• injection
  • In early 20th century, the existence of a barrier protective toward the brain (blood brain barrier) was inferred upon based on the observation that injection of trypan blue to animals led to whole‐body staining except for the brain and spinal cord. (wikipedia.org)
  • Detection
  • Azidine blue 3B Benzamine blue 3B Benzo Blue bB Chlorazol blue 3B Diamine blue 3B Dianil blue H3G Direct blue 14 Niagara blue 3B Chapter "Detection of Caspase Activation Combined with Other Probes of Apoptosis", Eurekah Bioscience Collection, NCBI bookshelf Protocol for use of the dye (PDF) from Northwestern University Wainwright, M. (December 2010). (wikipedia.org)
  • method
  • IC50 value determined by Trypan Blue method is 35,34 µg lindane/mL of cultivating medium, and determined by Kenacid Blue method is 74,08 µg lindane/mL of cultivating medium. (unizg.hr)
  • long
  • Most microscopic procedures involving trypan blue staining require a long subsequent clearing step using chloral hydrate (CHL), a small organic compound currently used such as a carcinogen, an anesthetic and an analgesic in laboratory animals (Keogh et al . (bio-protocol.org)
  • blood
  • Vitellogenic oocytes can also sequester the acidic colloid trypan blue: when injected into the blood, the dye, like the blood proteins, is deposited in yolk spheres in the cortex of the oocyte. (biologists.org)