• proliferation
  • The retention of abundant basement membrane molecules in the stromal space that includes pseudocystic cavities suggests that basement membrane matrix is essential for proliferation of adenoid cystic carcinoma cells. (nii.ac.jp)
  • Because rapid tumor proliferation is associated with poor patient prognosis, here we characterized the transcriptional signature of rapidly proliferating colorectal cancer cells in an attempt to identify novel candidate therapeutic targets. (aacrjournals.org)
  • The conversion of cell membrane arachidonic acid (20:4n-6) to omega-6 prostaglandin and omega-6 leukotriene eicosanoids during the inflammatory cascade provides many targets for pharmaceutical drugs to impede the inflammatory process in atherosclerosis, asthma, arthritis, vascular disease, thrombosis, immune-inflammatory processes, and tumor proliferation. (wikipedia.org)
  • A ratio of 2.5:1 reduced rectal cell proliferation in patients with colorectal cancer, whereas a ratio of 4:1 had no effect. (wikipedia.org)
  • Experiments show that INH1 potently inhibits the proliferation of multiple human breast cancer cell lines, cervical HeLa cells, and colon cancer cells in vitro. (wikipedia.org)
  • These formazan dyes are commonly used in cell proliferation and toxicity assays like the EpiDerm and EpiSkin tests since they only stain living, metabolically active cells. (wikipedia.org)
  • The role for the Wnt/β-catenin signaling in cancer has been well defined: β-catenin drives transcription of genes that contribute to the tumor phenotype by regulating processes such as proliferation, survival and invasion. (wikipedia.org)
  • The growth-inhibitory effects of SFRP1 were due primarily to decreased cell proliferation rather than an increase in apoptosis. (wikipedia.org)
  • brain tumors
  • Conversely, the presence of AGT protein in brain tumors predicts poor response to temozolomide and these patients receive little benefit from chemotherapy with temozolomide. (wikipedia.org)
  • Neutron capture therapy (NCT) is a noninvasive therapeutic modality for treating locally invasive malignant tumors such as primary brain tumors and recurrent head and neck cancer. (wikipedia.org)
  • BNCT has been evaluated clinically as an alternative to conventional radiation therapy for the treatment of malignant brain tumors (gliomas), and recurrent, locally advanced head and neck cancer. (wikipedia.org)
  • W. H. Sweet, from Massachusetts General Hospital, first suggested the technique for treating malignant brain tumors and a trial of BNCT against the most malignant of all brain tumors, glioblastoma multiforme, using borax as the delivery agent in 1951. (wikipedia.org)
  • malignant
  • Hypoxia was further demonstrated to cause a more malignant anchorage-independent growth phenotype in the resistant cells, which can be abolished by knocking down ABCG2. (hindawi.com)
  • Panobinostat inhibits multiple histone deacetylase enzymes, a mechanism leading to apoptosis of malignant cells via multiple pathways. (wikipedia.org)
  • As of August 2012[update], it is being tested against Hodgkin's Lymphoma, cutaneous T cell lymphoma (CTCL) and other types of malignant disease in Phase III clinical trials, against myelodysplastic syndromes, breast cancer and prostate cancer in Phase II trials, and against chronic myelomonocytic leukemia (CMML) in a Phase I trial. (wikipedia.org)
  • Moreover, a recent study with various malignant tumor cells showed that celecoxib could inhibit the growth of these cells, even though some of these cancer cells didn't even contain COX-2. (wikipedia.org)
  • apoptosis
  • Western blot analysis was performed to study Boswellia sacra essential oil-regulated proteins involved in apoptosis, signaling pathways, and cell cycle regulation. (biomedcentral.com)
  • Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
  • Once delivered, granzymes are able to induce apoptosis and cause target cell death. (wikipedia.org)
  • INH1 induces a transient mitotic arrest, exhibiting metaphase chromosome misalignment, spindle abnormality, and consequently cancer cell apoptosis. (wikipedia.org)
  • vitro
  • Cells grown in vitro from neoplastic tissue. (jove.com)
  • Overexpression of ABCG2 in cancer cell lines in vitro has been shown to confer MDR to a variety of anticancer drugs including mitoxantrone, irinotecan, methotrexate, flavopiridol, and anthracyclines [ 1 ]. (hindawi.com)
  • Panobinostat has also been found to significantly increase in vitro the survival of motor neuron (SMN) protein levels in cells of patients suffering from spinal muscular atrophy. (wikipedia.org)
  • A 2015 study suggested Panobinostat was effective in preventing diffuse intrinsic pontine glioma cell growth in vitro and in vivo, identifying it as a potential drug candidate. (wikipedia.org)
  • The publication of two seminal research papers in 1998 outlining the isolation of these cells provided an in vitro method by which researchers may characterise PaSCs in both health and pathology. (wikipedia.org)
  • epigenetic
  • Importantly, glucose deprivation and hypoxia were also found to enhance the resistance level of ABCG2-overexpressing resistant cells with pre-existing genetic and epigenetic MDR mechanisms. (hindawi.com)
  • In some tumors, epigenetic silencing of the MGMT gene prevents the synthesis of this enzyme, and as a consequence such tumors are more sensitive to killing by temozolomide. (wikipedia.org)
  • molecules
  • The pseudocysts are surrounded by tumor cells and are filled with extracellular matrix components such as basement membrane molecules. (nii.ac.jp)
  • We have demonstrated that these basement membrane molecules were produced by ACC2 and ACC3 cells, established independently from human salivary adenoid cystic carcinomas. (nii.ac.jp)
  • We have also shown that adenoid cystic carcinoma dells attach, spread, and grow rapidly on matrices conta … More ining basement membrane molecules, using ACC3 cells of human adenoid cystic carcinoma origin. (nii.ac.jp)
  • The results indicate that our collagen gel culture system provides physiological conditions for ACC2 cells to secrete particular extracelluar matrix molecules and form pseudocystic spaces. (nii.ac.jp)
  • Publications] Cheng, J.: 'Biosynthesis of basement membrane molecules by salivary adenoid cystic carcinoma cells : an immunofluorescence and confocal microscopic study. (nii.ac.jp)
  • Tumor microenvironment represents an attractive new drug target because it allows complex interaction between a tumor and its surrounding normal cells, molecules, and blood vessels, which all participate in tumor progression. (hindawi.com)
  • They can be thought of as inhabiting within a complex milieu of normal cells, blood vessels, endogenous small molecules, and secreted factors, which collectively comprise the tumor microenvironment. (hindawi.com)
  • determined that Saos-2 cells "possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules. (wikipedia.org)
  • An important class of molecules involved in morphogenesis are transcription factor proteins that determine the fate of cells by interacting with DNA. (wikipedia.org)
  • At the end of this cascade are classes of molecules that control cellular behaviors such as cell migration, or, more generally, their properties, such as cell adhesion or cell contractility. (wikipedia.org)
  • One of the ways this can occur is when cells share the same cell-to-cell adhesion molecules. (wikipedia.org)
  • For instance, homotypic cell adhesion can maintain boundaries between groups of cells that have different adhesion molecules. (wikipedia.org)
  • Gangliosides are present and concentrated on cell surfaces, with the two hydrocarbon chains of the ceramide moiety embedded in the plasma membrane and the oligosaccharides located on the extracellular surface, where they present points of recognition for extracellular molecules or surfaces of neighboring cells. (wikipedia.org)
  • inhibit
  • The endothelial growth factor antibodies used did not inhibit or stimulate cell growth when compared to control but did discourage vascular mimicry. (biomedsearch.com)
  • However, when the ability of all these compounds to kill tumor cells in cell culture was investigated, it turned out that the antitumor potency did not at all depend on whether or not the respective compound could inhibit COX-2, showing that inhibition of COX-2 was not required for the anticancer effects. (wikipedia.org)
  • One of these compounds, 2,5-dimethyl-celecoxib, which entirely lacks the ability to inhibit COX-2, actually turned out to display stronger anticancer activity than celecoxib itself and this anticancer effect could also be verified in highly drug-resistant tumor cells and in various animal tumor models. (wikipedia.org)
  • mediates
  • Furthermore, these results highlight the importance of pathologically relevant tissue culture models to study the complex physical and chemical processes by which the cancer microenvironment mediates new vessel formation. (sigmaaldrich.com)
  • The activation of P2 receptors induces intracellular calcium signalling which mediates the fibrogenic function of activated stellate cells. (wikipedia.org)
  • collagen
  • RESULTS: There were observable differences in growth of the cells on laboratory plastic and collagen matrix. (biomedsearch.com)
  • In this study, we successfully cultured ACC cells in collagen gel and examined the process of pseudocyst formation in tumor cell colonies. (nii.ac.jp)
  • At the same time, we examined xenografts of ACC2 in SCID mice and compared the nature of pseudocystic spaces formed in the transplants with that in the collagen gel cultures. (nii.ac.jp)
  • In the collagen gel culture, the cells formed spherical colonies and the tumor cell nests contained vacuolar structures that were immunopositive for heparan sulfate proteoglycan, type III collagen and type IV collagen, and fibronectin. (nii.ac.jp)
  • Publications] Munakata, R.: 'Pseudocyst tormation by adenoid cystic carcinoma cells collagen gel culture and in SCID mice' J Oral Pathol Med. (nii.ac.jp)
  • pathways
  • Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
  • lines
  • Vascular mimicry in cultured head and neck tumour cell lines. (biomedsearch.com)
  • We suggest this is common phenomenon in head and neck squamous cell carcinoma (HNSCC) cell lines and other aggressive tumour cell lines. (biomedsearch.com)
  • We present experimental evidence of vasculogenic mimicry in HNSCC cell lines and compare them with other tumours and a positive control vascular cell line. (biomedsearch.com)
  • Other tumour cell lines also displayed this property. (biomedsearch.com)
  • The focus of this study was to examine the CTC-iChip and determine if the device could properly capture CTCs to aid the formation of cell lines to represent the genetic status of all existing tumor sites. (oncologynurseadvisor.com)
  • Cell lines with longevity were successfully established from 6 patients. (oncologynurseadvisor.com)
  • Additional samples from 3 of these patients were used to set up new cell lines to track the effectiveness of therapy via tumor change. (oncologynurseadvisor.com)
  • Circulating tumor cells captured with a microchip-based device developed at the Massachusetts General Hospital (MGH) Center for Engineering in Medicine and the MGH Cancer Center can be cultured to establish cell lines for genetic analysis and drug testing. (oncologynurseadvisor.com)
  • Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
  • All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
  • protein
  • However, some tumor cells are able to repair this type of DNA damage, and therefore diminish the therapeutic efficacy of temozolomide, by expressing a protein O6-alkylguanine DNA alkyltransferase (AGT) encoded in humans by the O-6-methylguanine-DNA methyltransferase (MGMT) gene. (wikipedia.org)
  • determinants
  • The oligosaccharide groups on gangliosides extend well beyond the surfaces of the cell membranes, and act as distinguishing surface markers that can serve as specific determinants in cellular recognition and cell-to-cell communication. (wikipedia.org)
  • human
  • The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
  • Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)
  • Primary Human Cytomegalovirus Infection Induces the Expansion of Virus-Specific Activated and Atypical Memory B Cells. (ac.be)
  • It has been shown that the deregulation of this critical developmental pathway occurs in several human tumor entities. (wikipedia.org)
  • therapeutic
  • The ability to effectively culture CTCs and examine them for genetic changes can be used to find targetable mutations and discover which therapeutic drugs are most effective. (oncologynurseadvisor.com)
  • processes
  • IMC is sensitive enough to detect and follow either slow processes (reactions proceeding at a few % per year) in a few grams of material, or processes which generate minuscule amounts of heat (e.g. metabolism of a few thousand living cells). (wikipedia.org)
  • vascular
  • It provides a feasible mechanism of early tumour vascular supply which can co-exist and incorporate with later angiogenic mechanisms. (biomedsearch.com)
  • ultimately
  • It may also contribute to the maintenance of stem or progenitor cells as a survival factor, ultimately driving tumor growth [ 4 ]. (hindawi.com)
  • Ultimately the ganglion cells in the nervous system swell enormously, disturbing the normal functions of neurons. (wikipedia.org)
  • selectively
  • After losing energy as they penetrate tissue, the neutrons are absorbed by the capture agent, which subsequently emits high-energy charged particles that can selectively kill tumor cells that have taken up sufficient quantities of 10B (Fig.1). (wikipedia.org)
  • colonies
  • Prolonged culture resulted in a 10-fold decrease in the incidence of B16a lung colonies, whereas passage in vivo for 150 days did not affect lung colony formation by tumor cells isolated from enzymatic dispersates by centrifugal elutriation. (rti.org)
  • Cultured B16a cells maintained at low density (LD) and harvested at low passage (LP) formed significantly more lung colonies than B16a cells harvested at high densities (HD) or high passage (HP). (rti.org)
  • Over-confluent tumor cells produced even lower number of lung colonies. (rti.org)
  • growth
  • We recently developed a simple but unique microfluidics-based culture approach that requires minimal preprocessing (~30 min) and does not require prior enrichment of CTC s or depend on the use of growth factor supplements. (nus.edu.sg)
  • It is also believed to be a survival factor for cancer stem cells to drive tumor growth. (hindawi.com)
  • This study investigated the upregulation of ABCG2 by these adverse growth conditions within the tumor microenvironment. (hindawi.com)
  • The biological effects of the omega-6 fatty acids are largely produced during and after physical activity for the purpose of promoting growth and during the inflammatory cascade to halt cell damage and promote cell repair by their conversion to omega-6 eicosanoids that bind to diverse receptors found in every tissue of the body. (wikipedia.org)
  • but studies in laboratory mice have shown that nelfinavir is able to suppress the growth of tumors in these animals, which represents a promising lead towards testing this drug in humans as well. (wikipedia.org)
  • In biological specimens containing a growth medium, appearance over time of a detectable and rising heat flow signal is a simple general indicator of the presence of some type of replicating cells. (wikipedia.org)
  • Growth in culture and anchorage-independent growth were inhibited in SFRP1-expressing UMRC3 cells. (wikipedia.org)
  • laboratory
  • After culturing and isolating the organism in laboratory mice, the pathogen they named Rickettsia akari was identified as the ultimate cause of the disease now called rickettsialpox. (wikipedia.org)
  • Carcinoma
  • Adenoid cystic carcinoma is histologically characterized by a cribriform appearance that results from formation of multi-pseudocystic spaces in each tumor cell nest. (nii.ac.jp)
  • We have proposed that their frequent invasion of peripheral nerves, blood vessels, and skeletal muscles can be interpreted to mean that adenoid cystic carcinoma cells have an affinity for basement membranes. (nii.ac.jp)
  • Thus, we planned to establish a three-dimension-culture system of adenoid cystic carcinoma cells in which the tumor nest architecture could be analyzed in a longer period than a monolayr culture system. (nii.ac.jp)
  • Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto, and Naoki Ishige, "Hepatocyte selection medium-enriched hepatocellular carcinoma cells are positive for α-fetoprotein and CD44. (hindawi.com)
  • This gene is located within chromosome 9 (9q32-33), a chromosomal region that frequently shows loss of heterozygosity in transitional cell carcinoma of the bladder. (wikipedia.org)
  • vivo
  • A nticancer drug efficacy has been tested on circulating tumor cells ( CTC s) derived from patient blood samples after ex vivo expansion into CTC clusters. (nus.edu.sg)
  • primary
  • Circulating tumor cells (CTCs) are living solid-tumor cells that separate from either primary or secondary tumors and go on to move through the bloodstream. (oncologynurseadvisor.com)
  • Saos-2 ("Sarcoma osteogenic") is a cell line derived from the primary osteosarcoma of an 11-year-old Caucasian girl in 1973 by Fogh et al. (wikipedia.org)
  • The village was primary designed for tourism to promote African culture and was generally ignored by the Sowetan locals, partly due to the unfamiliarity of the mythology that was being represented. (wikipedia.org)
  • platelets
  • Mechanistic studies indicated that LD/LP tumor cells induced aggregation of homologous platelets, whereas HD/HP B16a cells failed to induce significant platelet aggregation. (rti.org)
  • membranes
  • The MACPF domain is structurally similar to pore-forming cholesterol-dependent cytolysins from gram-positive bacteria, suggesting that MACPF proteins create pores and disrupt cell membranes similar to cytolysin. (wikipedia.org)
  • proteins
  • Basic proteins and polyamino acids are taken up by mammalian cells at rates up to 3000 times greater than serum albumin. (sciencemag.org)
  • For example, during gastrulation, clumps of stem cells switch off their cell-to-cell adhesion, become migratory, and take up new positions within an embryo where they again activate specific cell adhesion proteins and form new tissues and organs. (wikipedia.org)
  • Stellate cells express smoothened (Smo) and patched-1 (Ptch1) proteins, which are significant features of the hedgehog receptor system. (wikipedia.org)
  • mammalian
  • Mammalian cells lack the enzyme omega-3 desaturase and therefore cannot convert omega-6 fatty acids to omega-3 fatty acids. (wikipedia.org)