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  • digestive
  • Trypsin (EC 3.4.21.4) is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins. (wikipedia.org)
  • Cholecystokinin, a hormone secreted by the APUD cells located in the proximal mucosa of the small intestine is administered intravenously, this stimulates the pancreatic secretion of the digestive enzymes amylase, trypsin, and lipase. (wikipedia.org)
  • proteins
  • Long term incubation with high trypsin concentration damage cells by striping cell surface proteins and kill the cells. (sigmaaldrich.com)
  • In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). (purdue.edu)
  • The process is commonly referred to as trypsin proteolysis or trypsinisation, and proteins that have been digested/treated with trypsin are said to have been trypsinized. (wikipedia.org)
  • In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. (wikipedia.org)
  • This means that trypsin predominantly cleaves proteins at the carboxyl side (or "C-terminal side") of the amino acids lysine and arginine except when either is bound to a C-terminal proline, although large-scale mass spectrometry data suggest cleavage occurs even with proline. (wikipedia.org)
  • These human genes encode proteins with trypsin enzymatic activity: Other isoforms of trypsin may also be found in other organisms. (wikipedia.org)
  • Enteropeptidase exhibits trypsin-like activity, cleaving proteins following a lysine at a specific cleavage site (Asp-Asp-Asp-Asp-Lys). (wikipedia.org)
  • EDTA
  • To weaken the interactions, EDTA is frequently included with Trypsin which chelates the divalent cations (Ca 2+ , Mg 2+ ). (sigmaaldrich.com)
  • 1. Bring the DPBS, the Trypsin-EDTA for Primary Cells, and the Trypsin Neutralizing Solution to room temperature before use. (atcc.org)
  • If the cell culture medium contains serum, each flask should be rinsed with DPBS twice prior to adding the Trypsin-EDTA for Primary Cells. (atcc.org)
  • 3. Using 1 to 2 mL for every 25 cm 2 , add the appropriate volume of trypsin-EDTA solution to each flask (e.g., each T-25 flask would be dissociated with 1 to 2 mL trypsin-EDTA). (atcc.org)
  • Gently pipette or swirl the culture to ensure all of the trypsin-EDTA solution has been neutralized. (atcc.org)
  • 9. Transfer the cell / DPBS suspension to the centrifuge tube containing the trypsin-EDTA-dissociated cells. (atcc.org)
  • Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots. (atcc.org)
  • Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. (atcc.org)
  • Most commonly, researchers use trypsin, sometimes in combination with EDTA, to separate the adherent cells from their culture substrate and from each other. (sigmaaldrich.com)
  • cleaves
  • This version of the kit uses trypsin, which cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. (thermofisher.com)
  • autodigestion
  • This modern formulation of trypsin is stable at room temperature, but performs identically to traditional trypsin solutions that must be stored frozen at -20°C to prevent autodigestion. (sigmaaldrich.com)
  • enteropeptidase
  • As the pro-region of trypsinogen contains this sequence, enteropeptidase catalyses its activation in vivo: trypsinogentrypsin + pro-region (Val-Asp-Asp-Asp-Asp-Lys) In humans, enteropeptidase is encoded by the PRSS7 gene (also known as ENTK) on chromosome 21q21. (wikipedia.org)
  • amino
  • Trypsin is considered an endopeptidase, i.e., the cleavage occurs within the polypeptide chain rather than at the terminal amino acids located at the ends of polypeptides. (wikipedia.org)
  • flask
  • Add appropriate quantity (0.5 ml/10cm 2 ) of pre-warmed trypsin solution to the side wall of the flask. (sigmaaldrich.com)
  • 6. When the majority of cells appear to have detached, quickly add an equal volume of the Trypsin Neutralizing Solution to each flask. (atcc.org)