• We use a combination of MS techniques, namely liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) on a triple quadrupole, for the identification of the polyphenolic constituents of the soluble fraction of GADF. (unboundmedicine.com)
  • Subsequently, phosphatidylcholine, lysophosphatidyl¬choline, sphingo-myelin, ceramide, phosphatidylethanolamine, phosphatidylethanolamine-based plasmalogen, cholesterol and cholesteryl ester content of the separated lipoproteins was quantified by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Results: Analysis of FPLC fractions with polyacrylamide gel electrophoresis demonstrated that albumin partially co-elutes with HDL fractions. (uni-regensburg.de)
  • We describe features of tandem mass spectra of lithiated adducts of triacylglycerol (TAG) species obtained by electrospray ionization mass spectrometry (ms) with low-energy collisionally activated dissociation (CAD) on a triple stage quadrupole instrument. (wustl.edu)
  • Schemes are proposed to rationalize formation of major fragment ions in tandem mass spectra of lithiated TAG that are supported by studies with deuterium-labeled TAG and by source CAD-MS 2 experiments. (wustl.edu)
  • Nano-flow liquid chromatography tandem mass spectrometry (nano-flow LC-MS/MS) is the mainstay in proteome research because of its excellent sensitivity but often comes at the expense of robustness. (nature.com)
  • 120,000 peptides in 16 h and sample multiplexing using tandem mass tags increases throughput to 11 proteomes in 16 h. (nature.com)
  • The analyte ions of interest were guided into an ion trap mass spectrometer for tandem mass analysis. (elsevierpure.com)
  • Tandem mass spectrometry was used to confirm the identification of important compounds in each aerosol drug sample. (elsevierpure.com)
  • Our data demonstrate that nanoEESI tandem mass spectrometry is a fast and sensitive technique for the analysis of aerosol drug preparations, showing promising applications in pharmacology studies and in situ analysis of aerosol drugs on the market. (elsevierpure.com)
  • Using the quadrupole time-of-flight (QTOF) mass spectrometer, the presence of cocaine and impurities detected were confirmed by accurate tandem MS data. (edu.au)
  • The monomer and dimer structures of the amyloid fragment Aβ(1-16) sequence formed in H 2 O were investigated using electrospray ionization mass spectrometry (MS) and tandem MS (MS/MS). Aβ16 monomers and dimers were indicated by signals representing multiple proton adduct forms, [monomer+zH] n+ (=M z+ , z = charge state) and [dimer+zH] z+ (=D z+ ), in the MS spectrum. (accesson.kr)
  • The common derivatization method of primary amino groups by 2,4,6-trinitrobenzene sulfonate (TNBS) was applied to the detection of free adducts of advanced glycation end-products (AGEs), in combination with liquid chromatography-tandem mass spectrometry-multiple reaction monitoring (LC-MS/MS-MRM). (elsevierpure.com)
  • An ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-MS/MS) method was developed for the analysis of steroids and their glucuronides in urine samples. (vtt.fi)
  • Addition of 0.1% CYMAL-5 (final concentration) during trypsin treatment was compatible with both matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis, and gave a better digestion efficiency than n-octylglucoside, which we previously reported. (nih.gov)
  • Tandem quadrupole mass spectrometry HPLC-ESI-MS/MS CID-MS/MS Multiple reaction monitory (MRM) Quercetin Apigenin Kaempferol a b s t r a c t The presence of three flavonols (quercetin, kaempferol, myricetin) and two flavones (apigenin, luteolin) were investigated in the extract of fresh spinach leaves. (utc.fr)
  • Furthermore, the results were confirmed by HPLC coupled to an electrospray ionisation tandem triple-quadrupole mass spectrometer ESI-MS performing low-energy collision induced dissociation (CID-MS/MS) in the collision cell (HPLC-ESI-MS/MS). Analyses were made in the multiple reaction monitory (MRM) mode. (utc.fr)
  • Detection and quantification are done using negative-ion TurboIonSpray ionization, a variant of electrospray ionization, tandem mass spectrometry. (cdc.gov)
  • Secondary electrospray ionization mass spectrometry (SESI-MS) enables the detection of volatile organic compounds (VOCs) without the need for any sample pretreatment. (jove.com)
  • Using native mass spectrometry, we identify the stoichiometry of sub-complexes and monitor oligomerisation of various assemblies. (nature.com)
  • Once ionized, the volatiles are pulled into the mass spectrometer for analysis (4). (jove.com)
  • Choose the appropriate vessel for growing your cultures, considering the growth requirements of the species in your experiment (e.g., aeration, light, temperature, etc.), as well as the efficient delivery of volatiles to the mass spectrometer. (jove.com)
  • Finally, most of the identities were unequivocally confirmed by accurate mass measurements on the TOF spectrometer. (unboundmedicine.com)
  • Integrate capillary electrophoresis (CE) and electrospray ionization (ESI) into a single microfluidic device to rapidly prepare, separate, and electrospray biological samples directly into your Thermo Scientific mass spectrometer . (thermofisher.com)
  • Analytes are electrosprayed directly into the mass spectrometer through the ESI emitter on the corner of the microfluidic chip. (thermofisher.com)
  • Mass measurements were run on a hybrid quadrupole time of flight mass spectrometer Q-Tof Premier (Waters Micromass, Manchester, UK), equipped with an orthogonal Z-spray electrospray (ESI) interface. (hindawi.com)
  • Subsequently, PMA alone was routinely analyzed using an Agilent 1100 LC/MSD SL mass spectrometer system using selected ion monitoring (SIM) in the negative ion ESI LC/MS mode for maximum sensitivity. (cdc.gov)
  • An LC-MS/MS SCIEX API4000 instrument, furnished with a positive electrospray ionization source and HPLC of Shimadzu, consisted of an SIL-HTC autosampler, dual pump, and column oven, which were utilized in the present work. (japsonline.com)
  • Sensitive, selective and reliable high-performance liquid chromatography (HPLC)-diode array detection (DAD)/electrospray ionization multi-stage mass spectrometry (ESI-MS n ) methods have been developed for the characterization of nine 2-glucosyloxycinnamic acid derivatives and quantitative analysis of three of the major 2-glucosyloxycinnamic acids, cis -melilotoside, trans -melilotoside and dihydromelilotoside, present in Dendrobium medicinal plants. (edu.hk)
  • Electrospray ionization-ion trap mass spectrometry for structural analysis of complex N-linked glycoprotein oligosaccharides. (consensus.app)
  • Electrospray ionization-ion trap mass spectrometry effectively reveals the structural characterization of complex N-linked glycoprotein oligosaccharides, enabling detailed analysis of their linkages and structures. (consensus.app)
  • Electrospray ionization-ion trap mass spectrometry, with its capacity to perform multiple stages of fragmentation (MSn), is demonstrated as an effective method for the structural characterization of permethylated N-linked complex glycoprotein oligosaccharides. (consensus.app)
  • Using ion trap mass spectrometry, removal of these substituents permits determination of branching patterns and intersaccharide linkages by MS3 and MS4. (consensus.app)
  • Higher-order experiments further illustrate the potential of electrospray ionization-quadrupole ion trap mass spectrometry for carbohydrate analysis, as MS8 is used to produce significant and otherwise unobtainable branching information for an oligosaccharide from chicken ovalbumin. (consensus.app)
  • These studies constitute further evidence of the unique role that ion trap mass spectrometry can assume in the area of oligosaccharide analysis. (consensus.app)
  • An optimized method using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) in negative ion mode has been developed for screening different structural classes of intact glucosinolates in six Chinese medicinal herbs. (unboundmedicine.com)
  • This paper describes the use of electrospray ionization-mass spectrometry (ESI-MS) to screen two libraries of soluble compounds to search for tight binding inhibitors for carbonic anhydrase II (EC 4.2.1.1). (figshare.com)
  • Furthermore, we identified a total of 32 component compounds via chromatography and mass spectrometry. (hindawi.com)
  • Desorption electrospray ionization - mass spectrometry (DESI-MS) is a useful technique for the qualitative analysis of compounds found in seized drug material. (edu.au)
  • Straight-forward mass characterization of the complex mixtures could be achieved. (degruyter.com)
  • The rapid and highly sensitive atmospheric-pressure ionization MS with electrospray ionization analysis technique proposed here is well suited for direct characterization of sucrose esters. (degruyter.com)
  • The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
  • Desorption electrospray ionization mass spectrometry (DESI-MS) is a powerful method for generating ions at ambient conditions without the need for sample preparation or pretreatment. (nova.edu)
  • These results suggest that a charged droplet pick-up mechanism is responsible for desorption and ionization of liquid samples by DESI. (njit.edu)
  • Dive into the research topics of 'Direct Analysis of Liquid Samples by Desorption Electrospray Ionization-Mass Spectrometry (DESI-MS)'. Together they form a unique fingerprint. (njit.edu)
  • Direct Analysis of Liquid Samples by Desorption Electrospray Ionization-Mass Spectrometry (DESI-MS) . Journal of the American Society for Mass Spectrometry , 20 (1), 10-19. (njit.edu)
  • Qualitative analysis of seized cocaine samples using desorption electrospray ionization- mass spectrometry (DESI-MS) . Drug Testing and Analysis , 7 (5), 393-400. (edu.au)
  • This paper describes a multifunctional semi-closed droplet-array chip coupled with electrospray ionization mass spectrometry ( ESI-MS ) detection for multiple sample pretreatment and analysis. (rsc.org)
  • 1000 for methylglyoxal-derived hydroimidazolone) with a detection limit of 1.0 nM (10 fmol/injection volume), which was due to the high ionization efficiency of the derived trinitrophenol moiety and its hydrophobicity. (elsevierpure.com)
  • To test this hypothesis, we used a rat model of endurance exercise, a microwave irradiation for accurate detection of glycogen in the brain (the cortex, hippocampus, and hypothalamus), and capillary electrophoresis mass spectrometry-based metabolomics to observe the comprehensive metabolic profile of the blood. (frontiersin.org)
  • The spectra are collected in positive ion mode using 0.1 % formic acid, 5.0% methanol, and 94.9 % water (v/v) as the electrospray solution, delivered at 5 nL/s through a non-conductive silica capillary with a sharpened needle tip (40 μm ID). (jove.com)
  • The spectra distinguish isomeric triacylglycerol species and permit assignment of the mass of each fatty acid substituent and positions on the glycerol backbone to which substituents are esterified. (wustl.edu)
  • Nevertheless, Girard P derivatives of bile alcohols and C27 acids give an intense molecular ion ([M]+) upon electrospray ionization and informative fragmentation spectra. (swan.ac.uk)
  • The identities of the latter three major 2-glucosyloxycinnamic acids were confirmed by comparing their retention times, UV and mass spectra with those of the reference standards. (edu.hk)
  • Sequential window acquisition of all theoretical fragment ion spectra (SWATH) is a label-free mass spectrometry quantitative technique, which can retain complete fragment ion data and has good accuracy as well as reproducibility. (ncl.edu.tw)
  • Enzyme-assisted derivatization for sterol analysis (EADSA) is a technology designed to enhance sensitivity and specificity for sterol analysis using electrospray ionization-mass spectrometry. (swan.ac.uk)
  • An L-shaped capillary was adopted as the interface of the droplet array and ESI-MS , using its inlet end as a sampling probe for droplets and its outlet with a tip size of ∼20 μm as an electrospray emitter, without the need for any droplet extraction device. (rsc.org)
  • Without any sample pretreatment, aerosol drugs were manually sprayed into the primary ion plume created by a nano electrospray emitter for direct ionization under ambient conditions. (elsevierpure.com)
  • Inset: The SESI electrospray needle is a silica capillary (40 μm ID) with a sharpened needle tip. (jove.com)
  • In this webinar, the benefits of integrating cutting-edge microfluidic technology with capillary electrophoresis (CE) and electrospray ionization (ESI) for enhanced mass spectrometry (MS) analysis is presented. (thermofisher.com)
  • A novel sensitive method utilizing nano extractive electrospray ionization mass spectrometry (nanoEESI-MS) has been developed for the rapid analysis of aerosol drug samples with quantitative information. (elsevierpure.com)
  • A specific liquid chromatography-mass spectrometry/mass spectrometry spectrometric procedure for the quantitation of amprenavir drug in biological matrices was developed and validated. (japsonline.com)
  • Tracqui A, Kintz P, Ludes B, Rouge C, Douibi H, Mangin P. High-performance liquid chromatography coupled to ion spray spectrometry for the determination of colchicine at ppb levels in human biofluids. (cdc.gov)
  • Determination of colchicine in biological fluids by liquid chromatography mass spectrometry with pneumatically assisted electrospray ionization. (cdc.gov)
  • SESI ionization occurs by proton transfer reactions between the electrospray solution and the volatile analyte, and is therefore suitable for the analysis of hetero-organic molecules, just as in traditional electrospray ionization (ESI). (jove.com)
  • Mass spectrometry coupled to liquid chromatography is a powerful tool for the analysis of complex plant derivatives such as GADF. (unboundmedicine.com)
  • These results demonstrate that pDESI-MS offers the potential to transform current approaches for implementing spray-based ambient ionization techniques in chemical analysis and imaging. (nova.edu)
  • The results indicate the atmospheric-pressure ionization MS with electrospray ionization analysis is an effective method for the analysis of sucrose esters composed of different isomers. (degruyter.com)
  • This strategy has been successfully from large groupings of organisms, electrospray ionization used with consensus 16S ribosomal RNA primers for deter- mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to mining bacterial diversity, both in environmental samples identify organisms in a sample. (cdc.gov)
  • It was isolated and characterized as Na2[WVI2O6(C4O3NH10)2]·6H2O by single-crystal and powder X-ray diffraction, FT-IR spectroscopy, thermogravimetrical analysis (TGA), and elemental analysis in solid state and by electro-spray ionization mass spectrometry (ESI-MS), 13C, and 183W NMR, as well as Raman spectroscopy in solution. (lu.se)
  • Also, unlike in electrospray ionization (ESI), inhibition of electrochemical reduction in the negative ion mode was observed for liquid sample DESI. (njit.edu)
  • The literature on amprenavir unveiled that there are few analytical procedures available for the estimation of drug in API and dosage forms on liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) with more retention times. (japsonline.com)
  • Therefore, we developed a method for measuring bisphenol A (BPA) and bisphenol A diglycidyl ether (BADGE) metabolites in human blood samples using high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-MS). Human blood samples were processed using enzymatic deconjugation of the glucuronides followed by a novel sample preparation procedure using a solid-phase-cartridge column. (who.int)
  • 2000) describe a method for determining perchlorate in urine samples using electrospray ionization mass spectrometry. (cdc.gov)
  • This ionization technique can be carried out in natural and uneven biological surface. (wikipedia.org)
  • As the volatiles traverse the SESI reaction chamber they pass through the electrospray cloud and become ionized (3). (jove.com)
  • The SESI reaction chamber was custom built and fitted to an API-3000 (SCIEX), replacing the original electrospray ion source. (jove.com)
  • Because molecules and aerosols are ionized by interacting with an electrospray plume, LAESI's ionization mechanism is similar to SESI and EESI techniques. (wikipedia.org)
  • Laser ablation electrospray ionization (LAESI) is an ambient ionization method for mass spectrometry that combines laser ablation from a mid-infrared (mid-IR) laser with a secondary electrospray ionization (ESI) process. (wikipedia.org)
  • LC conditions for chromatographic separation were coupled with electrospray "soft ionization" and mass spectrometry. (cdc.gov)
  • Commercial and synthesized sucrose fatty acid esters were qualitatively analyzed by atmospheric-pressure ionization MS with electrospray ionization. (degruyter.com)
  • Separated analytes were determined in LC-MS single ion monitoring mode using an atmospheric pressure ionization-electrospray ionization (ESI) interface. (erowid.org)