Transcriptional activation. Medical search. Frequent questions

Taken together, the data from the present work shed a light on the transcriptional regulation of DegS-DegU two-component system in B. subtilis and implicated intracellular glutamate as a positive stimulus involved as a transducer of the information from the environment to the intracellular apparatus. (uni-marburg.de)
Therefore, investigating LINE-1 transcriptional regulation is important for our understanding of LINE-1's role in cancer. (bmj.com)
Conclusions Findings from our study increase our understanding of the transcriptional regulation of different mouse LINE-1 subfamilies, highlight the differing roles of YY1 in regulating human and mouse L1 transcription, and have important implications in the role of LINE-1s during cancer development. (bmj.com)
Lis, J. Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation. (nature.com)
The role of basal and myogenic factors in the transcriptional activation of utrophin promoter A: implications for therapeutic up-regulation in Duchenne muscular dystrophy. (ox.ac.uk)
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (cancerindex.org)
The purpose of this study was to clarify the roles of paraspeckles in PXR-mediated transcriptional regulation. (aspetjournals.org)
The major events in the regulation of the host response on a transcriptional level occur within the first 3 days after infection. (biomedcentral.com)
Activation of Btk results in a cascade of signaling events resulting in calcium mobilization and fluxes, cytoskeletal rearrangements, and transcriptional regulation involving nuclear factor-kappaB (NF-kappaB) and nuclear factor of activated T cells (NFAT). (medscape.com)
We provide a new mechanism for the regulation of heart development by PITX2 isoforms through the regulation of ANF and PLOD1 gene expression and Nkx2.5 transcriptional activity. (lu.se)

The Tet-Off system makes use of the tetracycline transactivator (tTA) protein, which is created by fusing one protein, TetR (tetracycline repressor), found in Escherichia coli bacteria, with the activation domain of another protein, VP16, found in the herpes simplex virus. (wikipedia.org)
Pax5 (BSAP) is essential for B cell development and acts both as a transcriptional activator and a repressor. (elsevierpure.com)
These data suggest that Daxx can affect Pax5's roles as an activator or repressor in B cells and describe a role for Daxx as a transcriptional coactivator. (elsevierpure.com)
It uses transcription activator-like effector (TALE) technology combined with VP64 activator or Kruppel-associated box (KRAB) repressor, both of which are potent transcriptional regulators that modify the epigenetic state of endogenous DNA loci. (springer.com)
Cong L, Zhou R, Kuo YC et al (2012) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. (springer.com)

In plants and Schizosaccharomyces pombe , small interfering RNA (siRNA)-directed chromatin modifications (DNA methylation for plants and H3K9 methylation [H3K9me] for S. pombe ) lead to transcriptional repression. (biomedcentral.com)
This protocol describes methods to design, assemble, and validate tools for targeted activation or repression of single-copy and multi-copy genes, including repetitive and transposable elements. (springer.com)

combinatorial complexes led to chromatin remodeling and transcriptional induction of prognostic genes. (tcd.ie)
We also tested DNA-binding, transiently expressed activation-associated proteins (AAPs) that are known to support an open, transcriptionally active chromatin state. (aip.org)
AML has a characteristic dependency on chromatin and transcriptional regulators, which has been discussed in many reviews. (frontiersin.org)
Jachowicz JW, Bing X, Pontabry J et al (2017) Activation of LINE-1 after fertilisation regulates global chromatin accessibility. (springer.com)

These results indicate that YY1 is a transcriptional activator of mouse LINE-1 Tf_I subfamily. (bmj.com)
Reporter gene assays show that ZNF322 is a transcriptional activator. (anthropogeny.org)
Effects of EGCG on nuclear factor kappa B (NFκB) and activator protein-1 (AP-1) transcriptional activity were assessed by reporter gene assay. (molvis.org)

Although pausing has been connected to extremely rapid and synchronous activation of genes, pausing is also highly associated with constitutively expressed genes that encode signalling and transcription factors. (nature.com)
Distinct signals that act through diverse targeted transcription factors can regulate different steps in the transcription pathway and provide a highly modulated transcriptional response at individual genes. (nature.com)
By examining the mRNA, small RNA, RNA polymerase II, and H3K9 trimethylation profiles at the whole-genome level, we revealed an epigenetic role of HRDE-1 in repressing heat stress-induced transcriptional activation of over 280 genes. (biomedcentral.com)
Strikingly, for some of these genes, the heat stress-induced transcriptional activation in the hrde - 1 mutant intensifies in the late generations under the heat stress and is heritable for at least two generations after the mutant animals are shifted back to lower temperature. (biomedcentral.com)
Maeder ML, Angstman JF, Richardson ME et al (2013) Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. (springer.com)

Here we found that some physiologically important transcriptional regulators of Pseudomonas aeruginosa including QscR, a quorum sensing (QS) receptor, SoxR, a superoxide sensorregulator, and AntR, a regulator of anthranilate-related secondary metabolism, are activated by various growthrestricted conditions. (molcells.org)
However, LasR and PqsR, other QS receptors of P. aeruginosa , were not activated, suggesting that only a specific set of transcriptional regulators is activated by growth restriction. (molcells.org)
A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. (umu.se)

In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)

The silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) is an established histone deacetylase 3 (HDAC3)-dependent transcriptional corepressor. (nih.gov)
A component of promyelocytic leukemia protein nuclear bodies, Daxx has been implicated in apoptosis and characterized as a transcriptional corepressor. (elsevierpure.com)

Autocrine transforming growth factor-{beta}1 activation mediated by integrin {alpha}V{beta}3 regulates transcriptional expression of laminin-332 in Madin-Darby canine kidney epithelial cells. (uchicago.edu)
ZNF322, a novel human C2H2 Kruppel-like zinc-finger protein, regulates transcriptional activation in MAPK signaling pathways. (anthropogeny.org)

Transient transfection of the OST promoter in differentiating MC3T3 results in a significant increase in transcriptional activation from day 0 to day 5 of differentiation, similar in timing and intensity to the observed upregulation of the endogenous gene. (umn.edu)
Here we describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. (cdc.gov)
Upon transient transfection assay of Daxx in B cells expressing endogenous Daxx and Pax5, we observed not only transcriptional corepression but also, unexpectedly, coactivation in M12.4.1 and A20 mouse B cell lines. (elsevierpure.com)
Our study demonstrated that a large number of the endogenous targets of the germline nuclear RNAi pathway in C. elegans are sensitive to heat-induced transcriptional activation. (biomedcentral.com)

Genotoxic damage causes robust alterations to pathways associated with B cell activation and increased proliferation, suggesting that genotoxic damage initiates not only the normal B cell maturation processes but also mimics activated B cell response to antigenic agents. (biomedcentral.com)

Reintroduction of APC removed beta-catenin from hTcf-4 and abrogated the transcriptional transactivation. (bioseek.eu)
We found that the shedding of heparin-binding epidermal growth factor (HB-EGF) resulting from metalloproteinase activation and subsequent transactivation of the epidermal growth factor receptor occurred when cardiomyocytes were stimulated by GPCR agonists, leading to cardiac hypertrophy. (nih.gov)
The NTD of the hGRα contains a major transactivation domain, termed activation function (AF)-1, which is located between amino acids 77 and 262 of the hGRα and is ligand-independent. (medscape.com)

In parallel to exploring the role of the DegS-DegU system at the transcriptional and at the protein level, some additional experiments were performed in order to identify possible downstream regulated targets of the system. (uni-marburg.de)
Non (protein)-coding RNAs are the most abundant transcriptional products of the coding genome, and comprise several different classes of molecules with unique lengths, conformations and targets. (frontiersin.org)
Its activation is tightly controlled by numerous other signaling proteins including protein kinase C (PKC), Sab/SH3BP, and caveolin-1. (medscape.com)

Investigations of the main degS-degU promoter in the presence of different charged and non-charged compound, which raised the surrounding osmolarity to an equal extent, demonstrated that the activation of the system is rather an osmotic response and not consequence of salt-specific stimulation. (uni-marburg.de)
activation is via the proximal promoter. (lu.se)

Moreover, an HDAC3 binding-deficient SMRT DAD mutant coactivated p53 transcriptional activity. (nih.gov)
We identified BDNF as an important target of Sirt1 and TORC1 transcriptional activity in normal and HD neurons. (harvard.edu)
Genetic or pharmacologic modulation of eNOS expression and activity resulted in reciprocal conversion of the transcriptional signature in cells from patients with bad or good outcome, respectively, highlighting the relevance of eNOS in PCa progression. (tcd.ie)
This activation appears to be specific to osteoblasts, since progression to a myoblast phenotype results in no change in reporter gene activity. (umn.edu)
Figure 2: Ldh activity increases during HFSC activation. (nature.com)
NK-cell activation is associated with increased HIV transcriptional activity following allogeneic hematopoietic cell transplantation. (harvard.edu)
Furthermore, overexpression of ZNF322 in COS-7 cells activates the transcriptional activity of SRE and AP-1. (anthropogeny.org)
and exerts a dominant negative effect on the transcriptional activity of hGRα. (medscape.com)

Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline). (wikipedia.org)
Whereas in the acute phase of the disease immunoregulatory processes prevail in the hippocampus and the cortex, we observed a strong activation of neurogenic processes in the hippocampal dentate gyrus, both by gene expression and immunohistology starting as early as 3 days after infection. (biomedcentral.com)
Effect of AhR activation on STAT1, cell-cycle and gene expression in macrophages. (elifesciences.org)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)

Transcriptional Control of Regulatory T Cells in Cancer: Toward Therapeutic Targeting? (mdpi.com)

Culturing these preosteoblast cells in the absence of critical co-factors results in an inhibition of differentiation and leads to a delayed induction of OST transcripts as well as the attenuation of transcriptional activation. (umn.edu)
These results show that the murine OST gene is regulated at the transcriptional level in an osteoblast-specific, differentiation-dependent manner during the differentiation of MC3T3 osteoblasts. (umn.edu)
Comparing these transcriptional responses provides a greater understanding of the mechanisms cells use in the differentiation between types of DNA damage and the potential consequences of different sources of damage. (biomedcentral.com)
The excessive activation of AhR resulted in neural differentiation of Neuro2a cells. (biomedcentral.com)

Endothelial NOS, estrogen receptor beta, and HIFs cooperate in the activation of a prognostic transcriptional pattern in aggressive human prostate cancer. (tcd.ie)
Echinacoside-Zinc Nanomaterial Inhibits Skin Glycation by Suppressing the Transcriptional Activation of the Receptor for Advanced Glycation End-Products. (bvsalud.org)

Surprisingly, PITX2A activation of the ANF and PLOD1 promoters is repressed by co- transfection of Nkx2.5 in the C3H10T1/ 2 embryonic fibroblast cell line. (lu.se)

The neurological properties of N2a-Rα based on AhR activation were evaluated by immunohistochemical analysis of cytoskeletal molecules and by RT-PCR analysis of mRNA expression of neurotransmitter-production related molecules, such as tyrosine hydroxylase (TH). (biomedcentral.com)

In vitro monocyte-derived macrophages (MoMFs) were assessed for activation and cytokine production. (imperial.ac.uk)
This macrophage polarization can be simulated in vitro using lipopolysaccharide (LPS) and interferon (IFN)-γ stimulation for a proinflammatory subset termed classical activation and stimulation with interleukin (IL)-4 and IL-13 for an alternative polarization phenotype. (haematologica.org)
Although the signaling mechanisms governing myelination are not fully understood, NF-κB activation in Schwann cells has been implicated as a key regulator in vitro . (jneurosci.org)

2014). Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. (cdc.gov)
These genome wide transcriptional responses are very tightly regulated and complex. (biomedcentral.com)
While it is known that genotoxic agents, such as IR, activate transcriptional programs involved in maintaining the integrity of the genome, we also want to investigate whether or not the genotoxic breaks could affect lymphocyte-specific maturation transcriptional responses similar to those we observed following RAG-induced physiological DSBs. (biomedcentral.com)

Aberrant vascular remodeling contributes to the progression of many aging-associated diseases, including idiopathic pulmonary fibrosis (IPF), where heterogeneous capillary density, endothelial transcriptional alterations and increased vascular permeability correlate with poor disease outcomes. (researchgate.net)

Conclusions: CPI-Hep is associated with activation of peripheral monocytes and an enhanced cytotoxic, effector CD8+ T cell phenotype. (imperial.ac.uk)

The blood microvascular endothelium consists of a heterogeneous population of cells with regionally distinct morphologies and transcriptional signatures in different tissues and organs. (researchgate.net)

In addition, its expression is considered to be more stable in eukaryotic cells due to being human codon optimized and utilizing three minimal transcriptional activation domains. (wikipedia.org)
Effects of AhR activation in PBMC and purified CD4+ T cells. (elifesciences.org)
Using a mouse model, we show that nuclear factor κB activation in Schwann cells is not required for myelination in vivo . (jneurosci.org)
The activation of exogenous AhR in N2a-Rα cells was confirmed using RNAi, with si-AhR suppressing the expression of exogenous AhR. (biomedcentral.com)
N2a-Rα cells exhibited constant activation of the exogenous AhR. (biomedcentral.com)

WC-Co treatment also increased transcriptional activation of AP-1, NF-?B, and VEGF by reporter assay. (cdc.gov)

These studies suggest a key role of Sirt1 in transcriptional networks in normal and HD brain and offer an opportunity for therapeutic development. (harvard.edu)
Germline nuclear RNAi antagonizes this temperature effect at the transcriptional level and therefore may play a key role in heat stress response in C. elegans . (biomedcentral.com)

Mechanistic studies at the cellular level showed that MDM2 can interact with STAT2 to form a transcriptional complex and thus promote RAGE transcriptional activation . (bvsalud.org)

EGCG significantly inhibited phosphorylation of the MAPKs p38 and c-Jun N-terminal kinase (JNK), and NFκB and AP-1 transcriptional activities. (molvis.org)

To understand if polarization of macrophages can lead to a procoagulant macrophage subset we polarized human monocyte derived macrophages to a proinflammatory and an alternative activation state. (haematologica.org)
AhR activation inhibits HIV-1 replication in macrophages. (elifesciences.org)
Reduced CDK/cyclin, phospho-SAMHD1 and dTNP levels in macrophages following activation of AhR. (elifesciences.org)

Transcriptional profiling indicated the presence of activated monocyte and enhanced effector CD8+ T cell populations in CPI-Hep. (imperial.ac.uk)

It inhibited the function of the MDM2/STAT2 complex and suppressed the transcriptional activation of RAGE , thereby exerting antiglycation effects. (bvsalud.org)

We identified a central lymphocyte-specific transcriptional response common to both physiologic and genotoxic breaks, which includes many lymphocyte developmental processes. (biomedcentral.com)

Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma. (bioseek.eu)

Here, we show that NF-κB activation in SCs is dispensable for myelination in vivo . (jneurosci.org)

These results suggest that PXR is trapped in paraspeckles and that the activation of PXR by its ligands facilitates its disassociation from paraspeckles. (aspetjournals.org)

Figure 6: Stimulation of Myc levels promotes HFSC activation. (nature.com)
Previous polarization to either a proinflammatory or an alternative activation subset does not change the subsequent stimulation of TF. (haematologica.org)

Effect of AhR activation on HIV-1 replication. (elifesciences.org)
This study focused on the effect of AhR activation in the developing neuron. (biomedcentral.com)

It is also human codon optimized and composed of three minimal VP16 activation domains. (wikipedia.org)

Here we compare transcriptional responses to physiological DNA breaks with responses to genotoxic DNA damage induced by ionizing radiation. (biomedcentral.com)
By comparing the transcriptional responses to both types of DNA damage, we can compare the similarities in the responses to damage as well as the differences induced by genotoxic damage. (biomedcentral.com)

We previously showed that physiologic breaks initiate lymphocyte development-specific transcriptional programs. (biomedcentral.com)

Anatomy14

Organisms5

Diseases1

Chemicals and Drugs145

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes70

Information Science3

Regulation10

Repressor5

Repression2

Chromatin4

Activator3

Genes5

Regulators3

Post-transcriptional1

Corepressor2

Regulates2

Endogenous4

Pathways1

Transactivation3

Protein3

Promoter2

Activity8

Gene expression4

Regulatory1

Differentiation4

Receptor2

Transfection1

Cytoskeletal1

Vitro3

Genome3

Alterations1

Conclusions1

Distinct1

Cells5

Reporter1

Role2

Level1

Kinase1

Macrophages3

Monocyte1

Function1

Response1

Complex1

Vivo1

Results1

Stimulation2

Effect2

Human1

Damage2

Specific1