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  • Protocol
  • Azidine blue 3B Benzamine blue 3B Benzo Blue bB Chlorazol blue 3B Diamine blue 3B Dianil blue H3G Direct blue 14 Niagara blue 3B Chapter "Detection of Caspase Activation Combined with Other Probes of Apoptosis", Eurekah Bioscience Collection, NCBI bookshelf Protocol for use of the dye (PDF) from Northwestern University Wainwright, M. (December 2010). (wikipedia.org)
  • In this protocol plant tissue is stained with trypan blue dye allowing the researcher to visualize cell death. (bio-protocol.org)
  • injection
  • In early 20th century, the existence of a barrier protective toward the brain ( blood brain barrier ) was inferred, based on the observation that injection of trypan blue in animals led to whole‐body staining except for the brain and spinal cord. (wikipedia.org)
  • time
  • Over time trypan blue naturally forms dye aggregates and crystals, it is therefore recommended that TB is filtered using a 0.2 micron filter prior to use. (nexcelom.com)
  • blood
  • Vitellogenic oocytes can also sequester the acidic colloid trypan blue: when injected into the blood, the dye, like the blood proteins, is deposited in yolk spheres in the cortex of the oocyte. (biologists.org)
  • cells
  • Results of reagents Rabbit Polyclonal to BST2 on cell viability had been sized by plating cells at 12% confluence and developing right away on regular 12 well tissues lifestyle plate designs (Cyto One Closed circuit7682-7512), dealing with for 24 hours with MASL (Sentrimed) or NZ-1 (ready as defined [46, 53, 104, , and keeping track of cells after yellowing with Trypan blue. (world-of-links.com)
  • Hence, dead cells appear as a distinctive blue colour under a microscope. (wikipedia.org)