ml) to those of thapsigargin (1 mu M) and DTT (2 mM), both activating the UPR through different mechanisms. (torvergata.it)
HeLa2
- Tunicamycin has been used to study the effect of N-linked glycosylation of human proton-coupled folate transporter (HsPCFT) in HeLa cells. (sigmaaldrich.com)
- Here, we employ stable isotope labeling with amino acids in cell culture (SILAC)-based proteomics to quantify the response of over 6200 proteins to increasing concentrations of tunicamycin in HeLa cells. (torvergata.it)
Streptomyces4
- Tunicamycin is produced by several bacteria, including Streptomyces clavuligerus and Streptomyces lysosuperificus. (wikipedia.org)
- Tunicamycin Ready Made Solution 5mg/mL DMSO, Streptomyces sp. (sigmaaldrich.com)
- Tunicamycin from Streptomyces sp. (sigmaaldrich.com)
- Tunicamycin was first isolated from Streptomyces lysosuperificus based on its antiviral activity. (sigmaaldrich.com)
Glycosylation5
- Tunicamycin blocks N-linked glycosylation (N-glycans) and treatment of cultured human cells with tunicamycin causes cell cycle arrest in G1 phase. (wikipedia.org)
- Tunicamycin has also been used to study the functional effects of coxsackievirus and adenovirus receptor (CAR) glycosylation in COS-7 cells. (sigmaaldrich.com)
- Dr. Perez-Soler has published over a hundred articles, and papers including, "Activation of ER stress and inhibition of EGFR N-glycosylation by tunicamycin enhances susceptibility of human non-small cell lung cancer cells to erlotinib," published in the Cancer Chemother Pharmacol in January of 2009. (mesothelioma.com)
- Tunicamycin increases desensitization of junctional and extrajunctional acetylcholine receptors expressed in Xenopus oocytes by a mechanism independent of N-glycosylation blocking. (aspetjournals.org)
- Baculovirus replication: glycosylation of polypeptides synthesized in Trichoplusia ni nuclear polyhedrosis virus-infected cells and the effect of tunicamycin. (microbiologyresearch.org)
Inhibition1
- Modes of action of tunicamycin, liposidomycin B, and mureidomycin A: inhibition of phospho-N-acetylmuramyl-pentapeptide translocase from Escherichia coli . (warwick.ac.uk)
Homologues2
- Tunicamycin homologues have varying molecular weights owing to the variability in fatty acid side chain conjugates. (wikipedia.org)
- Tunicamycin Ready Made Solution contains homologues A,B,C, and D. The composition may vary from lot to lot. (sigmaaldrich.com)
Homologous2
- Tunicamycin is a mixture of homologous nucleoside antibiotics that inhibits the UDP-HexNAc: polyprenol-P HexNAc-1-P family of enzymes. (wikipedia.org)
- The resultant amino polyol is a versatile intermediate for the synthesis of any of the homologous tunicamycin antibiotics. (caltech.edu)
Intermediates1
- Tunicamycin inhibits bacterial and eukaryote N-acetylglucosamine transferases and prevents formation of N-acetylglucosamine lipid intermediates. (sigmaaldrich.com)
Synthesis1
- A concise synthetic route to the tunicamycin antibiotics is described, illustrated by the preparation of (+)-tunicamycin-V (1-V), Key features of the synthesis include (1) the development and application of a silicon-mediated reductive coupling of aldehydes and allylic alcohols to construct the undecose core of the natural product and (2) the development of an efficient procedure for the synthesis of the trehalose glycosidic bond within the antibiotic. (caltech.edu)
Enhances1
- In human cell lines, tunicamycin enhances cytotoxicity stimulated by cisplatin. (sigmaaldrich.com)
Dolichol1
- Tunicamycin blocks the formation of protein N-glycosidic linkages by inhibiting the transfer of N-acetylglucosamine 1-phosphate to dolichol monophosphate. (sigmaaldrich.com)
Antibiotics2
- Synthetic Studies of the Tunicamycin Antibiotics. (caltech.edu)
- A convergent synthetic route to the tunicamycin antibiotics. (caltech.edu)
Glycoprotein1
- Tunicamycin treatment experiments, biochemical fractionation and extraction experiments, and proteinase K protection experiments collectively indicate that Axl2p is an integral membrane glycoprotein at the plasma membrane. (embl.de)
Treatment2
- Both cell lines were used to test the effect of neurotrophic factors CDNF, MANF, and MANF mutant isomers on the UPR with and without tunicamycin treatment. (helsinki.fi)
- Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. (thermofisher.com)
Vitro1
- Tunicamycin plays an important role in the antitumor activity of trastuzumab on breast cancer in vitro and in vivo . (sigmaaldrich.com)
Cells1
- A similar assembly of desmosomal antigens, illustrated by immunofluorescence, could be demonstrated in MDCK cells treated with tunicamycin in low calcium medium. (soton.ac.uk)
Formation1
- 0.1mM), were treated with tunicamycin desmosome formation was triggered, as shown by immunofluorescence and electronmicroscopy. (soton.ac.uk)
Effect of tunicamycin1
- 9. Effect of Tunicamycin on the Biogenesis of Hepatitis C Virus Glycoproteins . (nih.gov)
Presence of tunicamycin3
- Unlike envelope glycoproteins of other viruses, the transmembrane glycoprotein El is glycosylated normally in the presence of tunicamycin. (nih.gov)
- Synthesis of the peplomeric glycoprotein E2 is not readily detectable in the presence of tunicamycin. (nih.gov)
- Disappearance of insulin receptors occurred more rapidly in the presence of tunicamycin than when protein synthesis was inhibited by cycloheximide and was accompanied by a diminution in sensitivity of the adipocytes to the acute effects of insulin and anit‐insulin receptor antibody on hexose uptake and metabolism. (elsevierpure.com)
Antibiotic3
- Tunicamycin, an antibiotic that inhibits protein glycosylation, elicited a rapid depletion of insulin binding activity at the surface of 3T3‐L1 adipocytes. (elsevierpure.com)
- Here we describe the crystal structure of the promising bacterial target phospho-N-acetylmuramoyl-pentapeptide translocase (MraY) in complex with the nucleoside antibiotic tunicamycin. (x-probe.org)
- Tunicamycin, an antibiotic produced by Streptomyces lysosuperificus, prevents the glycosylation of glycoproteins and can interfere with the secretion of thes. (irg-wp.com)
Glycosylation inhibitor2
- To assess whether underglycosylation of Oatp1 in yeast suppressed functional activity, Oatp1 was expressed in Xenopus laevis oocytes with and without tunicamycin, a glycosylation inhibitor. (nih.gov)
- These atypical glycosites were verified by the presence of glycans on their intact glycopeptides and further confirmed by specific inhibition of cells with an N-linked glycosylation inhibitor, tunicamycin. (nih.gov)
Inhibitor1
- Two populations of Leishmania donovani chagasi promastigotes resistant to the lethal effects of tunicamycin (TM), an inhibitor of N-linked glycosylation, were raised. (aai.org)
Inhibits1
- Tunicamycin is a mixture of homologous nucleoside antibiotics that inhibits the UDP-HexNAc: polyprenol-P HexNAc-1-P family of enzymes. (wikipedia.org)
Stress4
- We found that a double mutant of Arabidopsis IRE1A and IRE1B ( ire1a/ire1b ) is more sensitive to the ER stress inducer tunicamycin than the wild-type. (nature.com)
- Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. (thermofisher.com)
- The total number of ER-mitochondria associations detected by this approach increases in response to tunicamycin-induced ER stress, serum deprivation or reduced levels of mitofusin 2 (MFN2). (ed.ac.uk)
- To illustrate the benefits of a multiparametric approach, which obviates the need for normalization, we conducted a comprehensive islet toxicity study on the effects of the ER-stress-inducing agent Tunicamycin, performed by measuring multiple multiplexable endpoints. (insphero.com)
Secretion1
- We observed that sub-toxic concentrations of Tunicamycin did not alter ATP levels and had a negative impact not only on the insulin content but also on insulin secretion in response to acute glucose (16.7 mM) stimulation. (insphero.com)
Protein1
- Moreover, SHED inhibited the tunicamycin-induced up-regulated expression of BiP mRNA and protein in vitro. (epain.org)
Proteins1
- DIDS and DCPIB program considerably suppressed tunicamycin-induced upregulation of chaperone proteins GRP78 as well as the transcriptional aspect ATF4 (Statistics 2a and c), and downregulation of XBP1S (Amount 2b). (molecularcircuit.com)
Data1
- Tm, and ATF4 reached their top after 100?ng/ml tunicamycin publicity for 24?h (data not shown). (molecularcircuit.com)
Effects2
- Tunicamycin has different effects on the glycosylation of the two envelope glycoproteins of mouse hepatitis virus (MHV), a coronavirus. (nih.gov)
- This assay was followed by a multiparametric assessment of β-cell function that provided even more insight into the toxic effects of Tunicamycin. (insphero.com)
Cell death1
- 9. Imipramine protects mouse hippocampus against tunicamycin-induced cell death. (nih.gov)
Study1
- Tunicamycin is used as tool in the study of microbial biosynthetic mechanisms. (reference.md)
Expression1
- BiP expression was observed in PC12 cells induced by tunicamycin. (epain.org)