Streptomyces lividans. Medical search. Frequent questions

The effects of tylosin-related macrolide antibiotics were examined in cell-free protein synthesis (using a coupled transcription-translation system derived from Streptomyces lividans) and against whole cells of that organism. (lancs.ac.uk)

By sequence similarity analysis and functional assays from the species Streptomyces lividans TK23, S. coelicolor A3(2) and S. glaucescens GLA.O, first hints for a widespread lack of MalP and presence of AmlE in the class Actinobacteria were given. (frontiersin.org)

b The growth curves of S. lividans TK24 carrying pIJ486 expressing SP Vsi -CelA in the indicated media expressed as values of dry cell weight. (biomedcentral.com)
c The dry cell weight in gram per liter produced by S. lividans TK24 with pIJ486 expressing SP Vsi -CelA in the different media (as in Fig. 1 b) at the indicated growth phases. (biomedcentral.com)
d The amount of CelA secreted (mg) correlated to a gram of dry cell mass by S. lividans TK24 with pIJ486 expressing SP Vsi -CelA in the different media (as in Fig. 1 b) for the indicated time related to its growth curves in the same media. (biomedcentral.com)
Here we report the development of a robust gene deletions system for Streptomyces lividans TK24 based on a BAC library combined with the λ-Red recombination technique. (figshare.com)
The developed system was validated by systematically deleting the most highly expressed genes encoding alternative sigma factors and several other regulatory genes within the chromosome of S. lividans TK24. (figshare.com)

The structures of cofactor-free haloperoxidases from Streptomyces aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been determined at resolutions between 1.9 A and 1.5 A. The structures of two enzymes complexed with benzoate or propionate identify the binding site for the organic acids which are required for the haloperoxidase activity. (rcsb.org)

In S. lividans the GlxA gene is part of the cslA/glxA operon that contains genes encoding putative enzymes involved in glycan processing. (bl.uk)
Several independent gene clusters containing varying lengths of type I polyketide synthase genes were isolated from 'Streptomyces nanchangensis' NS3226, a producer of nanchangmycin and meilingmycin. (microbiologyresearch.org)
The presence of erm type I or erm type II resistance genes in S. lividans markedly affected the resistance phenotype and studies involving ribosomes from such strains revealed differences in macrolide activity that were not otherwise apparent. (lancs.ac.uk)

2) pSET152, an Escherichia coli plasmid with ϕC31 attP site, and pHZ1358, a Streptomyces-Escherichia coli shuttle cosmid vector, both carrying oriT from RP4, can be mobilized from E. coli into NS3226 by conjugation. (microbiologyresearch.org)
Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. (microbiologyresearch.org)

1) Streptomyces phage ϕC31 and its derived vectors can infect and lysogenize this strain. (microbiologyresearch.org)

Streptomyces are filamentous soil-dwelling bacteria with a complex life cycle. (bl.uk)
However, DnaA proteins from two Gram-positive bacteria, Bacillus subtilis and Streptomyces lividans , while capable of specifically interacting with the DnaA box sequences at oriV , do not bind stably and fail to induce open complex formation. (sri.com)

Additionally, a Streptomyces lividans enzyme, GlxA, which is distantly related to AA5, has been shown to oxidize glycolaldehyde and a deletion mutant showed a loss of glycan accumulation at hyphal tips [ 17 ]. (cazypedia.org)

Streptomyces cacaoi is a bacterium species from the genus of Streptomyces. (wikipedia.org)

A structural similarity search indicated the highest structural similarity to an esterase from Streptomyces scabies, followed by GDSL family enzymes. (rcsb.org)
Structural and Kinetic Analysis of the Streptomyces Lividans Xylanase 10A. (expasy.org)
In contrast, uptake of the drugs into S. lividans was influenced, either positively or negatively, by each of these structural parameters. (lancs.ac.uk)

polR, a pathway-specific transcriptional regulatory gene, positively controls polyoxin biosynthesis in Streptomyces cacaoi subsp. (wikipedia.org)
Analysis of the simocyclinone biosynthesis ( sim ) gene cluster of Streptomyces antibioticus Tü6040 led to the identification of a putative pathway specific regulatory gene simReg1 . (springeropen.com)

Characterization of the Polyoxin Biosynthetic Gene Cluster from Streptomyces cacaoi and Engineered Production of Polyoxin H". Journal of Biological Chemistry. (wikipedia.org)

For the industrially used strain S. lividans this switch is dependent on the bioavailability of copper (Cu) in the environment. (bl.uk)
The reported here gene deletion system will further facilitate work on S. lividans strain improvement as a host for either secondary metabolites or protein production and will contribute to basic research in streptomycetes physiology, morphological development, secondary metabolism. (figshare.com)

In the case of Streptomyces a development switch occurs with the concomitant production of secondary metabolites, many of which have pharmaceutical properties. (bl.uk)