AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and Processes
Sirtuin 1Sirtuin 3Sirtuin 2SirtuinsStilbenesNaphtholsAcetylationGroup III Histone DeacetylasesNADEnzyme ActivatorsCaloric RestrictionNicotinamide PhosphoribosyltransferaseO-Acetyl-ADP-RiboseLongevityHistone DeacetylasesNiacinamideFibroblast Growth Factor 6Cell AgingMetabolic DiseasesSilent Information Regulator Proteins, Saccharomyces cerevisiaeAgingAcetate-CoA LigaseAcrylamidesHeterocyclic Compounds with 4 or More RingsMonocyte-Macrophage Precursor CellsMitochondriaLysineOxidative StressMitochondrial ProteinsBenzamidesNeurodegenerative DiseasesForkhead Transcription FactorsEnergy MetabolismGene Expression RegulationSignal TransductionProtein Processing, Post-TranslationalMice, Inbred C57BLGene Knockdown TechniquesHistonesAMP-Activated Protein Kinases
SIRT1ProteinsSIRT3GenesSir2pResveratrolYeastRegulateOxidative stressMitochondriaSir2TranscriptionalCellularRegulatorsModulatorsEnzymesEncodesActivatorsHistone deacetylasesTargetsPathwaysLifespanRegulatesGeneFamilyMammalsWestern Blot2021FoundActivityResearchersPhosphatidylinositol-3 kinaActivateApoptosisTherapeuticMolecularCell
SIRT119
  • Although there are seven mammalian sirtuins (SIRT1-7), little is known about their expression in the aging brain. (frontiersin.org)
  • Detects human Sirtuin 1/SIRT1 in direct ELISAs and Western blots. (rndsystems.com)
  • Sirtuin 1/SIRT1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Sirtuin 1/SIRT1 knockout HeLa cell line. (rndsystems.com)
  • Detection of Human Sirtuin 1/SIRT1 by Western Blot. (rndsystems.com)
  • PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). (rndsystems.com)
  • A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated). (rndsystems.com)
  • Detection of Sirtuin 1/SIRT1 in HepG2 Human Cell Line by Flow Cytometry. (rndsystems.com)
  • HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714, filled histogram) or isotype control antibody (Catalog # 5-001-A , open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127 ). (rndsystems.com)
  • Western Blot Shows Human Sirtuin 1/SIRT1 Specificity by Using Knockout Cell Line. (rndsystems.com)
  • A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. (rndsystems.com)
  • Background: Sirtuin 1 (Sirt1) and sirtuin 3 (Sirt3) participate in the regulation of lipid metabolism. (edu.pl)
  • A dual luciferase reporter assay was used to confirm the binding of miR‑132 and sirtuin 1 (SIRT1). (spandidos-publications.com)
  • Sirtuin 1 (SIRT1), a member of the sirtuin family, regulates the cellular ageing process, participates in metabolic diseases, such as diabetes, and is related to cancer development ( 13 ). (spandidos-publications.com)
  • Recent studies suggest that sirtuin 1 (SIRT1) has the potential to be a marker for undernutrition. (researchgate.net)
  • Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. (prosci-inc.com)
  • Resveratrol activated a protein in the sirtuin family (SIRT1), which then stimulated the activity of another protein involved in mitochondrial function. (lifeextension.com)
  • A primary product of the SIRT1 gene is SIRT1 ("the sirtuin protein"), hence the gene's name. (nutritionaloutlook.com)
  • 8 SIRT1/sirtuin is also reported to play a crucial role in metabolic homeostasis and is known to extend the lifespan of some model organisms, suggesting it may also help extend human lifespan. (nutritionaloutlook.com)
  • Quantitative real-time polymerase chain reactions were used to assess levels of SIRT1-3 and downstream targets in post mortem brain tissue from HD patients and control cases as well as after selective hypothalamic expression of mutant huntingtin (HTT) using recombinant adeno-associated viral vectors in mice. (lu.se)
Proteins11
  • SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. (wikipedia.org)
  • The human sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance, and metabolic regulation. (wikipedia.org)
  • Yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. (wikipedia.org)
  • In addition to protein deacetylation, studies have shown that the human sirtuins may also function as intracellular regulatory proteins with mono ADP ribosyltransferase activity. (wikipedia.org)
  • The presence of the sirtuin deacetylase SIRT3 in the mitochondrial matrix suggests the existence of lysine acetylated mitochondrial proteins. (wikipedia.org)
  • Sirtuins are a group of proteins. (mindbodygreen.com)
  • Sirtuin 7 is a member of the sirtuin family of proteins . (bvsalud.org)
  • The mammalian sirtuin (SIRT) family, evolutionally conserved proteins belonging to class III histone deacetylases (HDACs), comprises seven members. (biomedcentral.com)
  • Other recent studies, including one conducted at the Joslin Diabetes Center, have found another member of the sirtuin family of cellular proteins that may play a major role in how fat is produced and stored, offering a new target for treatments to prevent obesity and reduce the risk of type 2 diabetes. (lifeextension.com)
  • Silent information regulator 2 (Sir2) proteins, or sirtuins, are protein deacetylases dependent on nicotine adenine dinucleotide (NAD) and are found in organisms ranging from bacteria to humans. (biomedcentral.com)
  • these Sir2 proteins, also called sirtuins, are the focus of this article. (biomedcentral.com)
SIRT34
  • Three sirtuins, SIRT3, SIRT4 and SIRT5, are located in mitochondria and have been implicated in regulating metabolic processes. (wikipedia.org)
  • Sirtuin-3 (SIRT3) performs a vital role in regulating metabolism, mitochondrial function, and oxidative stress. (mdpi.com)
  • In this study, we found that sirtuin 3 (SIRT3), a mitochondrial protein deacetylase, plays a critical role in host defense against Mabc infection. (gnu.ac.kr)
  • A protein called sirtuin 3-SIRT3-is critical to the process, the researchers say. (futurity.org)
Genes5
  • Generally, the regulation of target genes by miRNAs occurs through binding with the 3′-untranslated region (UTR). (spandidos-publications.com)
  • There are seven genes in a certain family called sirtuins that respond to the environment and protect the epigenome. (goop.com)
  • Being hungry every once in a while, intermittent fasting, exercising, or losing our breath makes the sirtuin genes more active. (goop.com)
  • On the heels of promising data showing that Sirt6 extends the median lifespan of male and female mice by 27% and 15%, a new study shows that Sirt6, one of seven sirtuin genes, also increases longevity in flies, suggesting that Sirt6's role in regulating lifespan is conserved across several species in the animal kingdom. (nad.com)
  • In the present study, Taylor and colleagues manually overexpress one of the sirtuin genes - Sirt6 - in male and female flies. (nad.com)
Sir2p1
  • While Sir2p homologues (sirtuins) have been found in all eukaryotes examined, Sir3p and Sir4p are restricted to close relatives of S. cerevisiae . (biomedcentral.com)
Resveratrol3
  • 3 The following year the researchers went on to demonstrate that resveratrol slows aging in two standard laboratory animals, roundworms and fruit flies. (lifeextension.com)
  • 6 Since the study found a link between sirtuins and energy utilization, the researchers concluded that resveratrol may be helpful in the prevention and treatment of certain metabolic disorders, especially those related to mitochondrial dysfunction, such as Alzheimer's and Parkinson's disease-two neurodegenerative conditions that become more prevalent with aging. (lifeextension.com)
  • 2017). Sirtuin 1-dependent resveratrol cytotoxicity and pro-differentiation activity on breast cancer cells . (up.pt)
Yeast2
  • Sirtuins were originally discovered in yeast for its role in prolonging replicative lifespan . (bvsalud.org)
  • Thus the budding yeast Saccharomyces cerevisiae sports TG 1-3 repeats on the ends of its chromosomes [ 2 ], while telomeres of both the filamentous fungus Neurospora crassa and humans comprise TTAGGG repeats [ 3 , 4 ]. (biomedcentral.com)
Regulate3
  • The key question is whether sirtuins regulate healthier aging in mammals. (frontiersin.org)
  • Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. (prosci-inc.com)
  • In addition to the catalytic domain, eukaryotic sirtuins contain variable amino- and carboxy-terminal extensions that regulate their subcellular localizations and catalytic activity. (biomedcentral.com)
Oxidative stress3
  • Myocardial I/R injury may induce cell apoptosis and autophagy by activating oxidative stress and upregulating inflammatory mediators, ultimately resulting in irreversible fibrotic damage ( 3 ). (spandidos-publications.com)
  • Elevated sirtuin - enzymes that promote longevity -activity, specifically Sirt6 (Sirtuin 6) increases lifespan by 33% in male flies and 38% in female flies and strengthens resistance to oxidative stress. (nad.com)
  • These initial findings suggest that sirtuins facilitate increased lifespan by blocking oxidative stress-induced cellular damage. (nad.com)
Mitochondria5
Sir22
  • The Sir2 catalytic domain, which is shared among all sirtuins, consists of two distinct domains that bind NAD and the acetyl-lysine substrate, respectively. (biomedcentral.com)
  • Mutation of three additional SIR2 homologues, nst-2 , nst-3 and nst-5 , partially relieved silencing. (biomedcentral.com)
Transcriptional1
  • In addition to controlling metabolism at the transcriptional level, sirtuins also directly control the activity of metabolic enzymes. (wikipedia.org)
Cellular9
  • This study identifies previously unknown roles for sirtuins in regulating cellular homeostasis and healthy aging. (frontiersin.org)
  • Mammalian sirtuins have diverse locations and multiple targets, and affect a broad range of cellular processes (Supplementary Figure 1). (frontiersin.org)
  • Sirtuins Are Critical For Cellular Health - What Are They & How Do They Work With NAD? (mindbodygreen.com)
  • Sirtuins play an important role in managing healthy cellular functioning," explains Kate Denniston, N.D., a licensed naturopathic doctor and founder of Los Angeles Integrative Health . (mindbodygreen.com)
  • In other words, each sirtuin specializes in regulating unique (and crucial) cellular functions. (mindbodygreen.com)
  • Why are sirtuins so critical for cellular health? (mindbodygreen.com)
  • When it comes to the game of cellular aging, sirtuins are like the referees. (mindbodygreen.com)
  • In turn, sirtuins affect "your cellular health across a wide variety of organs and systems," says Vikki Petersen , D.C., CCN, CFMP, certified functional medicine practitioner and founder of Root Cause Medical Clinic . (mindbodygreen.com)
  • For starters, sirtuins help repair DNA damage 3 , the main cause of cellular senescence. (mindbodygreen.com)
Regulators2
  • Sirtuins or "silent information regulators" of gene transcription, are a family of enzymes which are expressed throughout all phyla of life. (frontiersin.org)
  • The aim of this study was to investigate if the metabolic regulators sirtuins are affected in HD. (lu.se)
Modulators2
  • Furthermore, recent interest focusing on sirtuin modulators as epigenetic players in the regulation of fundamental biological pathways has prompted increased efforts to discover new small molecules able to modify sirtuin activity. (biomedcentral.com)
  • Here, we provide a summary of the most important SIRT activities and SIRT modulators, their latest analogs, and novel chemotypes (Tables 2 and 3 ). (biomedcentral.com)
Enzymes2
Encodes1
Activators3
  • Here, we review the role, mechanism of action, and biological function of the seven sirtuins, as well as their inhibitors and activators. (biomedcentral.com)
  • The dietary activators of AMPK include following a balanced anti-inflammatory calorie-restricted diet such as the Zone diet, coupled with the adequate intake of omega-3 fatty acids and sufficient consumption of polyphenols. (drsears.com)
  • Since AMPK is the master regulator of metabolism, reducing its dietary inhibitors (excess calories and excess glucose) while simultaneously increasing its dietary activators (the Zone diet, omega-3 fatty acids, and polyphenols) represents a new theory for obesity. (drsears.com)
Histone deacetylases1
  • Sirtuins are NAD + -dependent histone deacetylases regulating important metabolic pathways in prokaryotes and eukaryotes and are involved in many biological processes such as cell survival, senescence, proliferation, apoptosis, DNA repair, cell metabolism, and caloric restriction. (biomedcentral.com)
Targets3
  • However, metabolic dysregulated factors such as peroxisome proliferator-activated receptors and sirtuins may serve as therapeutic targets to control this response by mitigating both Toll-like receptors and inflammasome signaling. (hindawi.com)
  • Guarente L. Sirtuins as potential targets for metabolic syndrome. (prosci-inc.com)
  • In the field of aging and age-related diseases, there is increasing evidence that sirtuins may provide novel targets for treating diseases associated with aging. (nutritionaloutlook.com)
Pathways2
  • Induction of canonical autophagy is mainly controlled by the AKT/mTOR and AMPK signaling pathways and relies on the activation and assembly of two macromolecular complexes, the ATG1/ULK1 and the Class III phosphatidylinositol 3-kinase (PI3K) complex (Figure 1 ) [ 5 ]. (hindawi.com)
  • Bellegarrigue NA, Velásquez factor, and sirtuin enzyme pathways are considered, generating a neuroprotective effect. (bvsalud.org)
Lifespan2
  • However, the extent to which sirtuins contribute to prolonging lifespan across species is still not clear. (nad.com)
  • Upon doing so, the Brown researchers found that increasing sirtuin levels increased lifespan by 33% in male flies and 38% in female flies. (nad.com)
Regulates1
  • In Salmonella enterica, the bacterial sirtuin CobB regulates the activity of the enzyme acetyl-coenzyme A (acetyl-CoA) synthetase. (wikipedia.org)
Gene2
  • Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes, and the protein encoded by this gene is included in class I of the sirtuin family. (wikipedia.org)
  • Specifically, the sirtuin-3 gene gets activated by cold, which promotes the browning of fat, which we believe is good for us. (goop.com)
Family3
  • You can think of sirtuins as a family whose members are physically similar but have different talents. (mindbodygreen.com)
  • The hallmark of the family is a domain of approximately 260 amino acids that has a high degree of sequence similarity in all sirtuins. (biomedcentral.com)
  • The family is divided into five classes (I-IV and U) on the basis of a phylogenetic analysis of 60 sirtuins from a wide array of organisms [ 17 ] (Figure 1 ). (biomedcentral.com)
Mammals1
Western Blot1
  • a - c The expression of Ki67, Bcl-2, Bax, and cleaved Caspase-3 in MH7A cells was detected by western blot. (biomedcentral.com)
20211
  • 2021 Mar 1;218(3):e20202135. (nih.gov)
Found1
Activity2
  • What's more, "changes in NAD+ levels affect sirtuin activity," shares Denniston. (mindbodygreen.com)
  • It plays an important role in mitochondrial function and in antioxidant response in lots of tissues in the body, so the idea that EGCG might selectively affect the activity of sirtuin 3 in cancer cells-to turn it off-and in normal cells-to turn it on-is probably applicable in multiple kinds of cancers," Lambert says. (futurity.org)
Researchers2
  • Earlier studies showed the compound called epigallocatechin-3-gallate (EGCG) killed oral cancer cells, but researchers didn't how it worked, says Joshua Lambert, associate professor of food science at Penn State University. (futurity.org)
  • Given that sirtuin abundance naturally declines with age, researchers are continuously looking for ways to replenish these life-preserving compounds. (nad.com)
Phosphatidylinositol-3 kina1
  • We have previously shown that GLP-1 transactivates the epidermal growth factor receptor ( 12 ) to subsequently activate phosphatidylinositol-3 kinase and Akt signaling ( 7 , 11 ). (diabetesjournals.org)
Activate2
  • And we've shown that when we activate these sirtuins, either with lifestyle or natural molecules, we get improved health and longevity in old age. (goop.com)
  • Finally, you must consume adequate polyphenols that indirectly activate AMPK by stimulating sirtuins ( 7 ). (drsears.com)
Apoptosis1
  • DCM entails the damage of the myocardium through fibrosis, steatosis, apoptosis, and hypertrophy [ 3 ] and results from the switch of substrate supply to free fatty acids (FFA) that follows the reduced levels of insulin, glucose transporters, and glucose consumption [ 4 , 5 ]. (hindawi.com)
Therapeutic1
  • The glucoincretin hormone glucagon-like peptide 1-[7-36]amide (GLP-1) ( 1 - 3 ) is a potent therapeutic agent in the treatment of diabetes ( 4 ). (diabetesjournals.org)
Molecular1
Cell3
  • Sirtuin 7 in cell proliferation, stress and disease: Rise of the Seventh Sirtuin! (bvsalud.org)
  • Cell Signal;27(3): 673-82, 2015 Mar. (bvsalud.org)
  • There are primarily two types of lung cancer: Non-small cell lung cancer (NSCLC) and SCLC, and the former accounts for ~85% of all lung cancer cases ( 3 ). (spandidos-publications.com)