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  • inducible
  • Tetracycline-Controlled Transcriptional Activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline). (wikipedia.org)
  • cell's
  • To initiate the transcription process in a cell's nucleus, DNA double helices are unwound and hydrogen bonds connecting compatible nucleic acids of DNA are broken to produce two unconnected single DNA strands. (wikipedia.org)
  • loci
  • Activation-induced deaminase (AID) can drive lymphomagenesis by generating off-target DNA breaks at loci that harbor highly active enhancers and display convergent transcription. (elifesciences.org)
  • Evidence from various studies has shown that genetic susceptibility to T2DM is polygenic [ 5 , 6 ], and Genome Wide Association Studies (GWAS) have reported more than 20 genetic loci associated with the risk of T2DM [ 7 , 8 ]. (hindawi.com)
  • Complex haploinsufficiency, which occurs when a strain containing two heterozygous mutations at distinct loci shows a phenotype that is distinct from either of the corresponding single heterozygous mutants, is an expedient approach to genetic interactions analysis in diploid organisms. (g3journal.org)
  • introns
  • must be replaced by that of a transcription unit containing regions which will be lost from the mature messenger - which I suggest we call introns (for intragenic regions) - alternating with regions which will be expressed - exons. (wikipedia.org)
  • However, different types of introns were identified through the examination of intron structure by DNA sequence analysis, together with genetic and biochemical analysis of RNA splicing reactions. (wikipedia.org)
  • Following transcription into RNA, group I and group II introns also make extensive internal interactions that allow them to fold into a specific, complex three-dimensional architecture. (wikipedia.org)
  • assay
  • To determine the extent of genetic variations and the nature of swine HEV infections in U.S. pigs, we developed a universal reverse transcription-PCR (RT-PCR) assay that is capable of detecting genetically divergent strains of HEV. (pubmedcentralcanada.ca)
  • involves
  • Within the last decade, studies have revealed that abortive initiation involves DNA scrunching, in which RNA polymerase remains stationary while it unwinds and pulls downstream DNA into the transcription complex to pass the nucleotides through the polymerase active site, thereby transcribing the DNA without moving. (wikipedia.org)
  • interactions
  • Finally, we characterized the genetic interactions of a set of biofilm related TFs in the first two steps of biofilm formation, adherence and filamentation of adherent cells. (g3journal.org)
  • Initiation
  • After each nucleotide-addition step in initial transcription, RNA polymerase, stochastically, can proceed on the pathway toward promoter escape (productive initiation) or can release the RNA product and revert to the RNA polymerase-promoter open complex (abortive initiation). (wikipedia.org)
  • It had been observed that RNA polymerase did not escape from the promoter during transcription initiation, so it was unknown how the enzyme could read the DNA strand to transcribe it without moving downstream. (wikipedia.org)
  • mechanism
  • It is involved in controlling both the sleep-wake cycle and circadian regulation of gene expression by promoting transcription in a negative feedback mechanism. (wikipedia.org)
  • interaction
  • Because of the largely diploid nature of the human fungal pathogen Candida albicans , genetic interaction analysis has been limited to a small number of large-scale screens and a handful for gene-by-gene studies. (g3journal.org)
  • Here, we describe the construction of a barcoded-library of 133 heterozygous TF deletion mutants and deletion cassettes for designed to facilitate complex haploinsufficiency-based genetic interaction studies of the TF networks in C. albicans . (g3journal.org)
  • The genetic interaction networks at each stage of biofilm formation are significantly different indicating that the network is not static but dynamic. (g3journal.org)
  • One area of C. albicans genetic analysis that has not been extensively developed compared to other systems is genetic interaction analysis. (g3journal.org)
  • activation
  • Arrhenius graphs for both these rates are given, and the activation energies mtieasured are 11,000 calories per mole for decay and 22,000 calories per mole for transcription. (sciencemag.org)
  • yield
  • A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. (wikipedia.org)
  • cells
  • If cells are irradiated shortly after induction, the transcription of the DNA ceases, and the enzyme produced by the messenger RNA is observed to reach a maximum. (sciencemag.org)
  • We present signal feature analysis to detect vulnerable regions and quantified from human cells how convergent transcription contributes to R-loop generation and RNA polymerase stalling. (elifesciences.org)
  • Cancerous cells often contain alterations to the genetic information in their DNA. (elifesciences.org)
  • Several researchers have sequenced the entire DNA of childhood leukemia cells, with the result that almost all of the genetic alterations linked to these conditions have been catalogued. (elifesciences.org)
  • Research
  • This discovery of endogenous R-loops, in conjunction with rapid advances in genetic sequencing technologies, inspired a blossoming of R-loop research in the early 2000s that continues to this day. (wikipedia.org)
  • cell
  • TRANSCRIPTION - The cell makes a copy of the gene whose message is to be interpreted. (thehumangenome.co.uk)
  • Moreover, this clock was reconstructed in a test tube (i.e., in the absence of any cell components), proving that accurate 24-hour clocks can be formed without the need for genetic feedback circuits. (wikipedia.org)
  • lead
  • This would explain how pieces of DNA might be lost, added, or moved to cause the genetic errors that lead to leukemia. (elifesciences.org)
  • Previous reports demonstrated that the MFNLP could lead to the duplication of transcription factor binding sites (TFBS) enhancing the activity of the HIV-1 subtype C LTR. (mdpi.com)
  • CWO is a transcriptional repressor and antagonistic competition between CYC-CLK and CWO lead to control of E-box mediated transcription. (wikipedia.org)
  • allows
  • Enclosed within the envelope is a capsid surrounding single stranded RNA, integrase, protease, and reverse transcriptase, an enzyme that allows for the reversal of genetic transcription. (wikipedia.org)
  • structure
  • The members of the MADS-box transcription factor gene family are organised into a number of sub-groups depending on their domain structure. (uio.no)