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  • gene
  • Different applications of gene electrotransfer require high viability of cells and high transfection efficiency of gene electrotransfer. (springer.com)
  • It was already demonstrated that the addition of fetal bovine serum (FBS) immediately after gene electrotransfer leads to improved cell survival and transfection efficiency. (springer.com)
  • On the other hand, transfection efficiency of gene electrotransfer was not affected by long incubation of cell in FBS, regardless of the cell line used. (springer.com)
  • The results of our study emphasize the importance of optimization of gene electrotransfer protocol for particular cells and specific purposes of gene electrotransfer, taking into account the importance of transfection efficiency and cell survival. (springer.com)
  • cell
  • Therefore, the aim of the study was to determine whether prolonged incubation of cells in FBS, for more than standard 5 min, can lead to increased transfection efficiency and improved cell survival. (springer.com)
  • Transfection efficiency was determined by flow cytometry and fluorescence microscopy and cell survival by cell viability assay. (springer.com)
  • uptake
  • However, with the invention of different types of microarray printing systems, hundreds of transfection mixes (containing different DNA of interest) may be printed on the same slide for cell uptake of plasmids. (wikipedia.org)
  • Methods
  • While a virus has the potential to transmit these foreign items through a cell's membrane and into the cell, transfection uses methods other than a virus for transmission. (wisegeek.com)
  • Altogen Biosystems began as a company focusing on non-viral methods of transfection. (wikipedia.org)
  • RNAi
  • Transfection of RNA can be used either to induce protein expression, or to repress it using antisense or RNA interference (RNAi) procedures. (qiagen.com)
  • proteins
  • Nucleic acids commonly used in transfection include DNA , RNA , and proteins, among other materials. (wisegeek.com)
  • Inhibiting only three proteins, interferon-β, STAT2, and EIF2AK2 is sufficient to rescue human fibroblasts from the cell death caused by frequent transfection with long, protein-encoding RNA. (wikipedia.org)
  • Polymers
  • Chemical-based transfection can be divided into several kinds: cyclodextrin, polymers, liposomes, or nanoparticles (with or without chemical or viral functionalization. (wikipedia.org)
  • biological
  • To be in line with the current definition of transfection in the biological community, non-nucleic acids (such as fluorophores) cannot, by definition, be optically transfected (only optically injected). (wikipedia.org)
  • cell types
  • Since this time, the optical transfection of a host of mammalian cell types has been demonstrated using a variety of laser sources, including the 405 nm continuous wave (cw), 488 nm cw, or pulsed sources such as the 800 nm femtosecond pulsed Ti:Sapphire or 1064 nm nanosecod pulsed Nd:YAG. (wikipedia.org)
  • second
  • The first term of the technique is invariably a derivation of word laser, optical, or photo, and the second term is usually in reference to injection, transfection, poration, perforation or puncture. (wikipedia.org)