• demonstrate
  • Collectively, our results demonstrate that anti-siderophore antibodies inhibit Salmonella colonization. (universia.net)
  • Combined with the absence of a sidA ortholog-and the fungal siderophore system in general-in mammals, these data demonstrate that the siderophore biosynthetic pathway represents a promising new target for the development of antifungal therapies. (rupress.org)
  • We reconstituted the reaction in vitro with the main enzyme AvbD and the putative biosynthetic precursors, and demonstrate that multispecificity of this enzyme results in the production of all three cyclic hydroxamate siderophores that were previously isolated as natural products from S. algae. (uni-konstanz.de)
  • In live cells, however, siderophore ratios are maximized toward avaroferrin production, and we demonstrate that these siderophore ratios are the result of a regulation on substrate pool level, which may allow rapid evolutionary adaptation to environmental changes. (uni-konstanz.de)
  • FhuA
  • Siderophore transport through Escherichia coli outer membrane receptor FhuA with disulfide-tethered cork and barrel domains. (semanticscholar.org)
  • In vivo synthesis of the periplasmic domain of TonB inhibits transport through the FecA and FhuA iron siderophore transporters of Escherichia coli. (semanticscholar.org)
  • It can be seen in crystal structures of the related FhuA protein bound to its siderophore ( 8 , 9 ) that the siderophore binds to sites where it contacts some of the outer loops as well as several residues from the N-terminal plug domain. (pnas.org)
  • Crystal structures of FhuA with and without its siderophore bound show very little change near the binding site but large changes at the N terminus of the protein, 30 Å away on the inner face of the membrane. (pnas.org)
  • inhibit
  • In the study, S. maltophilia 15 showed algicidal activities by secreting siderophore and could inhibit cyanobacterial growth, especially when iron bioavailabity is very low. (genscript.com)
  • Salmonella
  • Mice immunized with the CTB-siderophore conjugate developed anti-siderophore antibodies in the gut mucosa, and whenmice were infected with the enteric pathogen Salmonella, they exhibited reduced intestinal colonization and reduced systemic dissemination of the pathogen. (universia.net)
  • molecules
  • Here, we report an immunization approach that targets siderophores, which are small molecules exported by enteric Gram-negative pathogens to acquire iron, an essential nutrient, in the host. (universia.net)
  • Siderophores are small molecules used by microbes to acquire the essential metal Fe in environments ranging from infections to the open ocean. (pnas.org)
  • The secretion of small Fe-binding molecules called siderophores is an important microbial strategy for survival in Fe-limited environments. (pnas.org)
  • transporter
  • In Candida albicans, the siderophore transporter Arn1p/Sit1p is required for epithelial invasion and penetration but not for systemic infection ( 13 ). (rupress.org)
  • FepA, an outer membrane iron siderophore transporter from Escherichia coli , is composed of a 22-stranded membrane-spanning β barrel with a globular N-terminal "plug" domain of 148 residues that folds up inside the barrel and completely occludes the barrel's interior (1). (pnas.org)
  • chelation therapy
  • Because of this property, they have attracted interest from medical science in metal chelation therapy, with the siderophore desferrioxamine B gaining widespread use in treatments for iron poisoning and thalassemia. (wikipedia.org)
  • deferoxamine, a naturally occurring siderophore produced by the actinobacter Streptomyces pilosus and was used initially as a chelation therapy agent. (wikipedia.org)
  • Aspergillus
  • Eisendle, M., Oberegger, H., Zadra, I. and Haas, H. (2003) The Siderophore System Is Essential for Viability of Aspergillus nidulans Functional Analysis of Two Genes Encoding L-Ornithine N5-Monooxygenase (sidA) and a Non-Ribosomal Peptide Synthetase (sidC). (scirp.org)
  • ions
  • Siderophores usually form a stable, hexadentate, octahedral complex preferentially with Fe3+ compared to other naturally occurring abundant metal ions, although if there are fewer than six donor atoms water can also coordinate. (wikipedia.org)
  • vitro
  • However, recent data indicate that A. fumigatus survival in human serum in vitro involves siderophore-mediated removal of iron from transferrin ( 17 ). (rupress.org)
  • Our results show that it is crucial to develop defined in vitro models in order to predict whether siderophores are social, cheatable and suitable for clinical exploitation in specific infection contexts. (nottingham.ac.uk)