• Membrane-bound HER receptors activate numerous tumor promoting signaling cascades via this mechanism, including the PI3K/AKT, Ras/Raf/Mek/Erk, PLCγ/PKC, and signal transducer and activator of transcription (STAT) pathways [1] , [2] . (plos.org)
  • The basic region is involved in RNA (TAR, trans-activation response element) binding and Tat proteins thus belong to the family of arginine-rich motif (ARM) RNA binding proteins. (wikipedia.org)
  • Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)
  • Specifically, as they wanted to analyze this interaction in human cells, they decided to utilize nuclease-inactive dCas9-based transcriptional activators where endogenous gene activation can serve as a sensitive measure of dCas9 binding to target DNA. (genomeweb.com)
  • Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)
  • [Show more] their performance in the Transcriptional Activator-Facilitated Enhancer trap (TAFET) constructs to induce cell- or tissue-specific expression patterns in rice. (edu.au)
  • The GAL4/VP16 TAFET system was able to reveal varying levels of tissue specific expression patterns in rice in the vegetative and generative tissues, and the expression patterns were proven to be due to the activity of the GAL4/VP16 transcriptional activator. (edu.au)
  • NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. (thermofisher.com)
  • The ZEBRA (Z EBV replication activator) protein is the major transcription factor of EBV, expressed upon EBV lytic cycle activation. (mdpi.com)
  • The BOB.1 / OBF.1 / OCA-B protein (henceforth designated as BOB.1 / OBF.1) is a B cell-specific co-activator of the Oct1 and Oct2 transcription factors. (nih.gov)
  • Like other lentiviruses, HIV-1 encodes a trans-activating regulatory protein (Tat), which is essential for efficient transcription of the viral genome. (wikipedia.org)
  • In addition, shrinkage of the dentate gyrus occurs in the CaMKIIα-tTA activator line, although it is not yet known whether this is due to the expression of tTA or to the disruption of one or more endogenous mouse genes. (alzforum.org)
  • Inside the nucleus, HER family members (EGFR, HER2, and HER3) have been shown to function as co-transcriptional activators for various cancer-promoting genes. (plos.org)
  • These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
  • rTg4510 mice ("r" for regulatable) are produced by crossing the 4510 responder line, carrying a human MAPT P301L cDNA downstream of a tetracycline operon-responsive element (TRE), to an activator line expressing a tetracycline-controlled transactivator (tTA) under control of the CaMKIIα promoter. (alzforum.org)
  • T2 mice were then crossed with the CaMKIIα-tTA activator line to create bi-transgenic mice (designated rT2/T2 when homozygous for the MAPT P301L transgene). (alzforum.org)
  • Cardiac malformations, adrenal agenesis, neural crest defects and exencephaly in mice lacking Cited2, a new Tfap2 co-activator. (infrafrontier.eu)
  • The activity of these XCI-activators is counterbalanced by autosomally encoded XCI-inhibitors, many of which are pluripotency factors involved in repression of Xist , providing a powerful link between loss of pluripotency and XCI initiation. (erasmusmc.nl)
  • Tat acts by binding to an RNA stem-loop structure, the trans-activation response element (TAR), found at the 5′ ends of nascent HIV-1 transcripts. (wikipedia.org)
  • Transfecting sgRNA variants and a dCas9-P300 transactivator into human cells, we observed that hp-sgRNAs can tune gene activation at the target locus, suggesting modulation of dCas9 binding. (genomeweb.com)
  • The lack of activity is a direct consequence of lack of the co-activator. (nih.gov)
  • We have developed a functional "no-hybrids" screen in the fission yeast Schizosaccharomyces pombe based on the transcription transactivator activity of human p53. (ox.ac.uk)
  • In its paper published in Nature Biotechnology , the team demonstrated that designed hp-sgRNAs can tune the activity of a transactivator based on Cas9 from Streptococcus pyogenes (SpCas9). (genomeweb.com)
  • A "no-hybrids" screen for functional antagonizers of human p53 transactivator function: dominant negativity in fission yeast. (ox.ac.uk)
  • Thus, the ability for HER3 to function as a transcriptional co-activator may be dependent on specific C-terminal TADs. (plos.org)
  • While the classical membrane-bound functions of HER family RTKs have been extensively studied, accumulating data suggest that these receptors can be found in the cell's nucleus where they can function as co-transcriptional activators [4] , [5] . (plos.org)
  • Activator appliances change the function and the position of the mandible in order to transmit forces to the dentition and the basal bone1. (bvsalud.org)
  • Our work involving studies of tetraploid, triploid and diploid mouse ES cells revealed that initiation of XCI is a stochastic process, and indicated the presence of X-linked activators of XCI. (erasmusmc.nl)
  • Our present research is aimed at identifying novel XCI-activators, elucidate the mechanisms by which they direct female exclusive XCI, and translate these findings to human. (erasmusmc.nl)
  • The purpose of this study was to evaluate the influence of the vertical and horizontal dimensions of the construction bite in the dentoskeletal changes induced by Klammt Open Elastic Activator during the treatment of Class II division 1. (bvsalud.org)
  • Like other lentiviruses, HIV-1 encodes a trans-activating regulatory protein (Tat), which is essential for efficient transcription of the viral genome. (wikipedia.org)
  • TAT, which stands for trans-activator of transcription, contains 86 amino acid residues in its sequence. (proteopedia.org)
  • The nucleotide-binding domain and leucine-rich repeat receptor family members CIITA and NLRC5 function as the major transcriptional activators of MHC class II and class I gene expression, respectively. (nih.gov)