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  • link
  • Andreas Villunger and colleagues discuss the biology of the PIDDosome multiprotein complex and recent advances that link PIDDosome-dependent CASP2 activation to p53 activation in response to extra centrosomes. (biologists.org)
  • molecular
  • This structure revolutionises existing concepts of p53's molecular organisation and resolves conflicting data relating to its biochemical properties. (whiterose.ac.uk)
  • With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)
  • Molecular signatures associated with tumor aggressiveness as well as with disease progression and their relation to differences in signaling pathways implicated in gliomagenesis have recently been described. (mdpi.com)
  • The molecular tweezers, but not the clips, efficiently inhibit the formation of toxic oligomers and aggregates by amyloidogenic proteins associated with different diseases. (wikipedia.org)
  • Importantly, the molecular tweezers have been found to be effective and safe not only in the test tube but also in animal models of different diseases, suggesting that they may be developed as drugs against diseases caused by abnormal protein aggregation, all of which currently have no cure. (wikipedia.org)
  • Metallothionein (MT) is a family of cysteine-rich, low molecular weight (MW ranging from 500 to 14000 Da) proteins. (wikipedia.org)
  • The protein has a molecular weight of approximately 16,000, contains a single copper atom, is intensively blue, and has a fluorescence emission band centered at 308 nm. (wikipedia.org)
  • Azurin from P aeruginosa is a type I blue copper protein with a molecular mass of 14 kDa, while cytochrome c551 (9 kDa) is a haem-containing cytochrome. (wikipedia.org)
  • kinase
  • Proliferative-Enriched for neural stem cell markers, PTEN loss, EGFR amplified or normal, Akt (protein kinase B) cell signaling pathway activation, shorter survival than proneural subgroup. (mdpi.com)
  • Aurora kinase A also known as serine/threonine-protein kinase 6 is an enzyme that in humans is encoded by the AURKA gene. (wikipedia.org)
  • The SDT domain of the MDC1 protein is phosphorylated by caseine kinase 2 (CK2) which allows it to bind another MRN complex, the MDC1 protein can sense the DNA damage by binding to the γH2AX flag through its BRCT domain and brings the bound MRN complex to the site of damaged DNA and it facilitates the recruitment and retention of another ATM kinase. (wikipedia.org)
  • An additional study showed that beta-adrenergic signaling via protein kinase A and CaM kinase induces the expression of CARP, and that CARP may have a negative effect on contractile function. (wikipedia.org)
  • mutations
  • This thesis presents the first report of the comprehensive and quantitative analysis of the effects of tumor-derived mutations on the tetrameric structure of tumor suppressor protein p53, which plays a central role in maintaining genomic integrity. (springer.com)
  • Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs. (wikipedia.org)
  • PUMA can also function as an indicator of p53 mutations. (wikipedia.org)
  • Many cancers exhibit mutations in the p53 gene, but this mutation can only be detected through extensive DNA sequencing. (wikipedia.org)
  • Studies have shown that cells with p53 mutations have significantly lower levels of PUMA, making it a good candidate for a protein marker of p53 mutations, providing a simpler method for testing for p53 mutations. (wikipedia.org)
  • The pRb-binding motif and negatively charged region match to a segment of SV40 TAg beginning at residue 102 and ending at residue 115 as shown below: - Asn - Leu - Phe - Cys - Ser - Glu - Glu - Met - Pro - Ser - Ser - Asp - Asp - Glu - Functional studies of TAg proteins bearing mutations within this segment (amino acid positions 106 to 114, inclusive) demonstrate that certain deleterious mutations abolish malignant transforming activity. (wikipedia.org)
  • Deleterious mutations within this segment (amino acid positions 105 to 114, inclusive) also impair binding of the mutant TAg protein species to pRb, implying a correlation between transforming activity and the ability of TAg to bind pRb. (wikipedia.org)
  • Caspase
  • These results indicate that the increased expression and activation of p53, and the nitric oxide and caspase systems related to p53 may contribute to the development of antinociceptive tolerance to morphine in the mouse spinal cord. (diva-portal.org)
  • mRNA
  • In cardiomyocytes treated with doxorubicin, a model of anthracycline-induced cardiomyopathy, CARP mRNA and protein levels were depleted, myofilament gene transcription was attenuated and sarcomeres showed significant disarray. (wikipedia.org)
  • cytochrome
  • Azurin is a bacterial blue copper protein found in Pseudomonas, Bordetella, or Alcaligenes bacteria, which undergoes oxidation-reduction between Cu(I) and Cu(II), and transfers single electrons between enzymes associated with the cytochrome chain. (wikipedia.org)
  • Azurin possesses a relatively large hydrophobic patch close to the active site, and two residues in this hydrophobic patch, Met-44 and Met-64, are believed to be involved in its interaction with the redox partners cytochrome c551 and nitrite reductase Plastocyanin family of copper-binding proteins De Rienzo F, Gabdoulline RR, Menziani MC, Wade RC (2000). (wikipedia.org)
  • overexpression
  • 2. Starzynska T, Bromley M, Ghosh A, Stern PL. Prognostic significance of p53 overexpression in gastric and colorectal carcinoma. (labcorp.com)
  • replication
  • Protein p53 is an important checkpoint in the cell cycle, ensuring the integrity of DNA replication. (sciencephoto.com)
  • Other proteins that function to restrict HIV replication include TRIM5alpha and APOBEC3G. (wikipedia.org)
  • TRIM22 appears to prevent the movement of the HIV Gag protein to the plasma membrane and hence TRIM22 can block HIV replication in cell cultures by preventing the assembly of the virus. (wikipedia.org)
  • peptides
  • Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
  • adaptor
  • The MDC1s role in DDR is to function both as a mediator/adaptor protein mediating a complex of other DDR proteins at the site of DNA damage and repairing DNA damage through its PST domain. (wikipedia.org)
  • Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. (wikipedia.org)
  • chemotherapy
  • RATIONALE: Drugs used in chemotherapy such as soblidotin use different ways to stop tumor cells from dividing so they stop growing or die. (bioportfolio.com)
  • mammalian
  • Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
  • In mammalian cells the DRR is a network of pathways made up of proteins that function as either kinases, or and mediator/adaptors that recruit the kinases to their phosphorylation targets, these factors work together to detect DNA damage, and signal the repair mechanism as well as activating cell cycle checkpoints. (wikipedia.org)
  • phosphorylation
  • PUMA protein is degraded in a proteasome dependent manner and its degradation is regulated by phosphorylation at a conserved serine residue at position 10. (wikipedia.org)
  • enzymes
  • RATIONALE: Sorafenib may stop the growth of tumor cells by blocking some of the enzymes needed for cell growth. (bioportfolio.com)
  • Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. (wikipedia.org)
  • mutant
  • Furthermore, functional control of p53 via tetramer formation was demonstrated, based on the structure-function analysis of mutant p53. (springer.com)
  • sequences
  • p53 binding correlates with inverted repeat sequences, such as the ones that help form cruciform DNA structures. (wikipedia.org)
  • tetramer
  • Using ATP-stabilised p53, we have employed cryoelectron microscopy and single particle analysis to solve the first three-dimensional structure of the full-length p53 tetramer (resolution 13.7 Å). (whiterose.ac.uk)
  • The p53 molecule is a D2 tetramer, resembling a hollow skewed cube with node-like vertices of two sizes. (whiterose.ac.uk)
  • expression
  • Moreover, p53 expression in the spinal cord had increased significantly 14h after the last morphine administration. (diva-portal.org)
  • This protein localizes to the cytoplasm and its expression is induced by interferon. (wikipedia.org)
  • Under normal conditions (i.e., undetectable p53 expression), the AIMF2 gene is highly expressed in the heart, followed by liver and skeletal muscle, with low levels detected in the placenta, lung, kidney, and pancreas and the lowest in the brain. (wikipedia.org)
  • The expression of PUMA is regulated by the tumor suppressor p53. (wikipedia.org)
  • cells
  • Some block the ability of tumor cells to grow and spread. (bioportfolio.com)
  • Others find tumor cells and help kill them or deliver tumor-killing substances to them. (bioportfolio.com)
  • Inhibition or loss of MDC1 protein through studies with siRNA on human cells or knockout studies in mice have shown several defects at both the cellular and organismal level. (wikipedia.org)
  • pathways
  • The two pathways both activate initiator caspases, which then activate executioner caspases, which then kill the cell by degrading proteins indiscriminately. (wikipedia.org)
  • human
  • Human CARP is a 36.2kDa protein composed of 319 amino acids. (wikipedia.org)
  • Infection by most papillomavirus types, depending on the type, is either asymptomatic (e.g. most Beta-PVs) or causes small benign tumors, known as papillomas or warts (e.g. human papillomavirus1, HPV6 or HPV11). (wikipedia.org)
  • subgroup
  • 5. Visakorpi T, Kallioniemi OP, Heikkinen A, Koivula T, Isola J. Small subgroup of aggressive, highly proliferative prostatic carcinomas defined by p53 accumulation. (labcorp.com)
  • The PUMA protein is part of the BH3-only subgroup of Bcl-2 family proteins. (wikipedia.org)
  • activate
  • This protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. (wikipedia.org)
  • promoter
  • The protein down-regulates transcription from the HIV-1 long terminal repeat promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. (wikipedia.org)
  • p53 was found to be an upstream effector of CARP via upregulation of the proximal ANKRD1 promoter. (wikipedia.org)
  • LUNG CANCER
  • The study goal is to collect blood samples from individuals at risk for lung cancer in order to identify protein markers for diagnosis of lung cancer in the Chinese population. (bioportfolio.com)
  • activity
  • Animal studies have shown that PUMA does play a role in tumor suppression, but lack of PUMA activity alone does not translate to spontaneous formation of malignancies. (wikipedia.org)
  • The transforming activity of TAg is due in large part to its perturbation of the retinoblastoma (pRb) and p53 tumor suppressor proteins. (wikipedia.org)
  • function
  • Although there is evidence to suggest that Aurora C might be a chromosomal passenger protein, the cellular function of it is less clear. (wikipedia.org)
  • mice displayed normal cardiac function at baseline and in response to pressure overload-induced cardiac hypertrophy, suggesting that these proteins are not essential. (wikipedia.org)