• secretion
  • Studies using isolated preparations of thick ascending limb with glomerulus attached in low NaCl perfusate were unable to inhibit renin secretion when various sodium salts were added but could inhibit renin secretion with the addition of chloride salts. (wikipedia.org)
  • NHE1
  • B) Example of a recording set for NHE1 transport activity in a single CHO cell using different bath sodium concentrations at fixed extracellular (8.2) and intracellular pH (6.0). (nih.gov)
  • membrane
  • The graphs show sodium jump-induced peak currents using ΔP45-N58 NhaA RSO proteoliposomes (A and B), ΔP45-N58 NhaA ISO membrane vesicles (C and D), H225R NhaA RSO proteoliposomes (E and F), and A167P RSO proteoliposomes (G and H). Data and conditions are as in Fig. 2 (pH dependences) and Table 1 (Na+ dependences). (nih.gov)
  • mechanism
  • 1 An increase in the abundance of NHE-1 protein in cultured VSMCs from the SHR seems a likely mechanism for such an alteration and one that could also explain the elevated V max of the Na + -H + antiporter. (ahajournals.org)
  • While the exact mechanism is unknown, it is speculated that inhibition of this pump reduces sodium and calcium overload in cells of Duchenne patients. (wikipedia.org)
  • concentrations
  • Simultaneous fit of the pH dependences at indicated sodium concentrations and the sodium dependences at indicated pH values to the kinetic model. (nih.gov)
  • cells
  • Na(+)/H(+) antiporters show a marked pH dependence, which is important for their physiological function in eukaryotic and prokaryotic cells. (nih.gov)
  • Cultured vascular smooth muscle cells (VSMCs) from the spontaneously hypertensive rat (SHR) exhibit increased activity of the Na + -H + antiporter as compared to normotensive Wistar-Kyoto (WKY) rats. (ahajournals.org)
  • 1 2 Increased activity of the Na + -H + antiporter in cultured VSMCs from the SHR likewise is observed in early passaged cells (passages 2 through 6) but not in cells grown in late subpassages (passages 7 through 10). (ahajournals.org)
  • rats
  • We conclude that the increase in the V max of the Na + -H + antiporter in cultured VSMCs from the SHR, compared to those from WKY rats, is due, at least in part, to increased levels of NHE-1 protein. (ahajournals.org)
  • 1 2 3 Analysis of the kinetic properties of the Na + -H + antiporter in cultured VSMCs from the SHR has revealed that the V max of the Na + -H + antiporter is increased as compared to VSMCs from WKY rats. (ahajournals.org)
  • 1 2 3 In contrast, thymic lymphocytes from the SHR display similar kinetic properties for the Na + -H + antiporter as compared to those from WKY rats. (ahajournals.org)
  • activity
  • This finding suggests that the greater level of Na + -H + antiporter activity in cultured VSMCs from the SHR is due to a posttranscriptional alteration. (ahajournals.org)
  • transport
  • Differential effects of mutations on the transport properties of the Na+/H+ antiporter NhaA from Escherichia coli. (nih.gov)
  • Cell
  • The sodium within the tubule cell may then be retained by the body rather than excreted in the urine. (wikipedia.org)
  • 4 These discordant findings may reflect cell-specific differences in the properties of the Na + -H + antiporter or, more likely, differences in the phenotype expressed in cultured VSMCs as compared to freshly isolated lymphocytes. (ahajournals.org)
  • specific
  • In NhaA, the Escherichia coli Na(+)/H(+) antiporter, specific single site mutations modulating the pH profile of the transporter have been described in the past. (nih.gov)