• proliferation
  • The retention of abundant basement membrane molecules in the stromal space that includes pseudocystic cavities suggests that basement membrane matrix is essential for proliferation of adenoid cystic carcinoma cells. (nii.ac.jp)
  • Because rapid tumor proliferation is associated with poor patient prognosis, here we characterized the transcriptional signature of rapidly proliferating colorectal cancer cells in an attempt to identify novel candidate therapeutic targets. (aacrjournals.org)
  • Furthermore, in vivo and in vitro studies using Igf1r monoclonal antibodies ( 11-13 ), Igfr-1 inhibitors ( 14, 15 ), and antisense technology ( 16 ) have shown that Igfr-1 is functionally important for tumor cell growth and cancer cell proliferation in RMS. (aacrjournals.org)
  • The conversion of cell membrane arachidonic acid (20:4n-6) to omega-6 prostaglandin and omega-6 leukotriene eicosanoids during the inflammatory cascade provides many targets for pharmaceutical drugs to impede the inflammatory process in atherosclerosis, asthma, arthritis, vascular disease, thrombosis, immune-inflammatory processes, and tumor proliferation. (wikipedia.org)
  • A ratio of 2.5:1 reduced rectal cell proliferation in patients with colorectal cancer, whereas a ratio of 4:1 had no effect. (wikipedia.org)
  • Experiments show that INH1 potently inhibits the proliferation of multiple human breast cancer cell lines, cervical HeLa cells, and colon cancer cells in vitro. (wikipedia.org)
  • These formazan dyes are commonly used in cell proliferation and toxicity assays like the EpiDerm and EpiSkin tests since they only stain living, metabolically active cells. (wikipedia.org)
  • The role for the Wnt/β-catenin signaling in cancer has been well defined: β-catenin drives transcription of genes that contribute to the tumor phenotype by regulating processes such as proliferation, survival and invasion. (wikipedia.org)
  • The growth-inhibitory effects of SFRP1 were due primarily to decreased cell proliferation rather than an increase in apoptosis. (wikipedia.org)
  • malignant
  • Hypoxia was further demonstrated to cause a more malignant anchorage-independent growth phenotype in the resistant cells, which can be abolished by knocking down ABCG2. (hindawi.com)
  • Glucose depletion is often observed in malignant tumors due to insufficient blood supply in the core of solid tumors [ 5 ] and increased rate of glycolysis [ 6 ]. (hindawi.com)
  • Panobinostat inhibits multiple histone deacetylase enzymes, a mechanism leading to apoptosis of malignant cells via multiple pathways. (wikipedia.org)
  • As of August 2012[update], it is being tested against Hodgkin's Lymphoma, cutaneous T cell lymphoma (CTCL) and other types of malignant disease in Phase III clinical trials, against myelodysplastic syndromes, breast cancer and prostate cancer in Phase II trials, and against chronic myelomonocytic leukemia (CMML) in a Phase I trial. (wikipedia.org)
  • Moreover, a recent study with various malignant tumor cells showed that celecoxib could inhibit the growth of these cells, even though some of these cancer cells didn't even contain COX-2. (wikipedia.org)
  • vitro
  • Cells grown in vitro from neoplastic tissue. (jove.com)
  • Overexpression of ABCG2 in cancer cell lines in vitro has been shown to confer MDR to a variety of anticancer drugs including mitoxantrone, irinotecan, methotrexate, flavopiridol, and anthracyclines [ 1 ]. (hindawi.com)
  • Panobinostat has also been found to significantly increase in vitro the survival of motor neuron (SMN) protein levels in cells of patients suffering from spinal muscular atrophy. (wikipedia.org)
  • A 2015 study suggested Panobinostat was effective in preventing diffuse intrinsic pontine glioma cell growth in vitro and in vivo, identifying it as a potential drug candidate. (wikipedia.org)
  • The publication of two seminal research papers in 1998 outlining the isolation of these cells provided an in vitro method by which researchers may characterise PaSCs in both health and pathology. (wikipedia.org)
  • VEGF
  • Moreover, endothelial cells lacking Bmx showed impaired phosphorylation of extracellular signal-regulated kinase (Erk) upon VEGF stimulation, indicating that Bmx contributes to the transduction of vascular endothelial growth factor signals. (aacrjournals.org)
  • pathways
  • Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
  • However, experience with RTK inhibitors shows that resistance and/or alternative signaling pathways can evolve in nearly a third of all tumors, thereby limiting efficacy of therapies targeting a single protein responsible for tumor maintenance and progression ( 19, 20 ). (aacrjournals.org)
  • Receptors on bone marrow cells can activate downstream signaling pathways in part via various receptor-associated cytoplasmic tyrosine kinases, including Bmx ( 4, 5 ). (aacrjournals.org)
  • inhibit
  • The Her2 inhibitor lapatinib cooperates with NVP-AEW541 to reduce Igf1r phosphorylation and to inhibit cell growth even though lapatinib alone has little effect on growth. (aacrjournals.org)
  • However, when the ability of all these compounds to kill tumor cells in cell culture was investigated, it turned out that the antitumor potency did not at all depend on whether or not the respective compound could inhibit COX-2, showing that inhibition of COX-2 was not required for the anticancer effects. (wikipedia.org)
  • One of these compounds, 2,5-dimethyl-celecoxib, which entirely lacks the ability to inhibit COX-2, actually turned out to display stronger anticancer activity than celecoxib itself and this anticancer effect could also be verified in highly drug-resistant tumor cells and in various animal tumor models. (wikipedia.org)
  • molecules
  • The pseudocysts are surrounded by tumor cells and are filled with extracellular matrix components such as basement membrane molecules. (nii.ac.jp)
  • We have demonstrated that these basement membrane molecules were produced by ACC2 and ACC3 cells, established independently from human salivary adenoid cystic carcinomas. (nii.ac.jp)
  • We have also shown that adenoid cystic carcinoma dells attach, spread, and grow rapidly on matrices conta … More ining basement membrane molecules, using ACC3 cells of human adenoid cystic carcinoma origin. (nii.ac.jp)
  • The results indicate that our collagen gel culture system provides physiological conditions for ACC2 cells to secrete particular extracelluar matrix molecules and form pseudocystic spaces. (nii.ac.jp)
  • Publications] Cheng, J.: 'Biosynthesis of basement membrane molecules by salivary adenoid cystic carcinoma cells : an immunofluorescence and confocal microscopic study. (nii.ac.jp)
  • Tumor microenvironment represents an attractive new drug target because it allows complex interaction between a tumor and its surrounding normal cells, molecules, and blood vessels, which all participate in tumor progression. (hindawi.com)
  • They can be thought of as inhabiting within a complex milieu of normal cells, blood vessels, endogenous small molecules, and secreted factors, which collectively comprise the tumor microenvironment. (hindawi.com)
  • determined that Saos-2 cells "possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules. (wikipedia.org)
  • An important class of molecules involved in morphogenesis are transcription factor proteins that determine the fate of cells by interacting with DNA. (wikipedia.org)
  • At the end of this cascade are classes of molecules that control cellular behaviors such as cell migration, or, more generally, their properties, such as cell adhesion or cell contractility. (wikipedia.org)
  • One of the ways this can occur is when cells share the same cell-to-cell adhesion molecules. (wikipedia.org)
  • For instance, homotypic cell adhesion can maintain boundaries between groups of cells that have different adhesion molecules. (wikipedia.org)
  • Gangliosides are present and concentrated on cell surfaces, with the two hydrocarbon chains of the ceramide moiety embedded in the plasma membrane and the oligosaccharides located on the extracellular surface, where they present points of recognition for extracellular molecules or surfaces of neighboring cells. (wikipedia.org)
  • protein
  • However, some tumor cells are able to repair this type of DNA damage, and therefore diminish the therapeutic efficacy of temozolomide, by expressing a protein O6-alkylguanine DNA alkyltransferase (AGT) encoded in humans by the O-6-methylguanine-DNA methyltransferase (MGMT) gene. (wikipedia.org)
  • mediates
  • Furthermore, these results highlight the importance of pathologically relevant tissue culture models to study the complex physical and chemical processes by which the cancer microenvironment mediates new vessel formation. (sigmaaldrich.com)
  • The activation of P2 receptors induces intracellular calcium signalling which mediates the fibrogenic function of activated stellate cells. (wikipedia.org)
  • pancreatic
  • Panobinostat has been found to synergistically act with sirolimus to kill pancreatic cancer cells in the laboratory in a Mayo Clinic study. (wikipedia.org)
  • In the study, investigators found that this combination destroyed up to 65 percent of cultured pancreatic tumor cells. (wikipedia.org)
  • The finding is significant because the three cell lines studied were all resistant to the effects of chemotherapy - as are many pancreatic tumors. (wikipedia.org)
  • Pancreatic stellate cells (PaSCs) are classified as myofibroblast-like cells that are located in exocrine regions of the pancreas. (wikipedia.org)
  • While the pathogenesis of fibrosis remains elusive, the activation of stellate cells contribute to pancreatic fibrosis. (wikipedia.org)
  • vivo
  • Prolonged culture resulted in a 10-fold decrease in the incidence of B16a lung colonies, whereas passage in vivo for 150 days did not affect lung colony formation by tumor cells isolated from enzymatic dispersates by centrifugal elutriation. (rti.org)
  • This" multicellular resistance" reflects the relative intrinsic drug-resistant phenotype of most solid tumors growing in vivo and is due to factors such as limited drug penetration or reduced fractions of proliferating cells. (aacrjournals.org)
  • Hence, this compound has the potential to circumvent multicellular drug resistance and, as such, may show promising activity against solid tumors with low growth fractions in vivo , which are frequently intrinsically resistant to conventional cytotoxic anticancer drugs. (aacrjournals.org)
  • The rationale is that if a drug demonstrates antitumor activity in a spheroid model or screen comparable to or greater than that seen in a conventional monolayer system, it could indicate more promising efficacy for the treatment of solid tumors in vivo . (aacrjournals.org)
  • lines
  • Vascular mimicry in cultured head and neck tumour cell lines. (biomedsearch.com)
  • We suggest this is common phenomenon in head and neck squamous cell carcinoma (HNSCC) cell lines and other aggressive tumour cell lines. (biomedsearch.com)
  • We present experimental evidence of vasculogenic mimicry in HNSCC cell lines and compare them with other tumours and a positive control vascular cell line. (biomedsearch.com)
  • Other tumour cell lines also displayed this property. (biomedsearch.com)
  • The focus of this study was to examine the CTC-iChip and determine if the device could properly capture CTCs to aid the formation of cell lines to represent the genetic status of all existing tumor sites. (oncologynurseadvisor.com)
  • Cell lines with longevity were successfully established from 6 patients. (oncologynurseadvisor.com)
  • Additional samples from 3 of these patients were used to set up new cell lines to track the effectiveness of therapy via tumor change. (oncologynurseadvisor.com)
  • Circulating tumor cells captured with a microchip-based device developed at the Massachusetts General Hospital (MGH) Center for Engineering in Medicine and the MGH Cancer Center can be cultured to establish cell lines for genetic analysis and drug testing. (oncologynurseadvisor.com)
  • Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
  • All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
  • Complementary studies using primary tumor samples and cell lines have shown similarly that the level of IGF signaling is increased in RMS ( 6, 7 ). (aacrjournals.org)
  • growth
  • RESULTS: There were observable differences in growth of the cells on laboratory plastic and collagen matrix. (biomedsearch.com)
  • It is also believed to be a survival factor for cancer stem cells to drive tumor growth. (hindawi.com)
  • This study investigated the upregulation of ABCG2 by these adverse growth conditions within the tumor microenvironment. (hindawi.com)
  • It may also contribute to the maintenance of stem or progenitor cells as a survival factor, ultimately driving tumor growth [ 4 ]. (hindawi.com)
  • In this study, we characterized the function of Bmx in tumor growth using both Bmx knockout and transgenic mice. (aacrjournals.org)
  • Although recent reports have suggested that Bmx is involved in prostate cancer and glioblastoma growth ( 14, 15 ), little is known about Bmx in tumor biology. (aacrjournals.org)
  • Here, we have used Bmx gene-deleted mice ( 6 ) and K14-Bmx transgenic mice ( 7 ) to explore the function of Bmx in tumor growth. (aacrjournals.org)
  • We found, with one exception, that PS-341 showed equal or greater activity in spheroids than in the respective monolayer cell cultures, even in a prostate cancer spheroid model with a very low growth fraction. (aacrjournals.org)
  • The biological effects of the omega-6 fatty acids are largely produced during and after physical activity for the purpose of promoting growth and during the inflammatory cascade to halt cell damage and promote cell repair by their conversion to omega-6 eicosanoids that bind to diverse receptors found in every tissue of the body. (wikipedia.org)
  • but studies in laboratory mice have shown that nelfinavir is able to suppress the growth of tumors in these animals, which represents a promising lead towards testing this drug in humans as well. (wikipedia.org)
  • In biological specimens containing a growth medium, appearance over time of a detectable and rising heat flow signal is a simple general indicator of the presence of some type of replicating cells. (wikipedia.org)
  • Growth in culture and anchorage-independent growth were inhibited in SFRP1-expressing UMRC3 cells. (wikipedia.org)
  • embryonic development
  • As of early 2016, there are three families belonging to the MACPF superfamily: 1.C.12 - The Thiol-activated Cholesterol-dependent Cytolysin (CDC) Family 1.C.39 - The Membrane Attack Complex/Perforin (MACPF) Family 1.C.97 - The Pleurotolysin Pore-forming (Pleurotolysin) Family Proteins containing MACPF domains play key roles in vertebrate immunity, embryonic development, and neural-cell migration. (wikipedia.org)
  • The process controls the organized spatial distribution of cells during the embryonic development of an organism. (wikipedia.org)
  • During embryonic development, cells are restricted to different layers due to differential affinities. (wikipedia.org)
  • human
  • The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
  • It has been shown that the deregulation of this critical developmental pathway occurs in several human tumor entities. (wikipedia.org)
  • lung
  • We first investigated the effects of cell source and culture conditions on lung colony formation by i.v. injected B16a (B16 amelanotic melanoma) cells and inhibition of tumor colony formation by the thromboxane A2 synthase inhibitor, CGS14854. (rti.org)
  • Cultured B16a cells maintained at low density (LD) and harvested at low passage (LP) formed significantly more lung colonies than B16a cells harvested at high densities (HD) or high passage (HP). (rti.org)
  • Over-confluent tumor cells produced even lower number of lung colonies. (rti.org)
  • Lung colony formation by elutriated B16a cells (i.e., cells freshly isolated from tumor tissue) was consistently inhibited by CGS14854, whereas inhibition of lung colony formation by cultured B16a cells was dependent upon culture conditions. (rti.org)
  • The differences in lung colony formation between LD, HD and elutriated B16a cells were not due to differential cell-cycle distribution. (rti.org)
  • collagen
  • In this study, we successfully cultured ACC cells in collagen gel and examined the process of pseudocyst formation in tumor cell colonies. (nii.ac.jp)
  • At the same time, we examined xenografts of ACC2 in SCID mice and compared the nature of pseudocystic spaces formed in the transplants with that in the collagen gel cultures. (nii.ac.jp)
  • In the collagen gel culture, the cells formed spherical colonies and the tumor cell nests contained vacuolar structures that were immunopositive for heparan sulfate proteoglycan, type III collagen and type IV collagen, and fibronectin. (nii.ac.jp)
  • Publications] Munakata, R.: 'Pseudocyst tormation by adenoid cystic carcinoma cells collagen gel culture and in SCID mice' J Oral Pathol Med. (nii.ac.jp)
  • determinants
  • The oligosaccharide groups on gangliosides extend well beyond the surfaces of the cell membranes, and act as distinguishing surface markers that can serve as specific determinants in cellular recognition and cell-to-cell communication. (wikipedia.org)
  • Furthermore
  • Furthermore, cells can sort based upon differences in adhesion between the cells, so even two populations of cells with different levels of the same adhesion molecule can sort out. (wikipedia.org)
  • therapeutic
  • The ability to effectively culture CTCs and examine them for genetic changes can be used to find targetable mutations and discover which therapeutic drugs are most effective. (oncologynurseadvisor.com)
  • processes
  • IMC is sensitive enough to detect and follow either slow processes (reactions proceeding at a few % per year) in a few grams of material, or processes which generate minuscule amounts of heat (e.g. metabolism of a few thousand living cells). (wikipedia.org)
  • vascular
  • It provides a feasible mechanism of early tumour vascular supply which can co-exist and incorporate with later angiogenic mechanisms. (biomedsearch.com)
  • patients
  • Patients whose tumors express resistance to chemotherapeutic drugs tend to do so in one of two ways, either intrinsically or after showing an initial response. (aacrjournals.org)
  • selectively
  • After losing energy as they penetrate tissue, the neutrons are absorbed by the capture agent, which subsequently emits high-energy charged particles that can selectively kill tumor cells that have taken up sufficient quantities of 10B (Fig.1). (wikipedia.org)
  • refractory
  • This malignancy is a paradigm for refractory and incurable solid tumors at all ages because more than half of children with RMS at diagnosis have either regional lymph node or distant metastases ( 2 ). (aacrjournals.org)
  • As of 2016[update] panobinostat is being studied in a phase II trial for relapsed and refractory diffuse large B-cell lymphoma (DLBCL). (wikipedia.org)
  • laboratory
  • After culturing and isolating the organism in laboratory mice, the pathogen they named Rickettsia akari was identified as the ultimate cause of the disease now called rickettsialpox. (wikipedia.org)
  • inhibitors
  • For Western blotting, tumor tissues from mice were collected in radioimmunoprecipitation (RIPA) buffer supplemented with a cocktail of protease inhibitors and Serine/Threonine and Tyrosine phosphatase inhibitors (Thermo Fisher Scientific). (aacrjournals.org)
  • tissues
  • For example, during gastrulation, clumps of stem cells switch off their cell-to-cell adhesion, become migratory, and take up new positions within an embryo where they again activate specific cell adhesion proteins and form new tissues and organs. (wikipedia.org)
  • Morphogenesis also involves changes in the cellular structure or how cells interact in tissues. (wikipedia.org)
  • The success of BNCT is dependent upon the selective delivery of sufficient amounts of 10B to the tumor with only small amounts localized in the surrounding normal tissues. (wikipedia.org)
  • cancer
  • Researchers based at the Massachusetts General Hospital (MGH) Center for Engineering in Medicine and the MGH Cancer Center have created a microchip device, called the CTC-iChip, which can capture circulating tumor cells in order to track ongoing genetic mutation within a tumor. (oncologynurseadvisor.com)
  • Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
  • Cancer cells do not exist in isolation. (hindawi.com)
  • PS-341 has been found to have good broad-spectrum cytotoxic activity in the 60-monolayer cell line National Cancer Institute screen. (aacrjournals.org)
  • partially reversing the morphological changes characteristic of breast cancer cells. (wikipedia.org)
  • February 23, 1914 - August 26, 1998), was an American physician and virologist whose research into viruses, their causes and treatment that led to his breakthrough insights into the connections between viruses and cancer, leading to new treatments, as well as his hypothesized oncogene, which was discovered to be a trigger for normal cells turning cancerous. (wikipedia.org)
  • Each component in itself is non-tumoricidal, but when combined together they are highly lethal to cancer cells. (wikipedia.org)
  • biological activity
  • INH1, a thiazolyl benzamide compound, is a cell-permeable Hec1/Nek2 mitotic pathway inhibitor I. INH1 controls the biological activity of Hec1/Nek2 mitotic pathway. (wikipedia.org)
  • primary
  • Saos-2 ("Sarcoma osteogenic") is a cell line derived from the primary osteosarcoma of an 11-year-old Caucasian girl in 1973 by Fogh et al. (wikipedia.org)
  • The village was primary designed for tourism to promote African culture and was generally ignored by the Sowetan locals, partly due to the unfamiliarity of the mythology that was being represented. (wikipedia.org)